LOCUS       NM_016965               4403 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus NCK-associated protein 1 (Nckap1), transcript variant
            2, mRNA.
ACCESSION   NM_016965
VERSION     NM_016965.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Balekoglu,N., Michaud,J.F., Sauve,R., Ayinde,K.S., Lin,S., Liu,Y.,
            Kramer,D.A., Zhang,K., Steffen,A., Stradal,T., Angers,S., Chen,B.,
            Yam,P.T. and Charron,F.
  TITLE     The WAVE regulatory complex interacts with Boc and is required for
            Shh-mediated axon guidance
  JOURNAL   iScience 27 (12), 111333 (2024)
   PUBMED   39640588
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Swaminathan,K., Campbell,A., Papalazarou,V., Jaber-Hijazi,F.,
            Nixon,C., McGhee,E., Strathdee,D., Sansom,O.J. and Machesky,L.M.
  TITLE     The RAC1 Target NCKAP1 Plays a Crucial Role in the Progression of
            Braf;Pten-Driven Melanoma in Mice
  JOURNAL   J Invest Dermatol 141 (3), 628-637 (2021)
   PUBMED   32777214
  REMARK    GeneRIF: The RAC1 Target NCKAP1 Plays a Crucial Role in the
            Progression of Braf;Pten-Driven Melanoma in Mice.
REFERENCE   3  (bases 1 to 4403)
  AUTHORS   Chai,G., Webb,A., Li,C., Antaki,D., Lee,S., Breuss,M.W., Lang,N.,
            Stanley,V., Anzenberg,P., Yang,X., Marshall,T., Gaffney,P.,
            Wierenga,K.J., Chung,B.H., Tsang,M.H., Pais,L.S., Lovgren,A.K.,
            VanNoy,G.E., Rehm,H.L., Mirzaa,G., Leon,E., Diaz,J., Neumann,A.,
            Kalverda,A.P., Manfield,I.W., Parry,D.A., Logan,C.V., Johnson,C.A.,
            Bonthron,D.T., Valleley,E.M.A., Issa,M.Y., Abdel-Ghafar,S.F.,
            Abdel-Hamid,M.S., Jennings,P., Zaki,M.S., Sheridan,E. and
            Gleeson,J.G.
  TITLE     Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause
            Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly
  JOURNAL   Neuron 109 (2), 241-256 (2021)
   PUBMED   33220177
REFERENCE   4  (bases 1 to 4403)
  AUTHORS   Whitelaw,J.A., Swaminathan,K., Kage,F. and Machesky,L.M.
  TITLE     The WAVE Regulatory Complex Is Required to Balance Protrusion and
            Adhesion in Migration
  JOURNAL   Cells 9 (7), 1635 (2020)
   PUBMED   32646006
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4403)
  AUTHORS   Afzal,T.A., Luong,L.A., Chen,D., Zhang,C., Yang,F., Chen,Q., An,W.,
            Wilkes,E., Yashiro,K., Cutillas,P.R., Zhang,L. and Xiao,Q.
  TITLE     NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular
            Smooth Muscle Cell Migration, Proliferation, and Neointima
            Hyperplasia
  JOURNAL   J Am Heart Assoc 5 (12), e004629 (2016)
   PUBMED   27927633
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4403)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   7  (bases 1 to 4403)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   8  (bases 1 to 4403)
  AUTHORS   Steffen,A., Rottner,K., Ehinger,J., Innocenti,M., Scita,G.,
            Wehland,J. and Stradal,T.E.
  TITLE     Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia
            formation
  JOURNAL   EMBO J 23 (4), 749-759 (2004)
   PUBMED   14765121
  REMARK    GeneRIF: Nap1 is a constitutive and essential component of a WAVE2-
            and Abi-1-containing complex linking Rac to site-directed actin
            assembly.
REFERENCE   9  (bases 1 to 4403)
  AUTHORS   Baumgartner,S., Martin,D., Chiquet-Ehrismann,R., Sutton,J.,
            Desai,A., Huang,I., Kato,K. and Hromas,R.
  TITLE     The HEM proteins: a novel family of tissue-specific transmembrane
            proteins expressed from invertebrates through mammals with an
            essential function in oogenesis
  JOURNAL   J Mol Biol 251 (1), 41-49 (1995)
   PUBMED   7643388
REFERENCE   10 (bases 1 to 4403)
  AUTHORS   Kato,K.
  TITLE     A Collection of cDNA Clones with Specific Expression Patterns in
            Mouse Brain
  JOURNAL   Eur J Neurosci 2 (8), 704-711 (1990)
   PUBMED   12106288
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU055892.1, BC060197.1, AJ534525.1 and BP764873.1.
