LOCUS       NM_023294               2201 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus NDC80 kinetochore complex component (Ndc80), mRNA.
ACCESSION   NM_023294
VERSION     NM_023294.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   He,S., Gillies,J.P., Zang,J.L., Cordoba-Beldad,C.M., Yamamoto,I.,
            Fujiwara,Y., Grantham,J., DeSantis,M.E. and Shibuya,H.
  TITLE     Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate
            mitotic and male meiotic spindle bipolarity
  JOURNAL   Nat Commun 14 (1), 1715 (2023)
   PUBMED   36973253
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2201)
  AUTHORS   Li,Y., Yuan,J., Chen,F., Zhang,S., Zhao,Y., Chen,X., Lu,L.,
            Zhou,L., Chu,C.Y., Sun,H. and Wang,H.
  TITLE     Long noncoding RNA SAM promotes myoblast proliferation through
            stabilizing Sugt1 and facilitating kinetochore assembly
  JOURNAL   Nat Commun 11 (1), 2725 (2020)
   PUBMED   32483152
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2201)
  AUTHORS   Yoshida,S., Nishiyama,S., Lister,L., Hashimoto,S., Mishina,T.,
            Courtois,A., Kyogoku,H., Abe,T., Shiraishi,A., Choudhary,M.,
            Nakaoka,Y., Herbert,M. and Kitajima,T.S.
  TITLE     Prc1-rich kinetochores are required for error-free acentrosomal
            spindle bipolarization during meiosis I in mouse oocytes
  JOURNAL   Nat Commun 11 (1), 2652 (2020)
   PUBMED   32461611
  REMARK    GeneRIF: Prc1-rich kinetochores are required for error-free
            acentrosomal spindle bipolarization during meiosis I in mouse
            oocytes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2201)
  AUTHORS   Makita,Y., Murata,S., Katou,Y., Kikuchi,K., Uejima,H., Teratani,M.,
            Hoashi,Y., Kenjo,E., Matsumoto,S., Nogami,M., Otake,K. and
            Kawamata,Y.
  TITLE     Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice
            of hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 493 (1), 800-806 (2017)
   PUBMED   28843857
  REMARK    GeneRIF: These data strongly indicated that KNTC2 is a promising
            target for the treatment of HCC and that phosphorylated HH3 at
            serine 10 is one of the target engagement markers for KNTC2.
REFERENCE   6  (bases 1 to 2201)
  AUTHORS   Ciferri,C., Pasqualato,S., Screpanti,E., Varetti,G., Santaguida,S.,
            Dos Reis,G., Maiolica,A., Polka,J., De Luca,J.G., De Wulf,P.,
            Salek,M., Rappsilber,J., Moores,C.A., Salmon,E.D. and Musacchio,A.
  TITLE     Implications for kinetochore-microtubule attachment from the
            structure of an engineered Ndc80 complex
  JOURNAL   Cell 133 (3), 427-439 (2008)
   PUBMED   18455984
REFERENCE   7  (bases 1 to 2201)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 2201)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   9  (bases 1 to 2201)
  AUTHORS   Wigge,P.A. and Kilmartin,J.V.
  TITLE     The Ndc80p complex from Saccharomyces cerevisiae contains conserved
            centromere components and has a function in chromosome segregation
  JOURNAL   J Cell Biol 152 (2), 349-360 (2001)
   PUBMED   11266451
REFERENCE   10 (bases 1 to 2201)
  AUTHORS   Chen,Y., Riley,D.J., Chen,P.L. and Lee,W.H.
  TITLE     HEC, a novel nuclear protein rich in leucine heptad repeats
            specifically involved in mitosis
  JOURNAL   Mol Cell Biol 17 (10), 6049-6056 (1997)
   PUBMED   9315664
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF326730.1, BX527376.1, AK011497.1 and CJ201671.1.
