LOCUS       NP_543125                698 aa            linear   ROD 12-JUN-2025
DEFINITION  serine/threonine-protein kinase Nek8 [Mus musculus].
ACCESSION   NP_543125
VERSION     NP_543125.1
DBSOURCE    REFSEQ: accession NM_080849.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 698)
  AUTHORS   Cheong,A., Craciun,F., Husson,H., Gans,J., Escobedo,J., Chang,Y.C.,
            Guo,L., Goncalves,M., Kaplan,N., Smith,L.A., Moreno,S.,
            Boulanger,J., Liu,S., Saleh,J., Zhang,M., Blazier,A.S., Qiu,W.,
            Macklin,A., Iyyanki,T., Chatelain,C., Khader,S., Natoli,T.A.,
            Ibraghimov-Beskrovnaya,O., Ofengeim,D. and Proto,J.D.
  TITLE     Glucosylceramide synthase modulation ameliorates murine renal
            pathologies and promotes macrophage effector function in vitro
  JOURNAL   Commun Biol 7 (1), 932 (2024)
   PUBMED   39095617
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 698)
  AUTHORS   Djaziri,N., Burel,C., Abbad,L., Bakey,Z., Piedagnel,R. and
            Lelongt,B.
  TITLE     Cleavage of periostin by MMP9 protects mice from kidney cystic
            disease
  JOURNAL   PLoS One 18 (12), e0294922 (2023)
   PUBMED   38039285
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 698)
  AUTHORS   Claus,L.R., Chen,C., Stallworth,J., Turner,J.L., Slaats,G.G.,
            Hawks,A.L., Mabillard,H., Senum,S.R., Srikanth,S.,
            Flanagan-Steet,H., Louie,R.J., Silver,J., Lerner-Ellis,J.,
            Morel,C., Mighton,C., Sleutels,F., van Slegtenhorst,M., van Ham,T.,
            Brooks,A.S., Dorresteijn,E.M., Barakat,T.S., Dahan,K., Demoulin,N.,
            Goffin,E.J., Olinger,E., Larsen,M., Hertz,J.M., Lilien,M.R.,
            Obeidova,L., Seeman,T., Stone,H.K., Kerecuk,L., Gurgu,M., Yousef
            Yengej,F.A., Ammerlaan,C.M.E., Rookmaaker,M.B., Hanna,C.,
            Rogers,R.C., Duran,K., Peters,E., Sayer,J.A., van Haaften,G.,
            Harris,P.C., Ling,K., Mason,J.M., van Eerde,A.M. and Steet,R.
  CONSRTM   Genomics England Research Consortium
  TITLE     Certain heterozygous variants in the kinase domain of the
            serine/threonine kinase NEK8 can cause an autosomal dominant form
            of polycystic kidney disease
  JOURNAL   Kidney Int 104 (5), 995-1007 (2023)
   PUBMED   37598857
  REMARK    GeneRIF: Certain heterozygous variants in the kinase domain of the
            serine/threonine kinase NEK8 can cause an autosomal dominant form
            of polycystic kidney disease.
REFERENCE   4  (residues 1 to 698)
  AUTHORS   Swenson-Fields,K.I., Ward,C.J., Lopez,M.E., Fross,S., Heimes
            Dillon,A.L., Meisenheimer,J.D., Rabbani,A.J., Wedlock,E.,
            Basu,M.K., Jansson,K.P., Rowe,P.S., Stubbs,J.R., Wallace,D.P.,
            Vitek,M.P. and Fields,T.A.
  TITLE     Caspase-1 and the inflammasome promote polycystic kidney disease
            progression
  JOURNAL   Front Mol Biosci 9, 971219 (2022)
   PUBMED   36523654
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 698)
  AUTHORS   Zhang,J.Q.J., Burgess,J., Stepanova,D., Saravanabavan,S.,
            Wong,A.T.Y., Kaldis,P. and Rangan,G.K.
  TITLE     Role of cyclin-dependent kinase 2 in the progression of mouse
            juvenile cystic kidney disease
  JOURNAL   Lab Invest 100 (5), 696-711 (2020)
   PUBMED   31915367
REFERENCE   6  (residues 1 to 698)
  AUTHORS   Kuida,S. and Beier,D.R.
  TITLE     Genetic localization of interacting modifiers affecting severity in
            a murine model of polycystic kidney disease
  JOURNAL   Genome Res 10 (1), 49-54 (2000)
   PUBMED   10645949
REFERENCE   7  (residues 1 to 698)
  AUTHORS   Iakoubova,O., Dushkin,H., Pacella,L. and Beier,D.R.
  TITLE     Genetic analysis of modifying loci on mouse chromosome 1 that
            affect disease severity in a model of recessive PKD
  JOURNAL   Physiol Genomics 1 (2), 101-105 (1999)
   PUBMED   11015567
  REMARK    Publication Status: Online-Only
REFERENCE   8  (residues 1 to 698)
  AUTHORS   Iakoubova,O.A., Dushkin,H. and Beier,D.R.