            
            On Mar 19, 2014 this sequence version replaced NM_016965.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' end compared to variant 1, resulting in an isoform
            (b) that is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060197.1, SRR1660817.106317.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                BU055892.1         83-103
            22-217              BC060197.1         1-196
            218-1977            AJ534525.1         1-1760
            1978-4378           BC060197.1         1956-4356
            4379-4403           BP764873.1         20-44               c
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 48.21 cM"
     gene            1..4403
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /note="NCK-associated protein 1"
                     /db_xref="GeneID:50884"
                     /db_xref="MGI:MGI:1355333"
     exon            1..325
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            326..436
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..529
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            530..586
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            587..729
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            730..820
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..958
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1007
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1164
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1221
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1318
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1425
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1558
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1640
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1699
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1845
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1978
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2098
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2238
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2370
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2589
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..2720
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2818
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2912
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..2995
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3076
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3170
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3287
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3288..3397
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3487
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3488..4387
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4358..4363
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /note="hexamer: AATAAA"
     polyA_site      4387
                     /gene="Nckap1"
                     /gene_synonym="H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1;
                     p125Nap1"
                     /note="major polyA site"
ORIGIN      
        1 gggggtgtac tggggcggag agcgccgcgg gtggaggcgg aagggcggcg ggcggaagcg
       61 agcgccgcgg ctgctgtgtc acggcgcggg tgggcgcacg gcatgggcgc gccgcgctga
      121 ggcggaggtc gcgggcgggc aggcgggcgg gcctgaggga ggcggcgagc cggggagtag
      181 cgcgcccggc ggcggtggcg gcggcggcgg ccgcaccatg tcgcgctccg tgctgcagcc
      241 cagtcagcag aagctggcgg agaagctcac catcctcaac gaccggggcg tcggcatgct
      301 cacgcgcctc tacaacatca agaaggcatg tggcgacccc aaggctaaac cctcctacct
      361 tattgacaaa aacctggaat ctgctgtgaa attcatagtc agaaaatttc ctgctgtaga
      421 aacgcgcaac aacaatcaac agctggcaca gctacagaaa gaaaaatcag agattctgaa
      481 aaatctggcg ttgtattact ttacatttgt agatgttatg gaatttaagg accatgtctg
      541 tgacttgctg aatactattg acgtttgcca agtcttcttt gatattactg tgaactttga
      601 tttaacaaag aactacttag acttgactgt aacctataca acattaatga tactgctgtc
      661 ccggattgaa gaacggaagg caatcattgg actatacaat tatgcacatg aaatgaccca
      721 tggagcaagt gaccgagaat atccacgcct tggtcagatg attgtggact atgaaaaccc
      781 tttaaaaaag atgatggaag aatttgtacc ccatagcaag tctctttcag atgcgctaat
      841 ttctcttcag atggtgtatc ctagacggaa cctttcagca gaccagtgga ggaatgccca
      901 gttgctgagt ctcatcagtg cacccagcac aatgcttaat cctgcgcagt cggacactat
