LOCUS       NM_182716               9795 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus neurofascin (Nfasc), transcript variant 1, mRNA.
ACCESSION   NM_182716
VERSION     NM_182716.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Bekku,Y., Zotter,B., You,C., Piehler,J., Leonard,W.J. and
            Salzer,J.L.
  TITLE     Glia trigger endocytic clearance of axonal proteins to promote
            rodent myelination
  JOURNAL   Dev Cell 59 (5), 627-644 (2024)
   PUBMED   38309265
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 9795)
  AUTHORS   Hatayama,M., Katayama,K.I., Kawahara,Y., Matsunaga,H.,
            Takashima,N., Iwayama,Y., Matsumoto,Y., Nishi,A., Yoshikawa,T. and
            Aruga,J.
  TITLE     SLITRK1-mediated noradrenergic projection suppression in the
            neonatal prefrontal cortex
  JOURNAL   Commun Biol 5 (1), 935 (2022)
   PUBMED   36085162
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9795)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9795)
  AUTHORS   Ghosh,A., Malavasi,E.L., Sherman,D.L. and Brophy,P.J.
  TITLE     Neurofascin and Kv7.3 are delivered to somatic and axon terminal
            surface membranes en route to the axon initial segment
  JOURNAL   Elife 9, e60619 (2020)
   PUBMED   32903174
  REMARK    GeneRIF: Neurofascin and Kv7.3 are delivered to somatic and axon
            terminal surface membranes en route to the axon initial segment.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9795)
  AUTHORS   Hortsch,M.
  TITLE     Structural and functional evolution of the L1 family: are four
            adhesion molecules better than one?
  JOURNAL   Mol Cell Neurosci 15 (1), 1-10 (2000)
   PUBMED   10662501
  REMARK    Review article
REFERENCE   7  (bases 1 to 9795)
  AUTHORS   Yamazaki,K., Oki,T. and Tanaka,I.
  TITLE     Cloning of 5'-flanking region and a polymorphic CTT trinucleotide
            repeat within 5'-untranslated region of mouse R-type calcium
            channel alpha1-subunit (Cchra1) gene, and its genetic mapping
  JOURNAL   Gene 214 (1-2), 199-204 (1998)
   PUBMED   9651528
REFERENCE   8  (bases 1 to 9795)
  AUTHORS   Burmeister,M., Ren,Q., Makris,G.J., Samson,D. and Bennett,V.
  TITLE     Genes for the neuronal immunoglobulin domain cell adhesion
            molecules neurofascin and Nr-CAM map to mouse chromosomes 1 and 12
            and homologous human chromosomes
  JOURNAL   Mamm Genome 7 (7), 558-559 (1996)
   PUBMED   8672144
REFERENCE   9  (bases 1 to 9795)
  AUTHORS   Edwards,R.A., Herrera-Sosa,H., Otto,J. and Bryan,J.
  TITLE     Cloning and expression of a murine fascin homolog from mouse brain
  JOURNAL   J Biol Chem 270 (18), 10764-10770 (1995)
   PUBMED   7738015
REFERENCE   10 (bases 1 to 9795)
  AUTHORS   Moscoso,L.M. and Sanes,J.R.
  TITLE     Expression of four immunoglobulin superfamily adhesion molecules
            (L1, Nr-CAM/Bravo, neurofascin/ABGP, and N-CAM) in the developing
            mouse spinal cord
  JOURNAL   J Comp Neurol 352 (3), 321-334 (1995)
   PUBMED   7706555
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124095.7, AC116691.12,
            AC159974.7 and AI847023.1.
            
            On May 21, 2009 this sequence version replaced NM_182716.3.
