LOCUS       NM_153505               4717 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus NCK associated protein 1 like (Nckap1l), mRNA.
ACCESSION   NM_153505
VERSION     NM_153505.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Wang,X., Shao,L., Richardson,K.K., Ling,W., Warren,A., Krager,K.,
            Aykin-Burns,N., Hromas,R., Zhou,D., Almeida,M. and Kim,H.N.
  TITLE     Hematopoietic cytoplasmic adaptor protein Hem1 promotes osteoclast
            fusion and bone resorption in mice
  JOURNAL   J Biol Chem 299 (2), 102841 (2023)
   PUBMED   36574841
  REMARK    GeneRIF: Hematopoietic cytoplasmic adaptor protein Hem1 promotes
            osteoclast fusion and bone resorption in mice.
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Avalos,A., Tietsort,J.T., Suwankitwat,N., Woods,J.D., Jackson,S.W.,
            Christodoulou,A., Morrill,C., Liggitt,H.D., Zhu,C., Li,Q.Z.,
            Bui,K.K., Park,H. and Iritani,B.M.
  TITLE     Hem-1 regulates protective humoral immunity and limits autoantibody
            production in a B cell-specific manner
  JOURNAL   JCI Insight 7 (9), e153597 (2022)
   PUBMED   35531955
  REMARK    GeneRIF: Hem-1 regulates protective humoral immunity and limits
            autoantibody production in a B cell-specific manner.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4717)
  AUTHORS   Stahnke,S., Doring,H., Kusch,C., de Gorter,D.J.J., Dutting,S.,
            Guledani,A., Pleines,I., Schnoor,M., Sixt,M., Geffers,R., Rohde,M.,
            Musken,M., Kage,F., Steffen,A., Faix,J., Nieswandt,B., Rottner,K.
            and Stradal,T.E.B.
  TITLE     Loss of Hem1 disrupts macrophage function and impacts migration,
            phagocytosis, and integrin-mediated adhesion
  JOURNAL   Curr Biol 31 (10), 2051-2064 (2021)
   PUBMED   33711252
  REMARK    GeneRIF: Loss of Hem1 disrupts macrophage function and impacts
            migration, phagocytosis, and integrin-mediated adhesion.
REFERENCE   4  (bases 1 to 4717)
  AUTHORS   Suwankitwat,N., Libby,S., Liggitt,H.D., Avalos,A., Ruddell,A.,
            Rosch,J.W., Park,H. and Iritani,B.M.
  TITLE     The actin-regulatory protein Hem-1 is essential for alveolar
            macrophage development
  JOURNAL   J Exp Med 218 (4) (2021)
   PUBMED   33600594
  REMARK    GeneRIF: The actin-regulatory protein Hem-1 is essential for
            alveolar macrophage development.
REFERENCE   5  (bases 1 to 4717)
  AUTHORS   Salzer,E., Zoghi,S., Kiss,M.G., Kage,F., Rashkova,C., Stahnke,S.,
            Haimel,M., Platzer,R., Caldera,M., Ardy,R.C., Hoeger,B., Block,J.,
            Medgyesi,D., Sin,C., Shahkarami,S., Kain,R., Ziaee,V., Hammerl,P.,
            Bock,C., Menche,J., Dupre,L., Huppa,J.B., Sixt,M., Lomakin,A.,
            Rottner,K., Binder,C.J., Stradal,T.E.B., Rezaei,N. and Boztug,K.
  TITLE     The cytoskeletal regulator HEM1 governs B cell development and
            prevents autoimmunity
  JOURNAL   Sci Immunol 5 (49) (2020)
   PUBMED   32646852
  REMARK    GeneRIF: The cytoskeletal regulator HEM1 governs B cell development
            and prevents autoimmunity.
REFERENCE   6  (bases 1 to 4717)
  AUTHORS   Thiam,H.R., Vargas,P., Carpi,N., Crespo,C.L., Raab,M., Terriac,E.,
            King,M.C., Jacobelli,J., Alberts,A.S., Stradal,T.,
            Lennon-Dumenil,A.M. and Piel,M.
