LOCUS       NM_001362722            8972 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus nebulette (Nebl), transcript variant 2, mRNA.
ACCESSION   NM_001362722
VERSION     NM_001362722.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Montanes-Agudo,P., Aufiero,S., Schepers,E.N., van der Made,I.,
            Cocera-Ortega,L., Ernault,A.C., Richard,S., Kuster,D.W.D.,
            Christoffels,V.M., Pinto,Y.M. and Creemers,E.E.
  TITLE     The RNA-binding protein QKI governs a muscle-specific alternative
            splicing program that shapes the contractile function of
            cardiomyocytes
  JOURNAL   Cardiovasc Res 119 (5), 1161-1174 (2023)
   PUBMED   36627242
REFERENCE   3  (bases 1 to 8972)
  AUTHORS   Ye,Q., Bhojwani,A. and Hu,J.K.
  TITLE     Understanding the development of oral epithelial organs through
            single cell transcriptomic analysis
  JOURNAL   Development 149 (16) (2022)
   PUBMED   35831953
REFERENCE   4  (bases 1 to 8972)
  AUTHORS   Gan,P., Wang,Z., Morales,M.G., Zhang,Y., Bassel-Duby,R., Liu,N. and
            Olson,E.N.
  TITLE     RBPMS is an RNA-binding protein that mediates cardiomyocyte
            binucleation and cardiovascular development
  JOURNAL   Dev Cell 57 (8), 959-973 (2022)
   PUBMED   35472321
REFERENCE   5  (bases 1 to 8972)
  AUTHORS   Vejandla,R.M., Orgil,B.O., Alberson,N.R., Li,N., Munkhsaikhan,U.,
            Khuchua,Z., Martherus,R., Azeloglu,E.U., Xu,F., Lu,L., Towbin,J.A.
            and Purevjav,E.
  TITLE     Deficiency in nebulin repeats of sarcomeric nebulette is
            detrimental for cardiomyocyte tolerance to exercise and
            biomechanical stress
  JOURNAL   Am J Physiol Heart Circ Physiol 320 (5), H2130-H2146 (2021)
   PUBMED   33861145
  REMARK    GeneRIF: Deficiency in nebulin repeats of sarcomeric nebulette is
            detrimental for cardiomyocyte tolerance to exercise and
            biomechanical stress.
REFERENCE   6  (bases 1 to 8972)
  AUTHORS   Maiellaro-Rafferty,K., Wansapura,J.P., Mendsaikhan,U., Osinska,H.,
            James,J.F., Taylor,M.D., Robbins,J., Kranias,E.G., Towbin,J.A. and
            Purevjav,E.
  TITLE     Altered regional cardiac wall mechanics are associated with
            differential cardiomyocyte calcium handling due to nebulette
            mutations in preclinical inherited dilated cardiomyopathy
  JOURNAL   J Mol Cell Cardiol 60, 151-160 (2013)
   PUBMED   23632046
  REMARK    GeneRIF: This study showed distinct effects of NEBL mutations on
            sarcomere ultrastructure, cellular contractile function, and
            calcium homeostasis in preclinical DCM in vivo.
REFERENCE   7  (bases 1 to 8972)
  AUTHORS   Oeschger,F.M., Wang,W.Z., Lee,S., Garcia-Moreno,F., Goffinet,A.M.,
            Arbones,M.L., Rakic,S. and Molnar,Z.
  TITLE     Gene expression analysis of the embryonic subplate
  JOURNAL   Cereb Cortex 22 (6), 1343-1359 (2012)
   PUBMED   21862448
REFERENCE   8  (bases 1 to 8972)
  AUTHORS   Nakagawa,H., Suzuki,H., Machida,S., Suzuki,J., Ohashi,K., Jin,M.,
            Miyamoto,S. and Terasaki,A.G.
