LOCUS       NM_016701               6143 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus nestin (Nes), mRNA.
ACCESSION   NM_016701
VERSION     NM_016701.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Kawase,K., Nakamura,Y., Wolbeck,L., Takemura,S., Zaitsu,K.,
            Ando,T., Jinnou,H., Sawada,M., Nakajima,C., Rydbirk,R., Gokenya,S.,
            Ito,A., Fujiyama,H., Saito,A., Iguchi,A., Kratimenos,P.,
            Ishibashi,N., Gallo,V., Iwata,O., Saitoh,S., Khodosevich,K. and
            Sawamoto,K.
  TITLE     Significance of birth in the maintenance of quiescent neural stem
            cells
  JOURNAL   Sci Adv 11 (4), eadn6377 (2025)
   PUBMED   39841848
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Clemot-Dupont,S., Lourenco Fernandes,J.A., Larrigan,S., Sun,X.,
            Medisetti,S., Stanley,R., El Hankouri,Z., Joshi,S.V.,
            Picketts,D.J., Shekhar,K. and Mattar,P.
  TITLE     The chromatin remodeler ADNP regulates neurodevelopmental disorder
            risk genes and neocortical neurogenesis
  JOURNAL   Proc Natl Acad Sci U S A 122 (3), e2405981122 (2025)
   PUBMED   39808658
REFERENCE   3  (bases 1 to 6143)
  AUTHORS   Feng,X., Du,X., Yang,X., Chen,C., Liang,Z., Xu,X., Wang,Y.,
            Zheng,J.C., Xia,X. and Liu,J.
  TITLE     miR-185-5p regulates the proliferation and differentiation of
            neural stem/progenitor cells
  JOURNAL   Front Cell Dev Biol 12, 1510746 (2024)
   PUBMED   39703696
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6143)
  AUTHORS   Rehmani,T., Dias,A.P., Applin,B.D., Salih,M. and Tuana,B.S.
  TITLE     SLMAP3 is essential for neurulation through mechanisms involving
            cytoskeletal elements, ABP, and PCP
  JOURNAL   Life Sci Alliance 7 (12), e202302545 (2024)
   PUBMED   39366759
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6143)
  AUTHORS   de Jaime-Soguero,A., Hattemer,J., Bufe,A., Haas,A., van den
            Berg,J., van Batenburg,V., Das,B., di Marco,B., Androulaki,S.,
            Bohly,N., Landry,J.J.M., Schoell,B., Rosa,V.S., Villacorta,L.,
            Baskan,Y., Trapp,M., Benes,V., Chabes,A., Shahbazi,M., Jauch,A.,
            Engel,U., Patrizi,A., Sotillo,R., van Oudenaarden,A., Bageritz,J.,
            Alfonso,J., Bastians,H. and Acebron,S.P.
  TITLE     Developmental signals control chromosome segregation fidelity
            during pluripotency and neurogenesis by modulating replicative
            stress
  JOURNAL   Nat Commun 15 (1), 7404 (2024)
   PUBMED   39191776
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6143)
  AUTHORS   Marcus,R.C. and Mason,C.A.
  TITLE     The first retinal axon growth in the mouse optic chiasm: axon
            patterning and the cellular environment
  JOURNAL   J Neurosci 15 (10), 6389-6402 (1995)
   PUBMED   7472403
REFERENCE   7  (bases 1 to 6143)
  AUTHORS   Dahlstrand,J., Zimmerman,L.B., McKay,R.D. and Lendahl,U.
  TITLE     Characterization of the human nestin gene reveals a close
            evolutionary relationship to neurofilaments
  JOURNAL   J Cell Sci 103 (Pt 2), 589-597 (1992)
   PUBMED   1478958
REFERENCE   8  (bases 1 to 6143)
  AUTHORS   Lendahl,U., Zimmerman,L.B. and McKay,R.D.
  TITLE     CNS stem cells express a new class of intermediate filament protein
  JOURNAL   Cell 60 (4), 585-595 (1990)
   PUBMED   1689217
REFERENCE   9  (bases 1 to 6143)
  AUTHORS   Wolff,G.L., Greenman,D.L., Frigeri,L.G., Morrissey,R.L., Suber,R.L.
            and Felton,R.P.
