LOCUS       NM_025684               1829 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus nephrocan (Nepn), mRNA.
ACCESSION   NM_025684
VERSION     NM_025684.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Wang,Z., Fan,R., Russo,A., Cernilogar,F.M., Nuber,A., Schirge,S.,
            Shcherbakova,I., Dzhilyanova,I., Ugur,E., Anton,T., Richter,L.,
            Leonhardt,H., Lickert,H. and Schotta,G.
  TITLE     Dominant role of DNA methylation over H3K9me3 for IAP silencing in
            endoderm
  JOURNAL   Nat Commun 13 (1), 5447 (2022)
   PUBMED   36123357
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1829)
  AUTHORS   Chen,X., Liu,F., Li,B., Wang,Y., Yuan,L., Yin,A., Chen,Q., Hu,W.,
            Yao,Y., Zhang,M., Wu,Y. and Chen,K.
  TITLE     Neuropathy-associated Fars2 deficiency affects neuronal development
            and potentiates neuronal apoptosis by impairing mitochondrial
            function
  JOURNAL   Cell Biosci 12 (1), 103 (2022)
   PUBMED   35794642
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1829)
  AUTHORS   Montano,E., Pollice,A., Lucci,V., Falco,G., Affinito,O., La
            Mantia,G., Vivo,M. and Angrisano,T.
  TITLE     Pancreatic Progenitor Commitment Is Marked by an Increase in
            Ink4a/Arf Expression
  JOURNAL   Biomolecules 11 (8), 1124 (2021)
   PUBMED   34439790
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1829)
  AUTHORS   Addeo,M., Buonaiuto,S., Guerriero,I., Amendola,E., Visconte,F.,
            Marino,A., De Angelis,M.T., Russo,F., Roberto,L., Marotta,P.,
            Russo,N.A., Iervolino,A., Amodio,F., De Felice,M., Lucci,V. and
            Falco,G.
  TITLE     Insight into Nephrocan Function in Mouse Endoderm Patterning
  JOURNAL   Int J Mol Sci 21 (1), 8 (2019)
   PUBMED   31861348
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1829)
  AUTHORS   Li,X., Udager,A.M., Hu,C., Qiao,X.T., Richards,N. and Gumucio,D.L.
  TITLE     Dynamic patterning at the pylorus: formation of an epithelial
            intestine-stomach boundary in late fetal life
  JOURNAL   Dev Dyn 238 (12), 3205-3217 (2009)
   PUBMED   19877272
REFERENCE   7  (bases 1 to 1829)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   8  (bases 1 to 1829)
  AUTHORS   Hou,J., Charters,A.M., Lee,S.C., Zhao,Y., Wu,M.K., Jones,S.J.,
            Marra,M.A. and Hoodless,P.A.
  TITLE     A systematic screen for genes expressed in definitive endoderm by
            Serial Analysis of Gene Expression (SAGE)
  JOURNAL   BMC Dev Biol 7, 92 (2007)
   PUBMED   17683524
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1829)
  AUTHORS   Sherwood,R.I., Jitianu,C., Cleaver,O., Shaywitz,D.A., Lamenzo,J.O.,
            Chen,A.E., Golub,T.R. and Melton,D.A.
  TITLE     Prospective isolation and global gene expression analysis of
            definitive and visceral endoderm
  JOURNAL   Dev Biol 304 (2), 541-555 (2007)
   PUBMED   17328885
REFERENCE   10 (bases 1 to 1829)
  AUTHORS   Mochida,Y., Parisuthiman,D., Kaku,M., Hanai,J., Sukhatme,V.P. and
            Yamauchi,M.
  TITLE     Nephrocan, a novel member of the small leucine-rich repeat protein
            family, is an inhibitor of transforming growth factor-beta
            signaling
  JOURNAL   J Biol Chem 281 (47), 36044-36051 (2006)
   PUBMED   16990280
  REMARK    GeneRIF: NPN is a novel member of the SLRP family that may play
            important roles in kidney development and pathophysiology by
            functioning as an endogenous inhibitor of TGF-beta signaling
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153526.13.
