LOCUS       NM_001348100            7923 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus N-deacetylase/N-sulfotransferase (heparan
            glucosaminyl) 1 (Ndst1), transcript variant 2, mRNA.
ACCESSION   NM_001348100
VERSION     NM_001348100.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Aguilar-Calvo,P., Malik,A., Sandoval,D.R., Barback,C., Orru,C.D.,
            Standke,H.G., Thomas,O.R., Dwyer,C.A., Pizzo,D.P., Bapat,J.,
            Soldau,K., Ogawa,R., Riley,M.B., Nilsson,K.P.R., Kraus,A.,
            Caughey,B., Iliff,J.J., Vera,D.R., Esko,J.D. and Sigurdson,C.J.
  TITLE     Neuronal Ndst1 depletion accelerates prion protein clearance and
            slows neurodegeneration in prion infection
  JOURNAL   PLoS Pathog 19 (9), e1011487 (2023)
   PUBMED   37747931
  REMARK    GeneRIF: Neuronal Ndst1 depletion accelerates prion protein
            clearance and slows neurodegeneration in prion infection.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7923)
  AUTHORS   Otsuka,T., Kan,H.M., Mason,T.D., Nair,L.S. and Laurencin,C.T.
  TITLE     Overexpression of NDST1 Attenuates Fibrotic Response in Murine
            Adipose-Derived Stem Cells
  JOURNAL   Stem Cells Dev 31 (23-24), 787-798 (2022)
   PUBMED   35920108
  REMARK    GeneRIF: Overexpression of NDST1 Attenuates Fibrotic Response in
            Murine Adipose-Derived Stem Cells.
REFERENCE   4  (bases 1 to 7923)
  AUTHORS   Tao,C., Makrides,N., Chuang,J.Z., Wu,Y., Brooks,S.E., Esko,J.D.,
            Sung,C.H. and Zhang,X.
  TITLE     Chondroitin sulfate enhances the barrier function of basement
            membrane assembled by heparan sulfate
  JOURNAL   Development 149 (12) (2022)
   PUBMED   35608020
REFERENCE   5  (bases 1 to 7923)
  AUTHORS   Fan,G., Xiao,L., Cheng,L., Wang,X., Sun,B. and Hu,G.
  TITLE     Targeted disruption of NDST-1 gene leads to pulmonary hypoplasia
            and neonatal respiratory distress in mice
  JOURNAL   FEBS Lett 467 (1), 7-11 (2000)
   PUBMED   10664446
REFERENCE   6  (bases 1 to 7923)
  AUTHORS   Aikawa,J. and Esko,J.D.
  TITLE     Molecular cloning and expression of a third member of the heparan
            sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family
  JOURNAL   J Biol Chem 274 (5), 2690-2695 (1999)
   PUBMED   9915799
REFERENCE   7  (bases 1 to 7923)
  AUTHORS   Kusche-Gullberg,M., Eriksson,I., Pikas,D.S. and Kjellen,L.
  TITLE     Identification and expression in mouse of two heparan sulfate
            glucosaminyl N-deacetylase/N-sulfotransferase genes
  JOURNAL   J Biol Chem 273 (19), 11902-11907 (1998)
   PUBMED   9565617
REFERENCE   8  (bases 1 to 7923)
  AUTHORS   Shworak,N.W., Liu,J., Fritze,L.M., Schwartz,J.J., Zhang,L.,
            Logeart,D. and Rosenberg,R.D.
  TITLE     Molecular cloning and expression of mouse and human cDNAs encoding
            heparan sulfate D-glucosaminyl 3-O-sulfotransferase
  JOURNAL   J Biol Chem 272 (44), 28008-28019 (1997)
   PUBMED   9346953
REFERENCE   9  (bases 1 to 7923)
  AUTHORS   Dixon,J., Loftus,S.K., Gladwin,A.J., Scambler,P.J., Wasmuth,J.J.
            and Dixon,M.J.
  TITLE     Cloning of the human heparan
            sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher
            Collins syndrome candidate region at 5q32-q33.1
  JOURNAL   Genomics 26 (2), 239-244 (1995)
   PUBMED   7601448
REFERENCE   10 (bases 1 to 7923)
  AUTHORS   Eriksson,I., Sandback,D., Ek,B., Lindahl,U. and Kjellen,L.
