LOCUS       NM_022565               7400 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus N-deacetylase/N-sulfotransferase (heparin
            glucosaminyl) 4 (Ndst4), mRNA.
ACCESSION   NM_022565 XM_913975
VERSION     NM_022565.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Wu,D., Schieren,I., Qian,Y., Zhang,C., Jessell,T.M. and de
            Nooij,J.C.
  TITLE     A Role for Sensory end Organ-Derived Signals in Regulating Muscle
            Spindle Proprioceptor Phenotype
  JOURNAL   J Neurosci 39 (22), 4252-4267 (2019)
   PUBMED   30926747
REFERENCE   3  (bases 1 to 7400)
  AUTHORS   Jao,T.M., Li,Y.L., Lin,S.W., Tzeng,S.T., Yu,I.S., Yen,S.J.,
            Tsai,M.H. and Yang,Y.C.
  TITLE     Alteration of colonic epithelial cell differentiation in mice
            deficient for glucosaminyl N-deacetylase/N-sulfotransferase 4
  JOURNAL   Oncotarget 7 (51), 84938-84950 (2016)
   PUBMED   27793051
  REMARK    GeneRIF: this study revealed the involvement of Ndst4 in the
            development and homeostasis of colonic epithelium
REFERENCE   4  (bases 1 to 7400)
  AUTHORS   Conrad,E., Dai,C., Spaeth,J., Guo,M., Cyphert,H.A., Scoville,D.,
            Carroll,J., Yu,W.M., Goodrich,L.V., Harlan,D.M., Grove,K.L.,
            Roberts,C.T. Jr., Powers,A.C., Gu,G. and Stein,R.
  TITLE     The MAFB transcription factor impacts islet alpha-cell function in
            rodents and represents a unique signature of primate islet
            beta-cells
  JOURNAL   Am J Physiol Endocrinol Metab 310 (1), E91-E102 (2016)
   PUBMED   26554594
REFERENCE   5  (bases 1 to 7400)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   6  (bases 1 to 7400)
  AUTHORS   Pan,Y., Woodbury,A., Esko,J.D., Grobe,K. and Zhang,X.
  TITLE     Heparan sulfate biosynthetic gene Ndst1 is required for FGF
            signaling in early lens development
  JOURNAL   Development 133 (24), 4933-4944 (2006)
   PUBMED   17107998
REFERENCE   7  (bases 1 to 7400)
  AUTHORS   Yabe,T., Hata,T., He,J. and Maeda,N.
  TITLE     Developmental and regional expression of heparan sulfate
            sulfotransferase genes in the mouse brain
  JOURNAL   Glycobiology 15 (10), 982-993 (2005)
   PUBMED   15944372
REFERENCE   8  (bases 1 to 7400)
  AUTHORS   Ford-Perriss,M., Guimond,S.E., Greferath,U., Kita,M., Grobe,K.,
            Habuchi,H., Kimata,K., Esko,J.D., Murphy,M. and Turnbull,J.E.
  TITLE     Variant heparan sulfates synthesized in developing mouse brain
            differentially regulate FGF signaling
  JOURNAL   Glycobiology 12 (11), 721-727 (2002)
   PUBMED   12460940
REFERENCE   9  (bases 1 to 7400)
  AUTHORS   Grobe,K. and Esko,J.D.
  TITLE     Regulated translation of heparan sulfate N-acetylglucosamine
            N-deacetylase/n-sulfotransferase isozymes by structured
            5'-untranslated regions and internal ribosome entry sites
  JOURNAL   J Biol Chem 277 (34), 30699-30706 (2002)
   PUBMED   12070138
REFERENCE   10 (bases 1 to 7400)
  AUTHORS   Aikawa,J., Grobe,K., Tsujimoto,M. and Esko,J.D.
  TITLE     Multiple isozymes of heparan sulfate/heparin GlcNAc
            N-deacetylase/GlcN N-sulfotransferase. Structure and activity of
            the fourth member, NDST4
  JOURNAL   J Biol Chem 276 (8), 5876-5882 (2001)
   PUBMED   11087757
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140797.10, AC164153.2 and AC111141.7.