  TITLE     Contribution of the LIM domain and nebulin-repeats to the
            interaction of Lasp-2 with actin filaments and focal adhesions
  JOURNAL   PLoS One 4 (10), e7530 (2009)
   PUBMED   19851499
  REMARK    GeneRIF: Actin filament binding activity may be a major contributor
            to the subcellular localization of lasp-2 to filopodia but is not
            crucial for lasp-2 recruitment to focal adhesions [lasp-2]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8972)
  AUTHORS   Li,B., Zhuang,L. and Trueb,B.
  TITLE     Zyxin interacts with the SH3 domains of the cytoskeletal proteins
            LIM-nebulette and Lasp-1
  JOURNAL   J Biol Chem 279 (19), 20401-20410 (2004)
   PUBMED   15004028
  REMARK    GeneRIF: LIM-nebulette, Lasp-1, and zyxin may play an important
            role in the organization of focal adhesions [LIM-nebulette]
REFERENCE   10 (bases 1 to 8972)
  AUTHORS   Villanueva,M.P., Aiyer,A.R., Muller,S., Pletcher,M.T., Liu,X.,
            Emanuel,B., Srivastava,D. and Reeves,R.H.
  TITLE     Genetic and comparative mapping of genes dysregulated in mouse
            hearts lacking the Hand2 transcription factor gene
  JOURNAL   Genomics 80 (6), 593-600 (2002)
   PUBMED   12504851
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772218.29 and AL935297.7.
            
            Transcript Variant: This variant (2) differs in the 5' coding
            region and 5' UTR and contains multiple additional coding exons
            compared to variant 1. The encoded isoform (2) is longer than
            isoform 1 and contains multiple nebulin repeats and an SH3 domain.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849378, SAMN00849379
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-190               AL772218.29        117759-117948       c
            191-278             AL772218.29        115550-115637       c
            279-350             AL772218.29        115377-115448       c
            351-455             AL772218.29        107582-107686       c
            456-566             AL772218.29        105292-105402       c
            567-677             AL772218.29        98807-98917         c
            678-779             AL772218.29        89971-90072         c
            780-881             AL772218.29        89008-89109         c
            882-995             AL772218.29        79613-79726         c
            996-1100            AL772218.29        68775-68879         c
            1101-1208           AL772218.29        66178-66285         c
            1209-1319           AL772218.29        59256-59366         c
            1320-1430           AL772218.29        55566-55676         c
            1431-1541           AL772218.29        52149-52259         c
            1542-1652           AL772218.29        48258-48368         c
            1653-1763           AL772218.29        48010-48120         c
            1764-1868           AL772218.29        45904-46008         c
            1869-1961           AL772218.29        43784-43876         c
            1962-2054           AL772218.29        34842-34934         c
            2055-2147           AL772218.29        31715-31807         c
            2148-2252           AL772218.29        30308-30412         c
