LOCUS       NM_028388               1540 bp    mRNA    linear   ROD 18-NOV-2024
DEFINITION  Mus musculus NADH:ubiquinone oxidoreductase core subunit V2
            (Ndufv2), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_028388 XM_001004994 XM_128725 XM_896886 XM_917758 XM_925211
            XM_981927 XM_981966
VERSION     NM_028388.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Vercellino,I. and Sazanov,L.A.
  TITLE     SCAF1 drives the compositional diversity of mammalian respirasomes
  JOURNAL   Nat Struct Mol Biol 31 (7), 1061-1071 (2024)
   PUBMED   38575788
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1540)
  AUTHORS   Chella Krishnan,K., Vergnes,L., Acin-Perez,R., Stiles,L., Shum,M.,
            Ma,L., Mouisel,E., Pan,C., Moore,T.M., Peterfy,M., Romanoski,C.E.,
            Reue,K., Bjorkegren,J.L.M., Laakso,M., Liesa,M. and Lusis,A.J.
  TITLE     Sex-specific genetic regulation of adipose mitochondria and
            metabolic syndrome by Ndufv2
  JOURNAL   Nat Metab 3 (11), 1552-1568 (2021)
   PUBMED   34697471
  REMARK    GeneRIF: Sex-specific genetic regulation of adipose mitochondria
            and metabolic syndrome by Ndufv2.
REFERENCE   4  (bases 1 to 1540)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1540)
  AUTHORS   Agip,A.A., Blaza,J.N., Bridges,H.R., Viscomi,C., Rawson,S.,
            Muench,S.P. and Hirst,J.
  TITLE     Cryo-EM structures of complex I from mouse heart mitochondria in
            two biochemically defined states
  JOURNAL   Nat Struct Mol Biol 25 (7), 548-556 (2018)
   PUBMED   29915388
REFERENCE   6  (bases 1 to 1540)
  AUTHORS   Dieteren,C.E., Koopman,W.J., Swarts,H.G., Peters,J.G., Maczuga,P.,
            van Gemst,J.J., Masereeuw,R., Smeitink,J.A., Nijtmans,L.G. and
            Willems,P.H.
  TITLE     Subunit-specific incorporation efficiency and kinetics in
            mitochondrial complex I homeostasis
  JOURNAL   J Biol Chem 287 (50), 41851-41860 (2012)
   PUBMED   23038253
  REMARK    GeneRIF: The authors use green fluorescent-tagged complex I
            subunits to determine the kinetics of assembly. Tagged human NDUFV2
            can replace the endogenous protein in the holo-complex I protein
            and was also found in complex I subassemblies, suggesting that
            NDUFV2 may directly incorporate into the holo-enzyme and may also
            assemble into smaller complexes or in a monomer pool.
REFERENCE   7  (bases 1 to 1540)
  AUTHORS   Hoefs,S.J., Rodenburg,R.J., Smeitink,J.A. and van den Heuvel,L.P.
  TITLE     Molecular base of biochemical complex I deficiency
  JOURNAL   Mitochondrion 12 (5), 520-532 (2012)
   PUBMED   22820119
  REMARK    GeneRIF: The authors provide an overview of the function and
            assembly of human complex I, also known as NADH dehydrogenase
            (ubiquinone) 1 alpha subcomplex. They discuss its role in oxidative
            phosphorylation and the implications of complex I deficiency in
            disease.
            Review article
REFERENCE   8  (bases 1 to 1540)
  AUTHORS   Wirtz,S. and Schuelke,M.
  TITLE     Region-specific expression of mitochondrial complex I genes during
            murine brain development
  JOURNAL   PLoS One 6 (4), e18897 (2011)
   PUBMED   21556144
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1540)
  AUTHORS   Werner,H.B., Kuhlmann,K., Shen,S., Uecker,M., Schardt,A.,
            Dimova,K., Orfaniotou,F., Dhaunchak,A., Brinkmann,B.G., Mobius,W.,
            Guarente,L., Casaccia-Bonnefil,P., Jahn,O. and Nave,K.A.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   10 (bases 1 to 1540)
  AUTHORS   Ragan,C.I., Galante,Y.M., Hatefi,Y. and Ohnishi,T.
  TITLE     Resolution of mitochondrial NADH dehydrogenase and isolation of two
            iron-sulfur proteins
  JOURNAL   Biochemistry 21 (3), 590-594 (1982)
   PUBMED   6279147
  REMARK    GeneRIF: The authors biochemically separate the NADH dehydrogenase
            module from complex I of bovine heart into subunit I, II and III
            fractions. Subunit II fraction, also known as the NADH
            dehydrogenase (ubiquinone) flavoprotein 2 protein, has a molecular
            weight of 24 000 Da and contains an iron-sulfur center.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY246389.1 and AK146998.1.
            
            On Jun 5, 2013 this sequence version replaced NM_028388.2.
            
            Summary: This gene encodes a subunit of the NADH-ubiquinone
            oxidoreductase (complex I) enzyme, which is a large, multimeric
            protein. It is the first enzyme complex in the mitochondrial
            electron transport chain and catalyzes the transfer of electrons
            from NADH to the electron acceptor ubiquinone. The proton gradient
            created by electron transfer drives the conversion of ADP to ATP.