            
            On Oct 6, 2022 this sequence version replaced NM_022565.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR6116558.5515.1,
                                           SRR9219381.280650.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164136
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-413               AC140797.10        106651-107063
            414-1628            AC140797.10        140103-141317
            1629-1716           AC164153.2         115442-115529
            1717-1871           AC164153.2         124761-124915
            1872-2057           AC164153.2         163486-163671
            2058-2186           AC164153.2         165524-165652
            2187-2369           AC111141.7         133477-133659       c
            2370-2466           AC111141.7         118798-118894       c
            2467-2590           AC111141.7         108594-108717       c
            2591-2765           AC111141.7         106576-106750       c
            2766-2936           AC111141.7         103325-103495       c
            2937-3046           AC111141.7         102140-102249       c
            3047-3149           AC111141.7         94597-94699         c
            3150-7400           AC111141.7         87898-92148         c
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 54.68 cM"
     gene            1..7400
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="N-deacetylase/N-sulfotransferase (heparin
                     glucosaminyl) 4"
                     /db_xref="GeneID:64580"
                     /db_xref="MGI:MGI:1932545"
     exon            1..413
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            414..1628
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             651..3269
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /EC_number="2.8.2.8"
                     /note="N-deacetylase/N-sulfotransferase 4; NDST-4; N-HSST
                     4; N-heparan sulfate sulfotransferase 4; glucosaminyl
                     N-deacetylase/N-sulfotransferase 4"
                     /codon_start=1
                     /product="bifunctional heparan sulfate
                     N-deacetylase/N-sulfotransferase 4"
                     /protein_id="NP_072087.2"
                     /db_xref="CCDS:CCDS17820.1"
                     /db_xref="GeneID:64580"
                     /db_xref="MGI:MGI:1932545"
                     /translation="MNLILKFRRSFRTLIVLLATFCLVSILISAYFLYSGYKQEMTLI
                     ETTAEAECADIKDLPYRSIELRTIKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESS
                     RFQYQMVIAPGKGDIPPLTDSGKGKYTLIIYENILKYVSMDSWNRELLEKYCIEYSVS
                     IIGFHKANENSLPTTQLKGFPLNLFNNVALKDCSVNPQSPLLHITKGPKVEKGPLPGE
                     DWTIFQYNHSTYQPVLLTELQSEKSLSFLSSQTLYATIIQDLGLHDGIQRVLFGNNLN
                     FWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNL
                     LRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNE
                     SSLVEQMILNKEFALEHGIPINLGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEE
                     YPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIKGGELFLTI
                     LLNPISIFMTHLSNYGNDRLGLYTFVNLANFVHSWTNLKLQTLPPVQLAHKYFELFPE
                     QKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNL
                     PSPKTFEEVQFFNGNNYHKGIEWYMDFFPTPSNITSDFLFEKSANYFHSEEAPKRAAS
                     LVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVITTGHWAPPDLKTLQRR
                     CLVPGWYAVHIERWLAYFSTSQLLIIDGQQLRSDPATVMDEVQKFLGVTPHYNYSEAL
