LOCUS       NM_001297601            5216 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus nuclear factor I/X (Nfix), transcript variant 4, mRNA.
ACCESSION   NM_001297601 XM_006530765
VERSION     NM_001297601.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Caria,C.A., Faa,V., Porcu,S., Marongiu,M.F., Poddie,D., Perseu,L.,
            Meloni,A., Vaccargiu,S. and Ristaldi,M.S.
  TITLE     Post-GWAS Validation of Target Genes Associated with HbF and HbA2
            Levels
  JOURNAL   Cells 13 (14), 1185 (2024)
   PUBMED   39056767
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Kooblall,K.G., Stevenson,M., Heilig,R., Stewart,M., Wright,B.,
            Lockstone,H., Buck,D., Fischer,R., Wells,S., Lines,K.E., Teboul,L.,
            Hennekam,R.C. and Thakker,R.V.
  TITLE     Identification of cellular retinoic acid binding protein 2 (CRABP2)
            as downstream target of nuclear factor I/X (NFIX): implications for
            skeletal dysplasia syndromes
  JOURNAL   JBMR Plus 8 (7), ziae060 (2024)
   PUBMED   38827116
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5216)
  AUTHORS   Angelini,G., Capra,E., Rossi,F., Mura,G., Saclier,M., Taglietti,V.,
            Rovetta,G., Epis,R., Careccia,G., Bonfanti,C. and Messina,G.
  TITLE     MEK-inhibitors decrease Nfix in muscular dystrophy but induce
            unexpected calcifications, partially rescued with Cyanidin diet
  JOURNAL   iScience 27 (1), 108696 (2023)
   PUBMED   38205246
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5216)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5216)
  AUTHORS   Pan,D., Zhong,J., Zhang,J., Dong,H., Zhao,D., Zhang,H. and Yao,B.
  TITLE     Function and regulation of nuclear factor 1 X-type on chondrocyte
            proliferation and differentiation
  JOURNAL   Gene 881, 147620 (2023)
   PUBMED   37433356
  REMARK    GeneRIF: Function and regulation of nuclear factor 1 X-type on
            chondrocyte proliferation and differentiation.
REFERENCE   6  (bases 1 to 5216)
  AUTHORS   Fletcher,C.F., Lutz,C.M., O'Sullivan,T.N., Shaughnessy,J.D. Jr.,
            Hawkes,R., Frankel,W.N., Copeland,N.G. and Jenkins,N.A.
  TITLE     Absence epilepsy in tottering mutant mice is associated with
            calcium channel defects
  JOURNAL   Cell 87 (4), 607-617 (1996)
   PUBMED   8929530
REFERENCE   7  (bases 1 to 5216)
  AUTHORS   Kulkarni,S. and Gronostajski,R.M.
  TITLE     Altered expression of the developmentally regulated NFI gene family
            during phorbol ester-induced differentiation of human leukemic
            cells
  JOURNAL   Cell Growth Differ 7 (4), 501-510 (1996)
   PUBMED   9052991
REFERENCE   8  (bases 1 to 5216)
  AUTHORS   Qian,F., Kruse,U., Lichter,P. and Sippel,A.E.
  TITLE     Chromosomal localization of the four genes (NFIA, B, C, and X) for
            the human transcription factor nuclear factor I by FISH
  JOURNAL   Genomics 28 (1), 66-73 (1995)
   PUBMED   7590749
REFERENCE   9  (bases 1 to 5216)
  AUTHORS   Nebl,G. and Cato,A.C.
  TITLE     NFI/X proteins: a class of NFI family of transcription factors with
            positive and negative regulatory domains
  JOURNAL   Cell Mol Biol Res 41 (2), 85-95 (1995)
   PUBMED   8581067
REFERENCE   10 (bases 1 to 5216)
  AUTHORS   Scherthan,H., Seisenberger,C., Greulich,K. and Winnacker,E.L.
  TITLE     Mapping of the murine nuclear factor I/X gene (Nfix) to mouse
            chromosome 8 C1-2 by FISH
  JOURNAL   Genomics 22 (1), 247-249 (1994)
   PUBMED   7959784
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155163.4 and AC145556.4.
