LOCUS       NM_019671               3995 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus neuroepithelial cell transforming gene 1 (Net1),
            transcript variant 1, mRNA.
ACCESSION   NM_019671
VERSION     NM_019671.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Wang,S., Matsumoto,K., Mehlferber,M.M., Zhang,G., Aronova,M.A. and
            Yamada,K.M.
  TITLE     Microtubule-dependent apical polarization of basement membrane
            matrix mRNAs in mouse epithelial cells
  JOURNAL   Cells Dev 177, 203898 (2024)
   PUBMED   38103869
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Wang,S., Wu,X., Zhang,M., Chang,S., Guo,Y., Song,S., Dai,S., Wu,K.
            and Zeng,S.
  TITLE     NET1 is a critical regulator of spindle assembly and actin dynamics
            in mouse oocytes
  JOURNAL   Reprod Biol Endocrinol 22 (1), 5 (2024)
   PUBMED   38169395
  REMARK    GeneRIF: NET1 is a critical regulator of spindle assembly and actin
            dynamics in mouse oocytes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3995)
  AUTHORS   Sapir,T., Kshirsagar,A., Gorelik,A., Olender,T., Porat,Z.,
            Scheffer,I.E., Goldstein,D.B., Devinsky,O. and Reiner,O.
  TITLE     Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the
            developing mouse cortex
  JOURNAL   Nat Commun 13 (1), 4209 (2022)
   PUBMED   35864088
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3995)
  AUTHORS   Robinson,R.A., Griffiths,S.C., van de Haar,L.L., Malinauskas,T.,
            van Battum,E.Y., Zelina,P., Schwab,R.A., Karia,D.,
            Malinauskaite,L., Brignani,S., van den Munkhof,M.H., Dudukcu,O., De
            Ruiter,A.A., Van den Heuvel,D.M.A., Bishop,B., Elegheert,J.,
            Aricescu,A.R., Pasterkamp,R.J. and Siebold,C.
  TITLE     Simultaneous binding of Guidance Cues NET1 and RGM blocks
            extracellular NEO1 signaling
  JOURNAL   Cell 184 (8), 2103-2120 (2021)
   PUBMED   33740419
REFERENCE   5  (bases 1 to 3995)
  AUTHORS   Shang,H., Wu,B., Liang,X., Sun,Y., Han,X., Zhang,L., Wang,Q. and
            Cheng,W.
  TITLE     Evaluation of therapeutic effect of targeting nanobubbles
            conjugated with NET-1 siRNA by shear wave elastography: an in vivo
            study of hepatocellular carcinoma bearing mice model
  JOURNAL   Drug Deliv 26 (1), 944-951 (2019)
   PUBMED   31544556
  REMARK    GeneRIF: TNBS conjugated with NET-1 siRNA inhibited tumor growth.
REFERENCE   6  (bases 1 to 3995)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 3995)
  AUTHORS   Hwang,S.Y., Oh,B., Knowles,B.B., Solter,D. and Lee,J.S.
  TITLE     Expression of genes involved in mammalian meiosis during the
            transition from egg to embryo
  JOURNAL   Mol Reprod Dev 59 (2), 144-158 (2001)
   PUBMED   11389549
REFERENCE   8  (bases 1 to 3995)
  AUTHORS   Dobrosotskaya,I.Y.
  TITLE     Identification of mNET1 as a candidate ligand for the first PDZ
            domain of MAGI-1
  JOURNAL   Biochem Biophys Res Commun 283 (4), 969-975 (2001)
   PUBMED   11350080
REFERENCE   9  (bases 1 to 3995)
  AUTHORS   Alberts,A.S. and Treisman,R.
  TITLE     Activation of RhoA and SAPK/JNK signalling pathways by the
            RhoA-specific exchange factor mNET1
  JOURNAL   EMBO J 17 (14), 4075-4085 (1998)
   PUBMED   9670022
REFERENCE   10 (bases 1 to 3995)
  AUTHORS   Alberts,A.S., Bouquin,N., Johnston,L.H. and Treisman,R.
  TITLE     Analysis of RhoA-binding proteins reveals an interaction domain
            conserved in heterotrimeric G protein beta subunits and the yeast
            response regulator protein Skn7
  JOURNAL   J Biol Chem 273 (15), 8616-8622 (1998)
   PUBMED   9535835
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139323.4, CF167482.1, AK082128.1 and AK139921.1.