                     TFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDSESRTFLSSYYRDHNVELSKLLHRL
                     GQPLPSWLRQELQKVR"
     exon            1629..1716
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1871
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2057
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2186
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2369
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2466
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2590
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2765
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2936
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2937..3046
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3047..3149
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..7400
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3464..3469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3487
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="major polyA site"
     regulatory      6818..6823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="hexamer: AATAAA"
     regulatory      6832..6837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      6840
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="major polyA site"
     polyA_site      6867
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
     regulatory      7155..7160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7194
                     /gene="Ndst4"
                     /gene_synonym="4930439H17Rik"
ORIGIN      
        1 aagcaagagg cacagcagca gtggcagaag cagacaggag cagctggtat tccggtgatt
       61 aaatagttct catagccttt ttgctgtacc cggtctgctc cctggggtaa ggctcgtgtc
      121 cgagaaggaa gctgcatttt aacggtgtgc tgaacagagg tctgaatcat tccagctggc
      181 tgcttgtttt gaaaaggaca atgtgtgtat aaataaaaag actgctggaa gggtccagac
      241 accggcaccg gagctgagga gtgctctcag aatcctgttg aggcagatgg cacgaactga
      301 aagctccgct aattaacgtg gagccaagta aacctgaatt ctggatatct cattttctaa
      361 cttcggataa attcaagtta gcaaaagaaa aaaaaattga aatgcttctc tagataatgc
      421 ttaaaggacc ctgcagcagt tcttggtatt ggacatagca tctcagctgt tgcctcctgg
      481 tccgctgact accagatttg actcatttgt gattccttcg agaatctccc atgatttgca
      541 atggttacat gaatgtttgg gaaggaaaac tggcaacatc ttagtatatg ttctccatgg
      601 cacattaggg aaagaagcca gaaaaggctc ccaacgcatt gatttttaaa atgaatctta
      661 ttttaaagtt tcgaagaagc tttcgaacac tgattgttct cttagctact ttttgcttgg
      721 tgagcatcct catttctgcc tacttcctgt actctggcta caaacaggaa atgacactta
      781 ttgaaacgac tgcagaagca gagtgtgctg acatcaaaga cctcccttat cggtctatag
      841 agctgagaac catcaagcct attgatacat ccaaaactga ccccactgtc cttctctttg
      901 tagagagcca gtattcgcaa cttggtcaag atattatagc aatcttggag tccagtcggt
      961 ttcaatacca gatggtcatt gctccaggca agggagacat accgcctctt accgatagtg
     1021 gcaaagggaa gtacacattg attatttatg aaaatattct gaagtacgtc agcatggact
     1081 catggaaccg ggagctttta gaaaaatact gtatagaata cagtgttagc ataattggtt
     1141 ttcataaagc caatgagaac agtttaccaa ctacacaact gaaagggttt cctttgaact
     1201 tattcaataa cgtagctctg aaggactgct ctgtgaaccc ccagtctcct ctgctccata
     1261 ttaccaaagg ccccaaagtt gaaaagggac ctcttcctgg ggaagactgg acaattttcc
     1321 agtataacca ttctacctat cagccagtgc ttttaactga gttacagtca gaaaagtccc
     1381 tctctttctt gtccagccaa acactttatg cgacaattat tcaggatctg gggcttcatg
     1441 acgggattca gcgagttctt tttggtaaca acttgaactt ttggttgcac aagctcattt
     1501 tcatagacgc catctccttc ttgtcgggga agaggttgac actgtccttg gacaggtaca
     1561 tccttgtgga cattgatgac atatttgtgg ggaaggaggg cacgagaatg aatgtcaaag
     1621 atgtgaaggc attactagag actcaaaatt tactgcgcac tcaggttgca aattttacct