  TITLE     cDNA cloning and sequencing of mouse mastocytoma glucosaminyl
            N-deacetylase/N-sulfotransferase, an enzyme involved in the
            biosynthesis of heparin
  JOURNAL   J Biol Chem 269 (14), 10438-10443 (1994)
   PUBMED   8144627
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139759.4, AK035642.1, BC079561.1, AK004787.1 and AI844370.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1-5 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784648.425869.1,
                                           SRR17784651.279143.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               AC139759.4         33266-33375         c
            111-353             AK035642.1         167-409
            354-6438            BC079561.1         1-6085
            6439-7906           AK004787.1         1471-2938
            7907-7923           AI844370.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 34.38 cM"
     gene            1..7923
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /note="N-deacetylase/N-sulfotransferase (heparan
                     glucosaminyl) 1"
                     /db_xref="GeneID:15531"
                     /db_xref="MGI:MGI:104719"
     exon            1..110
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            111..1020
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1515
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1603
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1758
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1944
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2073
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2256
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..2353
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2477
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2652
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2823
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2933
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3036
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..7907
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7885..7890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /note="hexamer: AATAAA"
     polyA_site      7907
                     /gene="Ndst1"
                     /gene_synonym="1200015G06Rik; b2b2230Clo; HSNST; HSNST 1;
                     Hsst; N-HSST; N-HSST 1; NDST-1"
                     /note="major polyA site"
ORIGIN      
        1 gcgcggccag caaggggtta agccggcccg cagcccgcct cgcgcacgcc cgggaccggc
       61 ggccgcgcgg ccacccgcgc cgcttccccc tggcgtcccg gccgaggcag catcccagcc
      121 tttaccctgt gggcccaacg tgaggagtgt gaggagcggc atgcggcgcc ccctggctgg
      181 ttcggtgtct tttgacctgc tgttgactgc tcccaggggg ccctgacgcc ctggggcact
      241 tttgctctgc acaggaccac gctggggttt gccatggtga cataaagggg tgtagaggaa
      301 ggaaggagca agaccagcct gtagactgtg cccctggctg ggcaaaaggg ccaggggccc
      361 ggagccttcc ctccactgcc ctcgaacgcc tcacttccta agtctctgca tttctcagtc
      421 agtggacagc ttttgggact cttgtgtgag gactttgggg gaccaggcca ggctgggcct
      481 ctgctgattg agatcttggg atccagaatg cctgccctgg cgtgcctccg gaggctgtgt
