LOCUS       NM_001077354           10961 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus neurite extension and migration factor (Nexmif), mRNA.
ACCESSION   NM_001077354 XM_205178 XM_897325 XM_920303 XM_920319 XM_985471
            XM_985501
VERSION     NM_001077354.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Mount,R.A., Athif,M., O'Connor,M., Saligrama,A., Tseng,H.A.,
            Sridhar,S., Zhou,C., Bortz,E., San Antonio,E., Kramer,M.A.,
            Man,H.Y. and Han,X.
  TITLE     The autism spectrum disorder risk gene NEXMIF over-synchronizes
            hippocampal CA1 network and alters neuronal coding
  JOURNAL   Front Neurosci 17, 1277501 (2023)
   PUBMED   37965217
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10961)
  AUTHORS   Stekelenburg,C., Blouin,J.L., Santoni,F., Zaghloul,N., O'Hare,E.A.,
            Dusaulcy,R., Maechler,P. and Schwitzgebel,V.M.
  TITLE     Loss of Nexmif results in the expression of phenotypic variability
            and loss of genomic integrity
  JOURNAL   Sci Rep 12 (1), 13815 (2022)
   PUBMED   35970867
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10961)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10961)
  AUTHORS   Gilbert,J., O'Connor,M., Templet,S., Moghaddam,M., Di Via
            Ioschpe,A., Sinclair,A., Zhu,L.Q., Xu,W. and Man,H.Y.
  TITLE     NEXMIF/KIDLIA Knock-out Mouse Demonstrates Autism-Like Behaviors,
            Memory Deficits, and Impairments in Synapse Formation and Function
  JOURNAL   J Neurosci 40 (1), 237-254 (2020)
   PUBMED   31704787
  REMARK    GeneRIF: ale NEXMIF KO mice demonstrate reduced sociability and
            communication, elevated repetitive grooming behavior, and deficits
            in learning and memory. Loss of NEXMIF/KIDLIA expression results in
            a significant decrease in synapse density and synaptic protein
            expression. Male KO animals show aberrant synaptic function as
            measured by excitatory miniatures and postsynaptic currents in the
            hippocampus.
REFERENCE   6  (bases 1 to 10961)
  AUTHORS   Gilbert,J. and Man,H.Y.
  TITLE     The X-Linked Autism Protein KIAA2022/KIDLIA Regulates Neurite
            Outgrowth via N-Cadherin and delta-Catenin Signaling
  JOURNAL   eNeuro 3 (5) (2016)
   PUBMED   27822498
  REMARK    GeneRIF: Knockdown of KIAA2022/KIDLIA leads to altered neuron
            migration and a reduction in dendritic growth and disorganized
            apical dendrite projections in layer II/III cortical neurons. In
            cultured neurons loss of KIDLIA expression also leads to
            suppression of dendritic growth and branching. KIDLIA suppression
            leads to an increase in cell-surface N-cadherin and an elevated
            association of N-cadherin with delta-catenin.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10961)
  AUTHORS   Magome,T., Hattori,T., Taniguchi,M., Ishikawa,T., Miyata,S.,
            Yamada,K., Takamura,H., Matsuzaki,S., Ito,A., Tohyama,M. and
            Katayama,T.
  TITLE     XLMR protein related to neurite extension (Xpn/KIAA2022) regulates
            cell-cell and cell-matrix adhesion and migration
  JOURNAL   Neurochem Int 63 (6), 561-569 (2013)
   PUBMED   24071057
REFERENCE   8  (bases 1 to 10961)
  AUTHORS   Kobayashi,S., Totoki,Y., Soma,M., Matsumoto,K., Fujihara,Y.,
            Toyoda,A., Sakaki,Y., Okabe,M. and Ishino,F.
