LOCUS       NM_010910               3395 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus neurofilament, light polypeptide (Nefl), mRNA.
ACCESSION   NM_010910 XM_127760
VERSION     NM_010910.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   McRae,H.M., Leong,M.P.Y., Bergamasco,M.I., Garnham,A.L., Hu,Y.,
            Corbett,M.A., Whitehead,L., El-Saafin,F., Sheikh,B.N., Wilcox,S.,
            Hannan,A.J., Gecz,J., Smyth,G.K., Thomas,T. and Voss,A.K.
  TITLE     Loss of PHF6 causes spontaneous seizures, enlarged brain ventricles
            and altered transcription in the cortex of a mouse model of the
            Borjeson-Forssman-Lehmann intellectual disability syndrome
  JOURNAL   PLoS Genet 20 (10), e1011428 (2024)
   PUBMED   39405291
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Zaninello,M., Schlegel,T., Nolte,H., Pirzada,M., Savino,E.,
            Barth,E., Klein,I., Wustenberg,H., Uddin,T., Wolff,L., Wirth,B.,
            Lehmann,H.C., Cioni,J.M., Langer,T. and Rugarli,E.I.
  TITLE     CLUH maintains functional mitochondria and translation in
            motoneuronal axons and prevents peripheral neuropathy
  JOURNAL   Sci Adv 10 (22), eadn2050 (2024)
   PUBMED   38809982
REFERENCE   3  (bases 1 to 3395)
  AUTHORS   Eguchi,T., Tezuka,T., Watanabe,Y., Inoue-Yamauchi,A., Sagara,H.,
            Ozawa,M. and Yamanashi,Y.
  TITLE     Calcium-binding protein 7 expressed in muscle negatively regulates
            age-related degeneration of neuromuscular junctions in mice
  JOURNAL   iScience 27 (2), 108997 (2024)
   PUBMED   38327785
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3395)
  AUTHORS   Zhou,J., Shi,Q., Ge,Y.Y., He,W., Hu,X., Xia,W. and Yan,R.
  TITLE     Reticulons 1 and 3 are essential for axonal growth and synaptic
            maintenance associated with intellectual development
  JOURNAL   Hum Mol Genet 32 (16), 2587-2599 (2023)
   PUBMED   37228035
REFERENCE   5  (bases 1 to 3395)
  AUTHORS   Qu,F., Li,W., Xu,J., Zhang,R., Ke,J., Ren,X., Meng,X., Qin,L.,
            Zhang,J., Lu,F., Zhou,X., Luo,X., Zhang,Z., Wang,M., Wu,G., Pei,D.,
            Chen,J., Cui,G., Suo,S. and Peng,G.
  TITLE     Three-dimensional molecular architecture of mouse organogenesis
  JOURNAL   Nat Commun 14 (1), 4599 (2023)
   PUBMED   37524711
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3395)
  AUTHORS   Puschel,A.W., Westerfield,M. and Dressler,G.R.
  TITLE     Comparative analysis of Pax-2 protein distributions during
            neurulation in mice and zebrafish
  JOURNAL   Mech Dev 38 (3), 197-208 (1992)
   PUBMED   1457381
REFERENCE   7  (bases 1 to 3395)
  AUTHORS   Beaudet,L., Charron,G., Houle,D., Tretjakoff,I., Peterson,A. and
            Julien,J.P.
  TITLE     Intragenic regulatory elements contribute to transcriptional
            control of the neurofilament light gene
  JOURNAL   Gene 116 (2), 205-214 (1992)
   PUBMED   1634118
REFERENCE   8  (bases 1 to 3395)
  AUTHORS   Ivanov,T.R. and Brown,I.R.
  TITLE     Interaction of multiple nuclear proteins with the promoter region
            of the mouse 68-kDa neurofilament gene
  JOURNAL   J Neurosci Res 32 (2), 149-158 (1992)
   PUBMED   1404490
REFERENCE   9  (bases 1 to 3395)
  AUTHORS   Shneidman,P.S., Bruce,J., Schwartz,M.L. and Schlaepfer,W.W.
  TITLE     Negative regulatory regions are present upstream in the three mouse
            neurofilament genes
  JOURNAL   Brain Res Mol Brain Res 13 (1-2), 127-138 (1992)
   PUBMED   1315909
REFERENCE   10 (bases 1 to 3395)
  AUTHORS   Sihag,R.K. and Nixon,R.A.
