LOCUS       NM_029561               2482 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus Nedd4 family interacting protein 2 (Ndfip2),
            transcript variant 1, mRNA.
ACCESSION   NM_029561 XM_001005690
VERSION     NM_029561.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Gorla,M., Santiago,C., Chaudhari,K., Layman,A.A.K., Oliver,P.M. and
            Bashaw,G.J.
  TITLE     Ndfip Proteins Target Robo Receptors for Degradation and Allow
            Commissural Axons to Cross the Midline in the Developing Spinal
            Cord
  JOURNAL   Cell Rep 26 (12), 3298-3312 (2019)
   PUBMED   30893602
REFERENCE   3  (bases 1 to 2482)
  AUTHORS   Trimpert,C., Wesche,D., de Groot,T., Pimentel Rodriguez,M.M.,
            Wong,V., van den Berg,D.T.M., Cheval,L., Ariza,C.A., Doucet,A.,
            Stagljar,I. and Deen,P.M.T.
  TITLE     NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and
            degradation
  JOURNAL   PLoS One 12 (9), e0183774 (2017)
   PUBMED   28931009
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2482)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2482)
  AUTHORS   O'Leary,C.E., Riling,C.R., Spruce,L.A., Ding,H., Kumar,S., Deng,G.,
            Liu,Y., Seeholzer,S.H. and Oliver,P.M.
  TITLE     Ndfip-mediated degradation of Jak1 tunes cytokine signalling to
            limit expansion of CD4+ effector T cells
  JOURNAL   Nat Commun 7, 11226 (2016)
   PUBMED   27088444
  REMARK    GeneRIF: Data indicate thar Janus kinase 1 (Jak1) degradation is
            dependent on Nedd4 family interacting proteins Ndfip1/Ndfip2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2482)
  AUTHORS   Foot,N.J., Dalton,H.E., Shearwin-Whyatt,L.M., Dorstyn,L., Tan,S.S.,
            Yang,B. and Kumar,S.
  TITLE     Regulation of the divalent metal ion transporter DMT1 and iron
            homeostasis by a ubiquitin-dependent mechanism involving Ndfips and
            WWP2
  JOURNAL   Blood 112 (10), 4268-4275 (2008)
   PUBMED   18776082
  REMARK    GeneRIF: DMT1 interacts with 2 WW domain-interacting proteins,
            Ndfip1 and Ndfip2. This promotes DMT1 ubiquitination and
            degradation by WWP2.
REFERENCE   7  (bases 1 to 2482)
  AUTHORS   Shearwin-Whyatt,L.M., Brown,D.L., Wylie,F.G., Stow,J.L. and
            Kumar,S.
  TITLE     N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to
            multivesicular bodies and the Golgi, and has a potential role in
            protein trafficking
  JOURNAL   J Cell Sci 117 (Pt 16), 3679-3689 (2004)
   PUBMED   15252135
REFERENCE   8  (bases 1 to 2482)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2482)
  AUTHORS   Konstas,A.A., Shearwin-Whyatt,L.M., Fotia,A.B., Degger,B.,
            Riccardi,D., Cook,D.I., Korbmacher,C. and Kumar,S.
  TITLE     Regulation of the epithelial sodium channel by N4WBP5A, a novel
            Nedd4/Nedd4-2-interacting protein
  JOURNAL   J Biol Chem 277 (33), 29406-29416 (2002)
   PUBMED   12050153
REFERENCE   10 (bases 1 to 2482)
  AUTHORS   Harvey,K.F., Shearwin-Whyatt,L.M., Fotia,A., Parton,R.G. and
            Kumar,S.
  TITLE     N4WBP5, a potential target for ubiquitination by the Nedd4 family
            of proteins, is a novel Golgi-associated protein
  JOURNAL   J Biol Chem 277 (11), 9307-9317 (2002)
   PUBMED   11748237
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC141878.4.