            2253-2424           AL772218.29        29277-29448         c
            2425-2517           AL772218.29        25551-25643         c
            2518-2667           AL772218.29        24621-24770         c
            2668-2774           AL772218.29        5797-5903           c
            2775-4736           AL772218.29        2001-3962           c
            4737-8972           AL935297.7         139960-144195       c
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 12.43 cM"
     gene            1..8972
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="nebulette"
                     /db_xref="GeneID:74103"
                     /db_xref="MGI:MGI:1921353"
     exon            1..190
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            191..278
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..2951
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="isoform 2 is encoded by transcript variant 2;
                     actin-binding Z-disk protein"
                     /codon_start=1
                     /product="nebulette isoform 2"
                     /protein_id="NP_001349651.1"
                     /db_xref="GeneID:74103"
                     /db_xref="MGI:MGI:1921353"
                     /translation="MKVPVSGDVKEETEEENVEQEENQEAKVSLKPVIEDLSMELARK
                     CTELISDIHYKEEYKKSKDKCTFVTDTPMLNHVKNIGAFISEAKYKGTIKADLSNCLY
                     KDMPATIDSVFAREVSQLQSEVAYKQKHEAEKGFSDYTHMKEPPEVRRAMEVNRHQSN
                     ISYRKDMQGTHTYTAELDRPDIKKATQISKIISDAEYKKGQGIVNKEPSVIGRPDFEH
                     AVGASKLSSQVKYKEKFDNEMKEKSHHYNPLGSAFFRQHQFAAVLASDWEYKRDFEEN
                     KGLYHFDAEAPEHLHHKGNATLQSQVKYREEYEKNKGKSMLEFVETPSYQSSKEAQKM
                     QSEKVYKEDFEKEIKGRSSLDLDKTPAFLHVKHITNLMREKEYKKDLENEIKGKGMEL
                     SSEVLDIQRAKRASEMASEKEYKKDLELEIKGKGMQIDTDTLEIQRAKRAAKIASAKD
                     YKRDLETEIKGKGMQVSTDTLDVQRAKRASEMASQKQYKKDLENEIKGRGMQVNMDIP
                     DMLRAKRASEIYSQRKYKDEAEKMLSNYSTVAVTPEIQRIKTTQQNISNVSYKEEVGA
                     GTAVRNTPEIERVKKNQHNISSFQYKEQTYKATPVTMTPEIERVKRNQEQLSAVYYKG
                     QLGRATALSVTPEMERVKKNQENISSVKYTQDHKQMKGRPCLILDTPALRHVKEAQNN
                     VSMVKYHEDFEKTKGRGFTPVVDDPVTERVRKSTQVVSDAAYKGVQPHVVEMDRRPGI
                     IVDLKVWRTDPGSIFDIDPLEDNIQSRSLHMLSEKASQYRRRLSRSHSGSTFGTGLGD
                     DKSEISELYPSFSCYSEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSMRSMQPP
                     AHLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYIE
                     FVN"
     exon            279..350
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            351..455
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            456..566
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            567..677
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            678..779
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            780..881
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            882..995
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1100
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1208
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1319
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1430
