LOCUS       NM_026267               2408 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus NECAP endocytosis associated 1 (Necap1), transcript
            variant 1, mRNA.
ACCESSION   NM_026267
VERSION     NM_026267.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Alazami,A.M., Hijazi,H., Kentab,A.Y. and Alkuraya,F.S.
  TITLE     NECAP1 loss of function leads to a severe infantile epileptic
            encephalopathy
  JOURNAL   J Med Genet 51 (4), 224-228 (2014)
   PUBMED   24399846
  REMARK    GeneRIF: NECAP1 mutation links trafficking pathway in early
            infantile epileptic encephalopathy.
REFERENCE   3  (bases 1 to 2408)
  AUTHORS   Ritter,B., Murphy,S., Dokainish,H., Girard,M., Gudheti,M.V.,
            Kozlov,G., Halin,M., Philie,J., Jorgensen,E.M., Gehring,K. and
            McPherson,P.S.
  TITLE     NECAP 1 regulates AP-2 interactions to control vesicle size,
            number, and cargo during clathrin-mediated endocytosis
  JOURNAL   PLoS Biol 11 (10), e1001670 (2013)
   PUBMED   24130457
REFERENCE   4  (bases 1 to 2408)
  AUTHORS   Ritter,B., Denisov,A.Y., Philie,J., Allaire,P.D.,
            Legendre-Guillemin,V., Zylbergold,P., Gehring,K. and McPherson,P.S.
  TITLE     The NECAP PHear domain increases clathrin accessory protein binding
            potential
  JOURNAL   EMBO J 26 (18), 4066-4077 (2007)
   PUBMED   17762867
REFERENCE   5  (bases 1 to 2408)
  AUTHORS   Ritter,B., Denisov,A.Y., Philie,J., Deprez,C., Tung,E.C.,
            Gehring,K. and McPherson,P.S.
  TITLE     Two WXXF-based motifs in NECAPs define the specificity of accessory
            protein binding to AP-1 and AP-2
  JOURNAL   EMBO J 23 (19), 3701-3710 (2004)
   PUBMED   15359277
REFERENCE   6  (bases 1 to 2408)
  AUTHORS   Mattera,R., Ritter,B., Sidhu,S.S., McPherson,P.S. and
            Bonifacino,J.S.
  TITLE     Definition of the consensus motif recognized by gamma-adaptin ear
            domains
  JOURNAL   J Biol Chem 279 (9), 8018-8028 (2004)
   PUBMED   14665628
REFERENCE   7  (bases 1 to 2408)
  AUTHORS   Ritter,B., Philie,J., Girard,M., Tung,E.C., Blondeau,F. and
            McPherson,P.S.
  TITLE     Identification of a family of endocytic proteins that define a new
            alpha-adaptin ear-binding motif
  JOURNAL   EMBO Rep 4 (11), 1089-1095 (2003)
   PUBMED   14555962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK004805.1 and AW046891.1.
            
            On May 30, 2018 this sequence version replaced NM_026267.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011466.1, AK004805.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2393              AK004805.1         2-2394
            2394-2408           AW046891.1         1-15                c
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 57.96 cM"
     gene            1..2408
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /note="NECAP endocytosis associated 1"
                     /db_xref="GeneID:67602"
                     /db_xref="MGI:MGI:1914852"
     exon            1..107
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..840
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /note="brain enriched NECAP2 similar protein;
                     clathrin-coated vesicle coat protein; NECAP
                     endocytosis-associated protein 1; snoRNA MBI-129"
                     /codon_start=1
                     /product="adaptin ear-binding coat-associated protein 1"
                     /protein_id="NP_080543.2"
                     /db_xref="CCDS:CCDS20505.1"
                     /db_xref="GeneID:67602"
                     /db_xref="MGI:MGI:1914852"
                     /translation="MAAELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWT
                     GRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGR
                     SAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISKESQEMDNRPKLDLGFKEGQTIK
                     LSIGNITAKKGGASKPRASGTGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKS
                     NHGGNDSDILLDLDSPAPVSTSAPAPVSTSNDLWGDFSTASSSVPNQAPQPSNWVQF"
     exon            108..208
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            209..313
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            314..395
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            396..504
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            505..688
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            689..791
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            792..2395
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2374..2379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2395
                     /gene="Necap1"
                     /gene_synonym="1200016B17Rik"
                     /note="major polyA site"
ORIGIN      
        1 agcggagcca agatggcggc agagctggaa tatgagtccg tgctgtgtgt gaagcccgac
       61 gtcagcgtct accggattcc gccgcgggcc tccaaccgcg gttacagggc atctgactgg
      121 aagctagacc aacctgattg gactggtcgc ctgcgaatca cttcaaaagg gaagattgcc
      181 tacatcaaac tggaagacaa agtttcaggg gagctctttg ctcaggcccc cgtagaacag
      241 tatcctggga ttgctgtgga gactgtgaca gactccagcc gctactttgt gatcaggatc
      301 caggatggaa ccgggcgcag cgcgtttatt ggcatcggct tcacggatcg gggagatgcc
      361 ttcgacttta atgtttccct gcaagatcac ttcaagtggg taaagcagga aactgagatt
      421 tccaaagaat cacaggaaat ggataatcgt cccaagttgg atttaggctt caaggaagga
      481 cagaccatca aattgagtat tgggaatatt acagccaaga aaggaggtgc ttctaagccc
      541 cgggcctcag ggacgggggg cctgagctta ctgccaccgc cacctggagg caaagtcact
      601 attcccccac catcctcctc agttgccatc agcaaccatg tcaccccacc acctattcca
      661 aaatctaacc atggaggtaa tgattcagat atcctgttag atttggattc tccggctcct
      721 gtctcaactt cagcaccagc tccagtttct acaagcaatg acttgtgggg agacttcagc
      781 actgcatcca gctctgttcc taaccaggca ccacagccat ctaactgggt ccagttttga
      841 gtagcattgg caagaagtaa gaacacttga agaataaaaa tgacctcgag ggcaccattt
      901 tctgagggag ttgagggaca gcttcatttc ccagaaccca aatcagtggt cagtctttcc
      961 cgtagcttct ctgtgcattc aggctggatt cttttttgtt acctctgtgt acttgctgta
     1021 tagccagggg acaatctgct gtttcctgct cataaccaag caggggagta gtgggaatta
     1081 tcacactgac caactttgca gagttcagaa ggccaacttt atgagtggga gtgacctcta
     1141 acatacgtaa gttcttgaac ttatttcaga atcatctcat gattccctag ttagcaattt
     1201 caggagagac aaatgccttg aaactgtctt ataagactaa aaatgggcag gctggcccca
     1261 ggactcacaa agtttgggtt ttcatagggt tagatttgga gaaagctgtg tcccagttct
     1321 tttctgtaag gcctccttca ggcttatccc attcagtgag catcccgtgc tgggtggagc
     1381 cccatcacct tcttgtgtgt ttacatattt ttttcctttg acaagagggt tgtgttggtg
     1441 gtaccttact gttttcttgt tcaatagtgc agcatctttg accagtgaat atttctgaga
     1501 tgaagggagt caaggggctg tgctttccac attgtactcc acagaagtgt ttaatttctt
     1561 gcagctccat gtaattgctg catttgaagc atagcattaa tttatactca ccctgtgttt
     1621 gacctgaaga gttttaactt gatgtgtaga gcagaaagct ggaagcactt agctcccatt
     1681 cagtacccac aatgccttgc tgcctggctt tgactccttt tcataaacat ttcatttcag
     1741 tccatttagc gattcaatgg aaagctgctg ttaattatgt cagtgtgaag gaggttaagc
     1801 cacagctttc tttacctgtg acaggtaggc tttgcgctag gctttgatac ccagctagga
     1861 tatcttaaag caagttccca cccatcactc tccagtgtct gtccaccatt ccttttacag
     1921 tgctgtgaag agctcccgtt gaggttggtg ggtactgtcc tttgtttgtt gggcagtttg
     1981 agggcagaat gggaggactc tcactcctgc aagcacctgc ttggatagca cactctggct
     2041 gaagagggtc ctttcaaata tatagttcta ctcaatgctc tccttagctt tttctaactt
     2101 cgggtccatt ctttcccatt gcctcctccc cttcccaggc agctctcttc ttgcagagcc
     2161 atggcaggac atttaagttc cgatacaaac actaagaaga aagtataaga tcactagtga
     2221 ctgttgggaa acctattttc tcaatcttcc tccattttgt gttctttgta ttcttaagat
     2281 gataatatat tatgtatttg aattgctgaa aattgaaaat gaagttgaag atatatgtat
     2341 ataagcgtat gctgtattgg tgcaataatg gtaattaaag atatttaaaa aggtaaaaaa
     2401 aaaaaaaa
//
