LOCUS       NM_013865               2644 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus N-myc downstream regulated gene 3 (Ndrg3), transcript
            variant 2, mRNA.
ACCESSION   NM_013865
VERSION     NM_013865.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Koo,H., Park,K.C., Sohn,H.A., Kang,M., Kim,D.J., Park,Z.Y.,
            Park,S., Min,S.H., Park,S.H., You,Y.M., Han,Y., Kim,B.K., Lee,C.H.,
            Kim,Y.S., Chung,S.J., Yeom,Y.I. and Lee,D.C.
  TITLE     Anti-proteolytic regulation of KRAS by USP9X/NDRG3 in KRAS-driven
            cancer development
  JOURNAL   Nat Commun 16 (1), 628 (2025)
   PUBMED   39819877
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2644)
  AUTHORS   Xu,Y., Kusuyama,J., Osana,S., Matsuhashi,S., Li,L., Takada,H.,
            Inada,H. and Nagatomi,R.
  TITLE     Lactate promotes neuronal differentiation of SH-SY5Y cells by
            lactate-responsive gene sets through NDRG3-dependent and
            -independent manners
  JOURNAL   J Biol Chem 299 (6), 104802 (2023)
   PUBMED   37172727
  REMARK    GeneRIF: Lactate promotes neuronal differentiation of SH-SY5Y cells
            by lactate-responsive gene sets through NDRG3-dependent and
            -independent manners.
REFERENCE   4  (bases 1 to 2644)
  AUTHORS   Sohn,H.A., Lee,D.C., Park,A., Kang,M., Yoon,B.H., Lee,C.H.,
            Kim,Y.H., Oh,K.J., Kim,C.Y., Park,S.H., Koo,H., Kim,H.C.,
            Yoon,W.K., Lim,D.S., Kim,D., Park,K.C. and Yeom,Y.I.
  TITLE     Glycogen Storage Disease Phenotypes Accompanying the Perturbation
            of the Methionine Cycle in NDRG3-Deficient Mouse Livers
  JOURNAL   Cells 11 (9), 1536 (2022)
   PUBMED   35563842
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2644)
  AUTHORS   Nakano,Y., Wiechert,S. and Banfi,B.
  TITLE     Overlapping Activities of Two Neuronal Splicing Factors Switch the
            GABA Effect from Excitatory to Inhibitory by Regulating REST
  JOURNAL   Cell Rep 27 (3), 860-871 (2019)
   PUBMED   30995482
REFERENCE   6  (bases 1 to 2644)
  AUTHORS   Jerez-Timaure,N.C., Eisen,E.J. and Pomp,D.
  TITLE     Fine mapping of a QTL region with large effects on growth and
            fatness on mouse chromosome 2
  JOURNAL   Physiol Genomics 21 (3), 411-422 (2005)
   PUBMED   15769905
REFERENCE   7  (bases 1 to 2644)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 2644)
  AUTHORS   Qu,X., Zhai,Y., Wei,H., Zhang,C., Xing,G., Yu,Y. and He,F.
  TITLE     Characterization and expression of three novel
            differentiation-related genes belong to the human NDRG gene family
  JOURNAL   Mol Cell Biochem 229 (1-2), 35-44 (2002)
   PUBMED   11936845
REFERENCE   9  (bases 1 to 2644)
  AUTHORS   Zhao,W., Tang,R., Huang,Y., Wang,W., Zhou,Z., Gu,S., Dai,J.,
            Ying,K., Xie,Y. and Mao,Y.
  TITLE     Cloning and expression pattern of the human NDRG3 gene
  JOURNAL   Biochim Biophys Acta 1519 (1-2), 134-138 (2001)
   PUBMED   11406283
REFERENCE   10 (bases 1 to 2644)
  AUTHORS   Okuda,T. and Kondoh,H.