            
            On Dec 16, 2022 this sequence version replaced NM_025684.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017656.1, AK017780.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               AC153526.13        73072-73291
            221-1122            AC153526.13        81822-82723
            1123-1829           AC153526.13        85368-86074
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 26.6 cM"
     gene            1..1829
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /note="nephrocan"
                     /db_xref="GeneID:66650"
                     /db_xref="MGI:MGI:1913900"
     exon            1..220
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1570
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /codon_start=1
                     /product="nephrocan precursor"
                     /protein_id="NP_079960.1"
                     /db_xref="CCDS:CCDS23840.1"
                     /db_xref="GeneID:66650"
                     /db_xref="MGI:MGI:1913900"
                     /translation="MHPLWAFLLGLSLTNGLSANCPGRCSCDSMQSVQCYRLMELPSG
                     IPSTTKRLYISHSRIQHLQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLEL
                     WKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSML
                     SPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFS
                     GNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQL
                     TNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVV
                     SLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQ
                     LQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGG
                     QCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDD
                     YEID"
     exon            221..1122
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1829
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1807..1812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /note="hexamer: AATAAA"
     polyA_site      1829
                     /gene="Nepn"
                     /gene_synonym="5730521E12Rik; Npn; periolin"
                     /note="major polyA site"
ORIGIN      
        1 gcttgctact tgcaaacctc tgtgttggac aatgcacccg ctttgggctt ttcttttggg
       61 gctctctctt accaatggcc tcagtgcaaa ctgtccagga cgatgcagtt gtgattctat
      121 gcagtctgta caatgctaca ggctcatgga actcccatca ggcattcctt ccaccaccaa
      181 gagactctac attagccaca gcagaatcca gcaccttcag ctatctaact tcactggact
      241 gttggctctg gaagatttta ttctgctggc cagtggaaca gagtctattg aaaatgacac
      301 cttcaaaact ctgagtacac taaagactct agaactttgg aaaaataaat tgagacaagt
      361 ccccagtgct ctcccagcca accttgaggt gttaaaatta aatgataatg ccatatgtgc
      421 tctacgtgga tcagagtttg aaggactgaa gaacttgaaa gtccttgaac ttaaaaacaa
      481 cttgatctcg tccctgtctc ccagcatgct ctccccactt gccagtttgc agagtttgat
      541 ggtggatggt aacaacatag agtccgtggt tggacctctg agccttccgc atctcaagta
      601 catgagcatg gagaacaacc aactccacct tataccaggg aatgtcttca cttccctaca
      661 gaatttgcag ttcctcagtt tcagtggtaa ttttctaact aaaattccca taaatctacc
      721 caaatccttg ctatctttaa agatggagag aaaccagctc aaagtggtca ggtttcgcga
      781 tatgaaacac ttggagaacc tgtcccatct ttacctgtca gagaacttcc tgtcttccat
      841 tgatggggca cagcagctga ccaatttaac aactctggag gtttcccaaa accagcttca
      901 aatgttgcct cccaggctac catcgaggtt acagaagctt gattgtagca gtaacttcat
      961 tcagagagtg acagcaccag aattccagga cctccgagac ctgaaacatc tgtttctaga
     1021 caacaatgtt gtcagcttgt tcgaggctgg ggcccttcag aggtgttctc agctgtccaa
     1081 cctggccctg gagcagaatc tgctgttgtc tatccctctg aggctcccta agaccctggc
     1141 caggctggac ctcaagggca atgccatcca ggacatggct gaaagggagc tcagggacct
     1201 caaacaactg caggtcctca acctcaggaa caaccggatc tcggccttag atttcaaggc
     1261 actggagggg ctgcctcggc tcaggcatct ctacctggat gggaatccct ggaactgcac
     1321 ttgcagtctc ctacgagcca gggaagtcct gaaggccaag ggcacggacg tgaaaggggg
     1381 acagtgcgca gccccagcag agcgacaggg ggagagctgg atgtcctcca agaagatcct
     1441 gaggcaatgt gagcatcact tacagcagag tgagaaaagc aaagagacca agaagaaacc
     1501 caaacccgaa gactcttcca gcatcagact caacatggat gacgacgatg acgattatga
     1561 aatagattag agatgagctt tgtttccagt ttagaaaatg atttttaatg tccactgtct
     1621 taagcacaag tgtgctatca tttaaacatt gcaagccttc tttgtaagtt gttcttgcta
     1681 cttttgaaag aaaaaaaaat aagggttaag gattagttaa aatctgtgag gaaacaaaag
     1741 taggcttctt tcctggtttg aaacaagttg tgttttagtc tgaaacttag gcaaataaca
     1801 atgtcaaata aaattatcat ttaaatcaa
//