  TITLE     Identification of an imprinted gene cluster in the X-inactivation
            center
  JOURNAL   PLoS One 8 (8), e71222 (2013)
   PUBMED   23940725
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 10961)
  AUTHORS   Ishikawa,T., Miyata,S., Koyama,Y., Yoshikawa,K., Hattori,T.,
            Kumamoto,N., Shingaki,K., Katayama,T. and Tohyama,M.
  TITLE     Transient expression of Xpn, an XLMR protein related to neurite
            extension, during brain development and participation in neurite
            outgrowth
  JOURNAL   Neuroscience 214, 181-191 (2012)
   PUBMED   22531377
REFERENCE   10 (bases 1 to 10961)
  AUTHORS   Cantagrel,V., Haddad,M.R., Ciofi,P., Andrieu,D., Lossi,A.M.,
            Maldergem,Lv., Roux,J.C. and Villard,L.
  TITLE     Spatiotemporal expression in mouse brain of Kiaa2022, a gene
            disrupted in two patients with severe mental retardation
  JOURNAL   Gene Expr Patterns 9 (6), 423-429 (2009)
   PUBMED   19524067
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL671963.9.
            
            On Jul 27, 2018 this sequence version replaced NM_001077354.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ975302.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-552               AL671963.9         176017-176568       c
            553-608             AL671963.9         75010-75065         c
            609-734             AL671963.9         64265-64390         c
            735-5109            AL671963.9         59240-63614         c
            5110-10961          AL671963.9         52816-58667         c
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 46.48 cM"
     gene            1..10961
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="neurite extension and migration factor"
                     /db_xref="GeneID:245555"
                     /db_xref="MGI:MGI:2148050"
     exon            1..552
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /inference="alignment:Splign:2.1.0"
     exon            553..608
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /inference="alignment:Splign:2.1.0"
     exon            609..734
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /inference="alignment:Splign:2.1.0"
     CDS             656..5203
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="XLMR protein related to neurite extension"
                     /codon_start=1
                     /product="neurite extension and migration factor"
                     /protein_id="NP_001070822.2"
                     /db_xref="CCDS:CCDS53166.1"
                     /db_xref="GeneID:245555"
                     /db_xref="MGI:MGI:2148050"
                     /translation="MDNQQDKVIAASANGDNNLINGVKNNDSEDQEVAMKSFVALEAT
                     TPIQPIPVIQKESPMFPRGLLPPPSKKPCMQSPPSPLALIEAPDHSANSASVNAISLT