            
            On Dec 10, 2008 this sequence version replaced NM_023294.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF326730.1, AK011497.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AF326730.1         15-24
            11-150              BX527376.1         12-151
            151-2199            AK011497.1         121-2169
            2200-2201           CJ201671.1         400-401
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 41.87 cM"
     gene            1..2201
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /note="NDC80 kinetochore complex component"
                     /db_xref="GeneID:67052"
                     /db_xref="MGI:MGI:1914302"
     exon            1..147
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            148..257
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..2085
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /note="highly expressed in cancer, rich in leucine heptad
                     repeats; kinetochore associated 2; kinetochore protein
                     Hec1; kinetochore-associated protein 2; NDC80 homolog,
                     kinetochore complex component"
                     /codon_start=1
                     /product="kinetochore protein NDC80 homolog"
                     /protein_id="NP_075783.2"
                     /db_xref="CCDS:CCDS28959.1"
                     /db_xref="GeneID:67052"
                     /db_xref="MGI:MGI:1914302"
                     /translation="MKRSSVSTCGAGRLSMQELRTLDLNKPGLYTPQTKERSTFGKLS
                     THKPTSERKVSIFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEF
                     LTENGYVYSVSMKSLQAPSTKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGY
                     PFTLSKSSMYTVGAPHTWPHIVAALVWLIDCIKIDTAMKESSPLFDDGQLWGEETEDG
                     IKHNKLFLEYTKKCYEKFMTGADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALN
                     EQIARLEEEREREPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEE
                     IGRVEQECETVKQENTRLQSIVDNQKYSVADIERINHEKNELQQTINKLTKDLEAEQQ
                     QMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCL
                     VKYRTQVYAPLKELLNESEEEINKALNKKRHLEDTLEQLNTMKTESKNTVRMLKEEIQ
                     KLDDLHQQAVKEAEEKDKKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQL
                     TVKTTTEERRKVENNLQRLLEMVATHVGSLEKHLEEENAKADREYEEFMSEDLLENIR
                     EMAEKYKRNAAQLKAPDK"
     exon            258..335
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..459
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            460..632
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            633..735
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            736..825
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            826..919
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1026
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1171
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1377
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1530
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1620
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1713
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1844
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1947
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2201
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2178..2183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /note="hexamer: AATAAA"
     polyA_site      2199
                     /gene="Ndc80"
                     /gene_synonym="2610020P18Rik; HEC; HEC1; Kntc2"
                     /note="major polyA site"
ORIGIN      
        1 tgacgtcagc gcgggcggaa actttaaaat tcaaaccgcc ttgggttgac ggaagcggac
       61 gctctggttt gggcggctgt tttcgtgtgc agtcgtgcgg tttacggaaa gctgggcgcc
      121 gggtctcgtt gagaagctga gcgaaaggtt tcgagaatga agcgcagttc agtttccacc
      181 tgtggtgctg gccgcctctc tatgcaggag ttaaggaccc tggacctcaa taagccaggc
      241 ctttataccc ctcaaaccaa agaaagatca acctttggaa agctgagtac acacaaaccg
      301 acatcggaaa gaaaagtctc aatatttggg aaaaggacta gcggacatgg atccaggaat
      361 agtcaacttg gtatattttc cagttctgaa aaaatcaagg acccaagacc acttaatgac
      421 aaagcattca ttcagcagtg tattcgacaa ctctatgagt ttcttacaga aaacggttat
      481 gtgtatagtg tatccatgaa gtctctgcaa gctccatcca ctaaagagtt cctaaagatc
      541 ttcgcctttc tttatggctt tctgtgcccg tcgtatgaac ttcctggtac aaaatgtgaa
      601 gaagaggtcc caagaatttt taaagcactt gggtatccct tcacactgtc caagagctcc
      661 atgtatacag tgggagcccc tcacacgtgg cctcacatcg tggctgcctt ggtgtggctc
      721 atagactgca tcaagattga tactgccatg aaagaaagct cacctttatt tgatgatggg
      781 cagctctggg gagaagagac tgaagatgga attaaacaca ataagttgtt tttggagtac
      841 accaaaaagt gctatgagaa gttcatgacc ggggccgaca gctttgaaga agaggatgct
      901 gagctgcagg cgaagctgaa ggacttgtac aaggtagatg cgtctaagct ggagtcactc
      961 gaagcagaaa acaaagcact aaatgaacag attgcaagac tggaggagga aagagaaaga
     1021 gaaccgaacc gtctgatgtc attgaagaaa ctgaaagcgt ccttacaagc agatgttcaa
     1081 aactataaag catacatgag caacttggag tctcatttag ccgtcctgaa acagaaatcg
     1141 aatagtcttg atgaagaaat tggtagagta gaacaagaat gtgaaactgt taaacaggaa
     1201 aacactcgac tacagagtat cgttgataac cagaagtatt cagtcgctga cattgagaga
     1261 ataaatcatg agaaaaatga attgcagcag actattaata aattaaccaa agacctggaa
     1321 gccgaacagc aacagatgtg gaatgaagaa ttaaaatacg caagaggcaa agaggcgatt
     1381 gaagcgcagc tagcggagta ccacaagttg gctagaaaat taaagcttat ccccaaaggt
     1441 gctgagaatt ccaaaggtta cgactttgaa attaagttta atcctgaggc gggtgccaac
     1501 tgccttgtca aatacaggac tcaagtgtat gcaccgctca aagagctctt gaatgaaagc
     1561 gaagaagaaa ttaacaaagc tctgaataaa aagaggcatc tggaggatac tttagaacaa
     1621 ctgaacacca tgaaaacgga aagcaagaac actgtgagga tgctgaagga ggagattcag
     1681 aaactggatg accttcacca gcaggcagtg aaggaagctg aggaaaaaga caagaagagt
     1741 gccagtgagc ttgagtccct ggagaaacac aagcacctgc tggagagcgg ggtgaacgat
     1801 ggcctcagcg aggccatgga tgagttggac gccgtccagc gggaatacca gctaactgtg
     1861 aagaccacaa ctgaagaaag aagaaaggtg gaaaacaact tacaacgtct tttggagatg
     1921 gtcgccacac acgtagggtc tttggagaaa catcttgaag aggagaatgc taaagccgac
     1981 agagagtatg aagaattcat gtctgaagat ctcctggaaa acatcaggga gatggcagag
     2041 aagtataaga gaaatgctgc ccaacttaag gctcccgaca aatgaagata aactaatctt
     2101 tcatagatat aacatagtat tttatcccca aattatgata atatggtaaa ttgacctttt
     2161 agacagatca gacagttaat aaagtatact tgtaaagtgt g
//
