LOCUS       NM_145972               5232 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus nucleolus and neural progenitor protein (Nepro),
            transcript variant 1, mRNA.
ACCESSION   NM_145972
VERSION     NM_145972.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5232)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 5232)
  AUTHORS   Hashimoto,M., Sato,T., Muroyama,Y., Fujimura,L., Hatano,M. and
            Saito,T.
  TITLE     Nepro is localized in the nucleolus and essential for
            preimplantation development in mice
  JOURNAL   Dev Growth Differ 57 (7), 529-538 (2015)
   PUBMED   26178919
  REMARK    GeneRIF: The Nepro is a nucleolus-associated protein, and its loss
            leads to the apoptosis before blastocyst formation in mice.
REFERENCE   5  (bases 1 to 5232)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 5232)
  AUTHORS   Tiberi,L., van den Ameele,J., Dimidschstein,J., Piccirilli,J.,
            Gall,D., Herpoel,A., Bilheu,A., Bonnefont,J., Iacovino,M., Kyba,M.,
            Bouschet,T. and Vanderhaeghen,P.
  TITLE     BCL6 controls neurogenesis through Sirt1-dependent epigenetic
            repression of selective Notch targets
  JOURNAL   Nat Neurosci 15 (12), 1627-1635 (2012)
   PUBMED   23160044
REFERENCE   7  (bases 1 to 5232)
  AUTHORS   Muroyama,Y. and Saito,T.
  TITLE     Identification of Nepro, a gene required for the maintenance of
            neocortex neural progenitor cells downstream of Notch
  JOURNAL   Development 136 (23), 3889-3893 (2009)
   PUBMED   19906856
REFERENCE   8  (bases 1 to 5232)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124180.5 and AC122217.4.
            
            On Feb 21, 2023 this sequence version replaced NM_145972.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.18607.1, AK144612.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               AC124180.5         193551-193737
            188-342             AC124180.5         196297-196451
            343-422             AC124180.5         198511-198590
            423-554             AC124180.5         198851-198982
            555-588             AC124180.5         199389-199422
            589-632             AC122217.4         198624-198667       c
            633-997             AC122217.4         197125-197489       c
            998-1059            AC122217.4         196702-196763       c
            1060-1316           AC122217.4         194024-194280       c
            1317-5232           AC122217.4         189190-193105       c
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 29.04 cM"
     gene            1..5232
                     /gene="Nepro"
                     /note="nucleolus and neural progenitor protein"
                     /db_xref="GeneID:212547"
                     /db_xref="MGI:MGI:2384836"
     exon            1..187
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1776
                     /gene="Nepro"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neural progenitor protein; protein nepro"
                     /codon_start=1
                     /product="nucleolus and neural progenitor protein isoform
                     1"
                     /protein_id="NP_666084.1"
                     /db_xref="CCDS:CCDS28187.1"
                     /db_xref="GeneID:212547"
                     /db_xref="MGI:MGI:2384836"
                     /translation="MAAAVRPGAEPWNRVRIPQAGNCSTLTVRDPSATLDICTAAVTK
                     GCHLVTQSLKSQTLDAEVDVLCSVLYSNHNRLGHHKPHLALRQVEQCLKRLKHMNLEG
                     SIEDLSQLLSANATQPGATENRVVPSQPVVEVVLMKVLGGCKLLLRLLDCCCKAFLLT
                     VKHLGLKEFIILNLVMVGLVSRLWVLHKGLLRRLISLYEPLLSLRQEISSIHPMPYFK
                     DFAFPSDITDFLGPSYLEVFKVKTPAASATKGVTKLLNKLFLMREQLPKMNEDTLDRL
                     SKPSEQMTSNPQSTVDLGQPVKACKRTRKEKPLGFDLRAFCTRLGNKATQETNRDFKY
                     SQSKLKTTKLPSQQLRTHWANDTVQRIRKTKTFAQLSEEIEMAIVWSRSKKLKTQATF
                     LGNKLLKSNRFRHVESQGYSLTKKLQCMKTSLCNCLLRGSRTSTSEHPPRQRRSKYKV