            
            Summary: This gene encodes an L1 family immunoglobulin cell
            adhesion molecule with multiple IGcam and fibronectin domains. The
            protein functions in neurite outgrowth, neurite fasciculation, and
            organization of the axon initial segment (AIS) and nodes of Ranvier
            on axons during early development. Both the AIS and nodes of
            Ranvier contain high densities of voltage-gated Na+ (Nav) channels
            which are clustered by interactions with cytoskeletal and
            scaffolding proteins including this protein, gliomedin, ankyrin 3
            (ankyrin-G), and betaIV spectrin. This protein links the AIS
            extracellular matrix to the intracellular cytoskeleton. This gene
            undergoes extensive alternative splicing, and the full-length
            nature of some variants has not been determined. [provided by
            RefSeq, May 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). This isoform lacks
            the third fibronectin type 3 (FNIII) repeat and includes the
            mucin-like domain. Isoform 1 is also known as NF186, as described
            by Davis et al. (PMID: 8947556).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ543322.1, SRR7974084.21444.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC124095.7         182045-182171
            128-236             AC116691.12        61457-61565
            237-417             AC116691.12        84186-84366
            418-435             AC116691.12        89163-89180
            436-541             AC116691.12        89953-90058
            542-738             AC116691.12        92224-92420
            739-861             AC116691.12        92754-92876
            862-1032            AC116691.12        95189-95359
            1033-1144           AC116691.12        105421-105532
            1145-1329           AC116691.12        105935-106119
            1330-1461           AC116691.12        107524-107655
            1462-1605           AC116691.12        115371-115514
            1606-1651           AC116691.12        116158-116203
            1652-1717           AC159974.7         158836-158901       c
            1718-1884           AC159974.7         158372-158538       c
            1885-2032           AC159974.7         157818-157965       c
            2033-2157           AC159974.7         156444-156568       c
            2158-2202           AC159974.7         155064-155108       c
            2203-2304           AC159974.7         153545-153646       c
            2305-2502           AC159974.7         153102-153299       c
            2503-2573           AC159974.7         151859-151929       c
            2574-2796           AC159974.7         150032-150254       c
            2797-2919           AC159974.7         144587-144709       c
            2920-3093           AC159974.7         143545-143718       c
            3094-3108           AC159974.7         141412-141426       c
            3109-3345           AC159974.7         136135-136371       c
            3346-3462           AC159974.7         132425-132541       c
            3463-3615           AC159974.7         131056-131208       c
            3616-3747           AC159974.7         124347-124478       c
            3748-3817           AC159974.7         121831-121900       c
            3818-9495           AC159974.7         113035-118712       c
            9496-9795           AI847023.1         1-300               c
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.42 cM"
     gene            1..9795
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /note="neurofascin"
                     /db_xref="GeneID:269116"
                     /db_xref="MGI:MGI:104753"
     exon            1..127
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            128..236
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            237..417
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..4049
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="neurofascin isoform 1 precursor"
