LOCUS       NM_001371055            5367 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus nuclear factor I/X (Nfix), transcript variant 8, mRNA.
ACCESSION   NM_001371055
VERSION     NM_001371055.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Caria,C.A., Faa,V., Porcu,S., Marongiu,M.F., Poddie,D., Perseu,L.,
            Meloni,A., Vaccargiu,S. and Ristaldi,M.S.
  TITLE     Post-GWAS Validation of Target Genes Associated with HbF and HbA2
            Levels
  JOURNAL   Cells 13 (14), 1185 (2024)
   PUBMED   39056767
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Kooblall,K.G., Stevenson,M., Heilig,R., Stewart,M., Wright,B.,
            Lockstone,H., Buck,D., Fischer,R., Wells,S., Lines,K.E., Teboul,L.,
            Hennekam,R.C. and Thakker,R.V.
  TITLE     Identification of cellular retinoic acid binding protein 2 (CRABP2)
            as downstream target of nuclear factor I/X (NFIX): implications for
            skeletal dysplasia syndromes
  JOURNAL   JBMR Plus 8 (7), ziae060 (2024)
   PUBMED   38827116
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5367)
  AUTHORS   Angelini,G., Capra,E., Rossi,F., Mura,G., Saclier,M., Taglietti,V.,
            Rovetta,G., Epis,R., Careccia,G., Bonfanti,C. and Messina,G.
  TITLE     MEK-inhibitors decrease Nfix in muscular dystrophy but induce
            unexpected calcifications, partially rescued with Cyanidin diet
  JOURNAL   iScience 27 (1), 108696 (2023)
   PUBMED   38205246
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5367)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5367)
  AUTHORS   Pan,D., Zhong,J., Zhang,J., Dong,H., Zhao,D., Zhang,H. and Yao,B.
  TITLE     Function and regulation of nuclear factor 1 X-type on chondrocyte
            proliferation and differentiation
  JOURNAL   Gene 881, 147620 (2023)
   PUBMED   37433356
  REMARK    GeneRIF: Function and regulation of nuclear factor 1 X-type on
            chondrocyte proliferation and differentiation.
REFERENCE   6  (bases 1 to 5367)
  AUTHORS   Fletcher,C.F., Lutz,C.M., O'Sullivan,T.N., Shaughnessy,J.D. Jr.,
            Hawkes,R., Frankel,W.N., Copeland,N.G. and Jenkins,N.A.
  TITLE     Absence epilepsy in tottering mutant mice is associated with
            calcium channel defects
  JOURNAL   Cell 87 (4), 607-617 (1996)
   PUBMED   8929530
REFERENCE   7  (bases 1 to 5367)
  AUTHORS   Kulkarni,S. and Gronostajski,R.M.
  TITLE     Altered expression of the developmentally regulated NFI gene family
            during phorbol ester-induced differentiation of human leukemic
            cells
  JOURNAL   Cell Growth Differ 7 (4), 501-510 (1996)
   PUBMED   9052991
REFERENCE   8  (bases 1 to 5367)
  AUTHORS   Qian,F., Kruse,U., Lichter,P. and Sippel,A.E.
  TITLE     Chromosomal localization of the four genes (NFIA, B, C, and X) for
            the human transcription factor nuclear factor I by FISH
  JOURNAL   Genomics 28 (1), 66-73 (1995)
   PUBMED   7590749
REFERENCE   9  (bases 1 to 5367)
  AUTHORS   Nebl,G. and Cato,A.C.
  TITLE     NFI/X proteins: a class of NFI family of transcription factors with
            positive and negative regulatory domains
  JOURNAL   Cell Mol Biol Res 41 (2), 85-95 (1995)
   PUBMED   8581067
REFERENCE   10 (bases 1 to 5367)
  AUTHORS   Scherthan,H., Seisenberger,C., Greulich,K. and Winnacker,E.L.
