LOCUS       NM_010885                602 bp    mRNA    linear   ROD 18-NOV-2024
DEFINITION  Mus musculus NADH:ubiquinone oxidoreductase subunit A2 (Ndufa2),
            mRNA.
ACCESSION   NM_010885
VERSION     NM_010885.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Wu,Y., Chen,S., Huang,G., Zhang,L., Zhong,L., Feng,Y., Wen,P. and
            Liu,J.
  TITLE     Transcriptome analysis reveals EBF1 ablation-induced injuries in
            cardiac system
  JOURNAL   Theranostics 14 (12), 4894-4915 (2024)
   PUBMED   39239522
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  AUTHORS   Vercellino,I. and Sazanov,L.A.
  TITLE     SCAF1 drives the compositional diversity of mammalian respirasomes
  JOURNAL   Nat Struct Mol Biol 31 (7), 1061-1071 (2024)
   PUBMED   38575788
REFERENCE   3  (bases 1 to 602)
  AUTHORS   Sharma,S., Kajjo,S., Harra,Z., Hasaj,B., Delisle,V., Ray,D.,
            Gutierrez,R.L., Carrier,I., Kleinman,C., Morris,Q., Hughes,T.R.,
            McInnes,R. and Fabian,M.R.
  TITLE     Uncovering a mammalian neural-specific poly(A) binding protein with
            unique properties
  JOURNAL   Genes Dev 37 (15-16), 760-777 (2023)
   PUBMED   37704377
REFERENCE   4  (bases 1 to 602)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 602)
  AUTHORS   Dieteren,C.E., Koopman,W.J., Swarts,H.G., Peters,J.G., Maczuga,P.,
            van Gemst,J.J., Masereeuw,R., Smeitink,J.A., Nijtmans,L.G. and
            Willems,P.H.
  TITLE     Subunit-specific incorporation efficiency and kinetics in
            mitochondrial complex I homeostasis
  JOURNAL   J Biol Chem 287 (50), 41851-41860 (2012)
   PUBMED   23038253
  REMARK    GeneRIF: The authors use green fluorescent-tagged complex I
            subunits to determine the kinetics of assembly. Tagged human NDUFA2
            may incorporate into the holo-complex I enzyme as an assembly
            intermediate or as free monomers. The authors conclude that
            nuclear-encoded subunits incorporate into the holo-enzyme in a
            subunit-specific manner.
REFERENCE   6  (bases 1 to 602)
  AUTHORS   Kim,S.C., Sprung,R., Chen,Y., Xu,Y., Ball,H., Pei,J., Cheng,T.,
            Kho,Y., Xiao,H., Xiao,L., Grishin,N.V., White,M., Yang,X.J. and
            Zhao,Y.
  TITLE     Substrate and functional diversity of lysine acetylation revealed
            by a proteomics survey
  JOURNAL   Mol Cell 23 (4), 607-618 (2006)
   PUBMED   16916647
REFERENCE   7  (bases 1 to 602)
  AUTHORS   Youngren,K.K., Coveney,D., Peng,X., Bhattacharya,C., Schmidt,L.S.,
            Nickerson,M.L., Lamb,B.T., Deng,J.M., Behringer,R.R., Capel,B.,
            Rubin,E.M., Nadeau,J.H. and Matin,A.
  TITLE     The Ter mutation in the dead end gene causes germ cell loss and
            testicular germ cell tumours
  JOURNAL   Nature 435 (7040), 360-364 (2005)
   PUBMED   15902260
REFERENCE   8  (bases 1 to 602)
  AUTHORS   Brockmann,C., Diehl,A., Rehbein,K., Strauss,H., Schmieder,P.,
            Korn,B., Kuhne,R. and Oschkinat,H.
  TITLE     The oxidized subunit B8 from human complex I adopts a thioredoxin
            fold
  JOURNAL   Structure 12 (9), 1645-1654 (2004)
   PUBMED   15341729
  REMARK    GeneRIF: The authors determine the NMR structure of the oxidized
            human NDUFA2 protein, also known as B8, and report that its
            structure and redox potential is similar to that of thioredoxins.
            The highly conserved residues on the surface most likely form the
            protein-protein interaction site of this subunit with the large
            multimeric complex I.
REFERENCE   9  (bases 1 to 602)
  AUTHORS   Harrison,M. and Singh,S.M.
