LOCUS       NM_001114386            8202 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus neural precursor cell expressed, developmentally
            down-regulated gene 4-like (Nedd4l), transcript variant 1, mRNA.
ACCESSION   NM_001114386
VERSION     NM_001114386.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Liu,H., Wang,N., Yang,R., Luan,J., Cao,M., Zhai,C., Wang,S.,
            Wei,M., Wang,D., Qiao,J., Liu,Y., She,W., Guo,N., Liao,B. and
            Gou,X.
  TITLE     E3 Ubiquitin Ligase NEDD4L Negatively Regulates Skin Tumorigenesis
            by Inhibiting IL-6/GP130 Signaling Pathway
  JOURNAL   J Invest Dermatol 144 (11), 2453-2464 (2024)
   PUBMED   38580105
  REMARK    GeneRIF: E3 Ubiquitin Ligase NEDD4L Negatively Regulates Skin
            Tumorigenesis by Inhibiting IL-6/GP130 Signaling Pathway.
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Blackburn,D.M., Sahinyan,K., Hernandez-Corchado,A., Lazure,F.,
            Richard,V., Raco,L., Perron,G., Zahedi,R.P., Borchers,C.H.,
            Lepper,C., Kawabe,H., Jahani-Asl,A., Najafabadi,H.S. and
            Soleimani,V.D.
  TITLE     The E3 ubiquitin ligase Nedd4L preserves skeletal muscle stem cell
            quiescence by inhibiting their activation
  JOURNAL   iScience 27 (7), 110241 (2024)
   PUBMED   39015146
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8202)
  AUTHORS   Han,F., Wu,S., Dong,Y., Liu,Y., Sun,B. and Chen,L.
  TITLE     Aberrant expression of NEDD4L disrupts mitochondrial homeostasis by
            downregulating CaMKKbeta in diabetic kidney disease
  JOURNAL   J Transl Med 22 (1), 465 (2024)
   PUBMED   38755664
  REMARK    GeneRIF: Aberrant expression of NEDD4L disrupts mitochondrial
            homeostasis by downregulating CaMKKbeta in diabetic kidney disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8202)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 8202)
  AUTHORS   Su,W., Yu,X., Wang,S., Wang,X., Dai,Z. and Li,Y.
  TITLE     METTL3 regulates TFRC ubiquitination and ferroptosis through
            stabilizing NEDD4L mRNA to impact stroke
  JOURNAL   Cell Biol Toxicol 40 (1), 8 (2024)
   PUBMED   38302612
  REMARK    GeneRIF: METTL3 regulates TFRC ubiquitination and ferroptosis
            through stabilizing NEDD4L mRNA to impact stroke.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8202)
  AUTHORS   Flasza,M., Gorman,P., Roylance,R., Canfield,A.E. and Baron,M.
  TITLE     Alternative splicing determines the domain structure of WWP1, a
            Nedd4 family protein
  JOURNAL   Biochem Biophys Res Commun 290 (1), 431-437 (2002)
   PUBMED   11779188
REFERENCE   7  (bases 1 to 8202)
  AUTHORS   Debonneville,C., Flores,S.Y., Kamynina,E., Plant,P.J., Tauxe,C.,
            Thomas,M.A., Munster,C., Chraibi,A., Pratt,J.H., Horisberger,J.D.,
            Pearce,D., Loffing,J. and Staub,O.
  TITLE     Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+)
            channel cell surface expression
  JOURNAL   EMBO J 20 (24), 7052-7059 (2001)
   PUBMED   11742982
REFERENCE   8  (bases 1 to 8202)
  AUTHORS   Harvey,K.F., Dinudom,A., Cook,D.I. and Kumar,S.
  TITLE     The Nedd4-like protein KIAA0439 is a potential regulator of the
            epithelial sodium channel
  JOURNAL   J Biol Chem 276 (11), 8597-8601 (2001)
   PUBMED   11244092
REFERENCE   9  (bases 1 to 8202)
  AUTHORS   Kamynina,E., Debonneville,C., Bens,M., Vandewalle,A. and Staub,O.