  TITLE     Diabetogenic response to streptozotocin varies among obese yellow
            and among lean agouti (BALB/c x VY)F1 hybrid mice
  JOURNAL   Proc Soc Exp Biol Med 193 (2), 155-163 (1990)
   PUBMED   2137249
REFERENCE   10 (bases 1 to 6143)
  AUTHORS   Misson,J.P., Edwards,M.A., Yamamoto,M. and Caviness,V.S. Jr.
  TITLE     Identification of radial glial cells within the developing murine
            central nervous system: studies based upon a new
            immunohistochemical marker
  JOURNAL   Brain Res Dev Brain Res 44 (1), 95-108 (1988)
   PUBMED   3069243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC062893.1.
            
            On Jul 19, 2004 this sequence version replaced NM_016701.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062893.1, BC060693.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.78 cM"
     gene            1..6143
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /note="nestin"
                     /db_xref="GeneID:18008"
                     /db_xref="MGI:MGI:101784"
     exon            1..896
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..5705
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /note="intermediate filament protein; intermediate
                     filament-associated protein RC2"
                     /codon_start=1
                     /product="nestin"
                     /protein_id="NP_057910.3"
                     /db_xref="CCDS:CCDS17461.1"
                     /db_xref="GeneID:18008"
                     /db_xref="MGI:MGI:101784"
                     /translation="MEGCVGEESFQMWELNRRLEAYLTRVKTLEEQNQLLSAELGGLR
                     AQSGDASWRARADDELAALRVLVDQRWREKHEAEVQRDNLAEELESVAGRCQQVRLAR
                     ERTIEEAACSRRALEAEKNARGWLSTQAAELERELEALRASHEEERAHLNAQAACTPR
                     RPPAPAHASPIRAPEVEELARRLGEVWRGAVRDYQERVAHMESSLGQARERLGQAVRG
                     ARESRLEVQQLQADRDSLQERREALEQRLEGRWQDRLQATEKFQLAVEALEQEKQGLQ
                     SQIAQILEGGQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGRSSQASLGFPDPKLKL
                     HFLGIPEDQHLGSVLPVLSPTSFSSPLPNTLETPVTAFLKTQEFLKARTPTLASTPIP
                     PMSEAPYPKNAEVRAQDVPHSLLQGGRQQAPEPLWAEATVPSSTGVLPELEEPGGEQP
                     DHFPDDPTSLAPPLNPHHSILEAKDRESSESRVSSIFQEEEGQIWELVKKEAATEVKV
                     ENSLAQEIQESGLDTEEIQDSQGPLQMETLEALGDEPLMSLKTQNHETPGKENCNSSI
                     EENSGTVKSPEKEKQTPLKSLEEKNVEAEKTLENGVLELSKPLGEEEPRMEDQELMSP
                     EHTLETVSFLGKENQEVVRSSEEQNLESLITFKEESQYPLGGPEAEDQMLERLVEKED
                     QRFPRSPEEDQQAFRPLEKENQEPLRFEEAEDQVLERLIEKERQESLKSPEEEDQQAF
                     RLLEKENQEPLRFEDAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFE
                     EAEDQVLERLVEKESQESLKSPEEEDQRTGKPLEKENQESLRSLDENQETIVLLESKN
                     QRPLRSLEVEEEEQRIVKPLEKVSQVSLESLEKENVQSPRYLEEDDHMIKSLLEDKTH
                     EILGSLEDRNGENFIPPENETQGSLRPPEEEDQRIVNHLEKESQEFLRSPEAEEEEEQ
                     VMVRSLEGENHDPLSSVVKEEQMAESKLENESQDSRKSLEDESQETFGSLEKENLESL
                     RSLAGQDQEEQKLEQETQQPLRAVEDEQMTVNPPEKVDPELPKPLRNDQEVVRSLDKE