  TITLE     Genetics and differential expression of NADH:ubiquinone
            oxidoreductase B8 subunit in brains of genetic strains of mice
            differing in voluntary alcohol consumption
  JOURNAL   Biochim Biophys Acta 1579 (2-3), 164-172 (2002)
   PUBMED   12427551
  REMARK    GeneRIF: differentially expressed in the brains of four mice
            strains; expression pattern follows ethanol preference of the
            strains [(NADH-ubiquinone oxidoreductase B8 subunit]
REFERENCE   10 (bases 1 to 602)
  AUTHORS   Murdock,D.G., Boone,B.E., Esposito,L.A. and Wallace,D.C.
  TITLE     Up-regulation of nuclear and mitochondrial genes in the skeletal
            muscle of mice lacking the heart/muscle isoform of the adenine
            nucleotide translocator
  JOURNAL   J Biol Chem 274 (20), 14429-14433 (1999)
   PUBMED   10318868
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AV482131.1, AK003608.1 and
            AC027740.11.
            
            On May 30, 2013 this sequence version replaced NM_010885.4.
            
            Summary: This gene encodes a subunit of the NADH-ubiquinone
            oxidoreductase (complex I) enzyme, which is a large, multimeric
            protein. It is the first enzyme complex in the mitochondrial
            electron transport chain and catalyzes the transfer of electrons
            from NADH to the electron acceptor ubiquinone. The proton gradient
            created by electron transfer drives the conversion of ADP to ATP.
            The human ortholog of this gene has been characterized, and its
            structure and redox potential is reported to be similar to that of
            thioredoxins. It may be involved in regulating complex I activity
            or assembly via assistance in redox processes. In humans, mutations
            in this gene are associated with Leigh syndrome, an early-onset
            progressive neurodegenerative disorder. A pseudogene of this gene
            is located on chromosome 5. [provided by RefSeq, May 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BQ746689.1, AV482131.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 23038253; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AV482131.1         1-39
            40-566              AK003608.1         2-528
            567-602             AC027740.11        198879-198914       c
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 19.46 cM"
     gene            1..602
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /note="NADH:ubiquinone oxidoreductase subunit A2"
                     /db_xref="GeneID:17991"
                     /db_xref="MGI:MGI:1343103"
     exon            1..168
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..367
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /EC_number="7.1.1.2"
                     /note="NADH-ubiquinone oxidoreductase B8 subunit; complex
                     I-B8; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,
                     2"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 alpha
                     subcomplex subunit 2"
                     /protein_id="NP_035015.2"
                     /db_xref="CCDS:CCDS29161.1"
                     /db_xref="GeneID:17991"
                     /db_xref="MGI:MGI:1343103"
                     /translation="MAAAAASRAVGAKLGLREIRVHLCQRSPGSQGVRDFIVQRYVEL
                     KKAHPNLPILIRECSEVQPKLWARYAFGQEKTVSLNNLSADEVTRAMQNVLSGKA"
     exon            169..275
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /inference="alignment:Splign:2.1.0"
     exon            276..602
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      451..456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /note="hexamer: TATAAA"
     polyA_site      473
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
                     /note="major polyA site"
     polyA_site      597
                     /gene="Ndufa2"
                     /gene_synonym="B8; C1-B8; CI-B8"
ORIGIN      
        1 tggagggccc gctattggtc cgattgaggg tgaaaggtcg tgattgggcg ctcctttgct
       61 aacaaagatg gcggctgccg ctgctagccg agcggtcggc gcaaagctgg ggttgcgtga
      121 gattcgcgtt cacttatgcc agcgttcccc aggcagccag ggtgtgaggg atttcatcgt
      181 gcaacggtac gtggagctga agaaggcgca ccccaacctg cccattctga tccgcgaatg
      241 ctcggaggtg cagcccaagc tttgggcccg ctatgctttt ggccaagaga agacggtgtc
      301 tctgaacaat ctgagtgctg atgaggtaac cagagccatg cagaatgtgc taagcggcaa
      361 agcctgaagg tctccactga ggactgtgag cgagagcagc tgaacctgct ggactgaaga
      421 cagtgtgggg aaatgtgtgc tttgggtcct tataaagctt acgctgtaca gtgtcccttc
      481 agaatgtcct cttcattacc ttctccctct tactgcgcaa cactgaggca aagtagtttt
      541 atataaaaat actcctttat ttctcctcaa aaaaaaaaaa aaaaaaaacc caccaggtgc
      601 ca
//