  TITLE     A novel mouse Nedd4 protein suppresses the activity of the
            epithelial Na+ channel
  JOURNAL   FASEB J 15 (1), 204-214 (2001)
   PUBMED   11149908
REFERENCE   10 (bases 1 to 8202)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131709.3, AC102333.10, AC161175.5 and AC102288.11.
            
            On Apr 7, 2022 this sequence version replaced NM_001114386.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-388               AC131709.3         35071-35458         c
            389-462             AC102333.10        109519-109592
            463-544             AC102333.10        186895-186976
            545-583             AC161175.5         154539-154577
            584-637             AC161175.5         156859-156912
            638-688             AC161175.5         220637-220687
            689-750             AC102288.11        65798-65859
            751-853             AC102288.11        66188-66290
            854-1020            AC102288.11        68387-68553
            1021-1156           AC102288.11        72057-72192
            1157-1333           AC102288.11        73537-73713
            1334-1408           AC102288.11        76114-76188
            1409-1468           AC102288.11        78056-78115
            1469-1600           AC102288.11        82817-82948
            1601-1720           AC102288.11        83458-83577
            1721-1918           AC102288.11        84587-84784
            1919-1996           AC102288.11        89461-89538
            1997-2051           AC102288.11        91870-91924
            2052-2110           AC102288.11        96868-96926
            2111-2176           AC102288.11        101979-102044
            2177-2406           AC102288.11        103618-103847
            2407-2528           AC102288.11        105602-105723
            2529-2599           AC102288.11        106457-106527
            2600-2695           AC102288.11        109173-109268
            2696-2769           AC102288.11        114418-114491
            2770-2830           AC102288.11        116175-116235
            2831-2890           AC102288.11        118591-118650
            2891-2998           AC102288.11        118934-119041
            2999-3095           AC102288.11        120195-120291
            3096-3168           AC102288.11        120805-120877
            3169-8202           AC102288.11        123326-128359
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.57 cM"
     gene            1..8202
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /note="neural precursor cell expressed, developmentally
                     down-regulated gene 4-like"
                     /db_xref="GeneID:83814"
                     /db_xref="MGI:MGI:1933754"
     exon            1..388
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     CDS             341..3271
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /EC_number="2.3.2.26"
                     /EC_number="2.3.2.36"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase NEDD4-like; neural precursor cell
                     expressed, developmentally down-regulated gene 4b;
                     HECT-type E3 ubiquitin transferase NED4L"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase NEDD4-like isoform
                     1"
                     /protein_id="NP_001107858.1"
                     /db_xref="CCDS:CCDS50309.1"
                     /db_xref="GeneID:83814"
                     /db_xref="MGI:MGI:1933754"