                     SGVAKGLNTWSLPNECEKAPFAIMEPAGMSALNGDCLMQPSRTCLGCFMESKEAVDPE
                     PGISLKVSDLNRDYETCAVSDIGIQCINAGENIKYGEQLLSDQLLGFPLHKSRAGDRR
                     ESEKPDIDLEDPTQKSYYEALLLDKCNTEEALLANSNQDWGYFETFISESKIELLDLC
                     SKNELSVNLFSEEDVENYMFDDDESTLGSDVCSLKIRYESFQDNVRDKTTLLMQEDAQ
                     FNFFPSVFTTCPKRESKSGILKQSSDLSQFKVPDVSIIWGEEDKNLDKKKGKEEVHED
                     KSIETKDEKDNGEKPALNNKPCGGLEVEQFKNLKADQLTNSLETSGNFSDDSSFIEVS
                     YDAMGEIKDCSRYMARDTNSGSSSSQQNYGLRAKRKVRYSEDYLYDVDSLEGEKVNER
                     KEWPPGGSKEEDDDEWCPKKRRKVTRKEPPVIIKYIIINRFKGEKNMLVKLSKVDASE
                     TTVNLSENQLSKYAKLSPLKGFWQKKKKQKNSNTDSVKTPLCQKQSFEPGSFEVSFLP
                     PARKRKSKLGNRHRIQRIQSVETSASSKQVSFCSDQKQACNRKEDGVKGTPKSALLTD
                     PSCANGSHLRGLIVSDSVKVKAQDTEFKGPERKVLNKIKFKSEARLKSKKIKAGQENK
                     PVVQMSPVSEDTSSKANLKNEVTPGTSNSSHMSEFHETKVKNSTFLPTTCSSEMPLSS
                     ANVATNIPVIPGGYLQTLLDASDLSNNTSISYFTNHSAEQNEGSLTQTEKAFVPLQSA
                     QDCVLSSSSDSQLQQSSQNFKMEASNFGSLWPDKDTSGSQEFMTEVSREIATNQSSEF
                     EASQVVSMENNLTAITYSPVCLNSDASGCNKVLYASLQDSHLPPEDLYQLCHFNNGEI
                     CFPFQQGPLSTDDDGRLFSFDSMTSLTVSSSNYCSLSLKSCEKDGDDEINDDFLAHCS
                     PKLVIQQSIDEIAPLKESTDLLDISNFTPDKFRHSSLLEMSPPDTPSLSPQSTRCESI
                     KTLGTMKGFQEGVPGSLSTVEKIKWDCNTLSQQAQADDGFTLNSHQFQFHMFNDEDSV
                     GLLQKSPCLSTFDEPAGQINTNSKVSKSRKKTSPGKSGAVSQSSSQKNSRKKSPKASN
                     KGVEKPPSKTSRQVPKSTKKGKYVAAVNGEKMQIGIGHSGGQPNSTSSNAKTLTECIQ
                     HGGPVASMKIPSQKGLSGDWALGKESRPGWNDMSVVTNTNNLLDDDQREFQEPSYILS
                     NIASGMADVQRFMMASMEPLWEPMEHQGESNTFYSPDSNSLKLKTLKILAGTPQESKK
                     KVTNGSSGATKNHRSVKAVSKSNGKAAIGEPGHADMPGSSEDSRSAFFDKKYSNVNTL
                     GNNGPTHKKLYRHKSSSKGLRDEKYKGKRVEREQAHKDEAGTTSFEKLRDSNYNLLKA
                     ETAFGVLPVFEEETHIFQKDI"
     exon            735..5109
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /inference="alignment:Splign:2.1.0"
     exon            5110..10961
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      9908
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
     regulatory      10936..10941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="hexamer: AATAAA"
     regulatory      10940..10945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="hexamer: AATAAA"
     polyA_site      10951
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="major polyA site"
     polyA_site      10961
                     /gene="Nexmif"
                     /gene_synonym="A230051P11; KIDLIA; Xpn"
                     /note="major polyA site"
ORIGIN      
