LOCUS       NM_021606               3168 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus NIMA (never in mitosis gene a)-related expressed
            kinase 6 (Nek6), transcript variant 2, mRNA.
ACCESSION   NM_021606
VERSION     NM_021606.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Shao,S., Xiao,L., Jia,M., Zhang,C., Zhao,G., Yao,R., Wang,X. and
            Gao,L.
  TITLE     Never in mitosis gene A-related kinase-6 deficiency deteriorates
            diabetic cardiomyopathy via regulating heat shock protein 72
  JOURNAL   J Mol Med (Berl) 101 (4), 419-430 (2023)
   PUBMED   36867206
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Jeltema,D., Wang,J., Cai,J., Kelley,N., Yang,Z. and He,Y.
  TITLE     A Single Amino Acid Residue Defines the Difference in NLRP3
            Inflammasome Activation between NEK7 and NEK6
  JOURNAL   J Immunol 208 (8), 2029-2036 (2022)
   PUBMED   35354613
  REMARK    GeneRIF: A Single Amino Acid Residue Defines the Difference in
            NLRP3 Inflammasome Activation between NEK7 and NEK6.
REFERENCE   3  (bases 1 to 3168)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3168)
  AUTHORS   Fu,B., Xue,W., Zhang,H., Zhang,R., Feldman,K., Zhao,Q., Zhang,S.,
            Shi,L., Pavani,K.C., Nian,W., Lin,X. and Wu,H.
  TITLE     MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium
            tuberculosis through Targeting LNX1 via NEK6 Accumulation to
            Promote Anti-Apoptotic STAT3 Signaling
  JOURNAL   mBio 11 (3), e00557-20 (2020)
   PUBMED   32487755
  REMARK    GeneRIF: MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium
            tuberculosis through Targeting LNX1 via NEK6 Accumulation to
            Promote Anti-Apoptotic STAT3 Signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3168)
  AUTHORS   Shi,H., Wang,Y., Li,X., Zhan,X., Tang,M., Fina,M., Su,L., Pratt,D.,
            Bu,C.H., Hildebrand,S., Lyon,S., Scott,L., Quan,J., Sun,Q.,
            Russell,J., Arnett,S., Jurek,P., Chen,D., Kravchenko,V.V.,
            Mathison,J.C., Moresco,E.M., Monson,N.L., Ulevitch,R.J. and
            Beutler,B.
  TITLE     NLRP3 activation and mitosis are mutually exclusive events
            coordinated by NEK7, a new inflammasome component
  JOURNAL   Nat Immunol 17 (3), 250-258 (2016)
   PUBMED   26642356
REFERENCE   6  (bases 1 to 3168)
  AUTHORS   Kaput,J., Klein,K.G., Reyes,E.J., Kibbe,W.A., Cooney,C.A.,
            Jovanovic,B., Visek,W.J. and Wolff,G.L.
  TITLE     Identification of genes contributing to the obese yellow Avy
            phenotype: caloric restriction, genotype, diet x genotype
            interactions
  JOURNAL   Physiol Genomics 18 (3), 316-324 (2004)
   PUBMED   15306695
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3168)
  AUTHORS   Hashimoto,Y., Akita,H., Hibino,M., Kohri,K. and Nakanishi,M.
  TITLE     Identification and characterization of Nek6 protein kinase, a
            potential human homolog of NIMA histone H3 kinase
  JOURNAL   Biochem Biophys Res Commun 293 (2), 753-758 (2002)
   PUBMED   12054534
REFERENCE   8  (bases 1 to 3168)
  AUTHORS   Feige,E. and Motro,B.
  TITLE     The related murine kinases, Nek6 and Nek7, display distinct
            patterns of expression
  JOURNAL   Mech Dev 110 (1-2), 219-223 (2002)
   PUBMED   11744387
  REMARK    GeneRIF: During early embryogenesis nek6 is highly expressed in
            primary giant trophoblast cells. Later in embryogenesis, nek6 is
            found in ventricular and sub-ventricular regions.
REFERENCE   9  (bases 1 to 3168)
  AUTHORS   Kimura,M. and Okano,Y.
