LOCUS       NM_001406498            1495 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor
            1 (Ndufaf1), transcript variant 3, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001406498 XM_006500149
VERSION     NM_001406498.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Wang,Y., Yasmin,L., Li,L., Gao,P., Xu,X., Sun,X. and Godbout,R.
  TITLE     DDX1 vesicles control calcium-dependent mitochondrial activity in
            mouse embryos
  JOURNAL   Nat Commun 13 (1), 3794 (2022)
   PUBMED   35778392
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Kurogi,S., Sekimoto,T., Funamoto,T., Ota,T., Nakamura,S., Nagai,T.,
            Nakahara,M., Yoshinobu,K., Araki,K., Araki,M. and Chosa,E.
  TITLE     Development of an efficient screening system to identify novel bone
            metabolism-related genes using the exchangeable gene trap
            mutagenesis mouse models
  JOURNAL   Sci Rep 7, 40692 (2017)
   PUBMED   28106071
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1495)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   4  (bases 1 to 1495)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   5  (bases 1 to 1495)
  AUTHORS   Janssen,R., Smeitink,J., Smeets,R. and van Den Heuvel,L.
  TITLE     CIA30 complex I assembly factor: a candidate for human complex I
            deficiency?
  JOURNAL   Hum Genet 110 (3), 264-270 (2002)
   PUBMED   11935339
REFERENCE   6  (bases 1 to 1495)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844536.13.
            
            On May 16, 2022 this sequence version replaced XM_006500149.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1886471.1,
                                           SRR13422598.464392.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               AL844536.13        124231-124459       c
            230-887             AL844536.13        121640-122297       c
            888-1073            AL844536.13        119912-120097       c
            1074-1148           AL844536.13        118006-118080       c
            1149-1495           AL844536.13        117084-117430       c
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.97 cM"
     gene            1..1495
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /note="NADH:ubiquinone oxidoreductase complex assembly
                     factor 1"
                     /db_xref="GeneID:69702"
                     /db_xref="MGI:MGI:1916952"
     exon            1..229
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /inference="alignment:Splign:2.1.0"
     exon            230..887
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /inference="alignment:Splign:2.1.0"
     CDS             312..1298
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /note="isoform 1 is encoded by transcript variant 3; NADH
                     dehydrogenase (ubiquinone) 1 assembly factor 1; complex I
                     intermediate-associated protein 30, mitochondrial; NADH
                     dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly
                     factor 1"
                     /codon_start=1
                     /product="complex I intermediate-associated protein 30,
                     mitochondrial isoform 1"
                     /protein_id="NP_001393427.1"
                     /db_xref="GeneID:69702"
                     /db_xref="MGI:MGI:1916952"
                     /translation="MSSIHKLLTGIYIHKNFLRPRAALPPFVGSHCVDYSSSSLQKKV
                     TSVDRASQQGKTEEGLQGHDHKEVALDAPSPDRTPEVSFDKAIRDEAIEHFRRLKDEI
                     VAHLRGPDGRPLQEVIMEQARVVWQFREKEDLDKWIVTSDKTIGGRSEIFLKMSKNNR
                     SALLYGTLSSEPPQDGDSRQSGYCAMISRIPRGAFERKLSYDWSQFNTLYLRVRGDGR
                     PWMVNIRQDTEFIQRKNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNQGRVRDVQGPLV
                     LDKISSIGFTLSDKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPVLNPRLFR"
     exon            888..1073
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1148
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1495
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1470..1475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /note="hexamer: AATAAA"
     polyA_site      1495
                     /gene="Ndufaf1"
                     /gene_synonym="2410001M24Rik; CGI-65; CIA30"
                     /note="major polyA site"
ORIGIN      
        1 agaaggacta ttcgggggaa gccgaatgtg gggtctgaga aggtgagcgg aaccttctca
       61 taggtgggaa ctgcagggac gaggaggtcg ggaagagtcc tggcgggtga ggtgagggtc
      121 gaggggctgc cactcctcgg gagcgcctag cttcaggagg ctgggacgtg accaaccccg
      181 cgcctgccga gaggtgaacg cctggtcttc agagtgagtt tggtctctgg tggaattggg
      241 cttattgttg aagcttgttt tctcagcccg ggcaaatttt tacattttaa tacttttttc
      301 ttacgatggc aatgtcttcc attcacaaat tactgactgg catttacatt cataaaaatt
      361 tcctgaggcc aagggctgca ttacctccat ttgtgggtag tcactgtgta gactattctt
      421 ccagtagtct tcagaaaaaa gtgacttctg ttgacagagc ctcccagcag ggaaagactg
      481 aagagggctt acaaggacat gaccacaaag aagttgcttt ggatgcacct tctcctgaca
      541 ggacacctga agttagtttt gataaagcaa ttagagatga agcaatagaa cattttagac
      601 gtttgaagga tgaaattgtg gctcatttga gaggcccaga cggccgccca ttacaagagg
      661 tcataatgga acaagccagg gttgtctggc agtttcgtga aaaagaagat ttggataagt
      721 ggatagtgac ttctgataag acaattggag gtagaagtga aatattcttg aaaatgagca
      781 agaacaaccg aagtgccctg ctttatggaa ctctgagctc tgagcctcct caggatgggg
      841 acagtagaca aagtggatac tgtgccatga tctccaggat tccacggggt gcttttgaga
      901 ggaagctgtc ttatgattgg tcccagttca atactctgta tctccgagtt cgtggggatg
      961 gacggccttg gatggtaaat atcaggcaag acacggagtt tatccagaga aaaaatcaga
     1021 tgtacagtta cttcatgttc acccgtgggg gaccctactg gcaggaagtc aagattcctt
     1081 tctccaaatt tttcttttca aatcaaggaa gggtacgaga tgtccaaggc ccactcgtat
     1141 tggacaagat ctcttctata ggattcactc tgtctgataa ggtggatgga ccattcttcc
     1201 tggaaataga tttcattggt gtgtttactg atccagccca tacagaagaa tttgcttatg
     1261 agaattctcc cgttcttaac ccaagactct tcagatagag aactggggac gtcagtagag
     1321 tagtggtcac aaagcagaca gagcctttct gtctacccga cccctttcgt agtacttact
     1381 taagacggta agtacagcat ggcctctcag tggaagttct tctgagtgct tacataaaga
     1441 tgaatcaaag gaagtgtact gaaaacaaaa ataaagcctt tctgcctacc accta
//