  TITLE     Genetic analysis of a quantitative trait in a mouse model of
            polycystic kidney disease
  JOURNAL   Am J Respir Crit Care Med 156 (4 Pt 2), S72-S77 (1997)
   PUBMED   9351583
REFERENCE   9  (residues 1 to 698)
  AUTHORS   Iakoubova,O.A., Dushkin,H. and Beier,D.R.
  TITLE     Localization of a murine recessive polycystic kidney disease
            mutation and modifying loci that affect disease severity
  JOURNAL   Genomics 26 (1), 107-114 (1995)
   PUBMED   7782068
REFERENCE   10 (residues 1 to 698)
  AUTHORS   Atala,A., Freeman,M.R., Mandell,J. and Beier,D.R.
  TITLE     Juvenile cystic kidneys (jck): a new mouse mutation which causes
            polycystic kidneys
  JOURNAL   Kidney Int 43 (5), 1081-1085 (1993)
   PUBMED   8510385
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB646522.1, AF407579.1 and
            BC070457.1.
            
            Summary: This gene encodes a NIMA-related kinase. Members of this
            serine/threonine protein kinase family are structurally-related to
            NIMA (never in mitosis, gene A) which controls mitotic signaling in
            Aspergillus nidulans. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070457.1, AK154358.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     Protein         1..698
                     /product="serine/threonine-protein kinase Nek8"
                     /EC_number="2.7.11.1"
                     /note="nimA-related protein kinase 8; never in mitosis
                     A-related kinase 8; juvenile cystic kidney; NIMA-related
                     expressed kinase 8"
                     /calculated_mol_wt=75134
     Region          3..258
                     /region_name="STKc_Nek8"
                     /note="Catalytic domain of the Protein Serine/Threonine
                     Kinase, Never In Mitosis gene A (NIMA)-related kinase 8;
                     cd08220"
                     /db_xref="CDD:270859"
     Site            order(10..14,18,31,33,64,80..83,87,89,128,130,132..133,
                     135,147,150,152,165..168)
                     /site_type="active"
                     /db_xref="CDD:270859"
     Site            order(10..11,13..14,18,31,33,64,81..83,87,133,135,152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270859"
     Site            order(14,87,89,128,130,132,150,165..168)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270859"
     Site            146..168
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270859"
     Site            162
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by autocatalysis.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          278..309
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          319..653
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          416..467
                     /region_name="RCC1 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          468..519
                     /region_name="RCC1 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          520..585
                     /region_name="RCC1 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          586..637
                     /region_name="RCC1 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     Region          637..679
                     /region_name="RCC1"
                     /note="Regulator of chromosome condensation (RCC1) repeat;
                     pfam00415"
                     /db_xref="CDD:395335"
     Region          638..690
                     /region_name="RCC1 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91ZR4.1)"
     CDS             1..698
                     /gene="Nek8"
                     /gene_synonym="4632401F23Rik; b2b1449Clo; jck"
                     /coded_by="NM_080849.3:61..2157"
                     /db_xref="CCDS:CCDS25091.1"
                     /db_xref="GeneID:140859"
                     /db_xref="MGI:MGI:1890646"
ORIGIN      
        1 mekyerirvv grgafgivhl clrkadqklv ilkqipveqm tkeerqaaqn ecqvlkllnh
       61 pnvieyyenf ledkalmiam eyapggtlae fiqkrcnsll eeetilhffv qillalhhvh
      121 thlilhrdlk tqnilldkhr mvvkigdfgi skilsskska ytvvgtpcyi spelcegkpy
      181 nqksdiwalg cvlyelaslk rafeaanlpa lvlkimsgtf apisdryspe lrqlvlslls
      241 lepaqrppls himaqplcir allnihtdvg svrmrraeks ltpgppiasg stgsratsar
      301 crgvprgpvr paippplssv yawggglssp lrlpmlntev vqvaagrtqk agvtrsgrli
      361 lweapplgag ggtllpgave lpqpqfvsrf legqsgvtik hvacgdlfta cltdrgiimt
      421 fgsgsngclg hgnltdisqp tiveallgye mvqvacgash vlalstdgel fawgrgdggr
      481 lglgtreshn cpqqvpvapg qeaqrvvcgi dssmiltspg rvlacgsnrf nklgldhlsl
      541 deepvpyqqv eealsftplg sapldqepll cvdlgtahsa aitasgdcyt fgsnqhgqlg
      601 tssrrvsrap crvqglegik mvmvacgdaf tvavgaegev yswgkgtrgr lgrrdedagl
      661 prpvqldeth pymvtsvscc hgntllavrs vtdepvpp
//