        1 agtagcatca gcagcagcag ccaccgccgc cgccaccacc acagcagtgg cagtagcggc
       61 agtggcagcg tttagcagta gtgttcccag cgctagcggc agtcgcaacc gccctccgcg
      121 tctccgtctc gcggacagcc gcagccaccg cgtgcagagc gggctccggg caccgccgcg
      181 cgcagccggc gagccgggca gtgctggcgc gggcgagcgg cgcggtgaga ggagcgctgt
      241 cctgggagcc ggcgggcggc tgtggagcct ctgagtttcg gcgcccgagc ccgacagctc
      301 tgcctccatc tagtgcggcg gctgcggcgg agccagagtg agcgagcgga gcgaggcgcg
      361 tccacctggc gggccccgcg catggagcag aggcagagga gacggcagtg agcgggagca
      421 gcggcggcag ctctagcagc tccggcgacg gcgacggcgg cggcagccca gccttgactg
      481 cgaccgtccc agctcgggac gccggcagca aggacccacc agcgacgtgc gcctcgggag
      541 gcggcctgcg aggtgctctg caagctgaga actgacttca gtctcctcta cttttttatt
      601 agtttcctgt tggacaacat ttgaaattgg aatgaaacag taagaggcta cattcatgga
      661 taaccaacaa gataaagtta ttgctgcctc agccaatgga gacaacaatc tgattaatgg
      721 ggtcaaaaac aatgactcag aggaccagga agtggcaatg aagtcattcg tagctctaga
      781 agctactact ccaatccagc ctataccagt gatacaaaaa gagtccccga tgtttcccag
      841 gggccttctt cctccgccat ctaagaagcc gtgtatgcag agccctccat ctcctttggc
      901 cctgattgaa gcacccgatc attctgctaa tagtgcttct gtgaatgcca tctccctcac
      961 atctggagtt gcaaaaggcc tgaacacatg gtcactgccc aacgagtgtg agaaagctcc
     1021 atttgccata atggaacctg caggcatgtc agctctgaac ggagactgcc tcatgcagcc
     1081 aagcaggact tgcttaggtt gcttcatgga atcaaaagaa gcagtagatc ctgaaccagg
     1141 gatcagtctg aaagtcagtg atctaaatcg agattatgaa acatgtgcag tctctgacat
     1201 agggattcaa tgcattaatg ctggggaaaa cattaagtat ggagagcagc tgctttcaga
     1261 ccagctgtta ggcttccccc tgcataagtc aagggcagga gatagacgag aatctgagaa
     1321 acctgacatc gacttggaag atccaactca gaaaagttat tatgaggcat tactattaga
     1381 taagtgcaac acggaagaag ctttgctggc aaattccaat caggattggg gttactttga
     1441 gacttttatt agtgagagta agattgagct acttgacctc tgttccaaga atgaactgtc
     1501 tgttaacctt ttctctgaag aagatgtgga gaactatatg ttcgatgatg atgagtcaac
     1561 cctgggcagt gatgtctgct ccctcaaaat tcgatatgag tcctttcagg acaatgttcg
     1621 agacaagacc actcttctga tgcaggaaga tgcccaattc aattttttcc ccagtgtctt
     1681 tactacgtgt cccaagcggg aatctaagag tggaatcctc aagcagagca gtgatctttc
     1741 tcaattcaag gtccctgatg tgagcatcat ctggggagaa gaagataaaa acttggacaa
     1801 gaagaaaggc aaagaggaag tacatgaaga caaaagcata gagacaaaag atgaaaaaga
     1861 taatggagaa aagcctgcat taaacaataa accatgtggt ggtcttgagg tagagcaatt
     1921 taagaaccta aaggctgacc aacttactaa ttccctggag acatcaggga atttcagtga
     1981 tgacagttcc ttcattgagg tctcatatga tgccatgggg gagatcaaag actgtagccg
     2041 ctatatggct cgggacacta attctggaag ttcatcctcc cagcagaact acggactgcg
     2101 agccaagaga aaagtcagat acagcgaaga ttatctatat gatgttgact cgctagaggg
     2161 ggaaaaagta aatgagagaa aggaatggcc tccaggtggt tccaaagaag aagatgatga
