LOCUS       NM_022414               1630 bp    mRNA    linear   ROD 05-AUG-2024
DEFINITION  Mus musculus neuroglobin (Ngb), transcript variant 2, mRNA.
ACCESSION   NM_022414
VERSION     NM_022414.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Cwerman-Thibault,H., Malko-Baverel,V., Le Guilloux,G.,
            Ratcliffe,E., Mouri,D., Torres-Cuevas,I., Millan,I., Saubamea,B.,
            Mignon,V., Boespflug-Tanguy,O., Gressens,P. and Corral-Debrinski,M.
  TITLE     Neuroglobin overexpression in cerebellar neurons of Harlequin mice
            improves mitochondrial homeostasis and reduces ataxic behavior
  JOURNAL   Mol Ther 32 (7), 2150-2175 (2024)
   PUBMED   38796706
  REMARK    GeneRIF: Neuroglobin overexpression in cerebellar neurons of
            Harlequin mice improves mitochondrial homeostasis and reduces
            ataxic behavior.
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Gotzsche,C.R., Woldbye,D.P.D., Hundahl,C.A. and Hay-Schmidt,A.
  TITLE     Neuroglobin deficiency increases seizure susceptibility but does
            not affect basal behavior in mice
  JOURNAL   J Neurosci Res 100 (10), 1921-1932 (2022)
   PUBMED   35822521
  REMARK    GeneRIF: Neuroglobin deficiency increases seizure susceptibility
            but does not affect basal behavior in mice.
REFERENCE   3  (bases 1 to 1630)
  AUTHORS   Exertier,C., Sebastiani,F., Freda,I., Gugole,E., Cerutti,G.,
            Parisi,G., Montemiglio,L.C., Becucci,M., Viappiani,C., Bruno,S.,
            Savino,C., Zamparelli,C., Anselmi,M., Abbruzzetti,S., Smulevich,G.
            and Vallone,B.
  TITLE     Probing the Role of Murine Neuroglobin CDloop-D-Helix Unit in CO
            Ligand Binding and Structural Dynamics
  JOURNAL   ACS Chem Biol 17 (8), 2099-2108 (2022)
   PUBMED   35797699
  REMARK    GeneRIF: Probing the Role of Murine Neuroglobin CDloop-D-Helix Unit
            in CO Ligand Binding and Structural Dynamics.
REFERENCE   4  (bases 1 to 1630)
  AUTHORS   Pereira Luppi,M., Azcorra,M., Caronia-Brown,G., Poulin,J.F.,
            Gaertner,Z., Gatica,S., Moreno-Ramos,O.A., Nouri,N., Dubois,M.,
            Ma,Y.C., Ramakrishnan,C., Fenno,L., Kim,Y.S., Deisseroth,K.,
            Cicchetti,F., Dombeck,D.A. and Awatramani,R.
  TITLE     Sox6 expression distinguishes dorsally and ventrally biased
            dopamine neurons in the substantia nigra with distinctive
            properties and embryonic origins
  JOURNAL   Cell Rep 37 (6), 109975 (2021)
   PUBMED   34758317
REFERENCE   5  (bases 1 to 1630)
  AUTHORS   Rose,J.J., Bocian,K.A., Xu,Q., Wang,L., DeMartino,A.W., Chen,X.,
            Corey,C.G., Guimaraes,D.A., Azarov,I., Huang,X.N., Tong,Q., Guo,L.,
            Nouraie,M., McTiernan,C.F., O'Donnell,C.P., Tejero,J., Shiva,S. and
            Gladwin,M.T.
  TITLE     A neuroglobin-based high-affinity ligand trap reverses carbon
            monoxide-induced mitochondrial poisoning
  JOURNAL   J Biol Chem 295 (19), 6357-6371 (2020)
   PUBMED   32205448
  REMARK    GeneRIF: A neuroglobin-based high-affinity ligand trap reverses
            carbon monoxide-induced mitochondrial poisoning.
REFERENCE   6  (bases 1 to 1630)
  AUTHORS   Kriegl,J.M., Bhattacharyya,A.J., Nienhaus,K., Deng,P., Minkow,O.
            and Nienhaus,G.U.
  TITLE     Ligand binding and protein dynamics in neuroglobin
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7992-7997 (2002)
   PUBMED   12048231
  REMARK    GeneRIF: ligand binding and protein dynamics
REFERENCE   7  (bases 1 to 1630)
  AUTHORS   Couture,M., Burmester,T., Hankeln,T. and Rousseau,D.L.
  TITLE     The heme environment of mouse neuroglobin. Evidence for the
            presence of two conformations of the heme pocket
  JOURNAL   J Biol Chem 276 (39), 36377-36382 (2001)
   PUBMED   11473111
  REMARK    GeneRIF: Resonance Raman spectroscopy was used to determine the
            structure of the heme environment in Ngb from mice.
REFERENCE   8  (bases 1 to 1630)
  AUTHORS   Awenius,C., Hankeln,T. and Burmester,T.
  TITLE     Neuroglobins from the zebrafish Danio rerio and the pufferfish
            Tetraodon nigroviridis
  JOURNAL   Biochem Biophys Res Commun 287 (2), 418-421 (2001)
   PUBMED   11554744
REFERENCE   9  (bases 1 to 1630)
  AUTHORS   Gysemans,C.A., Waer,M., Valckx,D., Laureys,J.M., Mihkalsky,D.,
            Bouillon,R. and Mathieu,C.
