LOCUS       NM_010879               2789 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus non-catalytic region of tyrosine kinase adaptor
            protein 2 (Nck2), mRNA.
ACCESSION   NM_010879 XM_904409 XM_918831
VERSION     NM_010879.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Golding,A.P., Ferrier,B., New,L.A., Lu,P., Martin,C.E., Shata,E.,
            Jones,R.A., Moorehead,R.A. and Jones,N.
  TITLE     Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary
            Gland Development
  JOURNAL   J Mammary Gland Biol Neoplasia 28 (1), 19 (2023)
   PUBMED   37479911
  REMARK    GeneRIF: Distinct Requirements for Adaptor Proteins NCK1 and NCK2
            in Mammary Gland Development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Bywaters,B.C., Pedraza,G., Trache,A. and Rivera,G.M.
  TITLE     Endothelial NCK2 promotes atherosclerosis progression in male but
            not female Nck1-null atheroprone mice
  JOURNAL   Front Cardiovasc Med 9, 955027 (2022)
   PUBMED   36035930
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2789)
  AUTHORS   Fernandez-Pisonero,I., Clavain,L., Robles-Valero,J.,
            Lorenzo-Martin,L.F., Caloto,R., Nieto,B., Garcia-Macias,C.,
            Oeste,C.L., Sanchez-Martin,M., Abad,A., Hortal,A., Caballero,D.,
            Gonzalez,M., Dosil,M., Alarcon,B. and Bustelo,X.R.
  TITLE     A hotspot mutation targeting the R-RAS2 GTPase acts as a potent
            oncogenic driver in a wide spectrum of tumors
  JOURNAL   Cell Rep 38 (11), 110522 (2022)
   PUBMED   35294890
REFERENCE   4  (bases 1 to 2789)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2789)
  AUTHORS   Alfaidi,M., Acosta,C.H., Wang,D., Traylor,J.G. and Orr,A.W.
  TITLE     Selective role of Nck1 in atherogenic inflammation and plaque
            formation
  JOURNAL   J Clin Invest 130 (8), 4331-4347 (2020)
   PUBMED   32427580
REFERENCE   6  (bases 1 to 2789)
  AUTHORS   Cowan,C.A. and Henkemeyer,M.
  TITLE     The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals
  JOURNAL   Nature 413 (6852), 174-179 (2001)
   PUBMED   11557983
  REMARK    GeneRIF: Transduces B-ephrin reverse signals.
REFERENCE   7  (bases 1 to 2789)
  AUTHORS   Lock,P., Casagranda,F. and Dunn,A.R.
  TITLE     Independent SH2-binding sites mediate interaction of Dok-related
            protein with RasGTPase-activating protein and Nck
  JOURNAL   J Biol Chem 274 (32), 22775-22784 (1999)
   PUBMED   10428862
REFERENCE   8  (bases 1 to 2789)
  AUTHORS   Braverman,L.E. and Quilliam,L.A.
  TITLE     Identification of Grb4/Nckbeta, a src homology 2 and 3
            domain-containing adapter protein having similar binding and
            biological properties to Nck
  JOURNAL   J Biol Chem 274 (9), 5542-5549 (1999)
   PUBMED   10026169
REFERENCE   9  (bases 1 to 2789)
  AUTHORS   Chen,M., She,H., Davis,E.M., Spicer,C.M., Kim,L., Ren,R., Le
            Beau,M.M. and Li,W.
  TITLE     Identification of Nck family genes, chromosomal localization,
            expression, and signaling specificity
  JOURNAL   J Biol Chem 273 (39), 25171-25178 (1998)
   PUBMED   9737977
REFERENCE   10 (bases 1 to 2789)
  AUTHORS   Margolis,B., Silvennoinen,O., Comoglio,F., Roonprapunt,C.,
            Skolnik,E., Ullrich,A. and Schlessinger,J.
  TITLE     High-efficiency expression/cloning of epidermal growth
            factor-receptor-binding proteins with Src homology 2 domains
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 8894-8898 (1992)
   PUBMED   1409582
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051665.1, AK014949.1 and BQ176012.1.