     2221 tgaatggtgt cccaaaaaga gaagaaaagt aacacgtaaa gagccccctg ttattattaa
     2281 atatatcatt atcaatcgtt ttaaaggtga gaagaacatg ttggtgaagc taagtaaagt
     2341 agatgccagt gaaacaacag taaatctgag tgagaatcag ctcagtaagt atgccaagct
     2401 atcacctttg aaaggattct ggcaaaagaa gaaaaagcag aaaaacagca acacagactc
     2461 agtcaaaaca cccttatgcc aaaagcaaag ctttgaacca ggtagctttg aggtgtcatt
     2521 cctgcctcct gctcgtaaac gaaaatctaa gcttggcaac agacacagga ttcaaaggat
     2581 ccaatctgtg gaaacttcag caagtagtaa gcaggtttca ttctgcagtg atcagaaaca
     2641 agcttgtaat cgcaaagaag acggtgtaaa agggacacca aaatcagcac ttcttactga
     2701 cccaagctgt gccaatggat cacatttacg tggcctcata gtctccgact cggttaaagt
     2761 caaagctcaa gatacagagt tcaagggccc agagaggaaa gtgcttaaca aaattaaatt
     2821 taaaagtgaa gctaggttaa aatccaaaaa aatcaaagct ggacaagaaa acaaaccagt
     2881 tgttcaaatg agccctgtct cagaagacac atcctccaag gctaatttaa aaaatgaagt
     2941 cactcctgga acctcaaaca gttcccatat gtctgaattt cacgagacaa aagttaagaa
     3001 ttccactttc ctaccaacca cctgctcttc tgaaatgcct ctatcatctg ctaatgttgc
     3061 cactaatata cctgttattc ctggagggta tctgcaaaca ctgttagatg cttctgactt
     3121 atcaaataat actagtatct catacttcac caaccattct gcagagcaaa atgaaggcag
     3181 cctcactcaa acggaaaaag catttgtacc tctccagtct gctcaagact gtgtgctgtc
     3241 ttcatcttct gattctcagc tgcaacaatc atctcagaac ttcaaaatgg aagcaagcaa
     3301 ctttggaagt ttgtggcccg acaaggatac atctggctcc caggaattca tgactgaagt
     3361 ctcaagggag atagccacaa accaatctag tgaatttgaa gcttcccaag ttgtctccat
     3421 ggagaataac ctcacagcta taacatacag tcctgtatgc ctcaatagtg atgccagtgg
     3481 ttgcaacaag gttctgtatg cctctttgca ggactcacat ctcccacctg aagacttgta
     3541 tcaattgtgt cactttaata atggagagat ctgctttccg ttccagcagg gcccactcag
     3601 tacagatgat gatggccggc tctttagctt tgattcgatg acttccctta ctgttagttc
     3661 aagcaattat tgttccttaa gcttgaaatc ctgtgaaaag gatggtgatg atgaaatcaa
     3721 tgatgacttt ctggcccact gcagccccaa actggtgatc caacagagca ttgatgaaat
     3781 agcaccacta aaggagtcca ctgacctcct tgacatctcc aacttcactc ctgacaagtt
     3841 ccgacactct tctctcttag aaatgtctcc cccagacacc cccagtcttt ctcctcagag
     3901 taccagatgt gagagtatca aaacactagg aacaatgaaa ggattccaag aaggtgtccc
     3961 aggatcgttg agcaccgtgg aaaaaatcaa gtgggactgt aatacccttt cacaacaggc
     4021 ccaagcagat gatggcttta cattaaatag ccatcagttt cagttccata tgttcaatga
     4081 tgaggactct gttggcctgc tccaaaaaag cccttgcctg tcaacatttg atgagccagc
     4141 tgggcaaatt aataccaaca gcaaagtgtc aaaatcaaga aagaaaactt cacctggcaa
     4201 gagtggggct gtaagccaaa gttcttctca gaaaaattcc aggaaaaaat cgccaaaagc
     4261 aagcaacaaa ggggttgaaa agccacctag caaaacctcc cgccaggttc ctaaatcaac
     4321 aaagaaaggg aaatatgtgg ctgctgtcaa tggagagaaa atgcaaattg ggattggcca
