LOCUS       NM_010811               3907 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus N-deacetylase/N-sulfotransferase (heparan
            glucosaminyl) 2 (Ndst2), mRNA.
ACCESSION   NM_010811
VERSION     NM_010811.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Tao,C., Makrides,N., Chuang,J.Z., Wu,Y., Brooks,S.E., Esko,J.D.,
            Sung,C.H. and Zhang,X.
  TITLE     Chondroitin sulfate enhances the barrier function of basement
            membrane assembled by heparan sulfate
  JOURNAL   Development 149 (12) (2022)
   PUBMED   35608020
REFERENCE   3  (bases 1 to 3907)
  AUTHORS   Patel,V.N., Pineda,D.L., Berenstein,E., Hauser,B.R., Choi,S.,
            Prochazkova,M., Zheng,C., Goldsmith,C.M., van Kuppevelt,T.H.,
            Kulkarni,A., Song,Y., Linhardt,R.J., Chibly,A.M. and Hoffman,M.P.
  TITLE     Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to
            reduce epithelial morphogenesis and adult salivary gland function
  JOURNAL   Matrix Biol 103-104, 37-57 (2021)
   PUBMED   34653670
REFERENCE   4  (bases 1 to 3907)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   5  (bases 1 to 3907)
  AUTHORS   Qiu,H., Shi,S., Yue,J., Xin,M., Nairn,A.V., Lin,L., Liu,X., Li,G.,
            Archer-Hartmann,S.A., Dela Rosa,M., Galizzi,M., Wang,S., Zhang,F.,
            Azadi,P., van Kuppevelt,T.H., Cardoso,W.V., Kimata,K., Ai,X.,
            Moremen,K.W., Esko,J.D., Linhardt,R.J. and Wang,L.
  TITLE     A mutant-cell library for systematic analysis of heparan sulfate
            structure-function relationships
  JOURNAL   Nat Methods 15 (11), 889-899 (2018)
   PUBMED   30377379
REFERENCE   6  (bases 1 to 3907)
  AUTHORS   Humphries,D.E., Wong,G.W., Friend,D.S., Gurish,M.F., Qiu,W.T.,
            Huang,C., Sharpe,A.H. and Stevens,R.L.
  TITLE     Heparin is essential for the storage of specific granule proteases
            in mast cells
  JOURNAL   Nature 400 (6746), 769-772 (1999)
   PUBMED   10466726
REFERENCE   7  (bases 1 to 3907)
  AUTHORS   Aikawa,J. and Esko,J.D.
  TITLE     Molecular cloning and expression of a third member of the heparan
            sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family
  JOURNAL   J Biol Chem 274 (5), 2690-2695 (1999)
   PUBMED   9915799
REFERENCE   8  (bases 1 to 3907)
  AUTHORS   Kusche-Gullberg,M., Eriksson,I., Pikas,D.S. and Kjellen,L.
  TITLE     Identification and expression in mouse of two heparan sulfate
            glucosaminyl N-deacetylase/N-sulfotransferase genes
  JOURNAL   J Biol Chem 273 (19), 11902-11907 (1998)
   PUBMED   9565617
REFERENCE   9  (bases 1 to 3907)
  AUTHORS   Eriksson,I., Sandback,D., Ek,B., Lindahl,U. and Kjellen,L.
  TITLE     cDNA cloning and sequencing of mouse mastocytoma glucosaminyl
            N-deacetylase/N-sulfotransferase, an enzyme involved in the
            biosynthesis of heparin
  JOURNAL   J Biol Chem 269 (14), 10438-10443 (1994)
   PUBMED   8144627
REFERENCE   10 (bases 1 to 3907)
  AUTHORS   Orellana,A., Hirschberg,C.B., Wei,Z., Swiedler,S.J. and Ishihara,M.
  TITLE     Molecular cloning and expression of a glycosaminoglycan
            N-acetylglucosaminyl N-deacetylase/N-sulfotransferase from a
            heparin-producing cell line
  JOURNAL   J Biol Chem 269 (3), 2270-2276 (1994)
   PUBMED   8294485
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI173867.1, AC121599.3, AK150100.1 and BC110480.1.