  TITLE     Mapping of the murine nuclear factor I/X gene (Nfix) to mouse
            chromosome 8 C1-2 by FISH
  JOURNAL   Genomics 22 (1), 247-249 (1994)
   PUBMED   7959784
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155163.4 and AC145556.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2224191.1,
                                           ERR3835353.287818.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AC155163.4         118014-118070       c
            58-589              AC155163.4         116444-116975       c
            590-652             AC155163.4         72565-72627         c
            653-727             AC155163.4         72265-72339         c
            728-848             AC155163.4         71784-71904         c
            849-985             AC155163.4         71129-71265         c
            986-1161            AC155163.4         66303-66478         c
            1162-1309           AC155163.4         60801-60948         c
            1310-1401           AC155163.4         58426-58517         c
            1402-2044           AC155163.4         52695-53337         c
            2045-5367           AC145556.4         175078-178400       c
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.02 cM"
     gene            1..5367
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="nuclear factor I/X"
                     /db_xref="GeneID:18032"
                     /db_xref="MGI:MGI:97311"
     exon            1..57
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1416
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="isoform 8 is encoded by transcript variant 8;
                     nuclear factor 1 X-type; nuclear factor 1/X; TGGCA-binding
                     protein; CCAAT-box-binding transcription factor"
                     /codon_start=1
                     /product="nuclear factor 1 X-type isoform 8"
                     /protein_id="NP_001357984.1"
                     /db_xref="GeneID:18032"
                     /db_xref="MGI:MGI:97311"
                     /translation="MDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDE
                     ERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQ
                     KGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQCSNPGLCVQPHHI
                     GVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHLSFQDCFVTSGVWNVTE
                     LVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSSTKRPKSIDDS
                     EMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGSPRATASALHFPSTSIIQQSSPY
                     FTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQPNGSGQGKVPGSFLLPPPPPVAR
                     PVPLPMPDSKTTSTAPDGAALTPPSPSFTTTGASSANRFVSIGSRDGNFLNIPQQSQS
                     WFL"
     exon            58..589
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            590..652
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            653..727
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            728..848
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            849..985
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1161
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1309
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1401
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..5367
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1536
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="major polyA site"
     regulatory      5347..5352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="hexamer: AATAAA"