            This gene is a core subunit and is conserved in prokaryotes and
            eukaryotes. The bovine ortholog of this protein has been
            characterized and is reported to contain an iron-sulfur cluster,
            which may be involved in electron transfer. In humans mutations in
            this gene are implicated in Parkinson's disease, bipolar disorder,
            schizophrenia, and have been found in one case of early onset
            hypertrophic cardiomyopathy and encephalopathy. A pseudogene of
            this gene is located on chromosome 3. Alternative splicing results
            in multiple transcript variants encoding different isoforms.
            [provided by RefSeq, Jun 2013].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146998.1, SRR1660811.308943.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 23038253; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                BY246389.1         2-69
            69-1540             AK146998.1         3-1474
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 35.26 cM"
     gene            1..1540
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="NADH:ubiquinone oxidoreductase core subunit V2"
                     /db_xref="GeneID:72900"
                     /db_xref="MGI:MGI:1920150"
     exon            1..145
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..841
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /EC_number="7.1.1.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; NADH dehydrogenase [ubiquinone] flavoprotein 2,
                     mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa
                     subunit; NADH dehydrogenase (ubiquinone) flavoprotein 2"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] flavoprotein 2,
                     mitochondrial isoform 1 precursor"
                     /protein_id="NP_082664.1"
                     /db_xref="CCDS:CCDS37679.1"
                     /db_xref="GeneID:72900"
                     /db_xref="MGI:MGI:1920150"
                     /translation="MFSLALRARATGLAAQWGRHARNLHKTAVHNGAGGALFVHRDTP
                     ENNPDTPFDFTPENYKRIEAIVKNYPEGHQAAAVLPVLDLAQRQNGWLPISAMNKVAE
                     VLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRDSDSILETLQRKLGIKVG
                     ETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTPKDIEEIIDELKAGKVPKPGPR
                     SGRFCCEPAGGLTSLTEPPKGPGFGVQAGL"
     exon            146..211
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            212..274
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            275..391
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            392..560
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            561..670
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            671..747
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            748..1540
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      875..880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      897
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="major polyA site"
     regulatory      1094..1099
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="hexamer: AGTAAA"
     regulatory      1117..1122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="hexamer: AATACA"
     polyA_site      1118
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
     polyA_site      1141
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
     regulatory      1419..1424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
                     /note="hexamer: AAGAAA"
     polyA_site      1435
                     /gene="Ndufv2"
                     /gene_synonym="2900010C23Rik"
ORIGIN      
        1 gtcctgggcg cggggccgcg cgcgccgggc ctccgggctc gttcgttccc agtcagccta
       61 gagcggccgg ggtcggagca tcgcgtaccc cgccatgttc tccttggcgc tgcgggccag
      121 ggcgaccggc ctcgctgctc agtggggaag acatgcaagg aatttgcata agacagcagt
      181 gcacaatggt gctggaggag ccttatttgt gcatagagat actcctgaga ataacccaga
      241 tactccattt gatttcacac cagaaaacta taagaggata gaggcaatag taaaaaacta
      301 cccagaaggg catcaagccg ctgctgtgct tccagtcctg gatctcgccc aaaggcagaa
      361 tggatggcta cctatctccg ctatgaacaa ggtggctgaa gttttacaag tacctccaat
      421 gagagtatat gaagtagcaa ctttttatac aatgtataat cgaaagccag ttgggaagta
      481 ccatatccag gtctgcacta ctacaccttg catgctgcga gattctgaca gcatattgga
      541 gacccttcag agaaagcttg gaataaaggt tggagagact acacctgaca aacttttcac
      601 tcttatagaa gtggaatgtt taggggcctg tgtaaatgca ccgatggttc aaataaatga
      661 caactactat gaggatctga cacccaagga tattgaagag attattgatg aactcaaagc
      721 tggaaaagtt cccaaaccag ggccaaggag tggccgcttc tgttgtgagc cagctggagg
      781 ccttacttct ttgactgaac cacccaaagg acctggcttt ggtgtgcaag caggccttta
      841 acttgtattc aactgtaaaa aatgtcactg gacaaataaa atatgaactt ccctacataa
      901 gctttattcg gtttgcttat tcccacctgt acatcctggc agtaatctag taacttgcag
      961 ctgctaaata ttatgtcaaa acaacaggac cactatctcg gtctacctgt gaagacctat
     1021 cattataaca gaatcaagtt aagaattatt ttagtgttgc ccattcatag agccatgtca
     1081 tttttatcag tgaagtaaaa gttttgcaaa acttaaaata catttttcct ttgtatcttt
     1141 agacacagcc aaattgttaa attttttata agctttatgt caatgttccc tttaagcgtt
     1201 ttaacattta agagcatata gttgttactg aagtttctga ctggtaagac ctccctatca
     1261 gtactaccgg taagagaatt ttctcaaagt attccattta tgtttgctat ctcattctgt
     1321 agccctgctt gtatgtttac caggctggcc ttcaactttg ggtagttttg cttctgcctc
     1381 tcaagttctg ggattgtggt aaaagcatta tttataaaaa gaaaagttaa aataatttga
     1441 tatttcttta gctatattta tttattttga tacaggatct cactttgtag atcagactgg
     1501 actagaactc agagatttag taaagatatt caaacccacc
//