                     NQESLVSLNEGGMETVKSSETENIESLETVGECLGRRKSVDTQEPLWSTEVTSETIEP
                     LEDETQEPLGCVDENQEVLTPLERESQELRSLGKWNPETVESPGGVEDSQQCLEVEEG
                     PEREQHQESLRSLGEVEWELPGSGSQQRWEDVVEDGEGQEASLGATGVETEDKAELHL
                     RGQGGEEKAVEEGELLQDAVGEAWSLGSSEPKEQRVPAEPLDDLEGQPEQTGTLEVPV
                     AQGMPEATEQDEDRAQAGEQDSVEVTLGLEAARAGLELEQEVVGLEDPRHFAREEAIH
                     PSLGEESVKAKIDQGLEEPGKEPKEAGALDSGIPELPKTSSETLECKGWEESGEGWGE
                     EEASLETSDHEGSHAPQPRPPKTEEDEGLQAALTVPGPKLLEPCSPIPILTDAHELQP
                     QAEGIQEAGWQPEAGTEALGRVEDEPEFGRGEIPEGLQDWEEGREDSEADELGETLPD
                     STPLGLYLKSPASPKWEQAGEQRLFPQGEARKEGWSPAALAAQGLSDPPEEEQQGHDS
                     DLSSEEFEDLGTEASLLPGVPKEVSDHLGQEPPVLQPACWDQGGESDGFADEEESGEE
                     GEEEDADEEEGAESGTQWWGPGPSGGGVKVQDVTQRGDLEHESVGDSGLWDDGLSGAA
                     ANVLVTALETVSQDSAEPSGSEGSESASLEGEEGQAIDHLDAPQEVTSVVPGAGDTFD
                     ISGQGPNLESEQVNGRMENGLEQAEGQVVLHGDEDQGIPLQEQGTLKAPLVGSPVHLG
                     PSQPLKFTLSGVDGDSWSSGED"
     exon            897..1021
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1095
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..6126
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6102..6107
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /note="hexamer: AATAAA"
     polyA_site      6126
                     /gene="Nes"
                     /gene_synonym="ESTM46; Ifaprc2; Marc2; RC2"
                     /note="major polyA site"
ORIGIN      
        1 cgctgggtca ctgtcgccgc tactcccttt ctccccctta aaagctccaa gggccactcc
       61 cttctctagt gctccacgtc cgcttgccct cggggggcca gaccagcgac atggagggtt
      121 gcgtcgggga agaatctttt cagatgtggg agctcaatcg acgcctggag gcctacctga
      181 cccgggtcaa gacgctggag gagcagaacc agctgctcag tgccgagctt gggggactcc
      241 gggcgcagtc cggggacgcc tcctggcgag cccgagccga cgacgagctg gcagccctgc
      301 gggtcctcgt cgatcagcgc tggcgggaga agcacgaggc tgaggtgcag cgcgacaacc
      361 ttgccgaaga gctggagagc gtggcgggcc ggtgccagca ggtgcggctc gcccgggagc
      421 ggaccatcga ggaggcggcc tgcagccggc gcgcgctcga ggcggagaag aatgcgcggg
      481 gctggctgag cacccaggct gccgagctgg agcgcgagtt agaggctctg cgagcgtcgc
      541 acgaggagga gcgcgcgcac ctgaacgccc aggccgcctg tacgccgcgc cgaccccccg
      601 cgccggccca cgcatccccc atccgggccc ctgaagtcga ggagctggcc aggcgcctag
      661 gcgaagtgtg gcgcggggcg gtgcgtgact accaggagcg cgtggctcac atggagagct
      721 cgctgggcca ggcccgcgag cgtctgggcc aagccgtgcg gggcgctcgg gagagtcgct
      781 tagaggtgca gcagctgcag gctgatcgcg acagcctcca ggagcgcaga gaggcgctgg
      841 aacagagatt ggaaggccgc tggcaggacc ggctgcaggc cactgaaaag ttccagctgg
      901 ctgtggaagc cctggagcag gagaagcagg gtctacagag tcagatcgct cagatcctgg
      961 aaggtgggca gcaactggca cacctcaaga tgtcccttag tctggaagtg gctacataca
     1021 ggactctgct ggaggctgag aactctcgct tgcagacacc tggaagaagt tcccaggctt
     1081 ctcttggctt tcctgacccc aagctgaagc tgcatttcct tgggatacca gaggaccagc
     1141 acctgggatc tgtgctccct gtcctcagcc caacatcctt ctcttccccc ttgcctaata
     1201 cccttgagac tcctgtgaca gcctttctga agacacagga attccttaag gccagaaccc