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1541
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1652
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1763
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1868
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..1961
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2054
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2147
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2252
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2424
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2517
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2667
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2774
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     exon            2775..8972
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7574..7579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="hexamer: AATATA"
     polyA_site      7591
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="major polyA site"
     regulatory      8047..8052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="hexamer: AATAAA"
     polyA_site      8069
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="major polyA site"
     regulatory      8593..8598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="hexamer: AATAAA"
     polyA_site      8623
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
     regulatory      8730..8735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
                     /note="hexamer: AGTAAA"
     polyA_site      8748
                     /gene="Nebl"
                     /gene_synonym="1200007O21Rik; A630080F05Rik;
                     D830029A09Rik; Lnebl"
ORIGIN      
        1 cccagcgaag aaccattaag aactcacagc aagattgaag atgagaagaa atcctgctta
       61 agcctcaggc acgccattca ctatggaaac agacatcacc caaagcaaca tgagcattgt
      121 catctgaagt ggaagctaag aggttcatct tggacagcgt tgggatattt tttcctatgt
      181 acttctgaag ggtaaagatg aaggtacctg tatccgggga tgtaaaagag gaaactgagg
      241 aggaaaatgt ggagcaggaa gaaaaccagg aagccaaggt gtctctgaaa cctgttattg
      301 aagacctcag catggaactg gccagaaagt gcaccgagct cattagtgac atccattata
      361 aagaagagta caaaaagtct aaggacaagt gtacgtttgt gactgatacc cctatgctaa
      421 accatgtaaa aaatattggt gcttttattt ctgaggcaaa atacaaaggt accattaagg
      481 ctgatctttc caattgtctg tataaggaca tgcctgccac catcgacagt gtctttgcaa
      541 gagaagtatc ccagcttcag agtgaggttg cctacaagca gaagcacgag gctgagaaag
      601 gcttctccga ttacacccac atgaaggaac caccggaggt cagacgtgcc atggaggtca
      661 acagacacca gagtaatatt tcttacagaa aagatatgca aggcacccac acgtatacag
      721 cggagctgga cagaccggat atcaagaagg caactcagat ctccaagatc ataagcgatg
      781 cagaatacaa gaaagggcaa ggcatagtga ataaagaacc ttctgtaatt ggaagaccag
      841 attttgaaca tgctgtggga gcttctaaac tttctagtca ggttaaatat aaagaaaaat
      901 ttgataatga aatgaaggaa aaaagccacc attacaaccc tctgggaagt gcgtttttta
      961 ggcaacacca gttcgctgct gtcctggcca gtgattggga atataaaagg gattttgaag
     1021 aaaacaaagg attgtatcac tttgatgcgg aggcccctga acacctgcac cataaaggaa
     1081 acgccaccct ccagagtcag gtaaaataca gagaagaata tgagaaaaat aagggaaagt
     1141 ccatgcttga gtttgttgag acaccatcat atcagtcttc aaaggaagct cagaagatgc
     1201 agagtgagaa agtttacaaa gaagattttg agaaggaaat taaaggaagg tcctcactgg
