LOCUS       NM_008677               1465 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript
            variant 1, mRNA.
ACCESSION   NM_008677
VERSION     NM_008677.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Xu,J., He,C., Cai,Y., Wang,X., Yan,J., Zhang,J., Zhang,F.,
            Urbonaviciute,V., Cheng,Y., Lu,S. and Holmdahl,R.
  TITLE     NCF4 regulates antigen presentation of cysteine peptides by
            intracellular oxidative response and restricts activation of
            autoreactive and arthritogenic T cells
  JOURNAL   Redox Biol 72, 103132 (2024)
   PUBMED   38547647
  REMARK    GeneRIF: NCF4 regulates antigen presentation of cysteine peptides
            by intracellular oxidative response and restricts activation of
            autoreactive and arthritogenic T cells.
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1465)
  AUTHORS   He,C., Luo,H., Coelho,A., Liu,M., Li,Q., Xu,J., Kramer,A.,
            Malin,S., Yuan,Z. and Holmdahl,R.
  TITLE     NCF4 dependent intracellular reactive oxygen species regulate
            plasma cell formation
  JOURNAL   Redox Biol 56, 102422 (2022)
   PUBMED   36095971
  REMARK    GeneRIF: NCF4 dependent intracellular reactive oxygen species
            regulate plasma cell formation.
            Erratum:[Redox Biol. 2022 Nov;57:102499. doi:
            10.1016/j.redox.2022.102499.. PMID: 36243565]
REFERENCE   4  (bases 1 to 1465)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1465)
  AUTHORS   van der Weyden,L., Speak,A.O., Swiatkowska,A., Clare,S.,
            Schejtman,A., Santilli,G., Arends,M.J. and Adams,D.J.
  TITLE     Pulmonary metastatic colonisation and granulomas in NOX2-deficient
            mice
  JOURNAL   J Pathol 246 (3), 300-310 (2018)
   PUBMED   30062795
REFERENCE   6  (bases 1 to 1465)
  AUTHORS   Ha,Y.J. and Lee,J.R.
  TITLE     Role of TNF receptor-associated factor 3 in the CD40 signaling by
            production of reactive oxygen species through association with
            p40phox, a cytosolic subunit of nicotinamide adenine dinucleotide
            phosphate oxidase
  JOURNAL   J Immunol 172 (1), 231-239 (2004)
   PUBMED   14688330
REFERENCE   7  (bases 1 to 1465)
  AUTHORS   Baehner,R.L., Millar-Groff,S. and Bringas,P.
  TITLE     Developmental expression of NADPH phagocytic oxidase components in
            mouse embryos
  JOURNAL   Pediatr Res 46 (2), 152-157 (1999)
   PUBMED   10447107
REFERENCE   8  (bases 1 to 1465)
  AUTHORS   Mizuki,K., Kadomatsu,K., Hata,K., Ito,T., Fan,Q.W., Kage,Y.,
            Fukumaki,Y., Sakaki,Y., Takeshige,K. and Sumimoto,H.
  TITLE     Functional modules and expression of mouse p40(phox) and p67(phox),
            SH3-domain-containing proteins involved in the phagocyte NADPH
            oxidase complex
  JOURNAL   Eur J Biochem 251 (3), 573-582 (1998)
   PUBMED   9490028
REFERENCE   9  (bases 1 to 1465)
  AUTHORS   Rajan,L., Kozak,C.A. and Dudley,J.P.
  TITLE     Chromosomal localization of acquired MMTV proviral integration
            sites in T-cell lymphomas
  JOURNAL   Mamm Genome 9 (1), 84-85 (1998)
   PUBMED   9434954
REFERENCE   10 (bases 1 to 1465)
  AUTHORS   Zhan,S., Kozak,C.A. and Chanock,S.J.
  TITLE     Cloning and chromosomal localization of ncf4, the mouse homologue
            of p40-phox
  JOURNAL   Immunogenetics 45 (3), 217-219 (1997)
   PUBMED   8995189
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589692.10.