  TITLE     Early graft failure of xenogeneic islets in NOD mice is accompanied
            by high levels of interleukin-1 and low levels of transforming
            growth factor-beta mRNA in the grafts
  JOURNAL   Diabetes 49 (12), 1992-1997 (2000)
   PUBMED   11117999
REFERENCE   10 (bases 1 to 1630)
  AUTHORS   Burmester,T., Weich,B., Reinhardt,S. and Hankeln,T.
  TITLE     A vertebrate globin expressed in the brain
  JOURNAL   Nature 407 (6803), 520-523 (2000)
   PUBMED   11029004
  REMARK    GeneRIF: The paper describes the discovery of neuroglobin, the
            oxygen-binding properties (P50 = 1-2 Torr), and its preferential
            expression in the mouse brain.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC024263.1.
            
            On Jun 9, 2003 this sequence version replaced NM_022414.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1. This
            results in a shorter protein (isoform 2), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024263.1, SRR1660813.195480.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="FVB/N"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 41.29 cM"
     gene            1..1630
                     /gene="Ngb"
                     /note="neuroglobin"
                     /db_xref="GeneID:64242"
                     /db_xref="MGI:MGI:2151886"
     exon            1..368
                     /gene="Ngb"
                     /inference="alignment:Splign:2.1.0"
     CDS             280..735
                     /gene="Ngb"
                     /note="isoform 2 is encoded by transcript variant 2;
                     nitrite reductase"
                     /codon_start=1
                     /product="neuroglobin isoform 2"
                     /protein_id="NP_071859.1"
                     /db_xref="CCDS:CCDS36501.1"
                     /db_xref="GeneID:64242"
                     /db_xref="MGI:MGI:2151886"
                     /translation="MERPESELIRQSWRVVSRSPLEHGTVLFARLFALEPSLLPLFQY
                     NGRQFSSPEDCLSSPEFLDHIRKVMLVIDAAVTNVEDLSSLEEYLTSLGRKHRAVGVR
                     LSSFSTVGESLLYMLEKCLGPDFTPATRTAWSRLYGAVVQAMSRGWDGE"
     exon            369..480
                     /gene="Ngb"
                     /inference="alignment:Splign:2.1.0"
     exon            481..600
                     /gene="Ngb"
                     /inference="alignment:Splign:2.1.0"
     exon            601..1616
                     /gene="Ngb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1594..1599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ngb"
                     /note="hexamer: AATAAA"
     polyA_site      1616
                     /gene="Ngb"
                     /note="major polyA site"
ORIGIN      
        1 gccaccgtag ctttaatggg cggttctctg ggagcttcgg ggtgtagggc gcagctgccc
       61 aagcggggtc cccggaagcg cccgaggcca agctggccgt gcgcatcctc tgggctggac
      121 cagtaaaggc gcagttgctg ggccccaccc aactaaggca ctgggcctgg aggggggctc
      181 ccccgcgtcg ccgaggagat ggcgctgcat gtgcgttgac tgcacccacg cctcgagggt
      241 cccatcactg cgtcccgcga gtctcctggg agagagagca tggagcgccc ggagtcagag
      301 ctgatccggc agagctggcg ggtagtgagc cgcagccctc tggaacatgg cactgtcctg
      361 ttcgccaggc tcttcgccct ggaacccagc ctgctgcctc tcttccagta caatggccgc
      421 cagttctcca gccctgagga ctgtctctcc tctccagaat tcctggacca cattaggaag
      481 gtgatgctag tgattgatgc tgcagtgacc aacgtggagg acctgtcttc attggaggag
      541 tacctgacca gcttgggcag gaagcatcgg gcagtgggag tgaggctcag ctccttctcg
      601 acagtaggcg agtccctgct ctacatgctg gagaagtgcc tgggtcccga ctttacacca
      661 gctacaagga ccgcctggag ccgactctac ggagctgtgg tgcaagccat gagccgaggc
      721 tgggatgggg agtaagagac gagccagtgc ccctatctat gtgtgtctgt ctgttgatct
      781 gcctgttgta gtcttagcct ctcccccagg gtctctctat accttggtcc tcatcccctt
      841 ggccatccta gagaggtgat gggagagggc tctgtctcag cttctggtca gctgcaggga
      901 ggtggatttc cttccctcat ccccatagat gtcctgctgc atgcctggtt agctcgcccc
      961 aggaggcaga acaatttggg tagtgaggtg gcatgggggt tcttctcaga gaagctttct
     1021 gccaaggtga gggaaaagtg actaggcttc tagaattttc caccttgcct gcccagtctc
     1081 tctgatactt ccctgccaag ctgtttactt tccaacaatg aagaggagag actttttagt
     1141 gtttctcagg caagggaagc atagaagatg gcattcttca gtggcattgg gcagtggctc
     1201 agctgctcag acgccccata caaaacctct tgtgctcttg gttcagcatt tctggtggaa
     1261 gcaccccggt attcttttgg gggaaaccta actggccagc cataatggag aggccagaag
     1321 ggcaaggagg aggagtgacc tcatcccaaa tggcatggaa ttctggggct cttcctgcca
     1381 cttaattgta gaagggctga atcctgtctc cccaccctct tctgctcacc caccgctctc
     1441 cagctgtggg cccctagctc tatgtttaat gcttgaggtg tactcgtgcc tgggctgatg
     1501 agtgggagag aatagagtag ggagggccct tgtacccatc cccagccacc cacactttct
     1561 tgctctgtgc tgtggctcta taaactcaca agaaataaac atgttctgtg tgctgaaaaa
     1621 aaaaaaaaaa
//