                     LSRQRKPQRKLQSTLLKETQQVPEGTLKNTRDSSAKRRCSGTVQRSDVCPNGKQVLRK
                     LAKPDLKTKVVVHGNLTGGSRNESGFQAKTQMHTHNAPDTAKEADDIDDIFALMGV"
     exon            188..342
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            343..422
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            423..554
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            555..632
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            633..997
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1059
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1316
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..5232
                     /gene="Nepro"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2733
                     /gene="Nepro"
                     /note="major polyA site"
     regulatory      4457..4462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nepro"
                     /note="hexamer: ATTAAA"
     polyA_site      4475
                     /gene="Nepro"
     regulatory      5069..5074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nepro"
                     /note="hexamer: AATAAA"
     polyA_site      5092
                     /gene="Nepro"
     regulatory      5197..5202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nepro"
                     /note="hexamer: ATTAAA"
     polyA_site      5232
                     /gene="Nepro"
ORIGIN      
        1 ccctccctcc ctctcctgcc caggccgctc aactcgctca cgctccgctc ggccttcccc
       61 ggccctccca cgccgctcga gatggccgct gcggtgcggc cgggtgcgga gccgtggaac
      121 cgcgtgagga tcccccaggc ggggaactgc agcacgctga ccgtgcggga ccccagcgcc
      181 accctggata tctgcactgc agctgtcact aaagggtgcc accttgtcac acagtcactc
      241 aagagccaga ccttggatgc ggaagtagat gtgttatgct cagttctcta cagcaatcac
      301 aacaggttgg gccaccacaa gccccatttg gccctcagac aggtagaaca gtgcttaaag
      361 cgtttgaaac atatgaattt ggagggctca attgaagacc tgtcacagtt gttgtctgcc
      421 aatgcaactc agcctggagc tacagaaaac cgcgtcgtcc ccagccagcc ggtggtggag
      481 gtggtgctga tgaaggtttt gggaggctgc aagttgttac tgcgcttgct ggactgctgc
      541 tgcaaggcat ttctgttgac tgtgaagcac ttagggctaa aggaattcat tattttgaac
      601 cttgtgatgg ttggactggt gagcaggcta tgggttctcc ataaaggact tttgagaagg
      661 ctcatttcct tatatgagcc attactcagc ttgcgtcagg agatctccag cattcatccc
      721 atgccttact tcaaagattt tgcctttccc tctgacatca ctgactttct aggcccctct
      781 taccttgaag tgttcaaggt aaaaacacct gcggcttctg ctactaaagg agtaacaaag
      841 ttgctaaata aactgttttt aatgagagag cagctaccaa agatgaatga agacacttta
      901 gatagacttt ccaaaccatc tgaacaaatg acaagcaatc cacagagcac tgtggacctg
      961 ggccagccag tgaaagcctg taagaggacc agaaaagaaa agccattggg atttgacctt
     1021 cgggcttttt gcacaagact tggaaacaaa gcgactcagg agaccaaccg tgactttaaa
     1081 tattctcagt ccaaactgaa gacaaccaaa cttccctctc agcaactgag aactcactgg
     1141 gctaacgata ctgtgcaaag aatccgaaag accaagacgt tcgcccaact ttctgaagaa
     1201 attgaaatgg caattgtgtg gtcccggagc aaaaagctca agactcaggc tacctttctg
     1261 ggcaacaaac ttcttaagag taataggttt agacatgtgg aatctcaagg ctatagtttg
     1321 acaaagaagc tccagtgcat gaaaacatct ctctgcaact gccttctccg aggctctcgt
     1381 accagcactt cagagcatcc tcccagacaa agaagatcaa agtataaagt tttatcaaga
     1441 caaaggaaac cgcagagaaa gttacagtca actcttttaa aggaaaccca gcaggtccct
     1501 gaagggactc tgaagaacac tagggacagc agtgctaaga ggagatgctc tgggacagtt
     1561 cagaggtcag atgtctgtcc taatggaaag caggtcttga gaaaacttgc aaagcctgac
     1621 ctaaaaacaa aggtggtggt ccacgggaat cttacagggg gcagtagaaa tgaatctggc
     1681 tttcaggcga agacacagat gcacacacac aatgcgccag acactgctaa agaggcagat
     1741 gacattgatg atatttttgc tttgatggga gtttagatgt actgacgtga aacttccgag
     1801 caggtaacaa gctggtccag tgctctgctc ctttaagggg tgttgctaag ctctgggaac
     1861 actgcttagc ctgagagctg cttctgtgca gtttgccaca ggagtgcatt caggtcaata
     1921 aacattcaca gcggccttgt gtaagctaat cacatggtga gtgtttggct tagactgtgc
     1981 aaggtgttaa ctatgatgaa cagtctgagg aacacgtcct tcatgactga aacaagtcag
