LOCUS       NM_010911               2053 bp    mRNA    linear   ROD 27-DEC-2022
DEFINITION  Mus musculus nitrogen fixation gene 1 (S. cerevisiae) (Nfs1), mRNA.
ACCESSION   NM_010911
VERSION     NM_010911.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Alvarez SW, Sviderskiy VO, Terzi EM, Papagiannakopoulos T, Moreira
            AL, Adams S, Sabatini DM, Birsoy K and Possemato R.
  TITLE     NFS1 undergoes positive selection in lung tumours and protects
            cells from ferroptosis
  JOURNAL   Nature 551 (7682), 639-643 (2017)
   PUBMED   29168506
  REMARK    GeneRIF: NFS1 maintains the iron sulfur clusters in proteins that
            are essential for protecting them from oxidative damage;
            inactivating NFS1 or iron sulfur clusters can trigger ferroptosis,
            a non-apoptotic form of cell death, in cancer cells
            Erratum:[Nature. 2022 Sep;609(7929):E12. PMID: 36104569]
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Boniecki MT, Freibert SA, Muhlenhoff U, Lill R and Cygler M.
  TITLE     Structure and functional dynamics of the mitochondrial Fe/S cluster
            synthesis complex
  JOURNAL   Nat Commun 8 (1), 1287 (2017)
   PUBMED   29097656
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2053)
  AUTHORS   Gakh O, Ranatunga W, Smith DY 4th, Ahlgren EC, Al-Karadaghi S,
            Thompson JR and Isaya G.
  TITLE     Architecture of the Human Mitochondrial Iron-Sulfur Cluster
            Assembly Machinery
  JOURNAL   J Biol Chem 291 (40), 21296-21321 (2016)
   PUBMED   27519411
REFERENCE   4  (bases 1 to 2053)
  AUTHORS   Parent A, Elduque X, Cornu D, Belot L, Le Caer JP, Grandas A,
            Toledano MB and D'Autreaux B.
  TITLE     Mammalian frataxin directly enhances sulfur transfer of NFS1
            persulfide to both ISCU and free thiols
  JOURNAL   Nat Commun 6, 5686 (2015)
   PUBMED   25597503
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2053)
  AUTHORS   Schmucker S, Martelli A, Colin F, Page A, Wattenhofer-Donze M,
            Reutenauer L and Puccio H.
  TITLE     Mammalian frataxin: an essential function for cellular viability
            through an interaction with a preformed ISCU/NFS1/ISD11 iron-sulfur
            assembly complex
  JOURNAL   PLoS One 6 (1), e16199 (2011)
   PUBMED   21298097
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2053)
  AUTHORS   Fosset C, Chauveau MJ, Guillon B, Canal F, Drapier JC and Bouton C.
  TITLE     RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity
            both in mitochondria and cytosol of mammalian cells
  JOURNAL   J Biol Chem 281 (35), 25398-25406 (2006)
   PUBMED   16787928
  REMARK    GeneRIF: there is a mechanism that primarily dedicates m-Nfs1 to
            the biogenesis of mitochondrial Fe-S clusters in order to maintain
            cell survival
REFERENCE   7  (bases 1 to 2053)
  AUTHORS   Biederbick A, Stehling O, Rosser R, Niggemeyer B, Nakai Y, Elsasser
            HP and Lill R.
  TITLE     Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein
            biogenesis and iron regulation
  JOURNAL   Mol Cell Biol 26 (15), 5675-5687 (2006)
   PUBMED   16847322
REFERENCE   8  (bases 1 to 2053)
  AUTHORS   Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl
            E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES and
            Mann M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 2053)
  AUTHORS   Nakai Y, Nakai M, Hayashi H and Kagamiyama H.
  TITLE     Nuclear localization of yeast Nfs1p is required for cell survival
  JOURNAL   J Biol Chem 276 (11), 8314-8320 (2001)
   PUBMED   11110795
REFERENCE   10 (bases 1 to 2053)
  AUTHORS   Nakai Y, Yoshihara Y, Hayashi H and Kagamiyama H.
  TITLE     cDNA cloning and characterization of mouse nifS-like protein,
            m-Nfs1: mitochondrial localization of eukaryotic NifS-like proteins
  JOURNAL   FEBS Lett 433 (1-2), 143-148 (1998)
   PUBMED   9738949
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132838.1 and AK004077.1.
            
            [WARNING] On May 2, 2023 this sequence was replaced by NM_010911.3.
