LOCUS       NP_059492                833 aa            linear   ROD 03-JUN-2025
DEFINITION  enhancer of filamentation 1 isoform 2 [Mus musculus].
ACCESSION   NP_059492
VERSION     NP_059492.3
DBSOURCE    REFSEQ: accession NM_017464.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 833)
  AUTHORS   Short,K.L., Lao,J., Lam,R., Moreau,J.L.M., Ng,J., Piran,M.,
            Combes,A.N., Cottle,D.L. and Cole,T.J.
  TITLE     Disrupted glucocorticoid receptor cell signalling causes a
            ciliogenesis defect in the fetal mouse renal tubule
  JOURNAL   EMBO Rep (2025) In press
   PUBMED   40247090
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (residues 1 to 833)
  AUTHORS   Wong,W., Estep,J.A., Treptow,A.M., Rajabli,N., Jahncke,J.N.,
            Ubina,T., Wright,K.M. and Riccomagno,M.M.
  TITLE     An adhesion signaling axis involving Dystroglycan, beta1-Integrin,
            and Cas adaptor proteins regulates the establishment of the
            cortical glial scaffold
  JOURNAL   PLoS Biol 21 (8), e3002212 (2023)
   PUBMED   37540708
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 833)
  AUTHORS   Rusyn,L., Reinartz,S., Nikiforov,A., Mikhael,N., Vom Stein,A.,
            Kohlhas,V., Bloehdorn,J., Stilgenbauer,S., Lohneis,P., Buettner,R.,
            Robrecht,S., Fischer,K., Pallasch,C., Hallek,M., Nguyen,P.H. and
            Seeger-Nukpezah,T.
  TITLE     The scaffold protein NEDD9 is necessary for leukemia-cell migration
            and disease progression in a mouse model of chronic lymphocytic
            leukemia
  JOURNAL   Leukemia 36 (7), 1794-1805 (2022)
   PUBMED   35523865
  REMARK    GeneRIF: The scaffold protein NEDD9 is necessary for leukemia-cell
            migration and disease progression in a mouse model of chronic
            lymphocytic leukemia.
REFERENCE   4  (residues 1 to 833)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 833)
  AUTHORS   Deneka,A.Y., Kopp,M.C., Nikonova,A.S., Gaponova,A.V.,
            Kiseleva,A.A., Hensley,H.H., Flieder,D.B., Serebriiskii,I.G. and
            Golemis,E.A.
  TITLE     Nedd9 Restrains Autophagy to Limit Growth of Early Stage Non-Small
            Cell Lung Cancer
  JOURNAL   Cancer Res 81 (13), 3717-3726 (2021)
   PUBMED   34006524
  REMARK    GeneRIF: Nedd9 Restrains Autophagy to Limit Growth of Early Stage
            Non-Small Cell Lung Cancer.
REFERENCE   6  (residues 1 to 833)
  AUTHORS   Seo,S., Asai,T., Saito,T., Suzuki,T., Morishita,Y., Nakamoto,T.,
            Ichikawa,M., Yamamoto,G., Kawazu,M., Yamagata,T., Sakai,R.,
            Mitani,K., Ogawa,S., Kurokawa,M., Chiba,S. and Hirai,H.
  TITLE     Crk-associated substrate lymphocyte type is required for lymphocyte
            trafficking and marginal zone B cell maintenance
  JOURNAL   J Immunol 175 (6), 3492-3501 (2005)
   PUBMED   16148091
  REMARK    GeneRIF: Cas-L affects homeostasis of MZB cells and peripheral
            lymphoid organs, which is considered to be relevant to impaired
            lymphocyte migration and adhesion
REFERENCE   7  (residues 1 to 833)
  AUTHORS   Abramova,N., Charniga,C., Goderie,S.K. and Temple,S.
  TITLE     Stage-specific changes in gene expression in acutely isolated mouse
            CNS progenitor cells
  JOURNAL   Dev Biol 283 (2), 269-281 (2005)
   PUBMED   15890332
REFERENCE   8  (residues 1 to 833)
  AUTHORS   Cai,D., Felekkis,K.N., Near,R.I., O'Neill,G.M., van Seventer,J.M.,
            Golemis,E.A. and Lerner,A.
  TITLE     The GDP exchange factor AND-34 is expressed in B cells, associates
            with HEF1, and activates Cdc42
  JOURNAL   J Immunol 170 (2), 969-978 (2003)
   PUBMED   12517963
REFERENCE   9  (residues 1 to 833)
  AUTHORS   Sakakibara,A. and Hattori,S.
  TITLE     Chat, a Cas/HEF1-associated adaptor protein that integrates
            multiple signaling pathways
  JOURNAL   J Biol Chem 275 (9), 6404-6410 (2000)
   PUBMED   10692442
REFERENCE   10 (residues 1 to 833)
  AUTHORS   Kumar,S., Tomooka,Y. and Noda,M.