            
            On Aug 24, 2006 this sequence version replaced NM_010811.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150100.1, SRR11927932.3164686.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AI173867.1         1-8
            9-220               AC121599.3         104928-105139       c
            221-584             AK150100.1         2-365
            585-667             AI173867.1         585-667
            668-2131            AK150100.1         449-1912
            2132-3654           BC110480.1         1530-3052
            3655-3907           AK150100.1         3436-3688
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 11.53 cM"
     gene            1..3907
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /note="N-deacetylase/N-sulfotransferase (heparan
                     glucosaminyl) 2"
                     /db_xref="GeneID:17423"
                     /db_xref="MGI:MGI:97040"
     exon            1..348
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            349..443
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            444..1755
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1843
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1998
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2184
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2313
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2314..2496
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2593
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2717
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2892
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..3063
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3173
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3276
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3907
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3888..3893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /note="hexamer: AATAAA"
     polyA_site      3907
                     /gene="Ndst2"
                     /gene_synonym="Mndns; NDST-2"
                     /note="major polyA site"
ORIGIN      
        1 tgccacccgg cgtggaggac ggggtccacc ctggtgggct gagcgcgagg ccggagggct
       61 gggaggctgc ggctgggctg agcactgcgg agggccgtcg gcttatccca ggtcccggtc
      121 acgcagcggc cacgctcggg gagcgcgcgg acctgagcgg gcaccacgag ccgcagcgcg
      181 ccgcctcccc ctggtgggag cggtacttga gcccgactgc ggcggagcgt tcgctcggag
      241 atggcggagc cgccgcgacg cggcccgagg ccccgcgagc agggaagccg ggcagccccg
      301 ggacgcggca gggcccgggg acagcgggga cgccgcccac tccaccgggt atgaagctaa
      361 aatacagtgg ttacaacaca ggaggttgac aggcctgacc tttgaatccc agttcatcca
      421 caaacggagc tttgtaatct cggattgtaa gggccaagct tgtggacttt agtgacttct
      481 gatctaagaa cctccaccag ggctgaggga gctcaagttt cagcctgagg catgggccag
      541 gaggccatgg tgacctgaga cccgtggatt ctgtccagta caccctgttg ctccttctgc
      601 ctcccctctc ctgtattgaa agcgtttgtg ttcatcttat cagggacctg accctaagag
      661 aatccttccc ctgccaaggc agttccagcc cccagaactc atcaccccat ctcctgaggt
      721 ggttcctatc ctccctctct tctcctcgcc atgctccagc tgtggaaggt ggtgcgccct
      781 gctcggcaac tggaactgca ccgcctcata ctgctgctga ttggtttcag tcttgtctcc
      841 atgggattcc tggcttacta cgtgtccacc agccccaagg ccaaggaacc cttgcctctg
      901 cccttgggag actgtagtag cagtggggca gctggccctg gccctgcacg gccaccagtc
      961 ccacctcgac cccaaaggcc tccagaaaca actcgaacgg aacctgtggt ccttgtgttt
     1021 gtggaaagtg catactcaca actggggcag gagattgtgg ccatcttgga atctagtcgc
     1081 tttcgttata gcactgagct agcacctggc cgaggggata tgcccacact gactgatcat