     1681 tcaaccttgg attttcaggg aagttttacc acacaggaac cgaagaagag gatgaaggtg
     1741 acgaccttct gctgaggtct gtggatgaat tctggtggtt tcctcacatg tggagtcaca
     1801 tgcagcccca cctcttccac aatgaatcat ctttagtgga acagatgatt ctcaacaaag
     1861 aatttgccct ggaacatgga attccgatca acctgggcta cgcagtggcc ccacatcact
     1921 caggggtcta cccagtccat attcagttgt atgcagcttg gaagaaagtc tggggtattc
     1981 aggtcaccag cactgaagag tacccacatc tgaaacctgc gcggtacagg aagggcttca
     2041 ttcataatag catcatggtc ctccctcgac agacctgtgg gctgttcaca catactattt
     2101 tctacaagga gtatccagga ggacctcaag aattggataa aagtatcaaa ggaggtgaac
     2161 tcttcctcac aatccttcta aatccaatca gcattttcat gacacatctt tctaactatg
     2221 gaaatgaccg cctcggcttg tacacctttg tgaacttggc caactttgtg cacagctgga
     2281 ccaacttgaa actgcagacc ctgcctccag tgcaactggc ccacaaatac ttcgagctct
     2341 tccctgagca gaaagaccct ctctggcaga atccatgcga cgataaacga cacaaagaca
     2401 tctggtccag ggagaaaacc tgtgaccatt taccgaaatt ccttgtgatt gggccacaga
     2461 agacaggaac aactgcactt tatttatttc ttctaatgca cccctccatc atcagcaatc
     2521 ttcctagccc caaaaccttt gaagaagttc aattttttaa tggcaacaac tatcacaagg
     2581 gaattgagtg gtatatggac tttttcccca ctccttccaa cattaccagt gatttcctat
     2641 ttgaaaagag tgctaactac ttccactcag aagaggctcc caagagggct gcatctcttg
     2701 ttcccaaagc caagatcatt actatcctca tagacccctc ggacagggca tattcttggt
     2761 accagcacca acgatctcat gaagatccag ctgccctgag gttcaatttc tatgaagtta
     2821 tcacaacagg acattgggcc cctcctgatt taaaaacatt gcagaggaga tgtctggtgc
     2881 ctgggtggta tgcagtgcac atagaaagat ggctggccta cttttctact tcccagttgc
     2941 taattattga tggacaacag ctgagatctg acccagctac tgtgatggat gaagtccaga
     3001 agtttctagg agttacacct cattataatt actcggaagc actaacgttt gatccccaga
     3061 aaggcttttg gtgtcagcta ctagaaggag gaaaaaccaa atgccttggg aaaagcaaag
     3121 gccgaaaata cccgcccatg gattcagagt ccagaacttt cctctccagc tactaccgcg
     3181 accacaacgt ggaactctcg aagctgctgc acaggctggg gcagcctctg ccctcgtggc
     3241 tgagacagga gctgcagaaa gtgaggtagc actcgtgagg agaacatggc accgagtcac
     3301 cattaagtag aaaaggcaaa ccaagcaact ctatcctttt catacgcgtg tgattatcag
     3361 gagaggactg tgaataaatt cctatcgatg ttttctgtca aatgaaaaca tatatgcacg
     3421 cattggcaat tattagcatt attctttact taaggctttt ggaaataaaa tatggggttt
     3481 gcggcaatat cttgtgtcta gtctggggat caccatacag ctcacttcct ctgtcatcca
     3541 gatttcaggt caccacttca ctaatgctct gactgtatgc aaattaccaa cttaaactat
     3601 gaggaaaaga taagagtcta aaaatgaatt caggtttaac ccagcgtctg aatgaagggc
     3661 taatgtttct aagcaccatt tatccattgg tccagcatcc cacttcatcg ctatggcatg
     3721 ccatcctcag aaaactccaa catggtctct ggagagcccc aggaaattgg gaaattgtga
     3781 ttctgaactg tagtacccta gaaagtgagg gccatcttta gtgggctcat ggtcttgcta
     3841 cattttcagt ctagattctc ctaccttggc agtgattgag catttataca tattttgttc
     3901 actattccaa atagggagaa gaaaaaactt tattgactag aaatgtaaac atctagatgt
     3961 atttgcagat ctaaacaggc attgagcctt ctctgtaaga catatgtcca tctatttgca
     4021 agcatgatat tttatatatg ccactacatc tactatgaca aagttttact gtctgtgttt
     4081 aaaaccattt aaaacttaca ttatactgca aacttaccaa tttgagcatc actaattctc
     4141 ttacttgaac tcaatttcat agacataaaa ctcagaaatt aattttaaaa gatgttttat
     4201 tttgttttga gttacaagtt cctatagtat ttgaagtcta tgtaagccac attaactgga
     4261 attttatcct ctttgtcaac aatgatctag aaaagcttaa tttatctttt gagtgataca
     4321 tgaaggtaaa tttactataa cagtcaacca aggtgacttt gggaacatta cccacatatc
     4381 tccctcctcc tgagtaattt ttctagaggc tgtaaataat aacatcattt attctctctt
     4441 taattcaaaa gacacatgta gtcttgtgtg tgtgtgtgtg tgtgtgtgtg tgagagagag
     4501 agagagagag agagagagag agagagagag agatgaatta gtcaaacaaa ttcaatatgg