     4381 tagtgggggc caacccaata gtacatcctc caatgcaaag acactgactg aatgtattca
     4441 gcatggtggt cctgttgcct ctatgaagat accaagtcag aagggacttt ctggagattg
     4501 ggctttggga aaggaaagca ggccaggctg gaatgacatg agtgtagtca ctaataccaa
     4561 caaccttctg gatgatgacc aacgagagtt tcaagagcct tcctatatct tatccaatat
     4621 tgcctctggt atggcagatg tgcaaagatt catgatggcc tccatggagc ccctttggga
     4681 acccatggag caccaagggg agtccaacac attctactcc cctgattcca atagcctaaa
     4741 attaaaaacc ctcaaaatac tggctggaac accacaagaa tctaagaaaa aggttactaa
     4801 tgggtcttca ggagctacta agaatcacag gtcagtcaaa gctgtgagca aaagcaacgg
     4861 gaaagcggca attggtgagc ctggtcatgc agatatgcct ggttccagtg aggactctcg
     4921 atctgccttc tttgataaaa agtacagtaa tgtgaacact ttaggcaata atggaccaac
     4981 acacaaaaaa ttatatcgtc acaaatccag ctccaagggc ctgcgagatg agaaatataa
     5041 gggaaagcgc gtggaacgag aacaggccca caaggatgag gctggaacaa cttcttttga
     5101 aaaactgagg gattccaact acaatctcct aaaagcagaa acagcatttg gggttttacc
     5161 tgtgtttgaa gaagagactc acattttcca gaaagacatt tgatgtttgt gttttctttc
     5221 tttcaaaata agctttgtgg tatgtatgtt gacctgtaag tgcttacaag aaaagaattt
     5281 taaatcgtgg gaataagtct ttgaaatcaa actttaatct gatgttctgt gtaaaagact
     5341 ttaaaagtca tacagttgca cgagtagttt atgcactgtt tgcccacaag gaaaaaaatg
     5401 gaaaactttg tgccaaacta caaataagta actgtattgt atatagtttt acttctatat
     5461 caacatttgg ttgtgagtat ttgggggact tgtccctgtt aatttactag aaacattcca
     5521 gtgattcttg ctattaacca cccccacttt tgtggatagc acctatagat cacagtggaa
     5581 aatatgttgt gttatacagt ttaccaaaac aaaggataat gtttgaattg tgccaaattt
     5641 tcttacttca tctcacagat tcaccccaca gtttaaaact cattcatgat tattaaatat
     5701 atatatacat atacatccat acattctttt aatgttttaa actctactat cctgtgtgga
     5761 aatatgaaca gttaaacaag gcacaataaa gatgtgtttg ttccatcatc taaggattaa
     5821 aatgttggaa aagcttaggt cacaacagct aagtccatca gggacagagt atacacattt
     5881 acagaacctt gcacaaggaa atcaaacagc tgctttgaaa gttggttttc caaagcaaaa
     5941 tctgtagcag aaaagaattt aaagtgcaaa gttatattgc tgatacttta tatctcagtt
     6001 ttatatattt tataatagcc tgggataaat tataaaataa ctataaaatt gacactaata
     6061 ataaataaaa tacatagatt gcttttattt aagtagtttc ctaaatgttt tattccagtt
     6121 atgtcaaaag tgcactcaca acatggcttt agtgttcttt aaatgttgat cttgcaatgc
     6181 caatgtcatt ttttaaaatg acatctcaga ttgacagaat ttttcttcct atgattaaat
     6241 acatatacca ccaggtaaag ctgaacaata tgtggaaata actaatttag gccatctaca
     6301 tatagtctga ggatgactac tcttttgata tcacacacta aacataaatc tcaattttta
     6361 attacctttt aaattcatat ttttggttca accctccctt tcactactta gaaacttcac
     6421 agttcccaca ttcctcacta ctagcctttt cttccccttt cttcaccact tgacttatac
     6481 catttgatct cttttccttg cacagttaag gcctatgtgc ctaatgactg atgcattgtg
     6541 gcacagatga gataatggat gtgaaagcgc tttgtagatc ataaagtgct ataccaatat
