LOCUS       NM_175089               5419 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Mus musculus NIMA (never in mitosis gene a)-related expressed
            kinase 1 (Nek1), transcript variant 3, mRNA.
ACCESSION   NM_175089 XM_356077
VERSION     NM_175089.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Zelina,P., de Ruiter,A.A., Kolsteeg,C., van Ginneken,I., Vos,H.R.,
            Supiot,L.F., Burgering,B.M.T., Meye,F.J., Veldink,J.H., van den
            Berg,L.H. and Pasterkamp,R.J.
  TITLE     ALS-associated C21ORF2 variant disrupts DNA damage repair,
            mitochondrial metabolism, neuronal excitability and NEK1 levels in
            human motor neurons
  JOURNAL   Acta Neuropathol Commun 12 (1), 144 (2024)
   PUBMED   39227882
  REMARK    GeneRIF: ALS-associated C21ORF2 variant disrupts DNA damage repair,
            mitochondrial metabolism, neuronal excitability and NEK1 levels in
            human motor neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5419)
  AUTHORS   Wang,H., Qi,W., Zou,C., Xie,Z., Zhang,M., Naito,M.G., Mifflin,L.,
            Liu,Z., Najafov,A., Pan,H., Shan,B., Li,Y., Zhu,Z.J. and Yuan,J.
  TITLE     NEK1-mediated retromer trafficking promotes blood-brain barrier
            integrity by regulating glucose metabolism and RIPK1 activation
  JOURNAL   Nat Commun 12 (1), 4826 (2021)
   PUBMED   34376696
  REMARK    GeneRIF: NEK1-mediated retromer trafficking promotes blood-brain
            barrier integrity by regulating glucose metabolism and RIPK1
            activation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5419)
  AUTHORS   Singh,V., Jaiswal,P.K., Ghosh,I., Koul,H.K., Yu,X. and De
            Benedetti,A.
  TITLE     The TLK1-Nek1 axis promotes prostate cancer progression
  JOURNAL   Cancer Lett 453, 131-141 (2019)
   PUBMED   30928383
REFERENCE   5  (bases 1 to 5419)
  AUTHORS   Amin,P., Florez,M., Najafov,A., Pan,H., Geng,J., Ofengeim,D.,
            Dziedzic,S.A., Wang,H., Barrett,V.J., Ito,Y., LaVoie,M.J. and
            Yuan,J.
  TITLE     Regulation of a distinct activated RIPK1 intermediate bridging
            complex I and complex II in TNFalpha-mediated apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 115 (26), E5944-E5953 (2018)
   PUBMED   29891719
REFERENCE   6  (bases 1 to 5419)
  AUTHORS   Upadhya,P., Churchill,G., Birkenmeier,E.H., Barker,J.E. and
            Frankel,W.N.
  TITLE     Genetic modifiers of polycystic kidney disease in intersubspecific
            KAT2J mutants
  JOURNAL   Genomics 58 (2), 129-137 (1999)
   PUBMED   10366444
REFERENCE   7  (bases 1 to 5419)
  AUTHORS   Arama,E., Yanai,A., Kilfin,G., Bernstein,A. and Motro,B.
  TITLE     Murine NIMA-related kinases are expressed in patterns suggesting
            distinct functions in gametogenesis and a role in the nervous
            system
  JOURNAL   Oncogene 16 (14), 1813-1823 (1998)
   PUBMED   9583679
REFERENCE   8  (bases 1 to 5419)
  AUTHORS   Tanaka,K., Parvinen,M. and Nigg,E.A.
  TITLE     The in vivo expression pattern of mouse Nek2, a NIMA-related
            kinase, indicates a role in both mitosis and meiosis
  JOURNAL   Exp Cell Res 237 (2), 264-274 (1997)
   PUBMED   9434622
REFERENCE   9  (bases 1 to 5419)
  AUTHORS   Janaswami,P.M., Birkenmeier,E.H., Cook,S.A., Rowe,L.B.,
            Bronson,R.T. and Davisson,M.T.
  TITLE     Identification and genetic mapping of a new polycystic kidney
            disease on mouse chromosome 8
  JOURNAL   Genomics 40 (1), 101-107 (1997)
   PUBMED   9070925
REFERENCE   10 (bases 1 to 5419)
  AUTHORS   Letwin,K., Mizzen,L., Motro,B., Ben-David,Y., Bernstein,A. and
            Pawson,T.