     1261 atttagacaa gacacctgca tttctacatg tgaagcatat caccaacctg atgagggaga
     1321 aagaatataa aaaagatctg gaaaatgaaa tcaaagggaa aggaatggag ctcagttcag
     1381 aagttcttga tattcagaga gcaaagcgag catctgaaat ggccagcgag aaagaataca
     1441 aaaaggatct ggagttggaa attaaaggca agggaatgca aattgacacc gatacccttg
     1501 aaatacaacg tgccaaaaga gctgccaaga tagccagcgc gaaagactac aaaagagatc
     1561 tggagacaga aattaaagga aaagggatgc aagtcagcac agacactctg gatgtccaga
     1621 gagctaagag ggcatccgag atggccagcc agaagcaata taagaaagac ttggagaatg
     1681 aaattaaggg gagaggaatg caagtgaaca tggatatccc agatatgctt cgagccaaga
     1741 gggcatctga aatctacagc cagagaaagt ataaagatga agctgagaaa atgctttcca
     1801 actactccac tgtggcagtc actcccgaga ttcagagaat caagacgaca cagcaaaata
     1861 ttagcaatgt atcttataag gaagaagtgg gagctggcac agccgtaagg aataccccag
     1921 agattgaacg agtgaagaaa aaccagcaca atattagctc attccagtat aaagagcaaa
     1981 cctataaggc cactcctgta accatgaccc cagagataga gagagtgaag aggaaccagg
     2041 aacagctgag tgcggtttat tataaaggtc aactgggaag ggctacagct ttgagtgtaa
     2101 ctcctgaaat ggaaagagta aagaagaatc aagaaaatat cagctcggtg aaatacactc
     2161 aggaccataa acaaatgaaa ggtagaccgt gtctgatctt agatacaccg gctttgagac
     2221 atgttaaaga agcacaaaat aatgtttcca tggtcaaata ccatgaagat tttgagaaga
     2281 caaagggaag aggcttcact cccgttgtgg atgatcctgt gactgaacgg gtgaggaaga
     2341 gcacccaggt tgtgagcgat gctgcctata aaggggtcca gccacatgtt gtggagatgg
     2401 acagaagacc tgggatcatt gttgacctta aagtttggcg tacagatcct ggctccatct
     2461 tcgacattga ccccctggag gacaatattc agtctcgaag tctccatatg ctctctgaaa
     2521 aggcaagtca gtaccggaga cgcctgtctc gctcacattc cggcagcaca tttggtacag
     2581 gtctgggaga tgacaagtca gagatctctg agctgtaccc tagcttttca tgctacagtg
     2641 aggtaacaag accatctgac gaaggagcac ctgtccttcc tggagcttac cagcagagcc
     2701 attcccaagg ctatgggtac atgcatcaga ccagcgtgtc gtccatgagg tcaatgcagc
     2761 ctccagcaca tctaaggacc taccgcgcca tgtacgatta tagcgcccag gatgaagatg
     2821 aggtctcctt ccgagatggt gactacattg tcaacgtgca gcccattgat gatggctgga
     2881 tgtatggcac cgtgcagaga accgggagaa cgggcatgct cccagcaaat tacattgagt
     2941 ttgtgaatta attctttctc cttgcccttc gagctttatt atgatgtatt ccaaactcaa
     3001 tctttttaaa agatagatga tacttttcag ataacttggc aattatttta caataatgta
     3061 tccttacttt gacaattaga cacacaggta ccaggaaaaa aggaattgac tttcaggctg
     3121 gaaatggcag catccatagt aattcctgta aacaaaacca ttaggtctgg agacctggtg
     3181 ctgctaattg tgtttgtggt ttcattcaat tggctatcca acccttctgg gaaatgtatt
     3241 tttgtagact ttaatagaga tgttgattgt cccttaagtg taacaaatgt atgaaatttc
     3301 ttagctgtca cttggaatca tccatagcta attctcatag tccagcaaca catccaataa
     3361 tttaaaaact gttcagcttt taaatcattg gataattttc aattcaaggg aggatcacct
     3421 aatataataa ataaatggaa acagtggcag aatgggtctg gttaaaatca tattgactgt
     3481 agccttaggg agtcggcagg gaacttagcc tttccctagg ctgagcggga gctatttagt
     3541 tctcatgtgc ttcatggtgt agcctatctc catttatgtt ctgtggagca gtgcatctgc
     3601 tcaggtcccc agaatgtcag gaccaggtaa cttcccagct tctctgcaaa gggcaaagtc
     3661 taatgtcatc tgtactattt caccagtagc ctctatcctg ttgcatgtct gtgggcgtga
     3721 gcttctgtaa catggcaaca tctgtaacag ctgctagccc atccttgtta ctaaagtcaa
     3781 ccgtatggcc actctctccc tgcccttctt ctagttgccc gctcattgga aaacattttg