  TITLE     Identification of new genes ndr2 and ndr3 which are related to
            Ndr1/RTP/Drg1 but show distinct tissue specificity and response to
            N-myc
  JOURNAL   Biochem Biophys Res Commun 266 (1), 208-215 (1999)
   PUBMED   10581191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY125191.1, AK004689.1 and AV032677.1.
            
            On Aug 13, 2009 this sequence version replaced NM_013865.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            lacks an alternate in-frame exon in the 3' coding region compared
            to variant 3. The resulting isoform (2) has the same N- and
            C-termini but is shorter compared to isoform 1. Variants 2 and 4
            both encode the same isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154927.1, SRR1660819.183864.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                BY125191.1         2-56
            56-2640             AK004689.1         2-2586
            2641-2644           AV032677.1         203-206
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 77.89 cM"
     gene            1..2644
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /note="N-myc downstream regulated gene 3"
                     /db_xref="GeneID:29812"
                     /db_xref="MGI:MGI:1352499"
     exon            1..113
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            114..215
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..1286
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /note="isoform 2 is encoded by transcript variant 2; N-myc
                     downstream regulated 3; protein NDRG3; protein Ndr3; N-myc
                     downstream-regulated gene 3 protein"
                     /codon_start=1
                     /product="protein NDRG3 isoform 2"
                     /protein_id="NP_038893.1"
                     /db_xref="CCDS:CCDS16971.1"
                     /db_xref="GeneID:29812"
                     /db_xref="MGI:MGI:1352499"
                     /translation="MDELQDVQLTEIKPLLNDKNGTRNFQDFDCQEHDIETPHGMVHV
                     TIRGLPKGNRPVILTYHDIGLNHKSCFNTFFNFEDMQEITQHFAVCHVDAPGQQEAAP
                     SFPTGYQYPTMDELAEMLPPVLTHLSMKSIIGIGVGAGAYILSRFALNHPELVEGLVL
                     INIDPCAKGWIDWAASKLSGFTTNIVDIILAHHFGQEELQANLDLIQTYRLHIAQDIN
                     QENLQLFLGSYNGRRDLEIERPILGQNDNRLKTLKCSTLLVVGDNSPAVEAVVECNSR
                     LDPINTTLLKMADCGGLPQVVQPGKLTEAFKYFLQGMGYIPSASMTRLARSRTHSTSS
                     SIGSGESPFSRSVTSNQSDGTQESCESPDVLDRHQTMEVSC"
     exon            216..251
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            252..357
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            358..478
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            479..541
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            542..602
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            603..689
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            690..746
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            747..850
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            851..916
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            917..968
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1016
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1052
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1104
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..2644
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2615..2620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /note="hexamer: AATAAA"