                     /translation="MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPY
                     VKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRD
                     DFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQD
                     EENSEQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRS
                     TQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWET
                     ISEEMNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQITPDSNGEQFSSLIQRE
                     PSSRLRSCSVTDTVAEQAHLPPPSTPTRRARSSTVTGGEESTPSVAYVHTTPGLPSGW
                     EERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLVEPQIRRPRSLSS
                     PTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRI
                     APNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTF
                     YIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLH
                     RNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYG
                     LFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMM
                     LGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEI
                     MVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGL
                     GDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGF
                     AELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGF
                     EGVD"
     exon            389..462
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            463..544
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            545..583
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            584..637
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            638..688
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            689..750
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            751..853
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1020
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1156
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1333
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1408
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1468
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1600
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1720
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1918
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..1996
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2051
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2110
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2176
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2406
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2528
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2599
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2695
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2696..2769
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2830
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2831..2890
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..2998
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3095
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..3168
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..8202
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3675..3680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /note="hexamer: ATTAAA"
     polyA_site      3702
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /note="major polyA site"
     regulatory      8179..8184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
                     /note="hexamer: AATAAA"
     polyA_site      8202
                     /gene="Nedd4l"
                     /gene_synonym="1300012C07Rik; Nedd4-2; NEDD4.2; Nedd4b"
ORIGIN      
        1 gagagccggc cgggcggagc aggcgagggg cgggcgcgcg ggtcccgggg atcgccgagc
       61 gggcttgggg aggccgggag agccatcggc gggggcggga gcccggcggg ctcagcgcag
      121 agcagggaga gctccgggcc agtcgccgtt cggcgcgctc tcgggagccg ccagtccgcg
      181 cgtccccgca gccttccggg aggaagcggc gccgcggccg ggtgcagcct cacctgccgg
      241 cccttccccg ccgggcggcg cagaaggaca gagggtcgcg gctccgcgac gcacttcagc
      301 cagtcgcagc gtccaggacc ctgcgcgggg cgccggctcc atggcgaccg ggcttgggga
      361 gcctgtctac ggactttcgg aagaggaggg agaatcccgt atcctcagag taaaagttgt
      421 ttctggaatt gacctcgcca agaaggacat atttggagcc agcgacccat acgtgaagct
      481 gtccttgtat gtagctgatg agaatagaga acttgctctg gttcaaacca agacaattaa
      541 aaagacgctg aacccaaagt ggaatgaaga gttttatttt agggtaaatc catctaatca
      601 caggctccta tttgaagtat ttgacgagaa cagattgaca cgagacgact tcctgggcca
      661 ggtggacgtg cctcttagtc accttccgac agaagatcca accatggaga gaccctatac
      721 atttaaggat tttctcctgc gacctagaag tcataaatct cgagtcaagg ggtttttgag
      781 gttgaaaatg gcctacatgc cgaaaaacgg aggtcaggat gaagaaaaca gcgagcagag
      841 ggatgacatg gagcatggat gggaagtggt tgactccaac gactcagctt cccagcacca
      901 ggaggagctc ccccctcctc ccctgccgcc aggatgggaa gagaaagtgg acaatttagg
      961 ccgaacttac tatgtcaacc acaacaacag gagtactcag tggcaccgac ccagcttgat
     1021 ggatgtgtcc tcggaatcag acaataacat caggcagatc aaccaggagg ctgcacaccg
     1081 acgcttccgc tctcggaggc acattagtga agatttggag cctgaggcct ctgaaggcgg
     1141 tggagaaggc cctgagcctt gggagaccat ttcagaggaa atgaacatgg caggagattc
     1201 tctcagcctg gctctgcccc caccgcctgc ctccccagtg tcccggacca gcccccagga
     1261 gctgtcggaa gaagtgagcc gaaggttgca gatcactccg gactccaacg gggaacagtt
     1321 cagttctctg attcagagag agccctcgtc aaggcttcgg tcctgcagtg ttaccgacac
     1381 ggttgctgag caagctcacc ttccaccgcc cagcacccca actaggcgag cccgttcgtc
     1441 aactgtcacg ggtggtgagg aatccacgcc atcagtggcc tatgtacata ccacgccggg
     1501 cctgccttca ggctgggaag aaagaaagga tgcaaaggga cgcacatact atgtcaatca
     1561 taacaatcga accacaactt ggactcggcc aatcatgcag cttgcagaag acggtgcctc
     1621 cggatcagcc acaaacagta acaaccacct agtcgaaccc cagatccgcc ggccccgtag
     1681 cctcagctcg ccaacagtaa ctttatctgc cccactggag ggtgccaagg attcacccat
     1741 ccgccgtgcc gtgaaagata ccctttccaa tccacagtcc cctcagccat caccttacaa
     1801 ctcccccaaa ccacaacaca aagtcacaca gagcttcctg ccaccaggct gggagatgag
     1861 gatagccccc aacggccgac ccttcttcat tgaccataac acaaagacta caacctggga
     1921 agatccacgg ttgaaatttc cagtacacat gcggtcaaaa gcatctttaa accccaatga
     1981 cctgggccct cttcctcctg gctgggaaga gaggatccac ttggatggcc gcacgtttta
     2041 cattgaccat aatagtaaaa ttacccagtg ggaagatcca agactacaga acccagccat
     2101 cactggtccg gctgttccgt actccagaga gtttaagcag aaatacgact actttaggaa
     2161 gaaattaaag aagcctgctg atattccaaa caggtttgaa atgaaacttc acagaaacaa
     2221 catatttgaa gagtcctatc ggaggatcat gtctgtaaag agacctgacg tcctaaaggc
     2281 taggctgtgg attgagtttg aatcagagaa aggcctggac tatgggggcg tggccagaga
     2341 atggttcttc ttactgtcca aagagatgtt taacccctac tatggcctct tcgagtactc
     2401 tgccacggac aactacacac ttcagatcaa tcccaactca ggcctctgta atgaagacca
     2461 tttgtcctat ttcaccttca ttggaagagt tgctggccta gcggtgtttc atgggaaact
     2521 cttagatgga ttcttcattc gaccattcta caagatgatg ctggggaagc agataacgct
     2581 gaacgacatg gagtccgtgg acagcgagta ctacaactct ttgaagtgga tcttagaaaa