  TITLE     Identification and assignment of the human NIMA-related protein
            kinase 7 gene (NEK7) to human chromosome 1q31.3
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 33-38 (2001)
   PUBMED   11701951
REFERENCE   10 (bases 1 to 3168)
  AUTHORS   Kandli,M., Feige,E., Chen,A., Kilfin,G. and Motro,B.
  TITLE     Isolation and characterization of two evolutionarily conserved
            murine kinases (Nek6 and nek7) related to the fungal mitotic
            regulator, NIMA
  JOURNAL   Genomics 68 (2), 187-196 (2000)
   PUBMED   10964517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF218847.1 and AK086700.1.
            
            On Apr 25, 2009 this sequence version replaced NM_021606.2.
            
            Transcript Variant: This variant (2) represents use of an alternate
            promoter, compared to variant 1. Both variants 1 and 2 encode the
            same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK089919.1, AK086700.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AF218847.1         1-3
            4-3168              AK086700.1         9-3173
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 24.41 cM"
     gene            1..3168
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="NIMA (never in mitosis gene a)-related expressed
                     kinase 6"
                     /db_xref="GeneID:59126"
                     /db_xref="MGI:MGI:1891638"
     exon            1..97
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            98..216
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1068
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /EC_number="2.7.11.34"
                     /note="nimA-related protein kinase 6; never in mitosis
                     A-related kinase 6; NIMA-related expressed kinase 6"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek6"
                     /protein_id="NP_067619.1"
                     /db_xref="CCDS:CCDS16009.1"
                     /db_xref="GeneID:59126"
                     /db_xref="MGI:MGI:1891638"
                     /translation="MAGQPSHMPHGGSPNHLCHALGPAPPPDPQRLPNTLSFRCSLAD
                     FQIEKKIGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQLNH
                     PNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAV
                     EHMHSRRVMHRDIKPANVFITATGIVKLGDLGLGRFFSSETTAAHSLVGTPYYMSPER
                     IHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSE
                     KLRELVSMCIYPDPDHRPDIVYVHQVARQMHVWTSST"
     exon            217..357
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            358..420
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            421..531
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            532..640
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            641..748
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            749..843
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            844..957
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            958..3168
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1491..1496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="hexamer: AATAAT"
     polyA_site      1509
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="major polyA site"
     regulatory      2141..2146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="hexamer: AATACA"
     polyA_site      2167
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
     regulatory      2225..2230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="hexamer: TATAAA"
     polyA_site      2251
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="major polyA site"
     regulatory      2875..2880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2898
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
     regulatory      3141..3146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3168
                     /gene="Nek6"
                     /gene_synonym="1300007C09Rik"