  TITLE     Identification of Ser-55 as a major protein kinase A
            phosphorylation site on the 70-kDa subunit of neurofilaments. Early
            turnover during axonal transport
  JOURNAL   J Biol Chem 266 (28), 18861-18867 (1991)
   PUBMED   1717455
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK149219.1, BC029203.1, AC154687.2 and BE952212.1.
            
            On Apr 17, 2018 this sequence version replaced NM_010910.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149219.1, SRR1660819.54151.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AK149219.1         4-24
            22-2030             BC029203.1         1-2009
            2031-2623           AK149219.1         2041-2633
            2624-2806           AC154687.2         94704-94886
            2807-3395           BE952212.1         1-589               c
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 35.46 cM"
     gene            1..3395
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="neurofilament, light polypeptide"
                     /db_xref="GeneID:18039"
                     /db_xref="MGI:MGI:97313"
     exon            1..1147
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1732
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="68 kDa neurofilament protein; neurofilament triplet
                     L protein"
                     /codon_start=1
                     /product="neurofilament light polypeptide"
                     /protein_id="NP_035040.1"
                     /db_xref="CCDS:CCDS27232.1"
                     /db_xref="GeneID:18039"
                     /db_xref="MGI:MGI:97313"
                     /translation="MSSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYS
                     APVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFA
                     SFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQAL
                     QGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMD
                     EIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQN
                     AEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQL
                     QELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRK
                     LLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQE
                     EQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKE
                     EEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD"
     exon            1148..1272
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1592
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..3380
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2154..2159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="hexamer: AATAAA"
     polyA_site      2175
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="major polyA site"
     regulatory      2812..2817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="hexamer: AATAAA"
     polyA_site      2836
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
     regulatory      2968..2973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
                     /note="hexamer: AATATA"
     polyA_site      2990
                     /gene="Nefl"
                     /gene_synonym="CMT2E; NF-L; NF68; Nfl"
ORIGIN      
        1 gcacacagcc atccatcctc ccctttccct cttctccccc gttcttctct ctaggtcccc
       61 catctccgcc ccgggcctga ggggcgcacc ggccgccacc atgagttcgt tcggctacga
      121 tccgtacttt tcgacctcct acaagcggcg ctatgtggag acgccccggg tgcacatctc
      181 cagcgtgcgc agcggctaca gcacggcgcg ctccgcgtac tccagctact ccgcgccggt
      241 ctcctcctcg ctgtccgtgc gccgcagcta ctcgtccagc tctggctctt tgatgcccag
      301 cctggagaat ctcgatctga gccaggtagc cgccatcagc aacgacctca agtctatccg
      361 cacacaagag aaggcacagc tgcaggacct caacgatcgc ttcgccagct tcatcgagcg