  TITLE     Perinuclear Arp2/3-driven actin polymerization enables nuclear
            deformation to facilitate cell migration through complex
            environments
  JOURNAL   Nat Commun 7, 10997 (2016)
   PUBMED   26975831
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4717)
  AUTHORS   Chan,M.M., Wooden,J.M., Tsang,M., Gilligan,D.M., Hirenallur-S,D.K.,
            Finney,G.L., Rynes,E., Maccoss,M., Ramirez,J.A., Park,H. and
            Iritani,B.M.
  TITLE     Hematopoietic protein-1 regulates the actin membrane skeleton and
            membrane stability in murine erythrocytes
  JOURNAL   PLoS One 8 (2), e54902 (2013)
   PUBMED   23424621
  REMARK    GeneRIF: a novel, critical role for Hem-1 in the homeostasis of
            structural proteins required for formation and stability of the
            actin membrane skeleton in erythrocytes
REFERENCE   8  (bases 1 to 4717)
  AUTHORS   Abdallah,B.M., Ditzel,N., Mahmood,A., Isa,A., Traustadottir,G.A.,
            Schilling,A.F., Ruiz-Hidalgo,M.J., Laborda,J., Amling,M. and
            Kassem,M.
  TITLE     DLK1 is a novel regulator of bone mass that mediates estrogen
            deficiency-induced bone loss in mice
  JOURNAL   J Bone Miner Res 26 (7), 1457-1471 (2011)
   PUBMED   21308776
REFERENCE   9  (bases 1 to 4717)
  AUTHORS   Park,H., Staehling-Hampton,K., Appleby,M.W., Brunkow,M.E.,
            Habib,T., Zhang,Y., Ramsdell,F., Liggitt,H.D., Freie,B., Tsang,M.,
            Carlson,G., Friend,S., Frevert,C. and Iritani,B.M.
  TITLE     A point mutation in the murine Hem1 gene reveals an essential role
            for Hematopoietic protein 1 in lymphopoiesis and innate immunity
  JOURNAL   J Exp Med 205 (12), 2899-2913 (2008)
   PUBMED   19015308
  REMARK    GeneRIF: results demonstrate that Hem1 is essential for
            hematopoietic cell development, function, and homeostasis by
            controlling a distinct pathway leading to cytoskeletal
            reorganization
REFERENCE   10 (bases 1 to 4717)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC131721.4.
            
            On May 1, 2007 this sequence version replaced NM_153505.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083390.1, BC007186.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC131721.4         137038-137194
            158-268             AC131721.4         138184-138294
            269-361             AC131721.4         139053-139145
            362-418             AC131721.4         143398-143454
            419-561             AC131721.4         144752-144894
            562-652             AC131721.4         145715-145805
            653-790             AC131721.4         145893-146030
            791-839             AC131721.4         147579-147627
            840-996             AC131721.4         147719-147875
            997-1053            AC131721.4         154442-154498
            1054-1150           AC131721.4         154740-154836
            1151-1260           AC131721.4         155981-156090
            1261-1393           AC131721.4         156252-156384
            1394-1475           AC131721.4         156730-156811
            1476-1534           AC131721.4         157013-157071
            1535-1680           AC131721.4         157266-157411
            1681-1813           AC131721.4         158815-158947
            1814-1933           AC131721.4         159191-159310
            1934-2079           AC131721.4         161491-161636
            2080-2211           AC131721.4         161937-162068
            2212-2430           AC131721.4         165173-165391
            2431-2561           AC131721.4         166728-166858
            2562-2659           AC131721.4         169545-169642