  TITLE     A mammalian dual specificity protein kinase, Nek1, is related to
            the NIMA cell cycle regulator and highly expressed in meiotic germ
            cells
  JOURNAL   EMBO J 11 (10), 3521-3531 (1992)
   PUBMED   1382974
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CX237035.1, AY850065.1, AK135564.1 and BC070443.1.
            
            On Jun 11, 2014 this sequence version replaced NM_175089.3.
            
            Transcript Variant: This variant (3) lacks two alternate in-frame
            exons, compared to variant 1. The encoded protein (isoform 3) is
            shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070443.1, AY850065.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                CX237035.1         1-27
            28-4291             AY850065.1         1-4264
            4292-5274           AK135564.1         1434-2416
            5275-5419           BC070443.1         5218-5362
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 30.91 cM"
     gene            1..5419
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /note="NIMA (never in mitosis gene a)-related expressed
                     kinase 1"
                     /db_xref="GeneID:18004"
                     /db_xref="MGI:MGI:97303"
     exon            1..571
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            572..719
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     CDS             603..4214
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /EC_number="2.7.11.1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     NIMA-related kinase 1; serine/threonine-protein kinase
                     Nek1; Nek1 serine/threonine- and tyrosine-specific protein
                     kinase; nimA-related protein kinase 1; never in mitosis
                     A-related kinase 1; NY-REN-55 antigen; kidney, anemia and
                     testis"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek1 isoform 3"
                     /protein_id="NP_780298.2"
                     /db_xref="CCDS:CCDS40347.1"
                     /db_xref="GeneID:18004"
                     /db_xref="MGI:MGI:97303"
                     /translation="MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKE
                     RQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQE
                     DQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELA
                     RTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISG
                     SFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFC
                     LKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKP
                     PPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQ
                     EKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFD
                     QMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVEEFLQRKREAMQNKARAEG
                     HVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQ
                     TNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQV
                     KPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQ
                     HHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVG
                     EVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPL
                     LTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSS
                     THTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPA
                     VDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKS
                     KNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDE
                     PIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTAR
                     GTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEK
                     FFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE"
     exon            720..816
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            817..914
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            915..998
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1066
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1153
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1208
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1409