      421 cgtgcacgag ctggagcagc agaacaaggt cctggaagcc gagctgttgg tgctgcgcca
      481 gaaacactct gagccttccc gcttccgcgc cctgtacgag caggagatcc gcgatctgcg
      541 gctggcagcg gaagacgcca ctaacgagaa gcaggcgctg cagggcgagc gcgaggggct
      601 ggaggagact ctgcgcaacc tgcaggctcg ctatgaggaa gaagtgctga gccgcgagga
      661 cgccgagggc cggctgatgg aagcgcgcaa aggtgcggat gaggccgcgc tcgcccgcgc
      721 cgagctggag aagcgcatcg acagcctgat ggacgagata gctttcctga agaaggtgca
      781 cgaggaagag atcgccgagc tgcaggctca gatccagtat gctcagatct ccgtggagat
      841 ggacgtgtcc tccaagcccg acctctccgc cgctctcaag gacatccgcg ctcagtacga
      901 gaagctggcc gccaagaaca tgcagaacgc cgaagagtgg ttcaagagcc gcttcaccgt
      961 gctaaccgag agcgccgcca agaacaccga cgctgtgcgc gctgccaagg acgaggtgtc
     1021 ggaaagccgc cgcctgctca aggctaagac cctggagatc gaagcctgcc ggggtatgaa
     1081 cgaagctctg gagaagcagc tgcaggagct agaggacaag cagaatgcag acattagcgc
     1141 catgcaggac acaatcaaca aactggagaa tgagctgaga agcacgaaga gcgagatggc
     1201 caggtacctg aaggagtacc aggacctcct caatgtcaag atggccttgg acatcgagat
     1261 tgcagcttac agaaaactct tggaaggcga agagaccagg ctcagtttca ccagcgtggg
     1321 tagcataacc agcggctact ctcagagctc gcaggtcttc ggccgttctg cttacagtgg
     1381 cttgcagagc agctcctact tgatgtctgc tcgctctttc ccagcctact ataccagcca
     1441 cgtccaggaa gagcagacag aggtcgagga gaccattgag gctacgaaag ctgaggaggc
     1501 caaggatgag cccccctctg aaggagaagc agaagaggag gagaaggaga aagaggaggg
     1561 agaggaagag gaaggcgctg aggaggaaga agctgccaag gatgagtctg aagacacaaa
     1621 agaagaagaa gaaggtggtg agggtgagga ggaagacacc aaagaatctg aagaggaaga
     1681 gaagaaagag gagagtgctg gagaggagca ggtggctaag aagaaagatt gagccctatt
     1741 cccaactatt ccaggaaaag ttctccccaa tcaggtcaac ctcatcacca accaaccagt
     1801 tgagttccag atcctataca aattaagaag tcaatacatg tataattctg agatgactta
     1861 ggttggactt tcaatgttgt gctatgaatt tcctccttac gcagagtatc tgtttgcttg
     1921 cagagtggct ttctggcttg ctgccagcct gtgcatggtc catgcttatg agttcaggat
     1981 ctatggcaat gtgaatcaca cagatgttta caataataat aaaaaaaaaa ccacacacac
     2041 aacacgaata aatgaattca ctaatgttca tgtgcaacct catgggaaaa atagtaactt
     2101 gaatcttcag tggttagaaa ttaaagacct cgagttatgt gcaataaata gtaaataaag
     2161 ttacactgaa tgacatattt tgctgtggtt tttacttaat taaagtacct taaataaggc
     2221 accaacataa accataggta agtggacgac tgacctccag ttttttataa agtaaaagtt
     2281 gtagtgagtg cagtagataa ttggacattc tgagggatag acaaataggt caagtctaga
     2341 aaatatttca aacgttactg ataataaata tgaatgagac cattcataaa taagagcgtc
     2401 attcagcatg caaaacacac aatgctagct ggagggatgg cttcctaaaa gacggttttg
     2461 aaattatgat ggactattat atagtagtgc aacctggatg gactatgaat cccacacagt
     2521 tgtcacggtg gttacatttt taatatatcc ttgaatatcg ggcataaatt gaaacctgag
     2581 cccatgatac aattaagagg aatgtcatgg gggggcggag aggagtaaga ttatcagagg
     2641 ttatctgcca ctgcaaacat gttgcctgga atcctgagct catgaatggt gtgttgtctg
     2701 cttaattcta ctgcctcaca taattgcctt tgatgctata catttaaaaa ccctttaaag
     2761 accacacaag cagataatgg gattacatta ctgtcacttc ttgggggaga gaataaattt
     2821 gcgtgctgca ttggtattgc tctgcatgtc ttaattggta actatttgga tttcaatgcc
     2881 tggttggaca ctacatcatc tttcccccat ccatccacat ccatggtaaa gccacgacaa
     2941 agcttgcact acttgtgttt ggtgtggaat atagatgacg tctttgccta tctacagctc
     3001 tgttgtactt ctgcggctct gatgttccta ggaactgctt ggtttgttga tttcttcact
     3061 acctgttttt tagacttaga aattttatct aaaactgaag tgatctgcaa ttgaagtctc
     3121 tttgtttcac ttacctctac ttgaatatgt tactacacaa ataggcaaag catttcttta
     3181 tcatgatgtg agtttgaact atgaaggctc ttggcattcc tttgtagata ggaagttcct
     3241 tgtctgcaag cttttcacac atgtcagtca tgagtgaatg caaacatctg gttggtttat
     3301 aaaatacttg attgaagaac acagttccat attcaatcct gtgtgccata ataaaatatt
     3361 gaaaaaaaat ctaaattcaa aaaaaaaaaa aaaaa
//