            
            On Nov 24, 2007 this sequence version replaced NM_010911.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132838.1, AK148527.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2029              AK132838.1         2-2030
            2030-2053           AK004077.1         655-678
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..2053
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /note="nitrogen fixation gene 1 (S. cerevisiae)"
                     /db_xref="GeneID:18041"
                     /db_xref="MGI:MGI:1316706"
     exon            1..167
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1426
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /EC_number="2.8.1.7"
                     /note="nitrogen fixation gene, yeast homolog 1; cysteine
                     desulfurase, mitochondrial; nifS-like (sic)"
                     /codon_start=1
                     /product="cysteine desulfurase"
                     /protein_id="NP_035041.2"
                     /db_xref="CCDS:CCDS16962.1"
                     /db_xref="GeneID:18041"
                     /db_xref="MGI:MGI:1316706"
                     /translation="MVGSVAGNMLLRAAWRRASLAATSLALGRSSVPTRGLRLRVVDH
                     GPHSPVHSEAEAVLRPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESE
                     AAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKC
                     VLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIA
                     EIGQICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRP
                     RVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQN
                     IMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSY
                     VLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGID
                     LKSIKWTQH"
     exon            168..259
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            260..376
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            377..460
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            461..613
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            614..707
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            708..842
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1000
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1106
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1188
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1272
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1362
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..2053
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2016..2021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /note="putative"
     polyA_site      2029
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /note="putative"
     regulatory      2030..2035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /note="putative"
     polyA_site      2053
                     /gene="Nfs1"
                     /gene_synonym="m-Nfs1; m-Nfsl"
                     /note="putative"
ORIGIN      
        1 gcagttactt ccgggagcat gggctgagga cttgcaagcg ccgcggatgg tgggctctgt
       61 ggctgggaac atgctgctgc gagccgcttg gaggcgggcg tcgttggcgg ctacctcctt
      121 ggccctggga aggtcctcgg tgcccacccg gggactgcgc ctgcgcgttg tagatcatgg
      181 cccccattcg cctgttcact cagaggcaga ggcagtgctg cgacctctct acatggacgt
      241 acaggccacc actcctctgg atcccagagt gcttgatgcc atgctcccat accttgtcaa
      301 ctactatggg aaccctcatt ctcggactca tgcatatggc tgggagagcg aggcagccat
      361 ggaacgtgct cgccagcaag tagcatctct gattggagct gatcctcggg agatcatttt
      421 cactagtgga gctactgagt ccaacaacat agcaattaag ggagtggcca ggttctacag
      481 gtcaaggaaa aaacacttgg tcacaaccca gacagaacac aaatgtgtgc tggattcctg
      541 ccgctccctg gaagctgagg gctttcgggt cacttacctc cctgtgcaga agagcgggat
      601 cattgactta aaggaactag aggctgccat ccagccagac accagcctgg tctccgttat
      661 gactgtgaac aatgaaattg gagtaaagca accaattgca gaaatagggc agatttgtag
      721 ttccagaaag gtgtacttcc acactgatgc cgcccaagcg gttgggaaaa tcccacttga
      781 cgtcaatgac atgaagattg atctcatgag catcagcggt cacaaactct atggccctaa
      841 aggggttggt gctatctata tccgccgtag gccccgggta cgtgtggagg ccctacagag
      901 tggaggcggg caggagcggg gtatgcggtc tgggacagtg cccacaccct tggtggtggg
      961 cctgggagct gcatgtgagt tggcacaaca agagatggaa tacgaccata agcggatctc
     1021 aaagttagca gagcgactgg tacagaacat aatgaagaat cttccagatg tggtaatgaa
     1081 cggggacccg aagcagcact accctggctg tatcaacctc tccttcgcgt atgtggaagg
     1141 agagagcctg ctgatggccc tcaaggatgt ggccttgtcc tcagggagtg cctgcacttc
     1201 tgcatcattg gagccctctt atgtcctcag agcaattggt accgatgagg atttagcaca
     1261 ctcctctatc aggtttggca tcggccgctt cactacagaa gaggaggtgg actacacggc
     1321 ggagaagtgc atccaccacg tgaagcgcct tcgagaaatg agtcctctct gggagatggt
     1381 ccaggatggc attgacctca agagcatcaa gtggacccag cattagagga gtgggctgtg
     1441 actttgtgct ggcctggctg ttctggcctc actgatctgt gcacagccag gaacctcact
     1501 gcctgacgtg gtccatgctc aaagcagatg ggaagctctg cagtgacttc accacagaat
     1561 gttctctagt gaaagctgag gctagctttc ttagcctcgg ctcctgtgta ggcatgtatt
     1621 ccgtaatctt cctcaggagt tcttctcttg cctggcgtgg ccattcttat ctgagggcct
     1681 ggaggcactg ccttgttact gccacaggga ggacctacct gtgcagagat ggaagaactg
     1741 gaagaaggct ctaggttcaa agactgcttc tctgtgaatc attactctag ctgctcagct
     1801 gaactggcgc cattagggac tggcataaac aaactttatt ttaacctttt ttgtggcttt
     1861 gaaagacatc tctttgtatc gacttcagct gaagaacacc aagttgtatt aaagtcttgt
     1921 ttcctttggg gtctcatctt ctttcctctc ttttgactta atgtaagtag aaatatccat
     1981 gaccccaatc attattctaa taatttactt aattcattaa acttaattca ataaattccc
     2041 atttcctgtc ctg
//