            2660-2753           AC131721.4         169759-169852
            2754-2836           AC131721.4         170006-170088
            2837-2917           AC131721.4         170322-170402
            2918-3011           AC131721.4         173205-173298
            3012-3128           AC131721.4         174124-174240
            3129-3238           AC131721.4         174679-174788
            3239-3328           AC131721.4         176434-176523
            3329-4717           AC131721.4         180625-182013
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 58.96 cM"
     gene            1..4717
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="NCK associated protein 1 like"
                     /db_xref="GeneID:105855"
                     /db_xref="MGI:MGI:1926063"
     exon            1..157
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..3460
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="hematopoietic protein 1"
                     /codon_start=1
                     /product="nck-associated protein 1-like"
                     /protein_id="NP_705725.1"
                     /db_xref="CCDS:CCDS37236.1"
                     /db_xref="GeneID:105855"
                     /db_xref="MGI:MGI:1926063"
                     /translation="MSLTSAYQLKLAEKLTILNDRGQGVLIRMYNIKKTCSDSKSKPP
                     FLLEKSMESCLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFR
                     DHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNC
                     AHEMLHGHSDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQG
                     AEQWRSAQLLSLISSPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQ
                     CQKLWKLCLEGSLYITLIREDVLQVHKVTEDLFSSLKGYSKRVADIKESKEHAITNSG
                     QFHCQRRQFLRTAVKELETVLNDEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTK
                     TKTPEDYADSSIAELLFLLEEIRALVRRHIKVIQQYHLQYLARFDVLVLSDIIQNLSV
                     CPEEESVIMSSFVSILSSLNLKQVDNEEKFDFSGLRLDWFRLQAYTSVSKAPLHLHEN
                     PDLAKVMNLIIFHSQMLDSVEKMLVETSDLSTFCFHLRTFEKMFATTLEEPTMLRYTI
                     AFPLICAHFVHCVHEMCPEEYPHLKNHGLHHCNSFLEDLAKQTSNCVLEICAEQRNLN
                     EQLLPKHCATTISKAKNKKSMKQRQAPRKGEPERDKPGAESHRKNRSLVTNMDKLHLN
                     LTELALAMNHVHSFSVFEHTIFPSEYLSSHLEARLNRAIVTLAGYNATTQEILRPSEL
                     LAGVKAYISFIQSLAQFLGADASRIVRNALLQQTQPLDSSGEQTVTTLYTNWYLESLL
                     RQASSGAIVLSPAMQAFISLPRDGEQNFSAEEFSDISEMRALAEILGPYGMKFLSENL
                     MWHVTSQIVELKKLVVENMDILVQIRSNFSKPELMASLLPQLTGAENVLKRMTIIGVI
                     LSFRAMAQEGLQEVFSAHCPFLMGPIECLKEFVTPDTDIKVTLSVFELACAAGVSCDI
                     DPALVAAIANLKADNSSPEEEYKVACLLLIFLAVSLPLLATDPSSFFSIEKDGYNNNI
                     HCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLL
                     MRLVVEESPFLTLDMLESCFPYVLLRNAYREVSRAFYLNRLPASSH"
     exon            158..268
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            269..361
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            362..418
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            419..561
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..652
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            653..790
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            791..839
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            840..996
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1053
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1150
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1260
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1393
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1475