      961 gccttgtgaa tatctctcct tggatgcaat ggagaaatgg atcatctttg gctttattct
     1021 gtgccatggg atgctaaata cggaggctac cgcactgaac ctttggaagc tagctcttca
     1081 gagtagctct tgcctctctc tctttcggga tgaagttttc cacattcaca aagctgcaga
     1141 agacttattt gtaaacattc ggggctacaa taaacgtatt aacgacataa gagaatgcaa
     1201 ggaggcggct gtatcccacg ccggctcaat gcacagagaa agacgcaagt ttttacggtc
     1261 tgcactaaaa gaactggcca ctgtcctctc tgaccagcct ggcctgcttg gtcccaaggc
     1321 actttttgtt ttcatggcgt tatcttttgc ccgtgatgaa attatctggc tgctccgtca
     1381 cgcggacaac atgccaaaga agagtgcaga tgactttata gataagcaca ttgctgagct
     1441 aatattttac atggaagagc ttagagcaca tgtcaggaag tatgggcctg tcatgcagag
     1501 gtactacgtg caatacctct ctggctttga tgctgttgtc ctcaatgaac ttgtgcagaa
     1561 cctgtctgtt tgccctgagg atgaatcgat catcatgtcc tcttttgtga acaccatgac
     1621 ctccctaagt gtgaagcaag ttgaagatgg agaagtgttt gatttcagag ggatgagatt
     1681 agattggttt aggttacagg cgtacactag cgtctccaaa gcttcactta gccttgcaga
     1741 tcacagagaa cttggaaaga tgatgaacac aatcatattc catacaaaaa tggtagattc
     1801 cttggttgaa atgttggtgg aaacatccga tctctccata ttttgttttt acagccgtgc
     1861 ttttgagaag atgtttcagc agtgcttgga gctgccctct cagtcaagat actccattgc
     1921 ctttcctctg ctctgcactc acttcatgag ctgcacacat gaactgtgtc ctgaagagcg
     1981 ccatcatata ggagatcgta gcctttccct gtgcaatatg ttcctggatg agatggccaa
     2041 acaagctcga aatctcatca ccgatatttg cacagaacag tgtactctta gtgaccagtt
     2101 attgccgaag cattgtgcca aaaccatcag tcaagcagtg aataagaagt caaaaaaaca
     2161 gacgggcaag aaaggggaac ctgaaaggga aaaaccgggt gtggagagca tgaggaaaaa
     2221 caggctggta gtgaccaacc ttgataagtt gcacactgca ctttcggagt tatgcttctc
     2281 cataaattat gttccaaaca tggcggtgtg ggagcacacc ttcacgccga gggagtattt
     2341 gacttctcat ctggaaatcc ggttcactaa atcaattgtt ggaatgacta tgtataatca
     2401 agctacacag gaaattgcaa agccatcaga gcttctaaca agcgtaagag catatatgac
     2461 tgtactccag tccatagaga actatgtgca gattgatatc accagagtat ttaataatgt
     2521 ccttcttcag caaacacaac acttagacag ccatggagaa ccaaccatca cgagtctgta
     2581 tacaaactgg tacttggaaa ctttattaag acaagtcagc aatggccata tagcttattt
     2641 tcctgcaatg aaagcatttg tgaatttacc cacagaaaat gaattaacat tcaatgcaga
     2701 ggaatattct gatatatcag aaatgagatc attatcagag ctcctaggcc catatgggat
     2761 gaagttcctg agtgagagcc ttatgtggca catttcatca caagtagctg aacttaagaa
     2821 acttgtggtg gagaatgttg atgttctaac acaaatgagg accagctttg acaaaccaga
     2881 ccagatggct gcactcttta aaagattatc atctgttgac agtgtcttga aaaggatgac
     2941 gataattggt gttattttat ccttccggtc attggcacaa gaagcattga gagatgtcct
     3001 gtcctaccac attcctttcc tcgtgagctc cattgaagat ttcaaggatc acattcctcg
     3061 ggaaactgat atgaaggttg caatgaatgt gtatgagctg tcctcggctg ctgggttacc
     3121 atgtgagatt gatcctgcct tagtcgtagc tctttcctca caaaaatcag agaacattag
     3181 tccagaggaa gaatacaaga tcgcttgtct cctgatggtc tttgtagcag tttccttgcc
     3241 tacactggcc agcaatgtaa tgtcacagta cagccctgca atagaaggac actgcaacaa
     3301 tatacattgt ttggccaaag ccatcaacca gattgctgca gctttgttta caattcacaa
     3361 aggaagtatt gaagaccgac ttaaggaatt cctggcgctt gcatcatcca gtctactgaa
     3421 aattggccaa gagacagata aaactacaac aagaaataga gaatctgttt atttgctact
     3481 ggatatgatt gtacaagaat caccattcct gacaatggat ctcttggaat cttgttttcc
     3541 ttatgtgttg ctgagaaatg cataccatgc tgtctacaaa caaagtgtta catcttctgc
     3601 atagagtact tactcaagac aagcacgagt ttctgttgcc ttgttttatc tgtaaactgt
     3661 ggaactattt taccttaaag cctgaaaaca gttttgtgga tgaaattttc ttcatgctgt
     3721 tgtattttct gatcattggc ttcctaatat ggttgtacta cagtacattt gattgattta
     3781 gttatacatt ctcaaaactc agtgaattct atgattttag agtatcattt gtttgaaata
     3841 ccattaacac tatgtttttg ataactgata atgaaaaaac ttgcttgttt atttctgaaa
     3901 aagaactgta tttagtgatt attttagata gtgatatgct agcccccatc tgtgtgtaaa
     3961 ttatttccta tagggagagt tctgatctgt acctacggtt ccttctgaga tggagactgg
     4021 atgtgcacat cggtgatgct tacgactaca tttctacttt atgttgaaca tttgccaaac
     4081 taaataccta acgcggttta accttccaca tggcagacaa cttgagcatc ggaagcaggt
     4141 tccgaggttc tcagacagca gcacatgctg ccgccgcctg tagaaagcct actgtagaaa
     4201 catctcatcc cgtgctgccc ggctggcatc tgcactgcct ttaccctgct gtataataaa
     4261 caaccagaca cttggcaact gttgatacag atggtagtcc cgaatcactt tttatatgtt
     4321 ttaacatttg aaattcaagt gtacctgcca taacataaat aaacactaga ctgtatcaca
     4381 ccttgaaaaa aaaaaaaaaa aaa
//