     polyA_site      2641
                     /gene="Ndrg3"
                     /gene_synonym="4833415O14Rik; Ndr3"
                     /note="major polyA site"
ORIGIN      
        1 atcctcagcg cgcctgcgct gcgcagcgcc ccccctcctt gctgctgctg ctgctgctgc
       61 tgctgctgct gccgctgctg ctgtagattg acggccccgc gctagcgctt cagagttcct
      121 ggttcatcct tgagactcat ctgctctgag ccatcctcat ggatgaactt caggatgttc
      181 aactcacaga gatcaaaccg cttctaaatg ataagaatgg cacacgaaac ttccaggact
      241 ttgactgtca ggaacatgac attgaaacac cgcatggtat ggtccacgtc accataagag
      301 gcttaccaaa aggaaacaga cctgttatcc tgacatacca cgacattggg ctcaaccata
      361 agtcctgctt caacacattc ttcaactttg aggatatgca agagatcacc cagcactttg
      421 ctgtctgtca cgtggacgct cctggccaac aagaagcagc accctccttc cccacagggt
      481 accagtaccc caccatggat gagctagctg agatgctgcc tcctgttctc acacacttaa
      541 gtatgaagag catcattggt attggggttg gagcaggagc ctacattctc agcagatttg
      601 cactgaatca tccagagctg gtggaaggtc ttgtgctcat taacattgac ccgtgtgcta
      661 agggctggat tgactgggca gcttccaaac tctcaggttt taccaccaac attgtggaca
      721 taattctggc tcatcacttt ggccaggagg agttgcaggc caatttggac ctcattcaaa
      781 cctacagact gcacattgct caagatatca accaagaaaa cctccagctc ttcctgggtt
      841 cctataatgg gcgcagagac ctggagatcg aaaggcctat actgggccaa aatgataaca
      901 gattgaaaac gctaaagtgt tctaccttac tggtggtggg ggacaattca cctgctgtgg
      961 aggctgtggt tgaatgtaat tctcgcctcg accctataaa tacaactttg ctaaagatgg
     1021 cagactgtgg aggactgccc caagtagttc agcctgggaa gctcaccgag gccttcaagt
     1081 actttttgca gggaatgggc tacataccat ctgccagcat gacccggctc gcccgatcac
     1141 gaacccactc cacctcaagt agcattggct ctggagaaag tccctttagc aggtctgtga
     1201 ccagcaatca gtcagatgga actcaagaat cctgtgagtc ccctgatgtc ctggacagac
     1261 accagaccat ggaagtgtct tgctaagcag atctcctccc ctggaccatg caagtccctc
     1321 tccttgaaat gaccactccg taatagaaca tttcattccg tcagctggcc cccacttcct
     1381 ttcactcacg catggccaca gcatctctct ctagtagccg agagtgtcat tctctccacc
     1441 ctttctgtgt gtaccagagc cacacccatg ccattactgt aacattccaa catctccatc
     1501 tgacctgacc tccatatctt ctcttgccag cctttcccta tgctccctca aatatgtatc
     1561 tgatgagatt ctttaattcc caactccatg tgtgtgcgag cacgcgtgtc tgtgtctgtg
     1621 tgtgcatact tctgtgactt tagacatgct ttcttgtagt gcttctgcaa agggactttt
     1681 tttgaatcct cagtggcatt tttttaaggg aaatatgcag agtggatatc tgggtgggtt
     1741 gggccacaag attggatttt ggttccactt ggacaacaaa cagtatttgc agagtgattt
     1801 caggagagag caggtctgag gaatatgcaa aactgtagtt actgtgtggg ccattgactg
     1861 ccttgaccag tagaagggat aactattaca cagataggca ggcagggtgg cagtcacctc
     1921 ccctgtccac gtggatccat ccctgccatt gtgtgagtca gagggataga tctgaagcct
     1981 gtgaatgatg tctggtacac cacagtgttt tctccagaga actggcctaa gctctagatt
     2041 agccaagaaa gctggaaaac agagcgccag gcttcactct gcagagagaa agtgctctgt
     2101 ccctgtagcg tctgattcca cttaccagac acccacgcat ctgcaggatg tctgatgcag
     2161 agcggttctc catccctcag ttccagaaaa aatggagtga tatttaaaaa tgccatcgat
     2221 ggtggggatc ttactgggga agaggaagga ccattagcac accatcatga tgtcagatga
     2281 caaaatggaa gccaagacac cttgaaggtg actttctagg aaggtcttaa gcatgtaatg
     2341 tccctttatc agagggaagg ggacaaactc agggcagccc tgtccaggta gaaatatttt
     2401 tgcccccctg tctgatgttg atgaggggtc ataccagcca gggagaccct ctgggaggaa
     2461 gctgccacac acaaggactc tggaagtatc cagatgtgag cccagccagg gtcctatggt
     2521 tccaaatctg aagaaaaggt ttttcacaca ctccttgctt tctgctaaga taagaaaggc
     2581 gtcactctgc cagagtgtga ctttttacag attaaataaa gctgtatatg tctcattgtt
     2641 accc
//