                     /protein_id="NP_874385.1"
                     /db_xref="CCDS:CCDS35707.1"
                     /db_xref="GeneID:269116"
                     /db_xref="MGI:MGI:104753"
                     /translation="MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTIT
                     KQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVID
                     FRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTL
                     QCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFH
                     FTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTP
                     DIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVK
                     AAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDT
                     IIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRL
                     DCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILG
                     KAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI
                     GNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALP
                     KGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFP
                     GSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTR
                     KNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYE
                     IRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILI
                     GYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATE
                     ESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVA
                     TTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWK
                     HNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISST
                     VITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKD
                     VPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNE
                     DGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA"
     exon            418..435
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            436..541
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            542..738
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            739..861
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1032
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1144
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1329
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1461
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1605
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1717
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1884
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2032
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2157
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2202
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2304
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2502
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2573
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2796
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2919
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3093
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3108
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3345
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3462
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3615
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3616..3747
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3748..3817
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3818..9779
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5220
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
     regulatory      9758..9763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /note="hexamer: AATAAA"
     polyA_site      9779
                     /gene="Nfasc"
                     /gene_synonym="D430023G06Rik; mKIAA0756; NF"
                     /note="major polyA site"
ORIGIN      
        1 acgggctagt ctctgcccta atgcggcggc tggcagcgag aggtgctgca ggggacgcga
       61 gggaagtggc ggcgccggga gcggacatct gggactgcgc ccggggctgg agcccgagct
