LOCUS       NM_001142647            3423 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nuclear envelope integral membrane protein 2 (Nemp2),
            transcript variant 1, mRNA.
ACCESSION   NM_001142647
VERSION     NM_001142647.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Harrow,J.L., Steward,C.A., Frankish,A., Gilbert,J.G.,
            Gonzalez,J.M., Loveland,J.E., Mudge,J., Sheppard,D., Thomas,M.,
            Trevanion,S. and Wilming,L.G.
  TITLE     The Vertebrate Genome Annotation browser 10 years on
  JOURNAL   Nucleic Acids Res 42 (Database issue), D771-D779 (2014)
   PUBMED   24316575
REFERENCE   3  (bases 1 to 3423)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB848894.1, CD553439.1, AI153571.1 and BC094370.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849380,
                              SAMN00849382 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BB848894.1         4-75
            73-599              CD553439.1         1-527
            600-1031            AI153571.1         49-480
            1032-3423           BC094370.1         1976-4367
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 26.99 cM"
     gene            1..3423
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /note="nuclear envelope integral membrane protein 2"
                     /db_xref="GeneID:227094"
                     /db_xref="MGI:MGI:2444113"
     exon            1..147
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            148..263
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            264..495
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            496..568
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            569..662
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            663..777
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            778..1003
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1180
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..3394
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3370..3375
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /note="hexamer: ATTAAA"
     polyA_site      3394
                     /gene="Nemp2"
                     /gene_synonym="5330401P04Rik; 9830163O08; Tmem194b"
                     /note="major polyA site"
ORIGIN      
        1 cctccgcttt cccgctcttg gtccgctgcg ccacttccgc cgctctggcc gcgatgctgc
       61 ctaggttgtg gtggctggtg ctctggctgc agcccctggc tacactgcca gcgagcgcgg
      121 tgcacgacga ggaggcagcg atgtcagttc ccagatgtaa atctctgaag gaaacggact
      181 taattaaaac ttctgtgtca gactgctatt gctacaatca acattcgcaa atccagtgga
      241 catatatgtg gtcaactgtg caggtgacag taaccagccc gggcctgctc aatattgtat
      301 atatcacagg aagtcataac tgccagcaca cagaaagcat tttatcattt atcaaatgtg
      361 tgactcataa cttttgggca ccagaggagg ctgaggagat aaccatagtc ttcagtccat
      421 atggagaaac tgtgtgcttc tctgtgaagc ctgttgggag gctccttccc tacatagtaa
      481 gcgtgagtcg aaacattgtg gatttcaaac tcttccttgt gtttgtgaca ggcattttcc
      541 ttttcttgta tgcaaagacc ttgagccaaa gccctgtttt ctattactct tccggtaccg
      601 tgttaggtat tctaatgaca ttagtctttg tccttctgat ggctaagaaa cacattccaa
      661 agtatagcac atttggggcg ctaatgattg gttgttggtt tgcttcagtt tatgttttgt
      721 gccagttgat ggaagatctg aagtggctgt ggtatggaaa cagaatgtat atattaggct