            
            On Jun 26, 2019 this sequence version replaced NM_001297601.1.
            
            Transcript Variant: This variant (4) lacks an alternate in-frame
            exon and another alternate exon compared to variant 3, that causes
            a frameshift. The resulting isoform (4) has a shorter and distinct
            C-terminus and lacks an alternate internal segment compared to
            isoform 3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158568.1, SRR7345562.1143333.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AC155163.4         118014-118070       c
            58-589              AC155163.4         116444-116975       c
            590-652             AC155163.4         72565-72627         c
            653-727             AC155163.4         72265-72339         c
            728-848             AC155163.4         71784-71904         c
            849-982             AC155163.4         71129-71262         c
            983-1158            AC155163.4         66303-66478         c
            1159-1250           AC155163.4         58426-58517         c
            1251-1893           AC155163.4         52695-53337         c
            1894-5216           AC145556.4         175078-178400       c
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.02 cM"
     gene            1..5216
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="nuclear factor I/X"
                     /db_xref="GeneID:18032"
                     /db_xref="MGI:MGI:97311"
     exon            1..57
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1230
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="isoform 4 is encoded by transcript variant 4;
                     nuclear factor 1 X-type; nuclear factor 1/X; TGGCA-binding
                     protein; CCAAT-box-binding transcription factor"
                     /codon_start=1
                     /product="nuclear factor 1 X-type isoform 4"
                     /protein_id="NP_001284530.1"
                     /db_xref="CCDS:CCDS80907.1"
                     /db_xref="GeneID:18032"
                     /db_xref="MGI:MGI:97311"
                     /translation="MDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDE
                     ERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQ
                     KGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQCSNPGLCVQPHHI
                     GVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHLSFQDCFVTSGVWNVTE
                     LVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSTKRPKSIDDSE
                     MESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGSPRATASALHFPSTSIIQQSSPYF
                     THPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQHSQRQAPPLPTGLSASDPGTATF"
     exon            58..589
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            590..652
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            653..727
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            728..848
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            849..982
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1158
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1250
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..5216
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1385
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="major polyA site"
     regulatory      5196..5201
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="hexamer: AATAAA"