            
            On Aug 25, 2022 this sequence version replaced NM_008677.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150510.1, BC025517.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-288               AL589692.10        70776-71063         c
            289-373             AL589692.10        65397-65481         c
            374-527             AL589692.10        64848-65001         c
            528-598             AL589692.10        62496-62566         c
            599-726             AL589692.10        60949-61076         c
            727-784             AL589692.10        60354-60411         c
            785-883             AL589692.10        59834-59932         c
            884-1014            AL589692.10        55109-55239         c
            1015-1080           AL589692.10        54826-54891         c
            1081-1465           AL589692.10        53283-53667         c
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.1 cM"
     gene            1..1465
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="neutrophil cytosolic factor 4"
                     /db_xref="GeneID:17972"
                     /db_xref="MGI:MGI:109186"
     exon            1..288
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..1276
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neutrophil cytosol factor 4; NCF-4; p40-phox; neutrophil
                     NADPH oxidase factor 4"
                     /codon_start=1
                     /product="neutrophil cytosol factor 4 isoform 1"
                     /protein_id="NP_032703.2"
                     /db_xref="CCDS:CCDS27610.1"
                     /db_xref="GeneID:17972"
                     /db_xref="MGI:MGI:109186"
                     /translation="MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIE
                     VKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIA
                     ETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGV
                     SPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGI
                     FPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRR
                     EFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSV
                     YNTVP"
     exon            289..373
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            374..527
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            528..598
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            599..726
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            727..784
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            785..883
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1014
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1080
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1465
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1441..1446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="hexamer: AATAAA"
     polyA_site      1465
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="major polyA site"
ORIGIN      
        1 acttcctcaa ctgctgtgca gctgggaagt ggctgagaag acgtgtgagg gccaggcagc
       61 tcaaagcctg aaggaggtac ttcaaggagg gtccagagaa gctgggcagg gcgggacctc
      121 ctcaaaggct gcccctgggg acaccccaag cttcaggaag ctctcagatg cacataggag
      181 gaagtgagag gtgaactcgg cctggatctg gttgggcaag gcccccatcc atctgctttt
      241 ctgactaccc acagccatgg ccctggccca gcagctgcga tcagagagcg actttgagca
      301 gcttccagac gatgtggccg tctcagccaa catcgctgac atcgaggaga agaggggctt
      361 caccagccac tttgtttttg tcatcgaggt caaaacaaaa ggagggtcca agtatctcat
      421 ctaccgccgc tatcgccagt tctacgccct gcagagcaag ctggaggagc ggtttgggcc
      481 agagagcaag aacagccctt tcacctgcag cctgcccaca ttgccagcca aagtctacat
      541 gggcgcaaaa caagagatcg ctgagactcg gatcccggcc ctcaatgcct acatgaagaa
      601 cctcctgagc ctgccggtct gcgtgctgat ggaccccgac gtcaggatct tcttctatca
      661 gtctgcatac gatgctgagc aggtgcccca ggcactccgc aggctccgac cgcgcacgcg
      721 caaaatcaag ggtgtgtctc cacaaggggc catcatggat cgcatggaag cgccaagagc
      781 agaggccttg tttgacttca ctgggaacag caaattggag ctaagtttca aagctggaga
      841 tgtgatcttc cttctcagta agatcaacaa agactggctg gagggcacct cccagggagc
      901 cacaggcatc ttcccagggt ccttcgtgaa gatccttaag gactttcccg aggacgagga
      961 caccaccaac tggctacgat gctacttcta tgaagacaca ggcaaaacca tcaaggacat
     1021 tgcggtggag gaggacctca gcagcacgcc cctgttcaaa gacctgctag cgctcatgag
     1081 gcgtgagttc cagagagaag acattgccct tagctaccag gatgctgagg gggacttggt
     1141 taggctgctg tcagatgagg atgtgggact catggtgaag caagcccgag gcctcccttc
     1201 ccagaagcgc ctcttcccct ggaagctgca tgtcacacag aaggacaact acagtgtcta
     1261 caacactgtc ccctgagtca cagggaccct gtggcaagga cggggcgctc agcaaagggc
     1321 ctttaccttc caagaacacg gagaccttca gggagactga tgtaagatgc aaacctcctg
     1381 gcccagagtg cctactggac aggattctat taagtttttg acactctacc ttttggattc
     1441 aataaaccat tctgtctcta agggc
//