      541 cggcacctgt ccccacaggc tgtcctgttc ctgctgttcg tcttctgcct gttcagcgtt
      601 tttgtctctg cctactacct atatggttgg aaccggggcc ttgagccctc ggcagatgct
      661 tccgagtctg actgtgggga cccgccacct gtcgccccta gccgcctcct gccaatcaag
      721 cccgtgcagg cggttgcccc ttcccgaaca gacccactgg tgctggtatt tgtggagagc
      781 ctttattcac agctgggcca ggaggtggtg gcaatcctgg aatccagtcg cttcaaatac
      841 cggacagaga ttgcaccggg caagggggac atgcccacac tcacagacaa gggccgtggc
      901 cgcttcgccc tcatcatcta cgagaacatc ctcaagtatg tcaacctgga tgcctggaac
      961 cgggagctgc tggacaagta ctgtgtggcc tacggcgtgg gcatcattgg attcttcaag
     1021 gccaatgaga acagcctgct gagtgcacag ctcaaaggct tccctctctt cctgcattcg
     1081 aacctgggct tgaaagactg cagcatcaac cccaagtcac cactgctcta tgtgacacgg
     1141 cccagtgagg tagagaaagg cgtgctgccc ggagaggatt ggactgtgtt ccagtccaac
     1201 cattctacct acgaaccagt gctgttggcc aagacacgct cctccgaatc catcccacac
     1261 ctgggtgctg atgctggcct gcatgctgcg ctgcacgcta ctgtggtcca ggacctgggt
     1321 ctccacgatg gcatccagcg tgtgctcttt ggcaacaacc tcaacttttg gctgcataag
     1381 cttgtcttcg tggatgctgt ggccttcctc acggggaagc gcctctcact gcctttggac
     1441 cgatacatcc tggtggatat tgatgacatt tttgtgggca aggagggcac acgcatgaag
     1501 gtggaggatg tgaaggccct gtttgataca cagaatgaac tccgcacaca tattccaaac
     1561 ttcaccttca acctgggcta ctcagggaaa ttcttccaca caggtacgga tgctgaggat
     1621 gctggcgacg atctgctgtt gtcctatgtg aaagagttct ggtggttccc ccacatgtgg
     1681 agccatatgc aaccccacct cttccacaac caatctgtgc tggcagagca gatggccctg
     1741 aacaagaagt tcgctgtcga gcacggcatc cccacagata tgggctatgc ggtggcgccc
     1801 caccactccg gtgtgtaccc tgtgcacgtg cagctgtatg aggcctggaa gcaagtgtgg
     1861 ggcatccgtg tgaccagcac agaggagtac ccacatctga agccggcccg gtaccgccgt
     1921 ggctttatcc acaatggcat catggtcctc cctcggcaga cctgtggcct ctttacacac
     1981 accatcttct acaacgagta ccctggaggc tccagtgagc tggacaagat catcaatggg
     2041 ggcgagctct tcctcactgt gctcctcaat cctatcagca tcttcatgac acacttatcc
     2101 aactatggaa atgaccgcct gggactatac accttcaagc acctggtgcg cttcctgcac
     2161 tcctggacca acctgaggct gcagactctg ccccccgtgc agctggccca gaagtacttt
     2221 cagatctttt ctgaggagaa ggacccgctt tggcaggatc cctgtgagga caaacgccac
     2281 aaggacatct ggtctaagga gaagacatgt gaccgcttcc caaagcttct tatcattggc
     2341 ccccagaaaa caggcaccac ggccctctac ctgttcctgg gcatgcaccc ggacctcagc
     2401 agcaactacc ccagctccga gaccttcgag gagatccagt tttttaacgg ccacaactat
     2461 cacaaaggca tcgactggta catggagttc ttccctattc cctccaacac cacctcggac
     2521 ttctactttg agaaaagtgc caactacttt gattcagaag tggcaccacg gcgggcagct
     2581 gcactgttgc ccaaggccaa gatcctcagc atcctcatca acccagccga ccgggcttat
     2641 tcctggtacc agcaccagcg agcccatgat gacccagtgg ccctaaagta cacctttcat
     2701 gaggtgatca cagctggccc tgatgcatcc tcaaagctgc gtgccctcca gaaccggtgc
     2761 ctggtcccag gctggtatgc cactcatatt gaacgctggc tcagcgcctt tcatgccaac
     2821 cagatcctgg tcttggatgg caaactgctg cgaacagaac ctgccaaagt catggacaca
     2881 gtgcagaaat tccttggggt gaccagcact gtcgactacc ataaaacctt ggcgtttgat
     2941 ccaaagaagg gattttggtg ccaactgctt gaaggaggaa aaaccaagtg tctgggaaaa