      121 cctcgaggtt gactgactta tgtgcgcttt ggcagcctgg agttttcttg ccctctgccg
      181 acgggagcag agcctctgct ggtgttggga aaagcaggcc gaatgaggca gagaagctga
      241 gcactgtctg gaaagcccac atagagcggc acaaggcatc gggagcccgt gggctgggca
      301 gcggggagca gggcctggtg ctgaggatgg ccaggcagca ggcgccaccc tgggtccaca
      361 tagccctcat cctcttcctc ctcagcctcg gaggggccat cgagattccg atggacccaa
      421 gcattcagaa tgagctgacc caacccccaa ctatcaccaa gcagtccgtg aaggaccaca
      481 tcgtggaccc tcgagataac atcctgattg aatgtgaagc taaaggcaac cccgccccca
      541 gttttcactg gactcgcaac agcagattct tcaacattgc caaggaccca cgggtgtcca
      601 tgaggaggag atctgggacc ttggtgatcg acttccgcag tggtgggcgg cctgaggaat
      661 acgaagggga gtaccagtgc tttgcccgga acaaatttgg cactgcactt agcaaccgca
      721 tccgcctgca ggtgtccaaa tctcccctgt ggcccaagga aaacctagac cccgtcgtgg
      781 ttcaagaggg tgcccccttg acactacagt gcaacccccc acccggcctc ccgtcccccg
      841 tcatcttctg gatgagcagc tccatggagc ccatcaccca ggacaagcgt gtctcccagg
      901 gtcacaacgg ggacctgtac ttctccaacg tcatgctgca ggacatgcag accgactaca
      961 gctgcaacgc gcgctttcac ttcacccaca ccattcagca gaagaacccc ttcaccctca
     1021 aggtcctcac cacccgagga gttgcagaaa gaacgcccag cttcatgtat ccccagggca
     1081 catcgagcag tcagatggtt ctccgtggca tggacctgct gctggaatgc attgcctctg
     1141 gcgtcccaac accagacatt gcatggtaca agaaaggtgg ggacctccca tctaacaagg
     1201 ccaagttcga gaactttaat aaggctctgc gcatcaccaa tgtctctgaa gaggactctg
     1261 gggagtattt ctgcctggcc tccaacaaga tgggcagcat ccggcacacg atctcggtga
     1321 gagtaaaggc tgctccatac tggctggatg agcccaagaa cctgatcctg gctcctgggg
     1381 aagatgggag gctggtatgc cgagccaatg ggaacccgaa gccgaccgtg cagtggatgg
     1441 tgaatggaga gcctttacaa tcggcaccac ccaatcccaa ccgtgaggta gctggagaca
     1501 ctatcatctt ccgggatact cagatcagca gcagggcagt gtaccaatgt aatacatcca
     1561 atgaacatgg ctacctgctg gccaatgcct tcgtcagcgt gttagatgta ccccctcgga
     1621 tgctgtctgc ccgcaaccag ctcatcaggg tgatccttta taaccggaca cggctggact
     1681 gtccgttctt tgggtctccc atcccaacac tccgatggtt taagaatggg caaggaagca
     1741 acctggatgg cggtaactac cacgtctacg aaaacggcag tctagaaatc aagatgattc
     1801 gcaaagagga ccaaggcatc tacacctgtg tggccaccaa catcctgggc aaagccgaaa
     1861 atcaagtccg cctggaggtc aaagacccca ccaggatcta caggatgccc gaggaccagg
     1921 tggccaagag gggcaccacg gtgcagctgg agtgccgcgt gaaacatgac ccctccttga
     1981 agctcacagt ctcctggctg aaggacgatg agccactcta cattggaaac aggatgaaga
     2041 aggaagatga ctccctgacg atcttcggag tggcagagcg ggaccagggc agttacacgt
     2101 gtatggccag caccgagctg gaccaggacc tggcaaaggc ctacctcact gttctagctg
     2161 atcaggccac tccaactaac cgtttggctg ccctacccaa agggcgacca gaccgaccca
     2221 gggacctgga gctcactgac ctggctgaga ggagtgtgag gctgacctgg atcccagggg
     2281 atgacaacaa cagccctatc acagactacg tcgttcagtt tgaagaggac cagttccaac
     2341 caggggtgtg gcatgaccac tccaggttcc caggcagcgt caactcagcc gtcctccatc
     2401 tgtccccata tgtcaactac caattcagag tcatcgctgt caacgaggtt gggagcagcc
     2461 accccagcct tccatccgag cggtaccgaa ccagtggggc accccctgaa tctaatccca
     2521 gtgatgtgaa gggcgaaggg acaagaaaga acaatatgga gatcacgtgg acgcctatga
     2581 atgctacctc tgcctttggc cccaacctac gctacattgt caagtggcga cggagagaga
     2641 cccgagagac ttggaacaat gtcacagtgt ggggctctcg ctacgtggtg gggcagacgc
     2701 ctgtctacgt tccctatgag atccgagtcc aggctgaaaa tgactttggg aaaggccccg
     2761 agcctgacac catcattggg tactccggag aagatttacc cagtgccccc aggcggttca
     2821 gagtccgaca gcccaacctg gagaccatca acctggagtg ggaccaccca gagcacccca
     2881 acggaatcct gattggatac atcctcagat acgtgccctt taatggaacc aaactgggaa
     2941 agcagatggt ggaaaacttc tctcccaatc agaccaagtt ctctgtgcag agagcagacc
     3001 cagtgtcgcg ttaccgcttc tccctcagtg ccaggacaca ggtgggctct ggagaagcag
     3061 ccacagagga gtccccagca cctccaaatg aagctactcc aactgcagct cctcccacgt
     3121 tgcccccgac tactgtgggt accacaggcc ttgtgagcag tactgatgct actgcccttg
     3181 ctgccaccag tgaagccaca acagttccca tcattccaac cgtcgtacct accaccgtcg
     3241 ccaccaccat tgccacaact actacaacca ctgccgccac caccaccacc accactacgg
     3301 agagccctcc cactaccact gctgggacta agattcacga aaccgccccc gacgagcagt