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1534
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1680
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1813
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1933
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2079
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2211
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2430
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2561
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2659
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2753
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2836
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..2917
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3011
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..3128
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3238
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3328
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3329..4717
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3652..3657
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="hexamer: GATAAA"
     polyA_site      3673
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="major polyA site"
     regulatory      3837..3842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="hexamer: ATTAAA"
     polyA_site      3859
                     /gene="Nckap1l"
                     /gene_synonym="4930568P13Rik; Hem1; Hemp1"
                     /note="major polyA site"
ORIGIN      
        1 tatttggggc atttgggtct tgttatccgg atggtcctct ctctcggtgg ccatcatgtc
       61 tttgacctct gcctatcagc ttaaattagc agagaagctc acgatcctga atgaccgagg
      121 acagggagtt ctcatccgca tgtacaacat caagaagacc tgttcagatt caaaatccaa
      181 gccacctttt ttactggaaa agtccatgga atcatgtctc aagtacatca acaagaagtt
      241 tcccaacata gatgtccgaa atagcacgca acatttagga ccagtgcatc gtgaaaaagc
      301 tgagataatt agattcctca ctaactatta ccagtcattt gtggatgtca tggaatttcg
      361 ggatcatgta tatgaacttc tcaacaccat tgatgcctgc cagtgtcatt ttgatattaa
      421 tctcaacttt gacttcactc ggagctacct ggacttaatt gtgacgtaca cctcagtcat
      481 tttactcctg tcacggattg aggaccgcag gatactcatt ggcatgtaca actgtgccca
      541 tgagatgctg catgggcaca gtgaccccag ttttgctcgc ctgggccaga tggtattaga
      601 gtatgaccat cctctgaaga agctgacaga agaatttgga cctcacacaa aggctgtgag
      661 tggagccctg ctctctctgc acttcctctt tgttcggagg aaccagggag ctgagcagtg
      721 gcgcagcgcc cagcttctaa gcctcatcag cagccccccg gccatgatta accctgctaa
      781 ttcagacaca atggcctgtg agtatctgtc cgtggaagtg atggagcgat ggatcataat
      841 tgggtttctt ctttgccatg ggtgcctcaa ctccaacagc cagtgccaga agctgtggaa
      901 gctgtgtctg gagggttccc tgtacatcac gctcatccgg gaagatgtgc tgcaagtgca
      961 caaggtcacc gaggacctgt tcagcagttt gaaagggtac agtaagcgag tggcagacat
     1021 caaggagagc aaggaacacg ccattacaaa cagtggccag tttcactgtc aacgccggca
     1081 gtttctgcgg acagctgtga aggagttgga gactgtgtta aatgatgaac caggcctgct
     1141 tggtcctaag gccctttttg ctttcatggc cctgtctttc attcgtgatg aggtcacttg
     1201 gctggttcgc catacagaaa atgtcaccaa gactaagaca cccgaagact atgctgactc
     1261 gagcattgca gagcttctgt tcctgttgga ggagatcagg gctctggtca gaagacacat
     1321 caaagttatt cagcagtacc acctccagta cctggccaga tttgatgtcc ttgtgcttag
     1381 tgacatcatt cagaatctgt ccgtgtgtcc agaggaggag tctgtcatta tgtcctcatt
     1441 tgttagtata ctgtcctctc tgaatctcaa gcaagttgat aatgaagaga aatttgactt
     1501 ttctggatta aggctggatt ggtttcgcct acaggcatat accagcgtgt cgaaggcacc
     1561 tttgcacctg catgagaacc ctgacctagc caaggtcatg aacctcatca tctttcactc
     1621 ccagatgttg gactcagtag agaaaatgct ggtggaaacc tctgaccttt ctactttctg
     1681 ctttcatctc cgtacctttg agaagatgtt tgctacgacc ctggaggaac caaccatgct
     1741 tcgctacacc attgccttcc ccctgatttg tgctcatttt gtccactgcg ttcatgaaat