     regulatory      5200..5205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="hexamer: AAGAAA"
     polyA_site      5211
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
     polyA_site      5216
                     /gene="Nfix"
                     /gene_synonym="CTF; NF-I/X; NF1-X; NFI-X"
                     /note="major polyA site"
ORIGIN      
        1 gttgagccca gaatctcaga atcaggcgtt ggctttgccg ggtgcttcag atcaatggat
       61 gagttccacc cgtttatcga ggcgctgctg cctcacgtcc gagccttctc ctacacctgg
      121 ttcaacctgc aggcgcggaa gcgcaagtac ttcaagaagc acgagaagcg gatgtcaaag
      181 gacgaggagc gcgcagtgaa ggacgagctg ctgggcgaga agcctgagat caagcagaag
      241 tgggcatccc ggctgttggc caagctgcgc aaagacatcc ggcccgagtt ccgcgaggac
      301 tttgtgctaa ccatcacggg caagaagccc ccctgctgcg tgctttccaa ccccgaccag
      361 aagggcaaga tccggcggat tgactgcctg cgccaggctg acaaggtgtg gcggctggac
      421 ctggtcatgg tgattttgtt taaagggatc cctttggaaa gtactgatgg ggagcggctc
      481 tacaagtcgc cccagtgctc gaaccccggc ctgtgtgtcc agccacatca cattggagtc
      541 acaatcaaag aactggacct ttatctggct tactttgtcc acactccgga atccggacaa
      601 tcagatagtt caaaccagca aggagatgcg gacatcaaac cactgcccaa cgggcactta
      661 agtttccagg actgctttgt gacgtctggg gtctggaatg tgacagagct ggtgagagta
      721 tcacagactc cagttgcgac tgcatcaggg cccaacttct cactggcgga cctggagagc
      781 cccagctact acaacataaa tcaagtgacc ctgggaaggc ggtccatcac ctcccctcct
      841 tccaccagca ccaagcgccc caagtccatc gacgacagtg agatggagag tccagtagat
      901 gatgtgttct atcctgggac aggccgctct ccggccgctg gcagcagcca gtctagcgga
      961 tggcccaatg acgtggatgc agggagcccc cgggccacgg catcagcgct gcacttccct
     1021 tccacgtcca tcatccagca gtcgagcccg tatttcacac acccaaccat ccgctaccac
     1081 caccaccatg ggcaggactc gctgaaggag tttgtgcagt ttgtgtgctc tgacggctcg
     1141 ggtcaggcca ccggacagca ttcacaacga caggcgcctc ctctgccaac cggtttgtca
     1201 gcatcggatc ccgggacggc aactttctga acatcccaca gcagtctcag tcctggttcc
     1261 tctgattaag atcaacaaag aaacaactaa atttaaaaaa ggaaaaaaaa aaaagaggtt
     1321 cctcaaaatg ggggagaaga aattttgaga catggaaata tcccccagcc cagccccacc
     1381 gaaaagcaaa acttagacgt cgtcagccac tcagcccttc cctcctccag ccctggtacc
     1441 cccgcgttcc aaagccgccc agttctggga gtcctcggaa aggggtctca gcggagctgt
     1501 acaccagcag ccaagcagaa agaagcatgc taagcggact ctgctaagca gaggacaaac
     1561 agaaagaaag gatgcaacaa gactgccttc ctcctactca gcctggcctt cccaggaagg
     1621 agcagccagc tcactggccg gccaccctgg cagttcctgg ggaccccacc ctccgctgga
     1681 cttaagcttc ccaaggagcc cagaggaaca gctcgtaaca atagtagaca ttcgagctcc
     1741 cctccggact tcgtctcttt ccctcacccc accccccact ccccaacctc tcttgccctt
     1801 cctgggagcc tctgctcagg cttctggccc cagctgctct ggaccaggct agggcgttcc
     1861 cgggtaggta agggatgctt cttagagcta actgaattca gcctgagtcc agaatagagg
     1921 gtgttctgcc accccatttt gaaccgcccg ggcagttctc gggctggaca ccctctgtac
     1981 agcacacagg aaagtcagaa tgctctggac tggacgccca gcccagcata ggcaccttct
     2041 tccacgcccg caggacgcaa gggctgctgc cctctgccac gcccctagtg aagggagtcg
     2101 gccccaggtc gccccgcacc ccagccctgc atgcaggtgc ccttgctccg cccctctgcc
     2161 ctgcccctgc ccttgctgca cacagccctc caggggccgg ctgagagtgg agcagaaagg
     2221 gcccccggag ctgagcgagg aggacaaggc agcctccacc tgcgctaggt aggcgtcctg
     2281 ctctcgagac tttgagttcc tgtggagggt ggggggtgtc atccttttaa aagagtgttc
     2341 tggagctttc tggtcccctt cccttcccac cacgccccgt ggccacttct ccctagattt
     2401 tagctgtaat gtctttcctc tttatttagg ggtggggcag tcattgtttg ggtctttcgc