     1261 ccaccttggc cagcactccc atcccaccta tgtctgaggc tccctatcct aaaaatgcag
     1321 aggtcagagc ccaggatgtc ccccattccc tgctccaggg tgggaggcaa caggctccag
     1381 agcctctttg ggctgaggcc acagtgccca gttctactgg tgtcctccca gagctggagg
     1441 agcctggggg cgagcagccg gaccacttcc ctgatgatcc aacctcctta gccccacccc
     1501 tcaaccctca ccactctatt ttagaggcta aagatagaga atccagtgag tctagagttt
     1561 ctagcatatt ccaggaagaa gaagggcaaa tctgggaact tgtaaagaaa gaagcagcca
     1621 cagaggtaaa agtagaaaac agcttagcac aggaaataca agaaagtggt ctggacacag
     1681 aagaaatcca ggattcccag ggacctttgc agatggaaac cctggaggct ctaggagatg
     1741 agccactgat gtctctgaaa acccagaacc atgagacccc aggaaaggag aattgcaatt
     1801 catctataga agagaactcg gggacagtaa aaagcccaga aaaagaaaaa caaacaccac
     1861 tgaagtcttt agaagaaaag aatgtagagg cagagaaaac tctagaaaat ggggttcttg
     1921 aactatctaa acctttagga gaagaagaac caagaatgga ggatcaagaa ttaatgtctc
     1981 ctgaacacac actagagaca gtttcatttc taggaaagga aaatcaggaa gtagtgaggt
     2041 cttcagaaga acagaactta gaatcattga taacttttaa agaggagagc caatacccac
     2101 tgggaggtcc agaagccgag gaccagatgc ttgaaagact ggtagagaaa gaggatcaga
     2161 ggttcccaag gtctccagaa gaagaccagc aggcgtttag acctctggag aaagagaatc
     2221 aggagccact aagatttgaa gaagcagagg accaggtgct tgagagactg atagaaaagg
     2281 aaaggcagga gtccctgaag tctccagaag aagaggacca gcaggcattt agacttctgg
     2341 agaaagagaa tcaagaacca ctaaggtttg aagacgcaga ggaccaggtg cttgagagac
     2401 tgatagaaaa ggaaagacag gagtccctga agtctccaga agaagaggac cagcaggcat
     2461 ttagacttct ggagaaagag aatcaagaac cactaaggtt tgaagaagca gaggaccagg
     2521 tgcttgagag actggtagaa aaggaaagtc aggagtccct gaagtctcca gaagaggagg
     2581 accagaggac tgggaagcct ctagaaaaag aaaatcagga atctctgagg tctcttgatg
     2641 aaaaccagga gacaattgta ctgctagaaa gcaagaacca gaggccactg agatctctag
     2701 aagtagaaga ggaggagcag agaattgtga aacctctaga aaaagtgagc caggtctccc
     2761 tcgaatctct cgaaaaagag aatgtgcagt caccaaggta tctggaagaa gatgaccaca
     2821 tgattaagag cctgctagaa gacaagactc atgagatcct gggatctctt gaagatagaa
     2881 atggggagaa ctttatacca cctgaaaatg agacccaggg ttcattgagg cctccagaag
     2941 aagaggacca gaggattgtg aaccatctag aaaaagaaag ccaggagttc ctgaggtctc
     3001 cagaagcaga ggaagaagaa gagcaggtga tggtgagatc tctagaagga gagaaccacg
     3061 acccactgag ctctgtggtg aaagaggagc agatggctga gagcaagcta gagaacgaga
     3121 gtcaggactc caggaagtct cttgaagatg agagccagga gacctttggg tctctggaaa
     3181 aagagaatct agagtccctg aggtctctag caggacagga ccaagaggaa cagaaactcg
     3241 aacaagagac ccagcagcca ctgagggctg tagaagatga gcagatgaca gtgaaccctc
     3301 cagaaaaggt ggatccagag ttaccaaagc ctcttagaaa tgaccaggaa gtagtcagat
     3361 ctcttgacaa ggagaatcaa gagtcactag tgtcactgaa tgaaggaggt atggagacag
     3421 tgaagtcttc agaaacagag aacatagaat cactggagac tgtgggagag tgcctgggaa
     3481 gaaggaagtc tgtagatact caagagccat tgtggtctac ggaagtgact agtgagacaa
     3541 tagaacctct agaagatgag acccaagaac cactggggtg tgtggatgag aaccaagagg
     3601 tgctgacacc ccttgaaagg gagagtcaag aactgagatc tctgggcaag tggaacccag
     3661 agactgtgga atcaccagga ggggtggagg acagtcagca gtgcctggaa gtggaagagg