     3361 ccatttggaa cgtcacagtg ctccccaaca gtaaatgggc caacatcacc tggaagcaca
     3421 atttcaggcc tggaactgac tttgtggttg agtacatcga cagcaaccat acgaaaaaaa
     3481 ctgtccctgt taaggcccag gcccagccta tacagctgac agacctcttt cccgggatga
     3541 cgtacacgtt gcgggtgtat tcccgggaca acgagggcat cagcagtacc gtcatcacct
     3601 ttatgaccag tacagcttac accaataacc aggcagacat cgccacccag ggctggttca
     3661 tcgggctcat gtgtgccatt gcccttctgg tgctgatcct tctcatcgtc tgcttcatca
     3721 agaggagtcg aggtggcaag tacccagtgc gggaaaagaa ggatgtcccc ttgggtcctg
     3781 aagaccccaa agaagaagat ggctcatttg actacagtga tgaggacaac aagcccctgc
     3841 agggcagcca gacatctctg gatggcacca tcaagcagca ggagagcgat gacagcctgg
     3901 tggactatgg cgaaggcggc gagggccagt tcaatgaaga tggctccttt attggccagt
     3961 acactgtcaa aaaggacaag gaggaaacgg agggcaatga gagctcagag gccacatcac
     4021 cagtcaatgc catctattcc cttgcctgac ggagtatccc aggcacggca ctactttgca
     4081 agtaggaggg gagaggggag atgaagcaac tgcagaccta ccataaagcc accaccacct
     4141 tcagggacaa gggtacaaca tgggggtctg ccaagctgtg agggccagtg accacccagc
     4201 tatccacaac cccctcctag tgatgccccg ttacccttgg gtgccattag atgggaaagc
     4261 tagagctatg ctcattgcag ggagccctag tcccttgccc tgtctcacgg ccaccctgag
     4321 cgtcccacca caatggccac cccgatctcg gctcacgatc attcatttct attgcttacg
     4381 atattttcat tctctctctc tctctctctc tctctctctc tctctgcttt gtgcatcttc
     4441 tccttccaag tcattgtctc cattttcttt tacttttgaa aaagaaaaaa aaaaagtccc
     4501 tggaaaaaga ataagtgggc tctccccaag gaaatgaaaa cgatgggcaa aaaaaggatt
     4561 gatccgtaac acaacatgca gaaaagctgt agtattcaag ctgccaccag gccagtgtgt
     4621 ttccgaagga tgcctttcgc catatgtctc ccccaacccc cactccccac ccatctgcct
     4681 cggccacatc catgctgagc tgggcttggc tcttttctgg aaaatgacag tgttttttgg
     4741 caggaagagg tgcgcaggac tcctttccat gccctgcttc agttgggagg gggccttacc
     4801 tcttgctcct ggttcttact ccgcccctgc caccagagag tctgtggatg agctgctcgg
     4861 atgatgagaa agggagacct acaaggactc tggcgaccag acccgaagct ggaagcaagt
     4921 cagagtgtgg tccctggata atggagcaaa cctccagaga agagaaaggg ctgggtttgg
     4981 acctcttttg tctttggcag tgaaacccag ggtggaggag agagggccac acctgctcac
     5041 ctggtcaggg tctatgctgc taccctccaa gagctggagc cctgagattc aggagtgtgg
     5101 ctggtgtgtc tgggggtaac agtgctcctc ctcctcccgt cctctccttg ctcaattgtg
     5161 tctgacattg acttgtgttt gacatggtat atgaatgggg atctgctgcc cccagaatga
     5221 gcctatctgt cctttcatta gggtacgcat acatatcggg ggaccgtgag gtgcaccgta
     5281 gtaacagtgc tgtgatgcct ctccctgcct tgaaggacac aaagctcttc gtatgcctta
     5341 tacagctcgg atctaaccct gcggttccca gatccctagc cctattctgc aagtcttgat
     5401 gcctccagtg atgtgtgtct cgggaccagg gcagcctatg caagtgtccc ctcgggacca
     5461 gaagcagccg ctccacacac gcccttaccc cccctccgcc ctaatggaga cactcgcagg
     5521 catgcagttc tcagcctgaa ggtgctgcct tcattccccc acctaaccca cttttgaaac
     5581 caaaggtacc ttgcctcagc gacacaaaga gatgggagat ctcaagccag agtgtcgccc
     5641 tgtagctgtg tctgcagcct gaggcagttg tgggagggca aggttgacca cttcgaatgg
     5701 atcgcccgcc attggcatgg cctccaactt gaggtactag ttaggggcct gggtgggacc
     5761 agggctgtgc attccagaga ctttccaaga gcatcatttg ccatacgttt aaaccgcaga
     5821 ggtattaaca atgctgtgtc acttgataag catttgggtg agcgtcaacc ccgtaggcac
     5881 acactctttg caggtaagaa atcatatccc agagatgagc tgataatgtt tgaacctgag
     5941 agtgtgtcat ggtcctttgg accaacccat ggccagcact gcttcctgga ggcctgaggg
     6001 acagctctca tggtgaaagg ggacagggta aacaggaagg ctaggggtgg gggtgtgctc
     6061 aggtcccctt ccctgttcta gtctaacact tctgggacac agctgggcat tgctgtagtg
     6121 taggtcacca ttcctgacgg gagggttagg gaaatgcaac tgaggtaaag aaccatcctt
     6181 cccagaaaac actgtggacc gggaggaagt ggtgggtttc tcaggcacca tttacacagc
     6241 accatccagc tagtaaagcc atggcaccaa cagcaggcat tcagaggaag ggactcggtt
     6301 gtcattgtag ctatccccct gccacaggca gcctggagag ggctctttgg tgttaagggt
     6361 ctgggagaga ggtgaatcct taggctgtct ttgtgcctca ttccggagtt ctgtcatgct
     6421 cagtactgtc ctctacagaa ggaatgaaaa gcccatcagc ccctccctaa ggctgttgtg
     6481 ttgtgggtgc tgagagctgt cccagccacc agcggtcaca gctctcctgg caggaacaga