  TITLE     Identification of a set of genes with developmentally
            down-regulated expression in the mouse brain
  JOURNAL   Biochem Biophys Res Commun 185 (3), 1155-1161 (1992)
   PUBMED   1378265
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK033729.1, AK054179.1, AA762918.1, AA198880.1 and AV367231.2.
            
            On Jun 10, 2007 this sequence version replaced NP_059492.2.
            
            Transcript Variant: This variant (2) contains an alternate 5'-most
            exon and initiates translation at an alternate start codon,
            compared to variant 1. The encoded isoform (2) has a distinct
            N-terminus and is the same length as isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046357.1, AK054179.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 20.43 cM"
     Protein         1..833
                     /product="enhancer of filamentation 1 isoform 2"
                     /note="enhancer of filamentation 1; Crk-associated
                     substrate lymphocyte-specific protein; CRK-associated
                     substrate-related protein"
                     /calculated_mol_wt=92927
     Region          7..63
                     /region_name="SH3_NEDD9"
                     /note="Src homology 3 domain of CAS (Crk-Associated
                     Substrate) scaffolding protein family member, Neural
                     precursor cell Expressed, Developmentally Down-regulated
                     9; cd12002"
                     /db_xref="CDD:212935"
     Site            order(12,14,17,21,42..43,56,58..59)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212935"
     Site            91
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            163
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            165
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            176
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            188
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            213
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            222
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Region          237..258
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Site            295
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Region          297..316
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Site            316
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Region          326..403
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Region          350..833
                     /region_name="Interacts with CTTN.
                     /evidence=ECO:0000250|UniProtKB:Q14511"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Region          359..362
                     /region_name="Caspase cleavage related site.
                     /evidence=ECO:0000250|UniProtKB:Q14511"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Site            368
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Region          400..560
                     /region_name="Serine_rich_NEDD9"
                     /note="Serine rich Four helix bundle domain of CAS
                     (Crk-Associated Substrate) scaffolding protein, Neural
                     precursor cell Expressed, Developmentally Down-regulated
                     9; a protein interaction module; cd11550"
                     /db_xref="CDD:211404"
     Region          560..623
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Region          618..827
                     /region_name="CAS_C"
                     /note="Crk-Associated Substrate C-terminal domain;
                     pfam12026"
                     /db_xref="CDD:463437"
     Region          709..833
                     /region_name="Required for interaction with PLK1.
                     /evidence=ECO:0000250|UniProtKB:Q14511"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Region          709..759
                     /region_name="Divergent helix-loop-helix motif"
                     /note="propagated from UniProtKB/Swiss-Prot (O35177.2)"
     Site            779
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     Site            803
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q14511; propagated from
                     UniProtKB/Swiss-Prot (O35177.2)"
     CDS             1..833
                     /gene="Nedd9"
                     /gene_synonym="Cas-L; CasL; HEF1; MEF1; Nedd-9; p105"
                     /coded_by="NM_017464.5:138..2639"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS36640.2"
                     /db_xref="GeneID:18003"
                     /db_xref="MGI:MGI:97302"
ORIGIN      
        1 mkyknlmara lydnvpecae elafrkgdil tvieqntggl egwwlcslhg rqgivpgnrv
       61 klligpvqet pgheqptpgp mhqtfgqqkl yqvpnsqaas rdtiyqvpps yqnqgiyqvp
      121 tghgtpeqdv yqvppsvqrn iggtngplls kkvitpvrtg hgyvyeypsr yqkdvydvpp
      181 shstqgvydi ppssvkgpvf svpvgeikpq gvydipptqg vyaippsacr deaglrekey
      241 dfpppmkqdg kpdtrpegvy dipptstkta gkdlhikfpc dapggvepma rrhqsfslhh
      301 apsqlgqsgd tqsdaydvpr gvqflevpte tsekanpeer dgvydvplhn padakgsrdv
      361 vdginrlsfs stgstrsnms tsstsskess lsaspsqdkr lrldpdtaie klyrlqqtle
      421 mgvcslmslv ttdwrcygym erhineirta vdkvelflre ylhfakgala nasclpelvl
      481 hnkmkrelqr vedshqilsq tshdlnecsw slnilainkp qnkcddldrf vmvaktvpdd
      541 akqltttist yaetlfradp anshlkngpn simnsseyth pgsqmqplhp gdykaqvhsk
      601 plppslskdq ppdcgssdgs erswmddydy vhlqgkeefe rqqkelleke nimkqskaql
      661 ehhqlsqfql leqeitkpve ndiskwkpsq slpttnnsvg aqdrqllcfy ydqcethfis
      721 llnaidalfs cvssaqppri fvahskfvil sahklvfigd tltrqvaaqd irnkvrnssn
      781 qlceqlktiv matkmaalhy psttalqemv hqvtdlsrna qlfkrsllem atf
//