     6601 aggggactaa tattattaat gttgttggtt gttgttgttg ttactattct tactaatatt
     6661 gttactaacc tattaacttg tgaatataga ggctggggtg ggagggagga ggataagggg
     6721 gtgggagaag gggacttaat aaaacaggga agatgtttca aaagacattt ttgaataaaa
     6781 tatgagcaac ctacctagtc attgacacct aaatatattc acaaacagag aagcaatgtt
     6841 ttcaagtagt cagagagaaa tggaagggag cctatcatgt atataaacat acacatttcc
     6901 cagcccatga ttcctttcac cagcttactc tactacttac agctaggtag gaaaatgaac
     6961 aagcccatga tctagattgt gtgtgttctc agcatcagtc tcttttgtct gtggggatga
     7021 gtgggtgagg agaaatctgt gaggagagtt caggcacatt cctaggcttt gacactgtgt
     7081 tcaatgagcc ctggaaaatg atttagtggg aaggcaccaa ctgaaacaag tctcttgccc
     7141 tacaatgacc atccaaaaga gaaaataatc aaactagaac actttctttt gtttcttggt
     7201 actctaatag ctagctgttt gtattaaatg tgatggctac ttcctaggaa ctggtctttg
     7261 ttcagttttg gtttttgtct tgttttgcag ctgacctttt gcttagaatc atcacctcag
     7321 cctgtattgt gatccccaag actccagacc tcaaactcat cttgcttggt tttaggaatg
     7381 gagtgctttc tatgtaacca aaaagtggaa attaaagaag aaataaagaa aatgttctat
     7441 gagtataatg tgtttttata aaagtaaata tgggagcagt tgtcccagat ttttttaaag
     7501 tatcatgatg ttcagctttg gagaatgggg taagactggg gtaggatagc ttgaaactgt
     7561 ttctaacagt ttcattgtca gaacttgatc atcagtcctt tggagtggat actctttcag
     7621 ttgctcctat ggagcccact aggggccaat tttagggcat ggttcatatc tttctccaaa
     7681 gagaactgcc attttaaagc acctttttat cggcctgcca tgcattgtat gttcttaaca
     7741 tataaactag tgacttgtaa ccagtcattt tgtattgaaa cataacaaga aagtgaattt
     7801 gaggagtgtg caatagacca aggcagagat gttattgtac actatgctct acattgttga
     7861 gcactttgca ttctgttggt cctgcctctg ctctcagaat tattttcctt tctgtcacaa
     7921 catagtaggc caaacttact ggagatctct tattcaacca gctgccttag tggagtgagg
     7981 accaatgtat gaaaatagtg ttttgcccta tcactgattt cttcaactca aaaaccctgg
     8041 attttccttg atctgaaatt tctgactacc cccttctaac ctctcctttc tgagaagctg
     8101 catcctatta atgcctcctc attggagtct catttaatct ttactttcct ggaaattata
     8161 tcctcccttg ttcttctttc ttgactttta tcagtcttag aatttttctt cttgtgtagt
     8221 attctattca atatcccaaa ctgtcaaagt ttaagaacta cagctagagg ctttcttttg
     8281 gaagtttaga ggactacctt ccctgtctta ttcactcaaa atacaaatgc acgagcacat
     8341 gcacacatag cattgagaaa agactgaaac tgctttctct tgaaggtgtg tgataacagg
     8401 tccaatctct tattgtggtc aggtgctgta ctacatggag cagagtgcag atacaatggc
     8461 cccagccaag ggtcttcaag acattgaggg ttgtgttggg actagagtaa atagcttgaa
     8521 cctgtggcca tcctctatgc ctctccagtg tctcagatct cagctgcttc acatatgctg
     8581 ctccctcaca gctttcccta ctgacttctt gctgctctgt gcaattatat gttaaggcct
     8641 tatcttttcc tatatctttt caaactttct gcactacttc attcatcact tctttagcca
     8701 tctctctgaa gtccttggtg ctaggtgggc ttttgtgaca aaaattttca ccttctctga