     6541 ttgggggctg gagaaccaga gaaatggcag gttcccagac agagcagctc agcactgtga
     6601 ggtttcaagg aggcagcttt ttccaagaag gggagaggcc acttttcaca gagcctgcca
     6661 gagtctaccc tcaactcagc aagagaacag tattgccacc cctgcacctg gatcttaggc
     6721 agccacccca aagtggtgcc taggcctgct cgtgtttcat ctctcaggtg ggagcagggg
     6781 gacagggtgg gtcatcctgc agtgattctg agtgcttatc aaaagctctt tgagcatcta
     6841 gtctgggcca aagcttaagc catgaatacc atgtgaacaa gtgaggtcag atgtggtccc
     6901 tacccacgca gggctgatgt tactggaaac gagtaggtga gttcctggca caccgtgacc
     6961 attccgtcag aagcgttgtc ctggtttgcc tatactgccg attgcttcaa gtcagaaggg
     7021 agaggtttcc aaaggagaac ttgctccatt gggccagcaa gtctgggcag gacatgacgg
     7081 tcaggatgag tggattcggt aggcagcact gtagtgcttt agggtgtctg tctcccacat
     7141 ttagccccct ctgtgaatac tcctgtttgg aggaggacag gtgtacccga tcaggaccaa
     7201 ctgtccccca tcctgcctgg gatatgctaa acaggacaca tcaaggctct actcgtgtgt
     7261 agatagctga gttaatacta gagggaaagc caggctggct cctgccctta cctatttcct
     7321 ccttcccaga ggtcagtgta ggagggacca cccctcccaa caagtcccaa gagtcagcca
     7381 gtgattcagc tcacctcaca aaaggaacaa gagagcacgt tcctggtttc cctgaacaca
     7441 gtcagcattg agaagggagt aaaagcatcc tccccatccc ccatacacac acacacacac
     7501 acacacaaac acacacacac acaagcagac ccaactgtag ggaacaccct agatcataat
     7561 cttacagcat gagcatccat atcttggttc acatgagatg agctgctaaa gcctgcgggt
     7621 gaggggacct tggggaggga gaagctgttc agattgggcc ttatttttgg ttcccgtgga
     7681 cagaaggtag agaagaaatc tgaaggagag agaaactggc ctttgactct cctggcccct
     7741 cctgggagcc ccagctaaac tcggctgtcc tagagcctct ggatgggaag caaggtttcc
     7801 ttgctgttta tgaggctttt tcgtggttgc tcagaacgga tgaagcttct tgttccctcc
     7861 atggtctagg gccgaggccc agactcactt ctcagaggaa ggagagcagg ttgctagaat
     7921 gccagcccag aacccgtccc tcatctgtgt cagttcggag tggggtgtcc tccaaccaag
     7981 accagcagcc tttgcccaat gaaagcccat ggccaggatt aggtcccaac agctgggcca
     8041 cagtgctctc tttctagtga agcaaatgtc cttacatata tttcacatgg tagcctagga
     8101 atcccatgta ggcagtcccc actccaaaac aagaagtctc aagccagtga cagaaaatgt
     8161 agagtgtaac accggcagct tgggcaaaga gtccccaact cagtggctaa atgcctgaga
     8221 ccagacccgt ggctgtcccc acccaacaca gcctcagccc agaaagtgcc cttgctttgc
     8281 ttcctcttct cagaagggga ctctcagaga cacattcagg agaggacagg gaaggacacg
     8341 tgcccaggga agctgcaaga ctccctgagc tatctccagc cacggcggaa agcattgttg
     8401 ctcttatcag cacattagta ttaaaaggct gttgcttgag cagagatgga gatgaaacag
     8461 acgcaaggca tctagctcag gacagaatcg gattcctcat gtgaaggaac ctcagagctg
     8521 atctgtttaa aatgactcat catacagaca gggaaatgac agtgcgggga gggaatctag
     8581 ccagtgaggg tacatttagg acttgaactt gagccttctg ggccttctga ctccgtgtcc
     8641 agtgttcttt cttctcacca cagctgcctc agcaggtgtt gcctgagatc tgtcctgctt
     8701 gtcaccatgt cacctctctc agccattaca ctatgttccc agaacctccc agcaaacaac
     8761 ccaggctttt cctgagtctg agatcagccc gtctgcgata acctgtgatt agctcagtca
     8821 tggagtccca ggcagcccga ggctggaagg tagaaggatg agcccgtagg tgtttgctac
     8881 tgtgaaacct ggactgggcc ccgggatggt gtctcttgat tcatcacacg cctggccttc
     8941 agcataccgt attcctcaga agggagggta aggaggggtg tctctgtcca tcctgccctt
     9001 gaccgccaag tctgcaggta ttgaggggta gcagggccca ggcaagtctc ccaccctggg
     9061 atctgccgag ctggagttta gcagatgtaa agacttctat gaagcaataa acacaaaagt
     9121 ctgggagaag ctatccagag ttttgtgcaa tcctgtttct ttttcaaagc tgtagcagat
     9181 caggtgaccc tgacctgctt gctcttctaa caaatgtgat gtcttcagtc agtgtttgcc
     9241 tctacccggc ttcccagctc tttgccaact tcttctgagc tgagttatca gtcgcctgtg
     9301 acacgcccat tctctgtcct ggtcccgccc ccaaccatgg tggatcctgg aggacctcgt
     9361 acccctcccc tcccccacat atgcatctct cccttccgac atggtgttcc ctgtgtctcc
     9421 atgctgccca cattcctaag cacatgagtt ggtctaatct ttttttccat tggttacctt
     9481 taactctcca tggcaggctc actttaatcc ctcacatcat gtccttccct tcttgtgagt
     9541 tatttgtcat taaccgacat tgtcagcgac agacgtgtat ctgagggtgt catgcacgac
     9601 cttcagcagg gacttctggg ccatggagga ctgtctaata catggactta taaactggct
     9661 gcatgagcaa tgaaaaggcc aaattattct gatttttttt ttgaatcact gtaaaaaaaa
     9721 aagctgattt cttttgtata gagaacacta aacgtataat aaaagttgtt caaaatggaa
     9781 aaaaaaaaaa aaaaa
//