     regulatory      5351..5356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="hexamer: AAGAAA"
     polyA_site      5362
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
     polyA_site      5367
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="major polyA site"
ORIGIN      
        1 gttgagccca gaatctcaga atcaggcgtt ggctttgccg ggtgcttcag atcaatggat
       61 gagttccacc cgtttatcga ggcgctgctg cctcacgtcc gagccttctc ctacacctgg
      121 ttcaacctgc aggcgcggaa gcgcaagtac ttcaagaagc acgagaagcg gatgtcaaag
      181 gacgaggagc gcgcagtgaa ggacgagctg ctgggcgaga agcctgagat caagcagaag
      241 tgggcatccc ggctgttggc caagctgcgc aaagacatcc ggcccgagtt ccgcgaggac
      301 tttgtgctaa ccatcacggg caagaagccc ccctgctgcg tgctttccaa ccccgaccag
      361 aagggcaaga tccggcggat tgactgcctg cgccaggctg acaaggtgtg gcggctggac
      421 ctggtcatgg tgattttgtt taaagggatc cctttggaaa gtactgatgg ggagcggctc
      481 tacaagtcgc cccagtgctc gaaccccggc ctgtgtgtcc agccacatca cattggagtc
      541 acaatcaaag aactggacct ttatctggct tactttgtcc acactccgga atccggacaa
      601 tcagatagtt caaaccagca aggagatgcg gacatcaaac cactgcccaa cgggcactta
      661 agtttccagg actgctttgt gacgtctggg gtctggaatg tgacagagct ggtgagagta
      721 tcacagactc cagttgcgac tgcatcaggg cccaacttct cactggcgga cctggagagc
      781 cccagctact acaacataaa tcaagtgacc ctgggaaggc ggtccatcac ctcccctcct
      841 tccaccagca gcaccaagcg ccccaagtcc atcgacgaca gtgagatgga gagtccagta
      901 gatgatgtgt tctatcctgg gacaggccgc tctccggccg ctggcagcag ccagtctagc
      961 ggatggccca atgacgtgga tgcagggagc ccccgggcca cggcatcagc gctgcacttc
     1021 ccttccacgt ccatcatcca gcagtcgagc ccgtatttca cacacccaac catccgctac
     1081 caccaccacc atgggcagga ctcgctgaag gagtttgtgc agtttgtgtg ctctgacggc
     1141 tcgggtcagg ccaccggaca gcccaacggt agcggccagg gcaaagtccc ggggtcattt
     1201 ttgctaccgc cgccgcctcc agtggccaga cctgtgcccc ttcctatgcc tgattccaaa
     1261 accaccagca cggccccaga cggcgccgcc ttgactcctc catcaccttc attcacaacg
     1321 acaggcgcct cctctgccaa ccggtttgtc agcatcggat cccgggacgg caactttctg
     1381 aacatcccac agcagtctca gtcctggttc ctctgattaa gatcaacaaa gaaacaacta
     1441 aatttaaaaa aggaaaaaaa aaaaagaggt tcctcaaaat gggggagaag aaattttgag
     1501 acatggaaat atcccccagc ccagccccac cgaaaagcaa aacttagacg tcgtcagcca
     1561 ctcagccctt ccctcctcca gccctggtac ccccgcgttc caaagccgcc cagttctggg
     1621 agtcctcgga aaggggtctc agcggagctg tacaccagca gccaagcaga aagaagcatg
     1681 ctaagcggac tctgctaagc agaggacaaa cagaaagaaa ggatgcaaca agactgcctt
     1741 cctcctactc agcctggcct tcccaggaag gagcagccag ctcactggcc ggccaccctg
     1801 gcagttcctg gggaccccac cctccgctgg acttaagctt cccaaggagc ccagaggaac
     1861 agctcgtaac aatagtagac attcgagctc ccctccggac ttcgtctctt tccctcaccc
     1921 caccccccac tccccaacct ctcttgccct tcctgggagc ctctgctcag gcttctggcc
     1981 ccagctgctc tggaccaggc tagggcgttc ccgggtaggt aagggatgct tcttagagct
     2041 aactgaattc agcctgagtc cagaatagag ggtgttctgc caccccattt tgaaccgccc
     2101 gggcagttct cgggctggac accctctgta cagcacacag gaaagtcaga atgctctgga
     2161 ctggacgccc agcccagcat aggcaccttc ttccacgccc gcaggacgca agggctgctg
     2221 ccctctgcca cgcccctagt gaagggagtc ggccccaggt cgccccgcac cccagccctg
     2281 catgcaggtg cccttgctcc gcccctctgc cctgcccctg cccttgctgc acacagccct
     2341 ccaggggccg gctgagagtg gagcagaaag ggcccccgga gctgagcgag gaggacaagg
     2401 cagcctccac ctgcgctagg taggcgtcct gctctcgaga ctttgagttc ctgtggaggg
     2461 tggggggtgt catcctttta aaagagtgtt ctggagcttt ctggtcccct tcccttccca
     2521 ccacgccccg tggccacttc tccctagatt ttagctgtaa tgtctttcct ctttatttag