     3841 aaaagttgct tgaggctttc ttgctgcatt acactctagt tttgaaagac tcacacctta
     3901 ggaactacat atatactcta gtaaatagga gacttatgta tttactggat agctttcact
     3961 cacttaatgc caccattgat ttaaaagtgg agtgaattga acagcctcga ccgtaagcgg
     4021 aaatggagta taaagtagct gaaaaccaaa aacacagaac agcaaaggtt tgctcacagt
     4081 tagacaatct ggtcttatac tttggtaatc agtcttcaga tgctgggata gaaatctggc
     4141 cattaaatat gttcataaaa aggcttccct tttccattcc cgtttttgtt ttctaagtca
     4201 ccttcacgat tagattaaaa aaagtcatga gtgatttttt ggtggggagg gatgtccaac
     4261 agataatcca ctaaggaaga aatcagtgtc cggttttgag tagtttgcag gactcacaca
     4321 cttgtgagta aacttgtctg gaatctgcag ttgaagtcac ctggatgttg acatttattg
     4381 caactctgtt ttttcatagt gcctctgact gtgacttgca gcttggtggc attcttgtaa
     4441 gctctgaaaa tgaagagagt aaagtcacaa aaatataaga ggatactgcg actgtgggaa
     4501 tatctacttt acacaatatg cttaaagaaa taaacaaagc atccacaaca aaaaccattc
     4561 aaatgaaatg tttataaggc caaagtccaa agtgggttca tgtaaatctt tagaaagtga
     4621 gcccatactt ttgaaaaata ttagttttct tagatttgct ttaaggaaaa gttttgtcac
     4681 actaagctgt atttctgtga cacagataga aaattgaaaa ggaaaattct gcagataact
     4741 agagattttt ctggagaggt tgtttttata gactcataag taagtagctt acctaatctt
     4801 cacgtaattt aagtaaccaa tcattattaa ctttcctaac tgctttcatg tcttgaacac
     4861 acacctacaa agataaactg cacttgcaat aatttgcaaa aatctgtacc cgaaaaacaa
     4921 gggtgataag ttgcttcatt tatgtgatat gtcttttatg cttccagagg cagaccattt
     4981 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtttgt gactattttt caaaagtagt
     5041 caaaattaaa atgttaggat aaatgcaggg caggtatgat tttgatcata gtatatgtgt
     5101 aggaatctca acagaagcca actttgaaat gatgcttaac atttgcttgt ccccacaaag
     5161 tagtgactct atggtccatg tttttgccac tcattaattt caaagtcaga cttgatgagt
     5221 gtgagagtca gtacatattt aggagatagc cgttctaaat attgagcttt gcataatgtt
     5281 tgcttagcaa gatatttctg tttcaagtct ttcagaatta agatatgatc tggagctggc
     5341 agctgtggct cataccttta atcccagcac tcgggaggca gaggcaggca gatttctgag
     5401 ttcgaggcca gccaggtcta cagagtgagt tccaggacag ccagttttcc atacagacca
     5461 aggcagagca atatatggca tgctttcgag attatgcgtc gagcctagat gtgtcatagc
     5521 caggtgccat cttttgcagc atctttttaa gaaatactat attcttagca cctttttcgc
     5581 tttccacatg cctccctccc acgtgcagtt tggcagtcaa tatgtcacat gtaacattga
     5641 cagcaataga agaatttaag aacaagcccc ttagaaattg gagtcaaagg agactaaatg
     5701 ccaagcacag agggcacgca cctcttccta tctgatggat tccaagcttg ccaggagcac
     5761 aagaaggtac ttgatacact tctcaaagtc tctgaaaaca aatgcctttg gttcatagtc
     5821 ctcttgagaa aagtttagaa acttgatata atgcttttgc tataagcttt tgtagtagat
     5881 ttaagaaaaa aagcatttgt tatctagaaa gctttttctt tctagcctgt gacaaaatat
     5941 cttcctatct tcattttgct tgcacatctg cagcacagcc cagtttgcaa ctttgatctg
     6001 gttttcattt tccatggagg ctctgcctct tcccatgtct cccagcctcc acacagcacc
     6061 caggaatgtc taagcacaat ttctacaagc atcttgtaag ggggatggat gagattagtc
     6121 cagtttctct cttgctacta ggtcctttgc ttgaagatga tgaagatgtt tttagttagg
     6181 gatcttctct ctgctggagg cctataacac tataggaatt aggggaaaac cttttccata
     6241 attgagagca agtgacacac ctacttttca ggttcatcca cctccagtgg aatgttttca
     6301 gtcaaatgag aaagaaaata attgatcttt aataatacag ccctatattc agtagatgca
     6361 gtttatacta aggttacaat ataaatatat acaaaatagg tgcctttttg attatgcttg
