LOCUS       NM_023202                540 bp    mRNA    linear   ROD 18-NOV-2024
DEFINITION  Mus musculus NADH:ubiquinone oxidoreductase subunit A7 (Ndufa7),
            transcript variant 1, mRNA.
ACCESSION   NM_023202
VERSION     NM_023202.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Vercellino,I. and Sazanov,L.A.
  TITLE     SCAF1 drives the compositional diversity of mammalian respirasomes
  JOURNAL   Nat Struct Mol Biol 31 (7), 1061-1071 (2024)
   PUBMED   38575788
REFERENCE   2  (bases 1 to 540)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 540)
  AUTHORS   Shi,X., Zhang,Y., Chen,R., Gong,Y., Zhang,M., Guan,R.,
            Rotstein,O.D., Liu,X. and Wen,X.Y.
  TITLE     ndufa7 plays a critical role in cardiac hypertrophy
  JOURNAL   J Cell Mol Med 24 (22), 13151-13162 (2020)
   PUBMED   32989924
  REMARK    GeneRIF: ndufa7 plays a critical role in cardiac hypertrophy.
REFERENCE   4  (bases 1 to 540)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 540)
  AUTHORS   Logunova,N., Korotetskaya,M., Polshakov,V. and Apt,A.
  TITLE     The QTL within the H2 Complex Involved in the Control of
            Tuberculosis Infection in Mice Is the Classical Class II H2-Ab1
            Gene
  JOURNAL   PLoS Genet 11 (11), e1005672 (2015)
   PUBMED   26618355
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 540)
  AUTHORS   Hoefs,S.J., Rodenburg,R.J., Smeitink,J.A. and van den Heuvel,L.P.
  TITLE     Molecular base of biochemical complex I deficiency
  JOURNAL   Mitochondrion 12 (5), 520-532 (2012)
   PUBMED   22820119
  REMARK    GeneRIF: The authors provide an overview of the function and
            assembly of human complex I, also known as NADH dehydrogenase
            (ubiquinone) 1 alpha subcomplex. They discuss its role in oxidative
            phosphorylation and the implications of complex I deficiency in
            disease.
            Review article
REFERENCE   7  (bases 1 to 540)
  AUTHORS   Wirtz,S. and Schuelke,M.
  TITLE     Region-specific expression of mitochondrial complex I genes during
            murine brain development
  JOURNAL   PLoS One 6 (4), e18897 (2011)
   PUBMED   21556144
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 540)
  AUTHORS   Abe,K., Yuzuriha,M., Sugimoto,M., Ko,M.S., Brathwaite,M., Waeltz,P.
            and Nagaraja,R.
  TITLE     Gene content of the 750-kb critical region for mouse embryonic
            ectoderm lethal tcl-w5
  JOURNAL   Mamm Genome 15 (4), 265-276 (2004)
   PUBMED   15112104
REFERENCE   9  (bases 1 to 540)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Proteomic analysis of the mammalian mitochondrial ribosome.
            Identification of protein components in the 28 S small subunit
  JOURNAL   J Biol Chem 276 (35), 33181-33195 (2001)
   PUBMED   11402041
REFERENCE   10 (bases 1 to 540)
  AUTHORS   Sazanov,L.A., Peak-Chew,S.Y., Fearnley,I.M. and Walker,J.E.
  TITLE     Resolution of the membrane domain of bovine complex I into
            subcomplexes: implications for the structural organization of the
            enzyme
  JOURNAL   Biochemistry 39 (24), 7229-7235 (2000)
   PUBMED   10852722
  REMARK    GeneRIF: The authors characterize complex I, also known as NADH
            dehydrogenase (ubiquinone) 1 alpha subcomplex,  that was purified
            from bovine heart mitochondria. Biochemical treatment with
            detergent dissociated the complex into four subfractions. Ndufa7,
            also known as B14.5a, was found in the I-lambda fraction.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT030732.13, BC055698.1 and
            BX522503.1.
            
            On Jun 4, 2013 this sequence version replaced NM_023202.3.
            
            Summary: This gene encodes a subunit of the NADH-ubiquinone
            oxidoreductase (complex I) enzyme, which is a large, multimeric
            protein. It is the first enzyme complex in the mitochondrial
            electron transport chain and catalyzes the transfer of electrons
            from NADH to the electron acceptor ubiquinone. The proton gradient
            created by electron transfer drives the conversion of ADP to ATP.
            Complex I has been biochemically separated into four fractions. The
            bovine ortholog of this protein has been reported to be part of the
            I-lambda fraction, which forms the extrinsic globular domain. In
            humans, deficiencies in complex I are associated with myopathies,
            encephalomyopathies, and neurodegenerative disorders. Pseudogenes
            of this gene are located on chromosomes 7 and 16. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, May 2013].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CF738649.1, BU937539.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 10852722; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                CT030732.13        135907-135941
            36-520              BC055698.1         1-485
            521-540             BX522503.1         249-268
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 17.98 cM"
     gene            1..540
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /note="NADH:ubiquinone oxidoreductase subunit A7"
                     /db_xref="GeneID:66416"
                     /db_xref="MGI:MGI:1913666"
     exon            1..109
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..400
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /note="complex I-B14.5a; NADH-ubiquinone oxidoreductase
                     subunit B14.5a; NADH dehydrogenase (ubiquinone) 1 alpha
                     subcomplex, 7 (B14.5a)"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 alpha
                     subcomplex subunit 7"
                     /protein_id="NP_075691.1"
                     /db_xref="CCDS:CCDS28631.1"
                     /db_xref="GeneID:66416"
                     /db_xref="MGI:MGI:1913666"
                     /translation="MASATRVIQKLRNWASGQDLQAKLQLRYQEIAKRTQPPPKLPVG
                     PSHKLSNNYYCTRDGRREVVPPSIIMSSQKALVSGKAAESSAMAATEKKAVTPAPPMK
                     RWELSKDQPYL"
     exon            110..159
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /inference="alignment:Splign:2.1.0"
     exon            160..309
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /inference="alignment:Splign:2.1.0"
     exon            310..527
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      500..505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /note="hexamer: AATATA"
     polyA_site      520
                     /gene="Ndufa7"
                     /gene_synonym="14.5kDa; 2400007M02Rik; CI-B14.5a"
                     /note="major polyA site"
ORIGIN      
        1 ggtacccagg aatcctcggg acagagtcgt caccagcgtc cttcggagcg gaaggaatat
       61 ggcgtccgct actcgcgtta tccaaaagct gcggaactgg gcgtctgggc aagacctgca
      121 ggcgaagcta cagctgcgct accaggagat cgccaagcgg acccagccac ctccgaaact
      181 ccccgtgggc cccagtcaca agctgtccaa caattactac tgtactcgtg atggccgccg
      241 ggaagttgtg cctccctcaa tcatcatgtc ctcacaaaag gccctggtgt caggcaaggc
      301 cgccgagagt tctgcaatgg cagccactga gaagaaggca gtgacacctg ctcctcccat
      361 gaagaggtgg gagctgtcca aggaccagcc atacctgtga ccctgcccta ggttaccttg
      421 ctatatatgt ctctagggcc acatgactgc ttttcctcct tggactccct ctggggagag
      481 tgtgacctaa tttgtaacaa atatatagaa ttccacatta tttccaaaaa aaaaaaaaaa
//