     1141 acccatggcc gctatgtctt agtcatctat gagaaccttc taaagtatgt gaacttagat
     1201 gcctggagcc gggaactgct ggaccggtac tgtgtggagt atggcgtggg tatcattggc
     1261 tttttccgag cccgcgagca cagcctgctg agtgcccagc tcaagggctt tccccttttc
     1321 ctacactcaa acctggggct ccgggactac caagtgaatc cttccgcccc tctactacat
     1381 ctcacccgcc ctagccgcct ggagccagga ccactgcctg gtgatgactg gaccatcttc
     1441 cagtccaatc acagcactta tgagcccgtg cttattgcca gccatcggcc agccgagctc
     1501 tccatgccag gaccagttct tcggcgggcc cggctcccca ccgtggtgca ggacttgggg
     1561 cttcatgatg gcatacagcg ggtgctgttt ggacatggcc tttctttttg gcttcacaaa
     1621 cttgtttttg ttgatgctgt tgcatacctc accgggaagc gcctctgcct ggaccttgac
     1681 cgctacatct tagtagatat cgatgacatc tttgtgggca aagaaggtac tcgcatgaag
     1741 gtggctgatg ttgaggcttt gttgaccact cagaacaaac tcaggacctt agtccccaac
     1801 ttcaccttca acttgggctt ctcgggcaag ttctatcata ccgggacaga agaggaggat
     1861 gctggggacg acatgctgct gaagcaccgc agggagttct ggtggttccc tcacatgtgg
     1921 agccacatgc agccacacct cttccacaac cgctctgtgc tagctgacca gatgaggctc
     1981 aacaaacagt ttgctttgga gcatgggatt cctacggatc tggggtatgc tgtggccccc
     2041 caccactctg gcgtgtaccc catccactcg cagctctatg aggcctggaa atctgtctgg
     2101 ggcatccagg tgaccagcac tgaggagtac ccccatctgc gtcctgctcg ctaccgccgt
     2161 ggcttcattc acaatggcat catggtgctc ccccggcaaa catgtggcct cttcacccac
     2221 acgatcttct ataatgagta tcctggaggt tctcgtgaac tagatcgaag catccgaggt
     2281 ggagagctct ttctgacagt gctgcttaat ccgatcagtg tctttatgac ccatctgtca
     2341 aactatggaa atgaccggct gggcctgtac acctttgaga gcctggtgcg cttcctccag
     2401 tgctggacac gactgcgcct acagaccctt cctcctgtcc ctcttgcaca aaagtacttt
     2461 gaactttttc ctcaggaacg aagccccctg tggcagaatc cctgtgatga caagaggcac
     2521 aaagatatct ggtccaagga gaaaacctgt gatcgactcc ccaagttcct cattgtggga
     2581 ccacagaaga caggcaccac ggctattcac ttcttcctga gcctgcaccc agctgtcaca
     2641 agcagttttc ccagccccag cacctttgag gagattcagt tcttcaatgg ccctaattac
     2701 cacaagggga tcgactggta catggacttc ttccctgttc cttccaatgc cagcactgac
     2761 ttcctgtttg aaaaaagtgc cacctacttt gactcggagg tcgtaccacg gcggggggct
     2821 gcccttctgc caagagccaa gataatcact gtgcttatca accctgctga cagggcctac
     2881 tcctggtacc agcaccagcg agcacatgga gaccccattg ctctgaacta caccttctac
     2941 caagtgattt cagcttcttc tcaggcccct ctgctacttc gctccttgca gaaccgttgt
     3001 cttgttcctg gctactattc tacccatctt caacgctggc tgacttacta tccctctgga
     3061 cagttgctga ttatggatgg gcaggagctt cgtgtcaacc cggcagcctc aatggagatc
     3121 atccagaagt tcctgggtat cacccccttt ctgaactaca cgaggaccct caggtttgat
     3181 gaagataaag gattttggtg ccagggcctt gaaggtggta agactcgctg tcttgggaga
     3241 agcaaaggcc ggaggtaccc agacatggac atggagtctc gccttttcct tacggatttt
     3301 ttccggaacc ataatttgga gctatcaaag ctgctgagtc ggcttggcca gccagccccc
     3361 ttgtggcttc gggaagaatt gcaacattcc agtgtgggct gatgccccag tctttcttta
     3421 ccagcaaaaa gcctgctaac ttcactgagg ggcaagtctt agagcagaac ccacaggggg
     3481 attaacatgg ccccttaccc ctctacctca gtggccccca ggattggaat ggctgaaaag
     3541 ggaggggatc catctctttc ccatagtttt gggacctaat aaaggggctt cccttggcat
     3601 ccccccatgg tactaggtac ctttgaccta tgaacctggg aggtgaggaa gagggtcctg
     3661 ggaaggggta agaagaccat attcagtggt ttccttcctc gtgcccagca gtttacctgt
     3721 caatgcctac ccctgagagg ccccttcgct atggcctctt tagtcaagac agtgggatct
     3781 acctgtggcc tggcctctct aatgtcattc acattgaatg gggatgaggt cagacagttg
     3841 ctcatagagc catatatgag ccctagctgt gggctagaaa tgtccttaat aaacatcctt
     3901 atttttc
//