     3001 agcaagggac ggaaatatcc agagatggac ttggattccc gagccttcct aaaggattac
     3061 ttccgggacc acaacattga gctctctaag ctgctgtata agatgggcca gacactgccc
     3121 acctggctgc gggaagacct ccagaacacc aggtagcctt ggccaccaca gccagccaga
     3181 acgcttgtgt tcgcagggat gtcctgcctc acactgagcc agactgacct gccgagaagg
     3241 aagctgggcc cagccagcca aaagcgacga gcaatactct gctgaggccc acaggggccg
     3301 gaagcccagg caggtctgca agcgcctcag agcatccacc gctggatgca tggcctgtgg
     3361 gacctctgtg gtaccagagg tccattccgt tccttcgcag cctccctgcc tggggagagc
     3421 acttcctgtt gttggaaggt catttcctgg taggaggagt cctggaggct ctctcctgtc
     3481 cctcactgtg tcctaccgtt cctgtcctgt cctttgtcac cccccctccc gctccatcag
     3541 gatgtcccct cagtattagc tgccatagtt cttctgtcct ccagacagta aaggggaaga
     3601 gcgcagctgg gcctttcgcc caaccaggga gaaagactgg gcatgtccct gagggttttg
     3661 agccaggccc tgccagggtt tagctaggca cccagatgca ctcattagac tgaatgtgag
     3721 ggtgaccatc ttgagaggac atacgactct gtatttgggt tattattatc acatctcatc
     3781 tcctctttgg gaaaagactc tctggtccct attgtatcca cctagtagtg ctcatggtct
     3841 ctcgtaggcc ctgggccact gccatgccac tgggcccaga gacgtgggcc ttggccccgt
     3901 cctgctcacc tggatgtgac atgtggtgtt tcctgtggta aaggctgagg cgagtcagga
     3961 gtctgccagt gttcatagtc ccgtgtacat agattgtttc acagctgccg cctcggccca
     4021 gcagacatgg cagtcagcaa gcagtgctgt ccgctgcttt gcctagcaac ctgtggccga
     4081 gggtccccag agacccccta acctcccaaa tactaagaag ctaaagtatt ttaatatttt
     4141 gtgttttttt tttttcttgg tgccagagtt tataccctgg gtgctggggt cgcactgtgt
     4201 tttatatata tatatatata tatgaaatgt gtatatatat attatatatt ttttttggtt
     4261 gggtttgttt ttaattcagt catatacaat gatggcaagc ccgagcctca aagaatgtcg
     4321 tatcttaatg ctagagaatg aggtggaaag gaaggtgagt gtctatgagc acccaggcct
     4381 cagggcgaag tcttgaaggg tccttcttcc ctcttctgca gagggaccag agatgctaag
     4441 atggaaagga agaccagctc tcccaaaata gtatggcttt acaaagccta gtggccgtgg
     4501 ggtgtttggg ccacagggaa aggctggccc ggggtgggac acctggggcc ctgagctggc
     4561 ctctccctcc cctgtcacat ggtgctaggt tggggtctgc tttggagtgg ggtgcccaca
     4621 gggacccaca gaactgtctc cagtccctga agacacaggg tctttggctg gtttctgttt
     4681 ggattctgtt tctgtccccc ttttaacaat gtgaagtgac tgaggttgga tttcctcttc
     4741 ctgctacccc aagcccccac atctctttaa ctgccagtcc agtgtccacc agccatttgt
     4801 tctgacatcc tggccatgca agggtcccaa gacgggcaca aagtgggtcc aggcatggga
     4861 ggtggccttg gtcaatgtca cagagctgtt ggaagcagtg ccaagagcta aattcaggcc
     4921 cctgattctg agccccgtgc tcttccagta ccacactgcc tctgagcctg ccttcgggac
     4981 cctactgtgt tccaggtccc gtctgtccct cagtcacata gctgtggtca ttccaggaca
     5041 tggggacaaa ccactctttc ccagccaggt gcctttgagc cttcccttcc aagacctgca
     5101 cttttcccgc tgtgttctct tgtttccaga gtccatccat cttactagct ggggtcgtct
     5161 tcattatgag ctattccttc ctggagttct ccactgtggt ggagagtgtg ggatgctcag
     5221 agggacctgc gtgcatggtt ttggtgtgca gagagccatg tcacatcatg tttctcactg
     5281 gggtcttgga acctgtggat cccagtccct atgtgatatg ctcaaatgcc ctgtcaacaa
     5341 gctagaacca gactcagggt taggtcagtg tgcatgccct tagccacact tcccacctgt
     5401 cagtgaggat ggctcctata ttctgaaagc tactctgaga ccctgtgaga gctgagttag