     2641 cgaccccacg gaacttgacc tcatgttctg catagacgaa gagaactttg ggcagacata
     2701 ccaagtggat ctgaagccca acgggtcaga aataatggta accaatgaga acaaacgaga
     2761 atacattgac ttagtcatcc agtggagatt tgtgaacagg gtccagaagc aaatgaatgc
     2821 cttcttggag ggatttacag aacttcttcc aatcgacttg attaaaattt ttgatgaaaa
     2881 tgagctggag ttgctgatgt gcggccttgg tgatgtcgac gtgaacgact ggagacagca
     2941 ctctatttac aagaacggct actgccccaa ccaccctgtc atccagtggt tctggaaggc
     3001 cgtgctcctg atggatgctg agaagcgcat ccggttacta cagtttgtca caggcacctc
     3061 cagagtaccc atgaatggat ttgccgaact ctatggttcc aatggtcctc agctgtttac
     3121 aatagagcaa tggggcagtc ccgaaaaact acccagagct catacatgct ttaatcgcct
     3181 tgatttacct ccatatgaaa cctttgaaga tttacgggag aaacttctca tggctgtgga
     3241 aaacgctcaa ggcttcgaag gtgtggatta aggaaggctc cagcgtccgg ggcactgccc
     3301 ttccagcaag ttccaagcgc acttttgcat ttgccgagca gacttttgca gagccggtgg
     3361 cagaagcagt ggcctggccc ggctctggag cccagcttgt ctgggccctg cccaagttcc
     3421 gccagggaac ccagccccag ctcaagtgcc gccccctcac cgccaattct caactggttg
     3481 ccgtgtacac tgattacatt tcaggaggac gtgttctatt tatgttgtgc ctctgcaggc
     3541 aaagccctta ataaatattt tgcatacttt ctaatgacaa tgaatggaat taatcacgct
     3601 acaggtatag tattacaact catgtttact ttttaaaatg atttagactg attttcagct
     3661 tttatttcat tacaattaaa gatgtctcat gtacttggaa aagtgagcat attttttttt
     3721 gtatttgaat ttcatatcaa gcttaatgtc agtgacattt ttttattttt gaagtacttt
     3781 gacactccca gcctctactt tattagaatt ggaagacgaa tttttcttca aaagccttct
     3841 acagacaaat acttttgaaa gaacttcaaa tataacatta agcataatat tttttccata
     3901 ctcagaatga aaataaactg gatattacct tttttttttt ttttgtacaa atttaaatac
     3961 taggtccagg ctggaagaat tgtaacatag catgatatct ttgtgttaac ttgaaaggac
     4021 tgaccccatt gtcccttaga agtactgacg tgtgccatca cgcatttaag acaggattgg
     4081 actgtcatct cgcatcagaa accgttacaa tttcctgttg ctgtacagcc tcttctgaac
     4141 tctcttctct ctttggtgtt catagagaca tttccaatgg cattgcttga ttcaaaacca
     4201 aggaaaccag aaccattgat cttctatctc tgtgattgct aagcagtagc caaataacct
     4261 tcctttgctg tatctgcgtc ccttctgaag gcactttatg gaggtcacat ctggttttgt
     4321 ttccattttt tttttttttt tcacgaatat cactcgtggc gattgctcct gcctccctgc
     4381 acaagtctat ccggtgcggt atctatcagg tgtgaacttg gctgctagcg tatgaaccca
     4441 gatgtgacgc agagcctctg cagcgatctt ctccaactgt tctgtaatct ctactcaaca
     4501 ataaggtttt agttactgta tacacttaac caagttgtgt gacctatgac ctactttgca
     4561 tgctctgata ctgtgtgccg cccccacccc caccccccag gtcgtgtctg ttgtgtgcta
     4621 gtttaaaagt gacctagaaa gcccagtagg aaccacagag ctcaagctgt ctggctcaag
     4681 tggtggaaga gtgagggagt ctgcagtttg taagtctcgt tctgttgtgt tctgagaaga
     4741 tgcctgaggc agtatgggag tttcaacacc cctgctgtcc ctttaacttg tattgtccta
     4801 gaccctgtcc tggaagttag aggacgtggg gtattttttt ttttgatctc ttgccagttg
     4861 tgaataagac agaaagactg tgttctctgt tacgtaagaa gggtctttgg tagaagaaat
     4921 tgctcgtgtt tacacagctg tggatgccat gaagaagccg tggacattct tggtgggtga
     4981 agccctagac gatggaatcc taccaagctg aacgtcggct tctatccata tgctatcgag
     5041 taattataga agcaattaac actcactgtt cttgcctctg gtgagatatt tttctcctgt
     5101 agttaacaga tattgggtgg ggggagggga gatacttttt ttttaataac cttccaagtc
     5161 cagaaccatc tctgttatag agtgtgccaa agcctgagct aagcagtgta ttttaatcga
     5221 agaagaaacc aaaatctgtt ctaagaactg gggttctata cctctgtagg ccctttattg
     5281 aacagcatta ttcactagat gcaaagggag ccagcaagat ggctcagtga gtctgggtgc
     5341 ttgtcaccta gcctgacatt ctgagttcaa tccctgggac ccgcctgata ggagagaact