     2461 tgtttgcagt tgggaactcc tctgtttgag caacttggga acaatttcgt aacacaccac
     2521 aggaagcagt tctcctccca gccccctcct ccagtaaggg atggcggagg cttgtccaga
     2581 gggtcttgag aagccccctg ggagggaggg gaacgcgagc acgccagacc ccctgcaggg
     2641 tgactgggcc cctctggctt gtctttctgt gccttagtcc tccttccctg catctcccct
     2701 cccaggcccg ttctcaaggc catgcgcctc tctccggtat gaaaacacaa agcacaggtc
     2761 aggatcttcc gaaagtcggt ccaacattgc accacgtggg ggtgggttgt gggctttatt
     2821 tattgagaat ctagttggtg aggctgcggc cctgaacagg agaatggccg ctagcaggga
     2881 gctcggggct gcgaggcggc tgtccctgcg ttctgtgtgg aagttgagcg aagccctccc
     2941 ctgcggcgtc gagggccagg ccggggtgga cgtccagcgg ggggaggcct ccctgtgcga
     3001 ggggagggag ccccagggtg gggggccggg cgggccgggc gtgacgcgcg gtcaaagtgc
     3061 aatgattttt cagttcggtt ggctaaacag ggtcagagct aagagctagc gaaagagggt
     3121 ccctgcccgg ccgcgtgcgc ctcttcggtc ggtcgaggac agtcgggctg tgggcgctgt
     3181 gggattgcgg ggcacacgcc ccgggcgagg tcagaagctg caggccagct gggccggggc
     3241 ccaaggtgcc cgagagaggt accctcggga ggactggggt gggggtggtg ctcctttccc
     3301 aagtggtggt gtgaggggca ggcaggcagg gacgatgagg gcgacagatg tggggacgtg
     3361 tttaggagag gaaacaaccc acaaaaaaat ataggggaaa ttaacctttt tttttttatt
     3421 gaaccaagtg caatgcatca gagagttttc ctatctttgt atgttaagag attaagaaaa
     3481 aaaattctat ttttgttgta atgtcctcgc ggctctgggg acgctaaaag aactgggcct
     3541 gccctgccct tagcggggat gaaagctgag tgttttcctt ttttttgttt ttttttttgt
     3601 ttgttttcct ttttgatagt tttctgggtt tttgtttgtt ggtttggttg gttggttttt
     3661 tttttgtttt tttttttttg ttttgccttt tttcctgcgt tgacgaggat aatctggggt
     3721 ttaaatttgt ttcgtcgttc ggtttcggtg ggagggctga agaaaacgtt taaaaacacc
     3781 tcgacattaa aaaaaaaaaa ccaacacaag atcaaaagga aaaggacgag agaaaattat
     3841 ttttaagata attaaacata aaaaccctgg tgcttattac attataaagt acgtttttaa
     3901 aaacccacaa actattatac atacgtttat gaatcaatta aatactctgc acttgttagg
     3961 aacacgcata tcccttcttt gttgagttta acggaacggg acagcggcgt gcgccccgcg
     4021 gctgggctgc tctggccgcg ggtctcccca ggcgctcccc ccttcctcgc cccctccccc
     4081 tccgggcact ggggcgcggc gggggtcccc ggccccctcc cccgcagagg tcaatgccaa
     4141 cgaacaaacg tcccttttcc ctccctcccc actccgagcg cccttctttg agccagacgc
     4201 caacttgacc ctcaccagca ttaatcagga gcgcgctcag caagttggta gtttcctccc
     4261 cgaccccttc ccacgtaaca ccactattcc ccctccctcc gggaggttcg atgctccagg
     4321 acaggtccag ccacgctgac aggtcgattt gcccaggccc gcgcccgcac gcacgcacgc
     4381 acatagcccg gcacacaccc ccaccctacc ccgcaaccag ccctgtcgac tgccttacac
     4441 acccgccccc gcgctggccg gccgacctag tgccttgttc tcacccccgt gctggcggag
     4501 cggacgccgc gctctgggtc ccagaggggc cgggtggctc agacgaccca ccgtttcccc
     4561 caacccgacc gtactgaacg gaccccccac gagagaaaaa aaaaaggagc aataaagtta
     4621 tgagaacttt gtcccccaat cgagagccct aggggcaccc cagccccacc tctgctccct
     4681 cccaccccat ccaccctcgg ggcgtcccac tctgcgcaag ccagctgggg ccaacccctg
     4741 ctttggcccc tcttgggaga tccgtgcgcc ccatcagcac cagcatcgcg gaccgcaaag
     4801 gccgcccgtc ccgtcaaaca agtttcttct taggctaaga aacgcagtat atacgagtat
     4861 ctctatatat atatagtact aatggatttg gtgtgcttcc cccttagcgt cccttccctc
     4921 tgctcctctt tcagcctggt ctcccttctc tgccctccaa ttcccctccc ccgtctctgc
     4981 actgagatac ataagaaaca agggtagttt actgtctgtt ttgttttctg ggttttcagt
     5041 gtcctagcgg aatgcaagta ggcagccagc ccgtctgtcc cctctccgcc cgccccgccc
     5101 cgccccaccc cagtcactgc gcttctgtta taccatcttt gcctgactct cttcggcttc
     5161 tccattgaat ggctaatgtg tatgtgaaat aaagaaataa agaaaaacaa atgcaa
//