            
            On Feb 16, 2019 this sequence version replaced NM_029561.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC071237.1, AK132235.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-380               AC141878.4         37170-37549
            381-543             AC141878.4         66163-66325
            544-677             AC141878.4         70778-70911
            678-771             AC141878.4         73345-73438
            772-896             AC141878.4         76549-76673
            897-963             AC141878.4         80845-80911
            964-1069            AC141878.4         83277-83382
            1070-2482           AC141878.4         86432-87844
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 55.64 cM"
     gene            1..2482
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="Nedd4 family interacting protein 2"
                     /db_xref="GeneID:76273"
                     /db_xref="MGI:MGI:1923523"
     exon            1..380
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1067
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="NEDD4 WW domain-binding protein 5A"
                     /codon_start=1
                     /product="NEDD4 family-interacting protein 2"
                     /protein_id="NP_083837.2"
                     /db_xref="CCDS:CCDS49560.2"
                     /db_xref="GeneID:76273"
                     /db_xref="MGI:MGI:1923523"
                     /translation="MGRRRSQRACASAPSMLSSARGSPELRRRSASDAELSAGAEGAT
                     GSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQ
                     LGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEA
                     PTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPR
                     DDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIK
                     WILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRY
                     FFLL"
     exon            381..543
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            544..677
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            678..771
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            772..896
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            897..963
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1069
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..2482
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1966..1971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="hexamer: AATAAA"
     polyA_site      1987
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="hexamer: AATAAA"
     polyA_site      2055
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="major polyA site"
     regulatory      2461..2466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
                     /note="hexamer: AATAAA"
     polyA_site      2482
                     /gene="Ndfip2"
                     /gene_synonym="0710001O20Rik; 2810436B12Rik;
                     9130207N19Rik; mKIAA1165; N4wbp5a"
ORIGIN      
        1 gactcttcca tctcctccca cctagggaca cctccggagc cgggcggcgg atgggacgcc
       61 ggcggagcca gagagcatgc gcgtccgcgc cgagcatgct cagtagcgcg cgcggctccc
      121 cggagctccg gcgccgatcg gcgagcgacg cggagctctc cgctggagcc gagggagcga
      181 caggaagtga agcggccccg ccaggcgacc tcggcggcag aacccgaggc ggcggaagag
      241 gctctgcggc ggcggcggcg acgacgtcga cccgggaggc cgagggagct gagcgccgag
      301 gggacacgcc cgctcggaag ccggaccccg aggcgggcag gatggatcac caccagctgg
      361 ggactggtcg ctaccaggtg cttcacaatg aagaagataa ctcagaatca tctgctgtgg
      421 agcagccgtc aacttctagc ttagcagcgc cgactgtgga ggctgccgct tcagcaccag
      481 cactggaccc tgactctcct cctccttaca gcagcatcac tgtggaagct cctaccactt
      541 cagatactga cgtgtatagt gagttttacc ctgtgccacc tccgtacagt gttgctacct
      601 ctctgcctac atatgacgaa gctgaaaagg ctaaagctgc tgccctggca gctgctgcag
      661 cagatgcacc tcagagaaac caagaggaag actgtacgcc acgggatgac ttcagtgatg
      721 tagagcagct cagagtgggc aacgacggca ttttcatgct ggcctttttc atggcattca
      781 tattcaactg gcttgggttc tgcttatcct tctgtatcac caataccatc gctggaagat
      841 acggtgctat ctgtgggttt ggcctttcac tgatcaaatg gatccttatt gtcaggtttt
      901 ctgactattt tactggctat ttcaatggac agtattggct ttggtggata tttctcgtac
      961 ttggcctgct ccttttcttc agagggtttg ttaattacct aaaagtcaga aacatgtctg
     1021 aaagtatggc ggctgcacat aggacgaggt acttcttcct gctatagagg tggcatcaac
     1081 ccatcattcc tttcttacac caatgtgaac cgtccacatc atctgtaaag cgacagcttt
     1141 gctaagatag aagactgctg agagcagaag gaaatgtagt gaagggtgga gcttcggaat
     1201 taaatgacag ggtggtttac gctaaaagcc atttctgttc attccttaat ctgtgtattt
     1261 catttatttt gcacatttgc atatgtgctt gtcttacaga tatgcatata cttgaaagaa
     1321 tctataacgt tgtagcagca gagcccagtc ctgctcggtt actcagtgct gatacaattg
     1381 gaggaattgg gtaacgagca gccttccagc acgtgacgct tccgacgtgg ccgttagcgt
     1441 aatcagagtt acactattac tcttgtccag tgctttactt tctgcctctt agatgcatct
     1501 agtaatccac acacgaaata ttctttgtta tatatgatgt ttattaaaac ttgtggtgtg
     1561 cttattagaa agaatttgtc agatttttga atatgatatt atttaggaaa tctgtaaata
     1621 accatgcata tattgctttt tacattgttt tcttaaattg tgtctagata tcttttcatt
     1681 aattttaaat taagtaaact aaataaataa aaataaactt ttttaaagat gtgtggttta
     1741 ggatgggctt taagaaagtg agtctaccaa atgccagtta aagagcaagt taggggtacg
     1801 ccagtttcag gggtgttcag atgagaacct taggttttaa gattactcac cttacagttc
     1861 ttataagaag agattgcttt agatcttata tttatttaca tttaaaacaa tttaaaaatt
     1921 aatttaccaa atatatcctc atttgcattt gtcttttaaa aagccaataa agatatcttt
     1981 cagagtatta ctacaatcat ttttctttaa atgtttaatt tgttaagtgt agaaaataaa
     2041 atcttgttct gttaatagtg cctcgtttag tgattccaag actaatttgt aaaaacgtac
     2101 agcctgaatt gagatcctca gaatacctga tttcatgttg ttgaaatgtc tctatagaat
     2161 gaatttaggg tgtggagtgt gtgtggtgag tttgtgtggc cattactcca actggaatct
     2221 gcttttacat caaaagctac aatgctagcc atctcacaga tcattttctg acttttgtgg
     2281 ttttcatttt atcttaactt atgaatgcta aaagtaaaag atgatggaaa ttacactgct
     2341 gcttacatat aaaaaaaaaa acatttaaca tagccgtaaa gaacatacaa aattgattgc
     2401 aacactatga ctaaaaagtg catctaactc ttcagggctg tttgtcccat cttaaggaaa
     2461 aataaaattt attactttat aa
//