     3721 gcccggagag ggagcagcac caagagtctc tgaggtctct gggagaggtg gaatgggagc
     3781 tgcctggatc tggaagtcaa cagaggtggg aggatgtggt ggaggatgga gaaggtcagg
     3841 aagcatccct gggggctaca ggagtggaaa ctgaggataa ggcagagttg catctgaggg
     3901 gccaaggtgg ggaggagaaa gctgtagagg agggagagct gctgcaggat gctgtggggg
     3961 aggcctggag tctggggagc tcggagccca aggagcagag ggtccctgct gagcccctcg
     4021 atgacctgga gggacaacca gagcagacgg gaaccctaga ggtcccagtt gctcagggaa
     4081 tgccagaggc aacagagcaa gatgaggaca gagcccaagc aggtgaacaa gactccgtag
     4141 aggtgaccct tgggttagag gctgccagag ctggactgga actcgagcag gaagtggtag
     4201 ggctagagga cccaaggcat ttcgccaggg aggaggccat tcacccatcc ctgggggagg
     4261 aaagtgtgaa ggcaaagata gatcagggct tggaagagcc tggaaaggaa ccaaaagagg
     4321 caggtgctct ggactcaggc atccctgaat tacccaagac tagcagtgag actctggaat
     4381 gcaagggctg ggaagagtct ggggagggct ggggagaaga ggaggcctcg ctggagacct
     4441 cagaccatga gggcagccat gcccctcagc ccaggccccc taagacagag gaagatgagg
     4501 gtctacaggc agcgctaaca gtccctggtc ccaagctcct ggaaccctgt tcacccatcc
     4561 cgatcttgac agatgcccat gagctgcagc cccaagctga ggggatccag gaggctgggt
     4621 ggcagccgga agctgggact gaagcactgg gaagagtaga agatgagcca gagtttggtc
     4681 gtggggagat tcctgagggc ctccaggatt gggaggaggg cagagaagac agtgaggcag
     4741 atgagttagg ggaaactctc cctgactcta ctcccttggg cctctacctg aagtctcctg
     4801 cctccccaaa gtgggagcaa gctggagaac agaggctttt ccctcaaggg gaggccagga
     4861 aggaaggctg gagtcctgct gccctggctg cccagggtct cagtgaccca ccagaggaag
     4921 agcagcaagg ccatgactct gacctctcat ctgaggaatt tgaggaccta gggactgagg
     4981 cctctcttct tccaggggtt cccaaagagg tgtccgatca tctgggccaa gagccccccg
     5041 tactgcagcc tgcatgctgg gatcagggtg gggagtctga tgggtttgct gatgaggaag
     5101 agagtgggga agagggagag gaagaagatg ctgatgaaga agaaggagca gagtcaggga
     5161 ctcagtggtg ggggccaggg ccctctggtg gaggtgtcaa ggtccaggat gtcacccaga
     5221 gaggggacct ggaacatgaa tctgtgggtg acagtggcct ctgggatgat ggcttgagtg
     5281 gggctgcagc taatgttctt gtaactgccc tagagacggt gtctcaggac agtgctgagc
     5341 cttccgggtc agaggggtct gagtctgctt ccttggaggg ggaggaaggt caagcgattg
     5401 accatttaga tgccccccaa gaggtgacta gcgtggtccc aggggcagga gacacctttg
     5461 atatcagtgg ccagggcccc aacctggagt cagagcaagt gaatgggagg atggagaatg
     5521 gactagagca ggctgagggt caggtggttc tgcatgggga cgaggatcaa ggcatccctt
     5581 tacaggaaca gggtaccctc aaggcccctt tagtagggtc tcctgtgcat ctaggcccaa
     5641 gccagccgct gaagttcact ctgagtgggg tagatggaga ctcctggtcc tcaggggaag
     5701 attagaaact gcccctctgg cactgaggac ttagtggggg gtggggggaa tgtccctccc
     5761 tgctctgggc cagcactctt agctttgata acttgacctg tggtatctct cgtggagagg
     5821 tgtggctggc tgagacaggt gagatcctgc ctggatcacc ctgaaggctc aggtcagctg
     5881 agcctatagt tcaacgcccc ctttcttctg tggctcacct gctggaagag gcttgggccc
     5941 agagctttcc cacgagactg ctctggccag agcttgctag ccctgcctgt ctacagtagc
     6001 accacctgca cagggtctgg tgcatgccca gaggagcagc aatgatgagt gactctcatc
     6061 atctcagcct gctgagatct tgtttctctc ttcctccctt gaataaagct gtatccctac
     6121 ctacaaaaaa aaaaaaaaaa aaa
//