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1470
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1622
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1682
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1742
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1790
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1865
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..2029
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2132
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2216
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2294
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2390
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2519
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2579
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2808
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2961
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3126
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3247
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3336
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3337..3575
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3576..3727
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3728..3936
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..4067
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            4068..4200
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..5403
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5379..5384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /note="hexamer: ATTAAA"
     polyA_site      5403
                     /gene="Nek1"
                     /gene_synonym="D8Ertd790e; kat"
                     /note="major polyA site"
ORIGIN      
        1 gtcacacgcc agcgacagtg acgcgagggc cgttcccctc tcctcagcag tagctctatg
       61 gtttcagggc ggcaacgtgc agcgtcctta ccttgagcct gtgcagttgc cctcaccccg
      121 gaatccatag tcactgtgac gaggcgggag gacttgggcg acaggtagcc tcccagtccc
      181 acacgctgcg ggtccgcgcc tggccaagcc acctcgacct gtgaagttgg gggcggtacc
      241 cagcaactcc ccctgtgcag ccgccgtttc caaggggtca ggaaccgctg tgtttgtttc
      301 gtccgcgtag ccagggcggg tcgcggagta ctgtgcctga cccgacggtg gcaagtctga
      361 cgcgtcagcc agagaccggt gcccggtgta ggagtcgcag cctgggctgt gagcggctgc
      421 tgggtagaca gacttgcttt ctcttacagc atgtcatttc caaaatgcat cgtggtgctt
      481 ctgccttaag tcctatagga agacactgcc gccactagac cggtgcttat ggtcgccact
      541 gttattctga ctcaggtccc gtgtcattga gcatatgtat gaaaatgcct taggagggaa
      601 ccatggagaa gtatgtgaga ctgcagaaga ttggagaagg ttcatttgga aaagctgttc
      661 ttgttaaatc gacagaggat ggcagacatt atgtcatcaa ggaaattaac atctcaagaa
      721 tgtctgataa agaaaggcaa gaatcaagga gagaagttgc tgtattggca aacatgaagc
      781 atccaaatat tgtccaatat aaagaatcat ttgaagaaaa tggctctctc tacatagtaa
      841 tggattactg tgaaggaggt gatttgttta aacgaataaa tgctcagaaa ggcgctctgt
      901 ttcaagaaga ccagattttg gactggtttg tgcagatatg tttggctctg aagcatgtac
      961 atgatagaaa aattcttcac cgagacataa agtcacagaa catatttcta accaaagatg
     1021 ggacagtgca gcttggagat tttggaattg ctcgagttct taatagtact gtagagctgg
     1081 ctcgaacttg cataggcact ccatactact tgtcacctga aatctgtgaa aacaagcctt
     1141 ataacaataa aagtgacatt tgggctttgg gctgtgtcct ttatgagttg tgtacactta
     1201 aacatgcatt tgaagctgga aacatgaaaa acctggtact gaagataatc tccggatcct
     1261 ttcctccagt gtctccacat tactcctatg atctccgcag cttgctgtct cagttattta
     1321 aaagaaatcc tagggataga ccatcagtca actccatatt ggagaaaggt tttatagcta
     1381 aacgaatcga aaagtttctc tcccctcagc ttattgcaga agaattttgt ctaaaaacac
     1441 tttcaaagtt tggaccacag cctctcccag gtaaaagacc agcatcagga caaggtgtca
     1501 gttcttttgt ccctgctcag aaaatcacaa agcctgctgc taaatacgga gtgcctttaa
     1561 catataagaa gtatggagat aaaaagttac ttgagaaaaa accaccccca aaacataaac
     1621 aggcccatca aattcccgtg aagaaaatga attctggaga agaaaggaag aaaatgtctg
     1681 aggaagcagc aaaaaaaaga aggttggaat ttattgagaa agaaaagaag caaaaggatc
     1741 agattaggtt cctgaaggct gagcagatga agcggcaaga gaagcagcgg ttggagagga
     1801 taaatagggc cagggaacaa ggatggagga atgttttaag ggctggtgga agcggtgaag
     1861 taaaggcttc cttttttggc attggagggg ctgtctctcc atcaccgtgt tctcctcgag
     1921 gccagtatga acattaccat gccatttttg accaaatgca gcggctaaga gcagaagata
     1981 atgaagcaag atggaagggg ggaatctatg gtcgatggct cccagaaagg caaaaaggac