     5461 atggttggag tggcctttag atagcatgca gctctgttag tctctgcttc tggtctccat
     5521 tgaaagcaag caggtttgag accctggcag agcaggggag tggttctctc tggtccattt
     5581 ccctctaaga ccctggaaac tgctgctgtg tccctttcag tgctgggcct agaggctgcc
     5641 acaagttgca gatgtggggt cttgggaagt agatacgttc atcttagtat ctctcaatga
     5701 caatgtccac tgattgactg gaaacgtttg gaaaaacaca aaaacaagtt ggaaggaaat
     5761 tacttctgtt gttcaggcca caccctgtcc cttcttcagt ctctaccaac agcgtttgcc
     5821 ttatgggtgc agctcaccgg cagctgcctc attcattcca ggttctagag ctttctatgg
     5881 ggcgggtacc tcggtgcgca tagggaatga ggacgaagct aaaatggacc tttcaaaccc
     5941 cgatgtatga gcaatgggtg cttgtgagtc tgctcatttc cagaggagaa gtcctggcct
     6001 gccccaccct agagcccccc aggtgtgtga gtggcaagag caagctcaga accgtctcag
     6061 cgctgggctg tgactgccag ccacccttca tctagactga aggaggttag catttgccag
     6121 tgtgttttct ctcgatattc atgtacgtgt attttaaaag taagttgcag tatcatgaga
     6181 ctatttatcc ctagtgttgc cctaagaggt agggcctttt accagaccag ccagtgccac
     6241 agccaagaca gtagtcctgt cccactaaca gccattgtct agtctgtacc taaagttgtc
     6301 tcccagcccc cattctaccc cattatttta gtattggtat tggatccaat cagggcaaga
     6361 acattgtatg ttattaaaaa gtttctttga aagctaaaaa gttttccctc tgcccaactc
     6421 attaaaaaaa aaaaaaaagt ttggtttttg tttgtttgag acaaggtctc attatatagc
     6481 cctggctagc ctggaatttg ttatgtagac caggttagcc ctggaactca tagagatcca
     6541 cttaactctc aaaagctgag attaaatgtc tgcacttacc aggcccagtt caaaatggta
     6601 ttttgtttgt gtttttgaga catggtctca tcatgtaaat ctggctgtcc tggagaatgt
     6661 tatgtgaacc aggctggctt tgaactcaga gatccatctg cctctgcctc ctgagttctg
     6721 tgattaaatg tgtgtgccac ttctcccatc ccccaaatgg tattcttttt aaggccagag
     6781 ggtatgtcgt cgtctagaag tcccacattc cagatctgat tgtttcctcc tgtagctagc
     6841 ccacaggctt tcaagggtca ttttccttct tgtgtggatg tggcatttcc tcttgcctgt
     6901 agtgagcctc ctcccacagc cccccaagtg agggccaaac cagcttgggg gaggggaagg
     6961 ggctccccaa agaggcaaca tcacatgggt aggggttgag ggctctgtgg gatgggatgg
     7021 tcctgctgga ctgaccctgg agggccaggc taggcacagc ttagaggagc ccggctgccc
     7081 catgctaagc acagcattgg tctttctcca tctcttccct cttctgtctg tgttttggtt
     7141 tccctgcttc tgttctgtgg tcctggggtc ttcattctct agatggtcag tgtgcactgg
     7201 aacagagatg agacctcaag ctatgacagg gaaggcctag gagaaaggcg tcctctaggc
     7261 ggaggcctga gaagagcacc gggtgcagca ggcggtcacg tgacggtctt cctccataaa
     7321 tgagatggag gcttcagcag gggtttcaca tccacgcaag aggcagctgt gctctgtgtg
     7381 atctgaggct aggagtctgc ctcctcccct tccaccccca ggccttccat gcagtgctgg
     7441 ggttagggtt gtctggctgt gggcttgata tccttgccag ctctgagtag cccggcaatg
     7501 tctgagggcc agcgggaagg ccaggggaga ctccctgaaa tggttcacct aagaagcttc
     7561 cctcaacctc tccctggcct ggaggaggat cccagaggaa agggttttct atgactgagc
     7621 tagcttttct tacgggcaag agggctcttt ttgtaatttg tgcatgaaat tcatgttatt
     7681 tcctggggag agaagagggc caactgagtg ggtggggggc acgtgtggga agcaggcaga
     7741 ggtttccttc cctggagggg tggggagggc tcgcagaggg catgctacct agctcacctg
     7801 aagagggctc ttgaaccagc cgttttgggt tgtgttaaat gtgggaactt tcctccatta
     7861 atgtacaatc ttgaactaac tgctaataaa atgggattct gtttgtaaaa aaaaaaaaaa
     7921 aaa
//
