LOCUS       NM_025383               1920 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus NECAP endocytosis associated 2 (Necap2), transcript
            variant 1, mRNA.
ACCESSION   NM_025383
VERSION     NM_025383.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Mattera,R., Ritter,B., Sidhu,S.S., McPherson,P.S. and
            Bonifacino,J.S.
  TITLE     Definition of the consensus motif recognized by gamma-adaptin ear
            domains
  JOURNAL   J Biol Chem 279 (9), 8018-8028 (2004)
   PUBMED   14665628
REFERENCE   3  (bases 1 to 1920)
  AUTHORS   Ritter,B., Philie,J., Girard,M., Tung,E.C., Blondeau,F. and
            McPherson,P.S.
  TITLE     Identification of a family of endocytic proteins that define a new
            alpha-adaptin ear-binding motif
  JOURNAL   EMBO Rep 4 (11), 1089-1095 (2003)
   PUBMED   14555962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY159605.1, AK155871.1 and AI840582.1.
            
            On Sep 19, 2017 this sequence version replaced NM_025383.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037069.1, AK155871.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BY159605.1         1-25
            26-1523             AK155871.1         2-1499
            1524-1920           AI840582.1         1-397               c
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 73.35 cM"
     gene            1..1920
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /note="NECAP endocytosis associated 2"
                     /db_xref="GeneID:66147"
                     /db_xref="MGI:MGI:1913397"
     exon            1..145
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..854
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /note="likely ortholog of H. sapiens adaptin-ear-binding
                     coat-associated protein 2 (NECAP2); clathrin-coated
                     vesicle coat protein; NECAP endocytosis-associated protein
                     2"
                     /codon_start=1
                     /product="adaptin ear-binding coat-associated protein 2"
                     /protein_id="NP_079659.1"
                     /db_xref="CCDS:CCDS18862.1"
                     /db_xref="GeneID:66147"
                     /db_xref="MGI:MGI:1913397"
                     /translation="MEESEYESVLCVKPEVHVYRIPPRATNRGYRASEWQLDQPSWSG
                     RLRITAKGKVAYIKLEDRTSGELFAQAPVDQFPGTAVESVTDSSRYFVIRIEDGNGRR
                     AFIGLGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQNPDEGPKLDLGFKDGQTIKI
                     NIANMRKKEGAAGTPRARPTSAGGLSLLPPPPGGKSSTVIPPSGEQLSVGGSLVQPAV
                     VSGSGGATELWPQSKPAAAATADIWGDFTKSTGSPSSQSQPGTGWVQF"
     exon            146..246
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            247..351
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            352..433
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            434..542
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            543..720
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            721..805
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            806..1906
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1880..1885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /note="hexamer: AATAAA"
     polyA_site      1901
                     /gene="Necap2"
                     /gene_synonym="1110005F07Rik; NECAP-2"
                     /note="major polyA site"
ORIGIN      
        1 ggtcctgaag gggcggggag aagccggaag tcccgagggc tcgggcgata gagatggagg
       61 agagtgagta cgagtctgtt ctgtgtgtca agccggaggt ccacgtctac cgcatcccgc
      121 cgcgggccac caaccgtggt tacagggcct cagaatggca actggaccag ccatcgtgga
      181 gtggccggct gcggatcact gcgaaaggga aggtggccta catcaagctg gaggacagga
      241 cctctgggga gctcttcgca caggcccccg tggaccagtt tcccggcacc gcagtggaga
      301 gtgtgacaga ctccagcagg tacttcgtca tccgaattga agatggaaat gggcgacgtg
      361 cttttattgg gcttggcttt ggagaccgag gggacgcctt tgacttcaac gtggcattac
      421 aggaccattt caagtgggtg aagcagcagt gtgagtttgc gaaacaagcg cagaacccag
      481 atgaaggccc caagttggac ctgggcttca aggacggcca gaccatcaag atcaacatcg
      541 caaacatgag gaagaaggaa ggagcagctg gtaccccaag agccaggccc accagcgcag
      601 gagggctgag cctgctgccc cctcctccgg ggggcaagag ttccactgtc atccctccct
      661 ctggagagca gctgtctgtg gggggctctc tggttcagcc agcagttgtt tctggttcag
      721 gaggtgccac agaactctgg ccacaatcta agcctgctgc cgctgccact gctgatatct
      781 ggggagactt caccaaatcc acagggtccc cctccagcca gtctcagcca ggcaccggct
      841 gggtccagtt ctgacctgca caaccttttc ctcagccgac ttctgggaag gagctgcctc
      901 gtcctgaccc acggaaggtg gcagagtgct ccctgcctgg cccctggccc ctggcccctt
      961 gcctggctgg cttgacagcc tgagcacaag aagcagtaag ttggcacctt gccacactgt
     1021 tggttgggcc caggtgctgc tcagccagga aacgagagca aacactccag ggcctttgca
     1081 agcccatcgc cctcctcact ggagacaccc agacctgcct tcctccacca agagatcctt
     1141 attaaacaca gcccccaaag cacagaatgg attcaatttg tggcctgggt tcagctctgc
     1201 ctctgcagtt ggcaggtgtc ccagatttgc tgttggacag ggaccttctg cactccatcc
     1261 ataatagcct ttggtacctt ccagaggagt ccttcccgag agctggcaag tctttgagct
     1321 gtagcttgaa tgggctgtcg ggggacactg gaagggagta agcagctttt tgaaaatgtt
     1381 aatacattac tctgctaata tgctgacctg cactaatgca cccctgtgag ggacccccca
     1441 tggcgagggc ctctgcctct gacacaccct tctcgctgcc ctgagccccg gaggcagcca
     1501 tgggaggcag cctgcatacc tggaagccag ctgcacagtg ctgtggggaa acagaagcgt
     1561 tacagaccgg gccaggctgc ctggctgctg ctccctctgg gctcatagct ggcccacttg
     1621 acggtccctg gtggcttctg gagggaagct gtacctctag atacattttt cttttccttc
     1681 agagaccgtg acagagggag ctctgtcagc cggaagttca ggccaccaga cgtagtgatt
     1741 tgtaagaaat tactgtctcc tcagtgaacc tcctagtttc tttatagact taaccttccc
     1801 ttcagttaaa gggtttcttt ttcagagggc ctagactgtg ctgaccttgt gtgaccagag
     1861 atttaaagat gaaaaaaaca ataaatgtgt ttacttctgc accacaaaaa aaaaaaaaaa
//