            
            On Jun 18, 2008 this sequence version replaced NM_010879.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051665.1, AK014949.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1482              AK051665.1         1-1482
            1483-2541           AK014949.1         1477-2535
            2542-2789           BQ176012.1         1-248               c
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 23.18 cM"
     gene            1..2789
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /note="non-catalytic region of tyrosine kinase adaptor
                     protein 2"
                     /db_xref="GeneID:17974"
                     /db_xref="MGI:MGI:1306821"
     exon            1..277
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            278..440
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            441..682
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             457..1599
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /note="NCK adaptor protein 2; SH2/SH3 adaptor protein
                     NCK-beta; growth factor receptor-bound protein 4"
                     /codon_start=1
                     /product="cytoplasmic protein NCK2"
                     /protein_id="NP_035009.3"
                     /db_xref="CCDS:CCDS14924.1"
                     /db_xref="GeneID:17974"
                     /db_xref="MGI:MGI:1306821"
                     /translation="MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNA
                     ANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKPSARDASPTPSTDAEYPAN
                     GSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSFNGQIGWF
                     PSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVTEEELSFEK
                     GETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSA
                     SGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFK
                     VQLVDSVYCIGQRRFHSMDELVEHYKKAPIFTSEHGEKLYLVRALQ"
     exon            683..1404
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..2775
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2753..2758
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /note="hexamer: AATAAA"
     polyA_site      2775
                     /gene="Nck2"
                     /gene_synonym="4833426I10Rik; Grb4; Nck-2; NCKbeta"
                     /note="major polyA site"
ORIGIN      
        1 aggcgggcgc tgcgcgggag ggtcgccgcc gcggatcgca ggccggtgcg cgcggccgag
       61 cgggcggcgg cggcgacggt ggtggtgagg agggctcggc ggcaggcagg ctcggggctc
      121 ccgggccggc ggggtccgcc gggtccaatt gcgtccgctt ggtggcggga ggagggagcg
      181 gtgcagacaa agagcggcat ctgggcgggc gcagcgcggc cgccgccccg ggactcgcgc
      241 tgctgccctc cggcctcggc gtgcactgtg cggcgagatc ccacgggttc ctctcctata
      301 gtgtcatccc agctggctta agagcttgga cgatcactat agctctgtca ccttcaaggt
      361 gtcctcagac agcagaaaac ctcatctttt tgaagagtgc tacaattttt ccagtcacct
      421 cttgtaactg cgtgccaaag aaggactcca tgaaagatga cagaagaagt cattgtcata
      481 gccaagtggg actacactgc ccagcaggac caggagctcg acatcaggaa gaacgagcgg
      541 ttgtggctgc tagatgactc caagacgtgg tggcgggtga ggaatgctgc caacaggaca
      601 ggctacgtgc cttccaacta tgtggagcgt aagaacagcc tgaagaaggg ctccctggtg
      661 aagaacctca aggacacact aggcctcgga aagacgcgca ggaagccaag cgctcgggat
      721 gcttccccaa cgccaagcac tgatgcggag tacccagcca atggcagcgg tgctgaccgc
      781 atctatgacc tcaacatccc agcctttgtc aagttcgcct acgtagcaga acgagaggat
      841 gagctgtccc tggtgaaggg ctcccgtgtc acagttatgg agaaatgcag tgatggctgg
      901 tggcgtggca gcttcaacgg gcagatcggc tggttcccct ccaactacgt tttggaggaa
      961 gcggacgaag ctgcggctga ggcccccagc ttcctgagcc tacgtcgggg cactgcactg
     1021 agcaatgggc agggtgcacg ggtgctgcac gtggtgcaga cgctgtaccc cttcagctcg
     1081 gtcactgagg aggagcttag tttcgagaag ggggagacca tggaggtgat tgagaagccg
     1141 gagaatgacc ctgaatggtg gaaatgcaaa aatgcccgag gccaagtggg cctggtcccc
     1201 aaaaactacg tggttgttct cagtgatggg cctgctctgc accccgctca caccccccag
     1261 atcagctaca ccgggccttc agccagcggg cgctttgctg gtcgggagtg gtactatggc
     1321 aacgtgacac ggcaccaggc cgagtgtgcg ctcaatgagc ggggcgtcga gggcgacttc
     1381 ctcattaggg acagcgagtc ctcgcccagt gacttctccg tgtctctcaa agcgtcaggg
     1441 agaaacaagc acttcaaggt gcagctggtg gacagcgtct actgcattgg gcagcggcgg
     1501 ttccacagca tggacgagct tgtggagcac tacaagaagg cccccatctt caccagcgag
     1561 cacggggaga agctctacct tgtccgagcc ctacagtgaa agcagccatt ggccccctca
     1621 tgccctgccc actgtgggcc tcgctgccac ctctgcctcc cagagcccag cacttctggc
     1681 cacctccacc catgtggctt ggatcacctc tgtggcccag tctgtccttc ttttcagccc
     1741 tgttggtcaa ccacggctac ctaggccctc acaaccatgc cccaggccac caccttctgg
     1801 tgatgagggt cagagggagc aaagctactc acttctttcc tttccagctg gaaacagcct
     1861 gtttgaatcc ttacacacac atacacagag agggaagggg ggtgcagaga gagagactga
     1921 ctaaaagact ggagctgctt cagagggtcg cgaggctgta tgtagtgaat atgtgacctt
     1981 ggcacacact ccgagagctg tgtccatggg tgtttggtgc ctctcgtcag catgctggtc
     2041 cagaccggca gcctgtgtcc tgggcctgct ttgctttgct ttctttcttt ctttttcttt
     2101 tttttttttt tcaattttat tttttaattt tattttattt tggctccagg cactgcagga
     2161 gtccaaaact gtgcggatag caatactgga aaccctgggg agatggtggt gagaaaactg
     2221 cccagtgcct ttgaggctgt acttgcaata aaagagggtt ttggtaagag ttggtctgca
     2281 aaagaggaag gtggcaacac agaaagaaag gtactctggt gatgtccccc aggtgtgcca
     2341 tccacatcgt gcttttgccc ttgggcttgt ttgaatttca caattatgta tttaattctc
     2401 aaagtaatat gtatctgtag ctggttgttg acactaataa catgattaag gaaaacagct
     2461 gatctcttgg gaaggggagc taccaatact ttatacatac actatatata tatatataat
     2521 atgtgtatat tatatatata ttatttgctt tacagggaaa tttttcaggg tttacaaaag
     2581 aatatgtgat tagtagtaac agaatgttta tgaagaaatt gcattttcat ttttctttac
     2641 atttgaactt ctttatagtt tgactataga gtcttgagat ggcacattcc tacaattgaa
     2701 gaaggggtct tgggatcccc taaacttgca tacccggttt tttgaatatt gtaataaaaa
     2761 aaagtattat gacaaaaaaa aaaaaaaaa
//