     1801 gtgcccagag gagtaccctc acctgaagaa ccatggtctt caccactgca actccttcct
     1861 ggaagatctg gccaagcaga ccagcaactg tgtgcttgag atatgtgcgg aacagcggaa
     1921 cctgaatgaa cagcttctac ctaagcactg tgccaccacc atcagcaaag ccaagaataa
     1981 gaagtccatg aagcagaggc aggctcccag gaaaggagag cctgagaggg acaagccagg
     2041 agcagagagt catcggaaga accgcagcct tgtcactaac atggacaaac tacatctaaa
     2101 cttgactgaa ttggcactgg caatgaatca tgtgcacagt ttctctgtgt ttgaacacac
     2161 catcttccca tctgagtatc tcagcagtca cctggaggcc aggcttaaca gagccatcgt
     2221 gactttggct ggctacaatg ccacaaccca ggagatcctt aggccttctg agctgctggc
     2281 aggagtcaaa gcatacatca gtttcatcca gtcattggca cagtttttgg gtgcagatgc
     2341 ttccagaatc gttcgcaatg ccctcctgca gcagacacag ccactggact catctggcga
     2401 gcagactgtc accactctct acacaaactg gtacctagaa agtctcctta gacaggcgag
     2461 cagtggagcc attgtcctct ccccagccat gcaggccttc atcagccttc cccgagatgg
     2521 agagcagaac ttcagtgctg aggagttctc ggacatctct gagatgcgag ccttggctga
     2581 aattcttggt ccctatggca tgaagttcct gagtgaaaac ctgatgtggc atgtgacctc
     2641 tcagattgtg gagctgaaga agcttgtggt ggaaaacatg gatattctgg tccagatcag
     2701 atccaacttc agcaagccag aattaatggc atctctgctg ccccagctga caggagctga
     2761 aaatgtgctg aaacgaatga ccatcattgg ggtgattctc agttttaggg ccatggccca
     2821 agagggactt caggaggttt tttctgccca ctgccctttt ctcatgggcc ccattgagtg
     2881 cctgaaggag tttgtcaccc ctgacacaga cattaaggtc accctgagtg tctttgagct
     2941 ggcatgtgct gcaggggtga gctgtgacat tgacccagcc ttggtagctg ccattgccaa
     3001 tctgaaagct gataactcat cccctgaaga agagtataag gtggcatgcc tactcttgat
     3061 ctttcttgct gtttccctcc cactccttgc cactgacccg tcttccttct tcagcattga
     3121 gaaagatggc tacaacaaca acatccactg tttgaccaaa gccatcattc aggtgtctgc
     3181 tgccctcttc actctgtaca acaagaacat tgaaacgcac ctcaaagagt ttctggtggt
     3241 ggcctctgtc agcctcttgc agctgggcca ggagactgac aagctcaaaa ccagaaatcg
     3301 tgaatccatt tctctgctca tgcgcttggt ggtggaggag tcacccttct tgaccctgga
     3361 catgctggag tcctgtttcc cttacgttct gctacgcaat gcctatcggg aggtttcccg
     3421 ggccttctac ttgaaccgtc tgccagccag ttcccactga gggccctttg gaggtgccaa
     3481 gaaccatgct gcagtgaaag ttgcagccat ttcttcaaaa ggggagaaga gtgggaggga
     3541 gctcagggag agacaggtca ggccccacag ctgaaaggga taaggaggaa tatccagggc
     3601 tgaggaatca tggagaagtg gacaggctgg ggccatggag gacaatttat agataaagga
     3661 gtgtggaacc acatgtatga atttttaacc gtggggcggt ggtgaaaaac cagtgaccta
     3721 caagcatatt gtgtatcttc tggtgactcc agcttcagct ctgacaggca tgggcctctc
     3781 cgaccttcat cactattcta ggataatgtt ggcgggcaga gatgatcaat catcatatta
     3841 aaccataatg agcttataat cctcccgctg gagctgctat tgtctcctgc acattcatta
     3901 ggacgattat ctcctctcct tcttttccaa atgtgttcag caaatgtcca gcctgctcct
     3961 tctgcaacag agcagcacag gcacccactt ctctttcaag ggctcttggt ccatcttgcc
     4021 ttaactccat tccccactgt ggcaagggga aagtgtcctc ccgaggtatc atggccagcg
     4081 tactctccta tctactgtgg gtaaaaagtg tatcttccct gttcttaccg tgcctgcttt
     4141 tccttgtgtg tctccacagc ccagatcaga ggggcaatcc agatcacctg acagccaagt
     4201 aaaacagact cacatgggtt ttccctccta caccattaag gggggcggtt ttctacctcc
     4261 taatgggtgg gactaactta acccctaatc cccttgtcag tctgagtaca gctgttgctc
     4321 caggcaggga cttctcttgt agatcttaaa tttcacatac ctcattaggt cagagggcca
     4381 agagtaggga tccttgaggg aatacaggct gtttcaactt agcccttttc tgcactaatt
     4441 agaatttcaa gtgtcacaga gcctttagat agcactaact gggtttagta actttgtggc
     4501 agaaagcagt taaccccgtc cttcattcac acagtcattg ccagggaggc tggagtaaca
     4561 gatcagatgt gtcacaggtc ctgagtagtt ggaatgggaa aatataatta gccagttgat
     4621 taattagggt cgtgcccatg cttccattat gaaaaggaag caattgttgt tggctttcag
     4681 caaaaccatc cagctttcat taaaccaact tgatagc
//