     5401 gacttctaca agttgtcctc tgacccaata gatttacaaa caaacaaaca aaagattaaa
     5461 aataagaaaa gaggacttaa ttctgatctt ttctaagcca agagcagaaa agacttctta
     5521 aatccactcc agggaatact tgaaaggcaa gtcattgtga ctaacacttc agttcattgg
     5581 cccatgatca tttcagattt taacagctgt tactgttatt ggagatcgga gagttggcct
     5641 gttagaaatt aaaaaataca gtacagcttg ccgttcctct ggtttgtgaa gtttcactcg
     5701 ttgcctaaaa tgcccgatgc ctcgtctcca gaccttgctg cccttgcctg gtcatctttg
     5761 tacagtaatt gtaaatggca gtggagacta tggtgttcct gaggggcaat ggcaggtcac
     5821 tctcccgatc tccctactac cagccaggtg ctgtttcctt ctgagctgtg gagatcacac
     5881 catcctgtcg ccctgcaaat aattgtggat catttacctt gaggctgcag agtgggttcc
     5941 aggactctta cttcactgtg gagggacttc gagcatcagg tggctctctc atgttcctgc
     6001 cccctttact ccatgccaaa cctgggatta gacagcctgc tggctccaga aaaaaggtct
     6061 tggtaggcat tagtctatcc taggtgcccc cagtgcactg tccttgtctg tctgaagcac
     6121 ccatctctac cttccctggc cagtcctctg acggttcaca agggccagag atatggactt
     6181 tatttttgtt cccaggactg atgtagctta gtggcaagtg tctccctggc atgtacttag
     6241 ctgtagattt gatcctcatt gctgggactg gggacggggt ggattagaaa caaaaattct
     6301 gtttctgagg acttttagca agagacagaa aatcctgcca ttctggttgg aaattatata
     6361 aaccaacatg acaataactg tttcccccca aaaggctcag gagctgactt ttagaagtga
     6421 ggttcaaagt ctttaaccac ttctgaatct catatggcct gttgctcttg gtcttgctag
     6481 actcccgttt gttctaagag tcaggcttat gtatagcagc ttctagctgg ttctctatta
     6541 tcctggtaat attgtttctc agctctgaaa tttgaaagtc tacgaagaaa gcctgggttc
     6601 atcagctgcc cagccccacc tctccactga gcgctctctc tctctccatc tccctctctc
     6661 cctctttccc tctctccctc tcttcctccc tccctctctc cctctccccc tcaacagaag
     6721 gaaggctatc tgttgtcatt ctcttcctgg ccagtaggag tgctgctgtg cagggtcact
     6781 gcctgccctg ctccctctct ggctcccaga agatcacatg ctacctctgt ctcatttaca
     6841 aattggggta tatcctaagt catgtcaaac tgccgatttc aggactggga tgtgttttaa
     6901 aaaaaaaaaa gaaaaaacaa aaacaaaaaa cttaaactcc actttgtacc ttagatttta
     6961 aaactgggta gaaatgtaat atacattgga ccactttttt aagccttaat gaacagtgta
     7021 tatacatctg tgtatgcaca ctccacccca gagtgttttc agtgtgccag agaagcagct
     7081 gtagcgttag gacatgagcc tgtctagtgt ttcctaggaa agggatagtt ttctgttggt
     7141 atagcatttt ctaaggaaac tactcactca gcgcctaccc tttgttagaa gtgttgacgc
     7201 tccaagcacg cgtgtgaagc accatgtatc ttggccggtt tcatatttgt aagaagcacc
     7261 ggtaggaacc taagagcatc ggttggagtg tgacctctga tgcccctgca ttcccgccac
     7321 tgtgtctctg tgtattgaaa gcctctcctg tgaccacagc ctgtgttgac agatgtctgt
     7381 ctgtccctgt gcaatccagt ctgggcctca gactcagact gctgtatcta gcctacgttg
     7441 tcacaatttc cttctctgtt aactctgtga ctgttcttcc ttttgctact ctcgacgtaa
     7501 catatataca tatgtgtgtg tgtgtgtgtg tgtgtgtgtg ctatccaaat atatttgtat
     7561 atatttgtat agcattttta cactttagga gtaatctgga attcaaaagg gaatgagagc
     7621 ttactccctt ccacagtttc ctccaagccc tgtcctgaga attctgaccc tcgacacctg
     7681 agagaaccct agaaggacat tattagcttg ctgtcatttc tgaacccagg atctgtcatc
     7741 aaatgaaaca tgtttaaaaa gtgactctga aggtgaaaag agtttcaagc attccaaatg
     7801 aattctcagc gtttcttaac tttttattat gacccccctc cccgcccgcc acacacacac
     7861 acacacacac acacacacac acacacacac acacacacac ctcctaacgc aaagctgagt
     7921 gctatttgat acaatagtca aaaccaaact accttgacat ttttctaaga cagcttaatg
     7981 gctgatgctc gtcttcacag cccgtggcgg gctttaacgt agctcgaatc tggtacacgc
     8041 ataaagtttt acggtcaagt ccttttcttt cttcttgaga gacctaaaga gatactgtag
     8101 cttgtttttc tgatggcaaa tgggaactgt gacaaagtga agtccttgta aagcggtgat
     8161 acaacttgtc aaatatttaa taaagaatta cggaagctgg aa
//