     4561 ctattctaaa atagcattgg tgtctcttta cacattgaaa atcaacttat catctatatt
     4621 tcactactgt ttctgcttat tttctatttt tacttagcat ttctgtcgac ctttatcaga
     4681 aacatgtgag attccttttc ttctttattg gtataaatca caatattttc cagtttcgtt
     4741 tttagctgtg gggcttaacg tctcaccctt tttacttaca taaaagtgct atacatttta
     4801 ttaaaatggt gaaaaataac catgatgatt tatttagacc actactattt attcaccatt
     4861 cccctcctag ttctgttata taactagcgc agcctgcctt caagtgtgca atcctcctgc
     4921 cctcccctct gctgcactgg gctcacagtg ggcactgcta tgtctacttc taattacttt
     4981 attaacctaa ggaaataatc atctttcctt tataggagat tacagtgata gttatagagt
     5041 gaggatattt tcatatcaaa taatatcgat ataacgcaag aacatccaat gcatcaccta
     5101 atttagtaat gacatgaatt ttctgcattt tcacatttta ataatgtctc catatcagtg
     5161 acctacctaa caatatctga aagcaaactg tcaacccaac ttaactattc tctttttttt
     5221 cctggttatt cgaatcatta cacatatttt cttactatct tttataataa aatgagcaac
     5281 tatatttaat tattaacaag attttatcta aaagtttgaa ggaatctaga ctctgagatt
     5341 ctgcactgta gtaattggga aaactcacct ttaatatttt cctttttaaa gatttcttcc
     5401 cacttcagga atgttgtctc agaacctaat agtgtaattc tttagaattt taaattacat
     5461 cctcagtttt aagcgataca cattaaatat tagaattttt ttaaaatatc ctttagcaca
     5521 ctggcatggt tgtttcattc ttttccagac ttttggagcc ttgcaggact tacaaatata
     5581 cctgtcaact actttcactt tgttaatggt aaaaatataa tttaattgtt tctgatacta
     5641 aattataaaa gttatactat gaaaattcat ctaaaataaa tttagattct atttaacaat
     5701 aaccaaacat aattttaaaa aacatgcaga acagcagtac tagtttcaca cacacacaca
     5761 cacacacaca cacacacaca cacacacaca cacacaccaa aatgctctcc cctgccacac
     5821 acagaggtct aatagtttct ctatatctaa attttaacaa acccttctta catttaatct
     5881 tgctctgctt tttaagtatt actgctttac tgtaatacaa agtaacctat attctggact
     5941 tactgattga tctcttttaa attacaggaa acaaggtatt tatatagaac caatactaaa
     6001 gccttctatt tctgatggtg acagaaaatg ctttttgttt gagaaagggg gagggactca
     6061 aaaataacat gggccagctg acttcataaa gcagactgtc tagcctagta gtctattcat
     6121 attgatattt ttttcataat caactttaga aatgcaatcc tggcagaaaa ctgtacatac
     6181 acttcaaaaa tgtgcaaata tataaaaatg tgttgtgtct gagaaaccta aagacctttt
     6241 gatcacatca gtaattttct ttgtaaacca gtgagatacc ccagataggc agagagaaaa
     6301 ccctgtattg actgaaatga actgttaaaa aagctgttgt tgcccaagca taagggacca
     6361 tgaccatctc ttcctccaaa taaaccctgc tgctggtaca gccactggag tgatactgtt
     6421 tgtatgaaag ctggccttta agtgcgggtt gataactccc ttgtcttcat gattgcatac
     6481 acaatccctg gatgccaagc atcaaatgta aacccgggaa tgggttttca aagtcggaac
     6541 cctcatccca gttctgcctc tcagtagcct tgtggcctta gataatttgt ggaacttccc
     6601 tgagccacag tcttatttgt ttttttctgt acaataaaga caataaaaaa ttacccatga
     6661 ggttattata acctattact gaaaggatgt ttgcataaca gcacaatggc tgaaatagtt
     6721 gtgcttaaat atattagtca taaaaaataa tgtataacta atcatggctc tcttctcttc
     6781 catcaaacag gaatgtgtgt tttctactcc tgttaataat aaaatgtgtt taataaaaca
     6841 tgtaattgta tttggtccaa tgagaaatga tcttcctaat cattatccca aagaggagtc
     6901 tgggctgctc actgctctgc tctggtgatc agcatccaga gtgaggtagc gcctccttcg
     6961 ctcagacctc ctctcctcct cccaactttg tatttattca gtgactagtt ctgttctttc
     7021 tgtctatccc tgtaattctt ttctagccca gtggatacat tgccaaaaat gtcagatgga
     7081 gaccgctggt tttttcaaca aatgagaggt ttaatggtgg tagatgtgag aatatattct
     7141 acgaaggcat gtataataaa gaattaattc tttcaatctg tatataaaaa agtacaagaa
     7201 aatgtctttg agtctctaca aatctcaact tcaaccatct aatgaaagct tttgttctgt
     7261 agatgaaaac actttattat atacatttat attatatatt tataaatatc aaacattatt
     7321 ttctgtattg tcccatgaca aatatataat acatccaaga taaatcagca gacaaacaat
     7381 taaaaatatt gagatgttta
//