            
            On Sep 26, 2013 this sequence version replaced NM_019671.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF094520.1, SRR13422600.1944843.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC139323.4         128660-128675       c
            17-509              CF167482.1         1-493
            510-2885            AK082128.1         386-2761
            2886-3196           AK139921.1         2730-3040
            3197-3995           AK082128.1         3072-3870
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 2.17 cM"
     gene            1..3995
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /note="neuroepithelial cell transforming gene 1"
                     /db_xref="GeneID:56349"
                     /db_xref="MGI:MGI:1927138"
     exon            1..283
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..1943
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanine nucleotide regulatory protein (oncogene); rho
                     guanine nucleotide exchange factor 8; Net1 homolog"
                     /codon_start=1
                     /product="neuroepithelial cell-transforming gene 1 protein
                     isoform 1"
                     /protein_id="NP_062645.2"
                     /db_xref="CCDS:CCDS26216.1"
                     /db_xref="GeneID:56349"
                     /db_xref="MGI:MGI:1927138"
                     /translation="MEPEPAAQKQPRPRRRSRRVSMLSEEPAAGLPADTPGPAANERC
                     SLRRGSSFTFLTPGPHWDFTLKRKRREKDDDAVSLSSLDLKEPSNKRVRPLARVTSLA
                     NLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKSFSRSTVPTPTKRRSSALWSEML
                     DINMKESLTTREIKRQEAIYELSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHI
                     FGDLDAYIPLHEDLLARIGEATKPDGTVEQIGHILVNWLPGLNAYRGYCSNQLAAKAL
                     LDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHR
                     DVQLLEEAILIIQGVLSDINLKKGESECQYYINKLEYLDEKQKDPRIEASKVLLCHGE
                     LKNKSGHKLYIFLFQDILVLTRPVTRNERHLYQVYRQPIPVQELVLEDLQDGDVRMGG
                     SFRGAFGNSDKAKNIFRVRFQDPSPGHSHTLQANDVFHKQQWFNCIRAAIAPFQRAAS
                     PLELQGLPDLHEECEENNPSAGNLRAQRRSCVVPGVMQIDEESALDCGSSVQTVEDTR
                     NMKAQRPQPGLRRARDKAQSGGKKKETLV"
     exon            284..350
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            351..410
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            411..518
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            519..686
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            687..749
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            750..846
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            847..923
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1181
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1352
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1539
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..3995
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3419..3424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /note="hexamer: ATTAAA"
     polyA_site      3452
                     /gene="Net1"
                     /gene_synonym="mNET1; Net1a"
                     /note="major polyA site"
ORIGIN      
        1 gggcagccgc cattttcaaa tccccggatg acggcggtag ccgctctctg ctagaaggcg
       61 acttgctgcc tcgcagaagc tggcgggcat tgtgcccgcc acgatcggtc tcctgggcac
      121 tgggccaccg ccccaccaac cccccttctc cggacatgga gccagagccg gcggctcaga
      181 agcagccccg acctcggagg agaagccgca gggtctcaat gctcagcgag gaacctgccg
      241 cggggcttcc ggccgacaca cccgggccgg cggcgaacga gagatgctcc cttcggagag
      301 gcagctcctt cacattttta acccctggcc cacactggga cttcactttg aaaagaaaac
      361 gaagagagaa agatgatgat gctgtaagcc ttagcagcct tgatttgaag gagccaagca
      421 ataagagagt tcggcctcta gctcgggtca catctttggc aaacttaatc tctcccgtaa
      481 gaaatggagc agtcaggcgc ttcggtcaga ccatacagtc gtttaccctt cgtggtgacc
      541 acagatcccc agcctctgcc cagaagtcat tcagcaggtc caccgtcccc acacccacca
      601 agagaagaag cagtgcacta tggtcggaaa tgctagacat caatatgaag gagtccttga
      661 ccactcgaga gatcaaacgt caggaggcca tatatgaatt gtcccgaggg gaacaggact