     6421 tttatcatta tggtcttaga aaggaaacca agcaagcaag caagcttctg catattctat
     6481 agtaatattt tatgatacat gatcaatatt tttgtggtga gaagtgggat acccttgaaa
     6541 gaaagatact acaggtggta gctagttgta ttttccttct aaaggtttat aatactagaa
     6601 aatgctgact tattcaattt tctgagaagt ttttttgtgg atttatatat agttgagata
     6661 tataggcatt aagtttaggt ttggggttta aaataattat ggtgagacag actagctata
     6721 ttatgagact gaattcactg cataattgaa ctttatgacc tgattatatt ttaactgttt
     6781 gcctatgata atctgtttgt ggcttgtgtt gcgctgataa tctaaattat cattgcattg
     6841 tatggggtaa taaaacatga tgatagagct tcagttaagc aggaagctat gtaaaccttt
     6901 aaagatgcta aaaatgaagg tctttctgaa gtattccatg ctccttagga cccttctcca
     6961 ggcaagtagc tgtcaataac tgatgaccaa aaaagtcaag ctgtgtgaaa cttctgtgat
     7021 catcattcca ggatgtgtaa gaccactaga atccttatgc ctggaaactt ctctgagcaa
     7081 acgtccttcc caattatctg taacttcagt gtatctacaa gagcttgcca tgcttattgt
     7141 gtctggtgta ttgtatcctg gttgcatagc acacacttca ccgttgtcaa ttattgattg
     7201 ttaaagatga tttcctttaa aggaaagaga aaactgaatc gcagcatcct tccttcctgt
     7261 ccatacaatg aattgctgag cattttggaa gttgcaacaa gtgtgtaaca ggcatgatat
     7321 ttgtagaact gttggtttct gacctgtgat aaaaacacaa atttgaatga tgtagtttgg
     7381 cttctgtttg ctttgaaata tgcacagttg tggtttgtgg ttaatttgta aaattgtact
     7441 aactttgaga acataatgat tttgaaagaa gcattaaatg ttttttcccc caatggaagg
     7501 gtatcacaga atgatataag gttattagta taatttggca tttgttatgc aagttgtgaa
     7561 caccagctat taaaatatat tttagtagaa atgctttaat tcatgttgtt ttcctctaca
     7621 ctgtgaatct taagccttgg tggaccagag acacattgtg atgtccaaag gactaaccta
     7681 tgcaaatgag ttcacatctt atattgatat cacaataaac atgtgataag gcattatttc
     7741 tcctcataat gtacagcagt attgaaataa caaaggaagc ccagcctccc atatattaca
     7801 ctgtagtccc tcacaatccc ttcaaggcta ccgaaatcat tagcatttat gtaaatcaga
     7861 ctgatatcaa ctaaagcgac ttgaatgatt ctggacagaa aatgatggac aagcatctcg
     7921 actggatttc atagaactat gttctcacat actctctgtt cagggtcttg gtattctatg
     7981 tcttctatct gtatttgtaa agccttatga tgaattgatt tcctatcaac acactcagtt
     8041 tgcttaaata aattcaggat gtaacacaat ggttctacat ctttgctgtc acgtggttcc
     8101 tagaccaggc cattggatgc ttgcagcaca gtgggcattc ctactgttgg ctcacatgca
     8161 ggcatagcaa aggctcatga agacatagtg tatttatttt taagtgctat ggagaatgtg
     8221 tttttcccca tgaaactaac acacatttaa gaaataatta tatacttttt aatcccacaa
     8281 tctttctgat gtttataccc atgtattgct gggacacatc ctcgtagcta actttttttc
     8341 aaaactgaag tcttccctgg cctggtgtta gttttcttgt atttgaaaga tgaaagtagc
     8401 aacagaagca acttggagga aggaaagggc acggattgat actctgatct ttcatcaaag
     8461 aatgtctatc atagaacata attgcttctt ttctcatgtt tctgtatcac agaacgtaat
     8521 tgcttctttt ctcatggttt tctcataact gaaaagacag gtagttccaa tttttaccct
     8581 catctacact tcaataaatt ggatcttggc tttattgtgt gaatctctgg cataatcttt
     8641 ccaaactata aactgataaa tcttgtagac tgacacacac atttcaaaca ggtcaatgga
     8701 gaagcttagc aaatggcatt tgctaataga gtaaacactt ctaccaaatc ttgattgttc
     8761 tgttttttta tattctgtaa gtttgggttg aatatctgtg gtattatgaa tcaaacacac
     8821 tcatgatttt tgaaagtata aagtaaacaa cacaggtatt tattcatgtg aagaacttac
     8881 tatcccttcc ttcttccacg actttctttc atttcatgac tataactgta ccctttgaaa
     8941 tctgagccat aaagaaaatg aataattgac ct
//