ORIGIN      
        1 agcggcggct gaaccaagcg gactagctgc ggccgcagcg ccgccaactc gcgtgtgacc
       61 cgcgctccgc ccgccgccga gagagccgcc agcgccgtcc gtgacctcgt gaggctggca
      121 tgcaggatgg caggacagcc cagccacatg cctcatggag ggagcccaaa ccacctctgc
      181 catgcactgg ggcctgcacc ccctccagac ccacagaggc tcccaaacac cctgtctttt
      241 cgctgctcac tggcagactt ccaaattgag aagaagattg gccgaggaca gttcagtgag
      301 gtttacaagg ccacttgcct gctggacagg aagacagtgg ctctgaagaa ggtgcagatc
      361 tttgagatga tggatgccaa agccaggcaa gactgtgtca aggaaattgg tctcctaaag
      421 caactgaacc atccgaatat catcaagtat ctggactcct tcatcgaaga caatgagctg
      481 aacattgtgc tggagttggc tgacgcgggt gacctctcac agatgatcaa gtatttcaag
      541 aagcagaagc ggcttatccc tgagaggacc gtgtggaagt acttcgtgca gctgtgcagc
      601 gctgtggagc acatgcactc ccgacgtgtg atgcaccgag acatcaagcc cgccaacgtg
      661 ttcatcacag ctacgggcat tgtgaagctt ggtgacctcg gcctgggccg cttcttcagc
      721 tcggagacca ctgcggccca ctcactagtg ggaacaccat actacatgtc acccgagaga
      781 atccatgaga atggctacaa cttcaagtca gacatctggt ccctaggctg cctgctgtat
      841 gagatggcag ctctccagag ccccttctat ggagataaga tgaatctctt ctccctctgc
      901 caaaagattg agcagtgtga ctacccgcct ctcccgggag agcactactc tgagaagctt
      961 cgggaactgg tcagtatgtg tatctaccct gaccctgacc accgacctga cattgtatat
     1021 gtgcaccagg tggctagaca gatgcacgtc tggacatcca gcacctgaac gcaacacacc
     1081 tcatcaaagc caagtcactt gtcttacttg agtcctctcc ccaagtggta cctggaagcc
     1141 cccacagcca agactagagg actctcagct ggctccaccc agcggaaaac agctgggcta
     1201 tggtcagact ccaaagtcct ttctttgaac tgtcgcagac aatctgagct gggatgagtg
     1261 gggaggggtt tggcagccat ggaaacccct tgtacctact gaaatgtgaa cctctgcctg
     1321 gttgcttcag acccgtccag gcccacacgg tcaggagact tgaagggagc tgggtgacta
     1381 gtgtgggcca gcctttgcaa atggttacca ccattggttt agttcttagt acctttccat
     1441 acaatcaggc tgtgtgcttc atttactcta ttgtacaagg atacgatgga aataatggtt
     1501 tagagtagag attgcgctaa tatttttctc tgcattttct aatttgatga gtgacagtaa
     1561 agagcaccta gcatcgggcc tttcgacata agtggcatct gggagatggg agggttctcc
     1621 tctgtctccg cagccctgcc ccgtcacatc accaccgctg ggatgctatg gctcactcta
     1681 ggatcagtgt gcagacttcg tccatccagc agggaaactc cagaagcttt gctcgcacac
     1741 tgttcttgac aagttcgggg tctggatctt cctagtgact tgtcaaagtt tgttggcctt
     1801 tgtgtccttg cctgcaaatg ggacattata ataaggtgtc tgtcttccag gtggctgaca
     1861 ggatttaaaa caaaagttgt tagccatggc agcctaagct tgctatccca gcacttgaga
     1921 ggtagaggca ggaggatcaa gaatccaagg ttgccctgag caatacagga agtttgagac
     1981 aggaacggac tacataagac cctgtctcaa aaacaaaaca aacaaaaccc aaaccaatag
     2041 agatgcaaat cagctagcag tggcaggtga tccaaaaata cagcctgcct gtcacccctt
     2101 cagaaaactc aagtcatgga ctatgcgagt caagaattaa aatacaactg tattatgtgc
     2161 aggaaaagca gaaatatgtt catttacttt ttgttcctaa taggattaac tgtgaagact
     2221 aacttataaa tgtgaattta aggaaatcta agtgctggag gaaacagtct gaattttgtt
     2281 cttctattcc agtgggtctc actttgtata tcaagacccc ctttacaaac ctgtctccaa
     2341 agatatttat attacgattc ataaccgtag caaaattaca gttatgaggt agcaaagaaa
     2401 ataattttat agctgggagt caccacaaca taaggaactg tattgaaggg tcccagcatt
     2461 gggaaggttg agaaccgctg gtctatgcag tattcaagga gcacccactt ggtgacaggc
     2521 cagtgctcct gtcttgtcag gtgatgttta ccagggctct gcacaaataa gctctctggc
     2581 tagccaccac cctcttgagc agggcctgta ctccagatac agcctctgcc cggatcaagt
     2641 gctccatatt ttagacaaat tgggaagaga gacggctgag cagctcaggg gtggccctga
     2701 ctgcgtgtcc gtgttttatc ttggactgca gtccacaccc agcacacagc agcagcataa
     2761 cttcttgagg tgcctttaga agcaacctgt caggcccaaa ccgtccaagt aacaccagaa
     2821 tattcttaag ttccccaaat aattctgaca ctcatcagcc aagctgctgc cctcaataaa
     2881 tccactgtac cctttcaagc agtttgcaca agggaatctg tcctccagtg tcctgcccag
     2941 aatactgact ctgagaaagg atcagagtcg ctgtctcctg cccagcacta actcctgaaa
     3001 gttttcctgg gtctttgtaa gaactgtttc tgttcttaga ggctctgttc tatctgccct
     3061 tggtacaagc agcctctgca aaccaaagaa cttcatttca gcactctacc cattgtccac
     3121 accaagagaa ctatctgccc aataaaatgg cccttgggat gtacagtc
//