     8761 ctcttttctt tgctgggaag aatcgttggg gggtagggaa cacaaaggag ggtatgtgtg
     8821 tgtgtaagag agaagcataa aggatgcttc aaatgctgta atcaactccc atcctgtgtt
     8881 atttcacgtg aggtcttgca gttctagtga atttccattt tagtggcttt gaaatagtgt
     8941 ttactgcgta aaggagaaaa gcatatccaa ggacctcttt gccttgctct taccaaactg
     9001 aactagtgtt ttggagatct acaatttata gatgcactca gaacagttct gagaaatgag
     9061 gagagttaga gttgagtttc tatgctgaag aggtaaatgg aacactagct agtcctagct
     9121 gttgctagaa ctagaaatgt ttgttttctc ctgtttgact gaaaggttgt caaattattc
     9181 attttcattt agggctttta atgctgccag tcataacaga aatatctcct tttgtaaaat
     9241 aaaactaatg caagttttgt tttttaatca taaaattctc taagccaata ttatgtttag
     9301 aaggcttagt accaaaatca aacattttca tgacaaacag agatcttgtc aacataatgt
     9361 aggccaggga ttttatagag atttctcaaa gacccgagtt aaggacagaa attagttgag
     9421 atgtttagtt tgggaaacac tactaccatc agcttaagag tcctcagcca gcatggagtt
     9481 attttggtga ctatgcaggc taccctttaa gacacagttt tcactcaaca ttaaacagta
     9541 gctacagtca tgatgcagag atcccattgc agctatatga tacttgaggt gtactcttgg
     9601 tttgagaatc atagctaaat gctaaaacac tggggaaaga cactataaga gagtggctta
     9661 gtggaaagtt ttaaggaatg aatgaggctt ttaaaaatca gtgtttgggt aaatcaatac
     9721 taaatacact aaagaaacac tcacccatac aatatgaaaa ggatgtctgt gtatccaaca
     9781 agctcagggt tctctgggcc cactgaaaat gaagaaaggt aaccaggcat ggaggctcat
     9841 gcctggaacc ctaggcagga ttgttgggag ttccagacca gtctggtcta tagtgtgaga
     9901 cccagtctga aaaataaaaa tataaaagaa agaaggagga tgaggaagga aggaagaaat
     9961 gaatgaatga acaaatgaaa gaatcaagga aggaagggag ggagggaggg agggagggag
    10021 gaaggagata ctgaaatcat tctgcctctt tgagagatgc ctagttttac agcacaaaag
    10081 aaaaaggctt gaatgaaaac taaaaggggc ctttttgttt ttcactccca gggaggtgga
    10141 gagcttcaga gtctccactg ggatagctgt agcactgact ggagtttgct gcctacagag
    10201 ttttgaatgt acttactaga ctttagtttc catagaactg aatgcacctc ttcagtaagt
    10261 gaaatgcttt atggtgctaa agaaaatgat tatccccatt tttcttcttc gccaagcccc
    10321 ttaagcattt aagaaaattt aaagtaaaag catttagaga ccctttttat gaatgtaacc
    10381 tgtttctgtt tgacacatga cactgcaact ttaaagttag gaaattattt gcctttttct
    10441 tttagaatta agctttataa ataatctgta gagtcaattg aagtataaag cttaaggatc
    10501 actcttaaga cgcctacaaa atcttgtata tttttaagtg tgccaatttg taacatattt
    10561 tgtaagtgta tatttttctt agaaaagtgc tgtgaagtgt ctgtatgtga gagttttcct
    10621 tttttctgca ccactatgtg ctaataaaag gatatttact tcaaagtttc tggttttaga
    10681 aaccacaata caaagaaaaa acacttttgt tgggaatttt gtaacaaaaa atatgttgtg
    10741 aaagagtgta gtgatcttgt gcaaagaaaa tgaacatttg tgagcttctt gctgttttat
    10801 agaatttctt gtaaattatt cttgtaacac ctctggtttt gttgttcttg ttgaaaaaag
    10861 ttttaaatat ttgtgactga atgtatggtg aaactgtcat aatgttacct agctgagttt
    10921 tgttattgtt tatggaataa ataaaagaaa agtttaaaat a
//