     2581 gggtggggca gtcattgttt gggtctttcg ctgtttgcag ttgggaactc ctctgtttga
     2641 gcaacttggg aacaatttcg taacacacca caggaagcag ttctcctccc agccccctcc
     2701 tccagtaagg gatggcggag gcttgtccag agggtcttga gaagccccct gggagggagg
     2761 ggaacgcgag cacgccagac cccctgcagg gtgactgggc ccctctggct tgtctttctg
     2821 tgccttagtc ctccttccct gcatctcccc tcccaggccc gttctcaagg ccatgcgcct
     2881 ctctccggta tgaaaacaca aagcacaggt caggatcttc cgaaagtcgg tccaacattg
     2941 caccacgtgg gggtgggttg tgggctttat ttattgagaa tctagttggt gaggctgcgg
     3001 ccctgaacag gagaatggcc gctagcaggg agctcggggc tgcgaggcgg ctgtccctgc
     3061 gttctgtgtg gaagttgagc gaagccctcc cctgcggcgt cgagggccag gccggggtgg
     3121 acgtccagcg gggggaggcc tccctgtgcg aggggaggga gccccagggt ggggggccgg
     3181 gcgggccggg cgtgacgcgc ggtcaaagtg caatgatttt tcagttcggt tggctaaaca
     3241 gggtcagagc taagagctag cgaaagaggg tccctgcccg gccgcgtgcg cctcttcggt
     3301 cggtcgagga cagtcgggct gtgggcgctg tgggattgcg gggcacacgc cccgggcgag
     3361 gtcagaagct gcaggccagc tgggccgggg cccaaggtgc ccgagagagg taccctcggg
     3421 aggactgggg tgggggtggt gctcctttcc caagtggtgg tgtgaggggc aggcaggcag
     3481 ggacgatgag ggcgacagat gtggggacgt gtttaggaga ggaaacaacc cacaaaaaaa
     3541 tataggggaa attaaccttt ttttttttat tgaaccaagt gcaatgcatc agagagtttt
     3601 cctatctttg tatgttaaga gattaagaaa aaaaattcta tttttgttgt aatgtcctcg
     3661 cggctctggg gacgctaaaa gaactgggcc tgccctgccc ttagcgggga tgaaagctga
     3721 gtgttttcct tttttttgtt tttttttttg tttgttttcc tttttgatag ttttctgggt
     3781 ttttgtttgt tggtttggtt ggttggtttt ttttttgttt tttttttttt gttttgcctt
     3841 ttttcctgcg ttgacgagga taatctgggg tttaaatttg tttcgtcgtt cggtttcggt
     3901 gggagggctg aagaaaacgt ttaaaaacac ctcgacatta aaaaaaaaaa accaacacaa
     3961 gatcaaaagg aaaaggacga gagaaaatta tttttaagat aattaaacat aaaaaccctg
     4021 gtgcttatta cattataaag tacgttttta aaaacccaca aactattata catacgttta
     4081 tgaatcaatt aaatactctg cacttgttag gaacacgcat atcccttctt tgttgagttt
     4141 aacggaacgg gacagcggcg tgcgccccgc ggctgggctg ctctggccgc gggtctcccc
     4201 aggcgctccc cccttcctcg ccccctcccc ctccgggcac tggggcgcgg cgggggtccc
     4261 cggccccctc ccccgcagag gtcaatgcca acgaacaaac gtcccttttc cctccctccc
     4321 cactccgagc gcccttcttt gagccagacg ccaacttgac cctcaccagc attaatcagg
     4381 agcgcgctca gcaagttggt agtttcctcc ccgacccctt cccacgtaac accactattc
     4441 cccctccctc cgggaggttc gatgctccag gacaggtcca gccacgctga caggtcgatt
     4501 tgcccaggcc cgcgcccgca cgcacgcacg cacatagccc ggcacacacc cccaccctac
     4561 cccgcaacca gccctgtcga ctgccttaca cacccgcccc cgcgctggcc ggccgaccta
     4621 gtgccttgtt ctcacccccg tgctggcgga gcggacgccg cgctctgggt cccagagggg
     4681 ccgggtggct cagacgaccc accgtttccc ccaacccgac cgtactgaac ggacccccca
     4741 cgagagaaaa aaaaaaggag caataaagtt atgagaactt tgtcccccaa tcgagagccc
     4801 taggggcacc ccagccccac ctctgctccc tcccacccca tccaccctcg gggcgtccca
     4861 ctctgcgcaa gccagctggg gccaacccct gctttggccc ctcttgggag atccgtgcgc
     4921 cccatcagca ccagcatcgc ggaccgcaaa ggccgcccgt cccgtcaaac aagtttcttc
     4981 ttaggctaag aaacgcagta tatacgagta tctctatata tatatagtac taatggattt
     5041 ggtgtgcttc ccccttagcg tcccttccct ctgctcctct ttcagcctgg tctcccttct
     5101 ctgccctcca attcccctcc cccgtctctg cactgagata cataagaaac aagggtagtt
     5161 tactgtctgt tttgttttct gggttttcag tgtcctagcg gaatgcaagt aggcagccag
     5221 cccgtctgtc ccctctccgc ccgccccgcc ccgccccacc ccagtcactg cgcttctgtt
     5281 ataccatctt tgcctgactc tcttcggctt ctccattgaa tggctaatgt gtatgtgaaa
     5341 taaagaaata aagaaaaaca aatgcaa
//