      721 tgattgagga tctcaagctc gctagaaagg cctaccatga ccccatgtta aagctgtcta
      781 tcatgtcaga agaggaactc acgcacatat ttggagactt ggatgcttat atacctctgc
      841 acgaagatct gttagcaaga attggagaag caaccaagcc tgatgggact gtggagcaga
      901 tcggtcacat tctcgtgaac tggttaccag gcctgaatgc ctacagaggc tactgcagta
      961 accagctggc ggccaaggct cttctggatc agaagaaaca agacccaaga gtccaagact
     1021 tcctccagcg ctgtctggag tctcctttca gtcgaaaact agatctttgg agtttcctag
     1081 atatccctcg aagtcgcctc gtcaagtacc ccttactgtt aaaagaaatc cttagacaca
     1141 ctcccaaaga ccaccgtgat gttcagcttc tagaagaggc aatattgata atacaaggag
     1201 ttctttctga tatcaacttg aagaaaggcg aatctgagtg tcagtattac atcaacaagc
     1261 tggagtacct ggacgagaag cagaaggacc ctaggatcga agcgagcaaa gtgctgctgt
     1321 gccatgggga gctgaagaat aaaagcggcc ataaactgta cattttccta tttcaagaca
     1381 tcttggtttt gactcggcct gtcacccgaa atgagcgcca cttgtaccag gtttaccggc
     1441 agcccatccc agtccaggag ctggtgctgg aagacctgca ggacggggat gtgcgcatgg
     1501 gtggctcctt ccgaggggcc ttcggcaact cagacaaagc taaaaatatc tttagggtcc
     1561 gcttccaaga cccttctcca ggccactccc acacactgca agccaatgat gtgttccata
     1621 agcagcagtg gttcaactgt attcgcgcgg ccattgcccc cttccagcgg gccgccagcc
     1681 ccttagagct tcagggcttg ccagacctcc acgaagagtg tgaagagaac aacccttctg
     1741 ctgggaacct cagagcccag cgacggtcat gcgtggttcc tggtgtcatg cagatagacg
     1801 aggaaagcgc cctggattgt ggctccagtg tacagacagt ggaagacacc aggaacatga
     1861 aggcacagcg gcctcagcct ggcttacgaa gagcaaggga caaagcacag tcaggtggca
     1921 aaaagaaaga gactttggtg taaagtgtgt attccctgat ggggagctgt ttatttataa
     1981 atgtgtacag tttttcttat aatgggagca tcaagggtta ctctgtacca gaagtaatgt
     2041 gttcatggtt tgggtctctc tctcatcctc tccctctccc ttccccccgc ccccaatcgt
     2101 cgtcatgtca ttgatgttgt ccttgtcgtc ctcgttgtca tcgtcatcgt tgttattaat
     2161 ggcagctaca gatacacaga ttactgttaa attggagact ttaaaaaggt attttcatgg
     2221 attatttgga acatgccaac ttggtgtatt ttatcagaag cagtgtgtgt ggagccggtg
     2281 ctgtcctcac ctggttcccc atgactgagc aaatgtcaca gtgtttgcca ggcttaaacg
     2341 cttgcaaatg ctgagactcc atcagtgttc tctgtgaaac ctcagaggga gcatggaatt
     2401 gtaatagtga cacctacgat ccgcatggaa cagatttgag attgaaatct tttgtattga
     2461 cagtgaagtc ccgcttcctt gagaacaagc gtgaagctgg atgtcttccc cctttgtctc
     2521 aactggaatg aacaaacacc cgtggcagtc tcactgcacg gttcgccttc tgcattcatt
     2581 tattctgcca actatttaat ttttttttta ttgtaaagta gtttttagta tttgctttta
     2641 tttttttaca ttgatgcctt tttaaagtgg catgtccttg aagtttttcc tcctaattgt
     2701 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttt gtgtgtgaat cattaatttt
     2761 atcaagtgaa accaagccat attgtttgag ccaattaaga aaagtgcagt ttttacagtg
     2821 tatcatgtgt ggtaaagtcc catgaaatga cttagtgtat tttagactac tgaaaggaac
     2881 cacaggaagg gttgggtgga agtttttgtg ttgtctgaaa agcaagagag aaggcaggag
     2941 tctgggatag ggaagggaag ggaagagaag agaagagtag cttgtctaaa gtaaattgtc
     3001 tggctgaacc agaggaggag ccaggagcag cctggcctga gggatgtaga aagttgtaga
     3061 aggcagaggg gatgtgaact gtgtttgctt tggtttctca tacctgcaaa tgctacattt
     3121 gaaaagaact gtacagtgtg ttggaaattt gactatatta ggttaattct ggttggtttt
     3181 cttcatttaa aaaaaaaccc gtgattcttg actacaaaat gtttgtgtta aaaatggggt
     3241 catgctcttt acagggggga gaaggacaga gaccccatgt cagcccaggg ttatactggc
     3301 aatcagttca agtcattgtt aaccattata tgtgaaatat atctccagtg gaaatgaaaa
     3361 ttggaaaagg ctgtaaatag atcaatgaaa tgatttcttt gtataaaaga attatataat
     3421 taaaaaaaaa gcacacacat aataacccaa aacatttctc tcctggtggt tatttggggg
     3481 gccatccact gggccatgag ggcttagcta aacctttaat ggttaaggaa atcccaaata
     3541 atttaccctt ttgaaatttg ttgataagaa aagtccatag tttagttctt aggagtcatg
     3601 tgatctttta aggacatgat gtcatctgaa gtcaccattg tattgcagac agctgaccat
     3661 gctcatcttc tggatacagt agtagagcaa atagcaaaca tcttcattaa tgtggttaaa
     3721 tccaaagtct gaggtaggta cctgagcctt gttattttgg aaattcaaat tcagaagggc
     3781 gccttatcca gtgctgtgat ctgcagctta ggaaagcaag acagtggtct tgtctagggc
     3841 tgtcatgtgg agtttaattt ctctcagagt ggctctgttc agcttctaga gttaatggga
     3901 acaaatggtt cagatgtggt aatggaatga tgtttgatct aaaggtatct tgcttggttt
     3961 ctctcgggag aaaataaaca gtcatgacgt tcttc
//