      781 atgtggtggt tgttggactt tgtagctttg ctgcctgtta cagtcacggg cctcttgctg
      841 atgaggggag cagagacctt ctcatgtgga ccctgaggct cttctccctg gctctggtct
      901 acactggtgt ggccgctcct cagtttgcct atgctgttct gatcgtcctc ttgttctcct
      961 ggagtctgca ctatctcctg agagcattca gctatctcag gtggaaaatg aggccgtggt
     1021 tcacagcaga acctcaggtg gcgaggtatc tcactgatga cgagtacagg gagcaagctg
     1081 aagctgcaac agccagggct ctcgaggaac tgcgccaagc ctgctgcagg cctgacttcc
     1141 cttcctggct ggctgtctcc agactccagg ctcctaaaaa gtttgcagag tttgttcttg
     1201 gagccagcca cctgtcacca gaggaagtca gcacacatga aaagcagtat ggccttgggg
     1261 gtgccttcct ggaggaacag ctctttagcc tgcagactga cagtctacct gcaagttgac
     1321 ttcattctga acaggaatgc aaataaaagg caaagatcct actccagctg ggtgggaccc
     1381 atgcacctca gagaaaaata agccatgcaa ataaggaaaa actcgttttc atttgtgcaa
     1441 gagagcttta ctgtggtggt gtgaaggctg aaagtgtcat tgccacatgc cctgaatgaa
     1501 gaccttcatg gaaacagtgc aaacctgttc tttaggacca gtaaacagaa cctgagacct
     1561 tcccttcacc aggcactaac tcccttctgg atgcccagga gctggctcaa aaggctgcat
     1621 tattgccctc cgtccttccc ctcccccata ccagttcacc agtatcagtg ctttctaagg
     1681 tgctattcac gtgatagtac gaagtgctgc tgaaagaatt ctgatgctgg ctgtggttaa
     1741 ctcagagtca tatattctgc aagctcctcg gtctttcatg ttacaaagtc attgtgggtc
     1801 tcctaaaaag cagacatgca cagggcttct aagttactaa ccacaaacca tttttcatga
     1861 gactaacaat ctttagaact catgtttgga aatgctattg atggtggaat ttaggtttga
     1921 gagttagagc aaaagtgtcg ttagcagcct tggcagcccc tggtgcatct gggactcagg
     1981 cataagacac ttcttcatgt gcaggttcag ggctgtctcg ctctagcctt ggtctcatgg
     2041 tgttggtgcc ccaagttttg ttgttgtttt gttttaataa ctgtgattaa aaagaaacat
     2101 ttcttaggct tatatttaat gttttgataa gattttatat gcagacagat taaaatatta
     2161 taatttttgt actttttata tattgaaatt gaaagtatga aagcatttgt gacattttgt
     2221 gatttatttt ttaaaactga ttatatatgt ttacaatgag ttcccacaaa atagatttgt
     2281 gatacctggt tttttttttt gttttgtttt cttttgtttt tttttagtga gagcaaatgt
     2341 ttgtttcttg atccatgata aacttcaggt taaaatgagc tacaaattgg gctttgggcc
     2401 caaggaaagg agcagaaata gccttaagag ttggctatgg cagacattct gtgaacaggt
     2461 cgctacttga tcctctgaga atcaaagtca gggtgtttta tcttatgtac tgtaatgatt
     2521 aaacaacaac agaaacacaa agcatgagag aggaggaggg ggacaagaca cggacttggt
     2581 cactgtcatc ccctgagagt gatgctgact tcggaacttt ctatattcac tctcaaatga
     2641 tgctctctct ttgacgctct atgtcagtgt ttctgcctct gccttttgac agacataggt
     2701 aatatcagga gtctttttcc ttttcttaga aacctcactc aggtcaatct gtgcctttac
     2761 gtgttgactt taaaacgtca aagaataggg tctggagaga tggcttagtg gctaaaaaga
     2821 gccttacaga ggatttgagt ttcgtcctca gcatctgagt ggcatggcag cttgccgcca
     2881 tccataactc tagtcccagg gagcctggta ccttcctctg agtttgcatg cacatacaca
     2941 caggcatgaa cacttgtgtg taaacaatgt gcttctagtc attttggcaa ctattttgca
     3001 aaaatagaca ggagttgtaa cattttgcca cagcttaatc tggttttctt tttcagtagc
     3061 tttatttctg ggcattgtaa caggtcaggt gtgtcagtac ttgggcattg ccatgttcac
     3121 ctctcactac acagcttgcc tagtatagaa tgtgtttgtg tgtgtctggc acatcagcac
     3181 cccctgagcc accagaacaa ccccacagtt gggacttcac tgtgaagctg tagctcccag
     3241 caccagcaag cccgtgcacc cacaatgaaa acagtggtcg tcttttgaga gagctgtttt
     3301 tcatgttcat ttctggaaag aatcatggat ctgtcagatt gctatgtgag agctgtgaga
     3361 agctaaacaa ttaaaacatt tctggtgaaa tgttaaaaaa aaaaaaaaaa aaaaaaaaaa
     3421 aaa
//