     2041 acttagctgt agagagagcc aaccaagtgg aagaattcct acagcgtaaa cgagaagcta
     2101 tgcagaataa agcccgagcc gaaggacacg tggtttattt ggcaagactg aggcaaataa
     2161 gactacaaaa ttttaatgag cgccaacaga ttaaagccaa acttcgtggt gagaataaag
     2221 aagctgatgg taccaaagga caagaagcaa ctgaagagac tgacatgagg ctcaaaaaga
     2281 tggagtcact taaggcgcaa acaaatgcac gtgctgctgt actaaaagaa cagctggagc
     2341 gaaaaagaaa ggaagcttat gaaagagaaa agaaagtatg ggaagaacat ttggtggcga
     2401 gggtaaaaag ctcagatgtt cctctgcctt tggaacttct tgaaacaggt ggttctccat
     2461 caaagcagca ggtgaagcct gtcatttctg tgacttcagc tttgaaagaa gtgggcctgg
     2521 atggaagttt aactgatacc caggaagaag aaatggaaaa gagtaacagt gctatttcaa
     2581 gtaagcgaga aatcctgcgt aggctaaatg aaaatcttaa agctcaagag gatgaaaagg
     2641 aaaagcagca tcactcaggt tcttgtgaga ccgttggtca caaagatgag agagagtatg
     2701 agacagaaaa tgccatttcc tctgatcgca agaagtggga gatgggaggt cagcttgtga
     2761 ttcctctcga tgcagtgaca ctggatacat ccttctctgc aaccgaaaaa catactgtgg
     2821 gagaggttat taaattagat tctaatggct ctccaagaaa agtctggggg aaaaacccta
     2881 cagattctgt gctgaagata cttggagaag ctgaattaca gctacagaca gaactactag
     2941 aaaacacatc ttttaaaagt gaggtttatg ctgaagagga gaactacaaa cccttactta
     3001 ctgaagaaga gaatctgcag tgcatttcaa aagaaataaa tccatcagct actgttgatt
     3061 ctactgaaac gaaaagtcca aagtttactg aggtgtctcc acaaatgtca gaaggaaatg
     3121 tggaagaacc tgatgatttg gaaacagaag ttctacaaga gccaagtagc acacacacag
     3181 atgggagttt gccacctgtt cttaatgatg tgtggactag agagaaggaa gcagctaagg
     3241 aaactgagtt ggaagataag gttgctgtgc agcagagtga agtttgtgaa gatagaattc
     3301 cagggaacgt ggaccaatcc tgtaaggatc agagagatcc tgcagtagac gattctccgc
     3361 agtctggctg tgatgtagag aagtcagtac agccagaatc gattttccag aaagtggttc
     3421 attctaagga cttgaactta gttcaggcag ttcattgctc accagaagaa ccaattccaa
     3481 ttcgatctca ctctgattct ccaccaaaaa ctaagagcaa gaattcctta ctgattggac
     3541 tttcaactgg tctgtttgat gcaaacaatc caaagatgct gaggacctgc tcacttccag
     3601 atctttccaa gctgttcaga accctaatgg acgttcccac tgtgggggac gttcatcaag
     3661 acagtcttga aatcgatgag ctggaagatg aaccaattaa agaagggcct tctgattccg
     3721 aagacactgt atttgaagaa actgacacag atttacaaga gcttcaggcc tcaatggagc
     3781 agctgcttag ggagcaacca ggtgacgaat acagtgagga ggaagagtct gttttaaaaa
     3841 gcagcgatgt ggagcagaca gcaagaggga cagatgcccc agacgaggag gacaacccca
     3901 gcagcgaaag cgccctgaac gaggaatggc actcagataa tagtgacgct gagaccacta
     3961 gtgaatgtga atatgacagt gtctttaacc atttagagga actaagactt cacttggagc
     4021 aagaaatggg ctttgaaaag ttctttgagg tttatgagaa agtaaaggct attcatgagg
     4081 atgaagatga aaatattgaa atttgttcaa caatagttga gaatattttg ggcaatgagc
     4141 accagcatct ctatgccaag attctgcatt tagtcatggc agatggagcc tatcaggaag
     4201 ataatgatga ataatcctca ggacattctt taatagtcaa ctgtaagaac acatttgaac
     4261 ttggctcata atacaagctt cctgggaaat agagattatt tatgagaaat accagattta
     4321 agatgggcat gcttattgca tgaaaaaaat taagaagtgt gatatttttc ctcacgtttt
     4381 atctacctta ttttgtttat tggattcttc agctaactct catagaatgt atgtttcttt
     4441 aaatcacctc accaaagcat tgagtcaact gaaaatttat tgtgcaataa ggaactactt
     4501 ttagcaactg tctttctgtg gaagagttgg ctatatcaaa aaaggcctgc ttttggtgtt
     4561 tcatttaaat ttcagcatga cttttttttt ttctgtctaa cattttttat cctggctagc
     4621 agttcacatt aaataattct tgtcaagggc tctattcaag tatctacatt tagaagatga
     4681 aattctagcc ttgaagtttg tattctcgag ttattttcaa ccactggatc tctccctgag
     4741 ctagcccgat gaaaacaggc tatagaagca atcctaaaaa tcattgaaag atttaatttt
     4801 gaaggaatag caaagttata tttcctgaca cttgaaagtt ccctgattct tctgtttctc
     4861 tttatagacc agaaacgctg tatctaaaaa tcaggctata aatttaaacc tagcatatag
     4921 cataaaattg ccattttggt ttttagctcc tcgcatacat gtgcctccgt ggtcacctgt
     4981 gttaaaaagg agagagggcc ctttttcttt tggcactgtg acaatttagt aactactgtg
     5041 gggaagtgtt tgccttttat tttcagtgtg aggatttaac caggttatta caatgatggt
     5101 cacatagttt cagttatgtg aacctatata tagaaacagt tatagtttca gaatttgcca
     5161 agtatacatt gtgtcattct ctacagtcca ggtgttccta ataaaataaa agcagaaatg
     5221 tgtgcttttt ggtaccaatc agcctgattg gtgtaagctg tcatctgaaa catggaatct
     5281 ttccttgtag cactattagt actgtgttga ctttattatg tatattattt ctaacatcca
     5341 aaagtaaata gctgattttt atatttttat tttgtactat taaattttta ttatgtacct
     5401 gaaaaaaaaa aaaaaaaaa
//