     2041 tttctgaatt agatgatttt atgatctact gttgagttaa aatctgagta atttcataaa
     2101 atcttacata tctgaaggta aacagaacaa ggctggagta gcaaagcacc ataaagggtc
     2161 acctggcctc actgacctca ctccctttta aagttttata ttttgtgcga gggtgtgtac
     2221 gtatatgtgg atatcagagg gtaacttaca tgaatgtgaa tatcagtggg taacctgtga
     2281 gagttggttc tgtccaccta tgggttccag gagtggtgtc ttagttaggg ttttactgct
     2341 gtgaacaaac accatgacaa ggcaactctt ataaggacac catttaattg gggctgcctt
     2401 acaggttcag aggttcagtc cattatcgtc aaggtaggag gagcacggca gcatccaggc
     2461 aggcatggtg cagaaggagc tgagagttct acatcttgtt caaaagcaaa caggggaaaa
     2521 ctggcttcca ggcacctatg gtgccaattc ctgggctaag catattcaag tcaccacagg
     2581 tggacttcag attgtcaggc atagcatcat gtgtttaccc atgggctgag ccatctcctg
     2641 cccaggtctt ccttttgaat aacaaatgtg agtgtatgct atgttattaa agatggatta
     2701 tcaaaagatt ttccattact caaatgctta aaaccattat ctaatcttca ttttgtcact
     2761 gtgtaactcc taagggggta tatgaaaaag cttttggtgt tttcccattt ttgtttctat
     2821 gaaacatcac aaaaaagtag ctaagtgtat gccaggaata aggccaatca tgaatttgaa
     2881 acctggcatc aatgccatca cctgattatt tgtacataca tatctgtaag ctggaaatga
     2941 cagtcatgag ttacaaaatg agctgctcta cccatgaaga aaggtaatgt agtcatggaa
     3001 agtctgctca gccctgcagg gcaggaaggg gcaggagggg taggctaagg agaaaacagc
     3061 gagtcttcct tccccctgcc ctccagtgac ggcacagcct acggcctgca agcctgaaac
     3121 cttaggcaca actgcacact tgctttaaag gcccgagggt gggagtggca ggatatgaat
     3181 ctggattttg cacactcact tcaactagga cactgctgct ttctgctata aaactgacca
     3241 tactcatgag gctcaagaaa gctccaagtt tgaatatttc tgattttggg attcttggcc
     3301 gtggggcatt ctacaattaa agactgcaaa cagccccaaa ttctgtaatc tgaaccattt
     3361 ccagttccta gctttgcaga taagagctga aaggagcatt aggtgttcca gttgtaagtg
     3421 cagagtatga cagtttgtga cctttcaaat cctcttgacc ccactgtgtc aaccccctgt
     3481 tgcatagggc tggaccttgc cttctgctct gatgacctgt cctggcattt tagaatgtgt
     3541 ggtctgtgtg tatagtatac gtgtctgtat acatgtatga gcgttatgaa tctctgggtg
     3601 cttgggtatg tacaactggg aagaggtgtg tccctgcctc atcctctggt ccctgggcag
     3661 gcattgcact ggaagcccat gccttgagaa tgctcagaaa gcccctcaag agcaagccgc
     3721 agtgcctgtc tgtgtggtgg cccagcatgg tgggcaagca gtccttccac ttcagcgctc
     3781 cttttcctgg tcacctcagc tgtctgctgc tgtgggaagc atcgacagac agttctgctt
     3841 ttggcagaag gatggctttc aaagtaatta ttttgacatc tatgttttgc tttgaaaaac
     3901 aaaacaaaac ctttctgcta gttagggtaa aatagactaa ttctatcaac tgtagaacaa
     3961 tggaaacatt tacaattggg tttttaaaaa gccacagagt tgtggagcct taccacagct
     4021 ataacctctg acagcagtgt cactttggaa tgctaagata tctcatgaag agttatgagc
     4081 tgccagtcaa agctcgtctc cccattcaga atctagcaca gtgactctaa acactgcact
     4141 gactgggaac ttctccacct aaatctaact cctgtttact tttgctgcga ctttcaaggt
     4201 ggagggcctt ctatgataaa gcctgtgact tctgctgtgc tgtgtggttg ctttgggttt
     4261 gtaaacatgc agctgtcttc ggtattataa cttgttagct ttgttatctt tcatagctaa
     4321 ggtgccgata gtcaccgtgg aggtgaggca acatcaagta gttttcccaa ggacactaga
     4381 agcagaacct gaagatcaag tctcaggact ggtgcttcct tggtctggca agttttgaca
     4441 atttttttca tattcaatta aatactagaa gaagagttat tagtatacaa tcatttcctt
     4501 tctattcaca tatacacgct ttacagaggg cactcccctc ctttacctag ttcacgttta
     4561 cagaattgaa gacagtagac atttataaga gaaaaacctg acttatccaa cctctttata
     4621 gaagcagggt ctcacttcat gcacttgtat atgctctgga cttgtcagtt aaaatagaaa
     4681 agccagctag catctgtcct aatgaccaat atgatttaat tcattatttg taacccttac
     4741 ccccagcgca tttgttcttg cttcagggta atgcaggtct taatgccgaa tctccttcaa
     4801 gttgagatgc acaatattta aaacaatatt ggcattcttc taggtgttta actccagttg
     4861 tcaggaagaa ctctgacaca tcatctactc tgggagcatt tacttaagac ttcctgttat
     4921 atctgcgata aagcatttca acagatggac tccagaattg gtcactgcac tgtgaaaaga
     4981 aaagaaaact tgattttctt ctttacctca gaggttgcaa cagacctcaa ttcaagtaat
     5041 acaaagcgca aaaggttttt tttcttcaaa taaactcttt taagtttttc gagacaaggt
     5101 ttctctgtgt agccctggct gtcctggatc tcactctgta gaccaggctg gcctcgaact
     5161 cagaaatccg cctgcctctg cctcctgagt gctgggatta aaggcgtgtg ccaccacaca
     5221 cagctgtgtc ga
//
