LOCUS       NM_001411422            1287 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript
            variant 3, mRNA.
ACCESSION   NM_001411422 XM_006520564
VERSION     NM_001411422.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Xu,J., He,C., Cai,Y., Wang,X., Yan,J., Zhang,J., Zhang,F.,
            Urbonaviciute,V., Cheng,Y., Lu,S. and Holmdahl,R.
  TITLE     NCF4 regulates antigen presentation of cysteine peptides by
            intracellular oxidative response and restricts activation of
            autoreactive and arthritogenic T cells
  JOURNAL   Redox Biol 72, 103132 (2024)
   PUBMED   38547647
  REMARK    GeneRIF: NCF4 regulates antigen presentation of cysteine peptides
            by intracellular oxidative response and restricts activation of
            autoreactive and arthritogenic T cells.
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1287)
  AUTHORS   He,C., Luo,H., Coelho,A., Liu,M., Li,Q., Xu,J., Kramer,A.,
            Malin,S., Yuan,Z. and Holmdahl,R.
  TITLE     NCF4 dependent intracellular reactive oxygen species regulate
            plasma cell formation
  JOURNAL   Redox Biol 56, 102422 (2022)
   PUBMED   36095971
  REMARK    GeneRIF: NCF4 dependent intracellular reactive oxygen species
            regulate plasma cell formation.
            Erratum:[Redox Biol. 2022 Nov;57:102499. doi:
            10.1016/j.redox.2022.102499.. PMID: 36243565]
REFERENCE   4  (bases 1 to 1287)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1287)
  AUTHORS   van der Weyden,L., Speak,A.O., Swiatkowska,A., Clare,S.,
            Schejtman,A., Santilli,G., Arends,M.J. and Adams,D.J.
  TITLE     Pulmonary metastatic colonisation and granulomas in NOX2-deficient
            mice
  JOURNAL   J Pathol 246 (3), 300-310 (2018)
   PUBMED   30062795
REFERENCE   6  (bases 1 to 1287)
  AUTHORS   Ha,Y.J. and Lee,J.R.
  TITLE     Role of TNF receptor-associated factor 3 in the CD40 signaling by
            production of reactive oxygen species through association with
            p40phox, a cytosolic subunit of nicotinamide adenine dinucleotide
            phosphate oxidase
  JOURNAL   J Immunol 172 (1), 231-239 (2004)
   PUBMED   14688330
REFERENCE   7  (bases 1 to 1287)
  AUTHORS   Baehner,R.L., Millar-Groff,S. and Bringas,P.
  TITLE     Developmental expression of NADPH phagocytic oxidase components in
            mouse embryos
  JOURNAL   Pediatr Res 46 (2), 152-157 (1999)
   PUBMED   10447107
REFERENCE   8  (bases 1 to 1287)
  AUTHORS   Mizuki,K., Kadomatsu,K., Hata,K., Ito,T., Fan,Q.W., Kage,Y.,
            Fukumaki,Y., Sakaki,Y., Takeshige,K. and Sumimoto,H.
  TITLE     Functional modules and expression of mouse p40(phox) and p67(phox),
            SH3-domain-containing proteins involved in the phagocyte NADPH
            oxidase complex
  JOURNAL   Eur J Biochem 251 (3), 573-582 (1998)
   PUBMED   9490028
REFERENCE   9  (bases 1 to 1287)
  AUTHORS   Rajan,L., Kozak,C.A. and Dudley,J.P.
  TITLE     Chromosomal localization of acquired MMTV proviral integration
            sites in T-cell lymphomas
  JOURNAL   Mamm Genome 9 (1), 84-85 (1998)
   PUBMED   9434954
REFERENCE   10 (bases 1 to 1287)
  AUTHORS   Zhan,S., Kozak,C.A. and Chanock,S.J.
  TITLE     Cloning and chromosomal localization of ncf4, the mouse homologue
            of p40-phox
  JOURNAL   Immunogenetics 45 (3), 217-219 (1997)
   PUBMED   8995189
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589692.10.
            
            On Aug 25, 2022 this sequence version replaced XM_006520564.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422598.725023.1,
                                           SRR13422586.366227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AL589692.10        70988-71063         c
            77-110              AL589692.10        70776-70809         c
            111-195             AL589692.10        65397-65481         c
            196-349             AL589692.10        64848-65001         c
            350-420             AL589692.10        62496-62566         c
            421-548             AL589692.10        60949-61076         c
            549-606             AL589692.10        60354-60411         c
            607-705             AL589692.10        59834-59932         c
            706-836             AL589692.10        55109-55239         c
            837-902             AL589692.10        54826-54891         c
            903-1287            AL589692.10        53283-53667         c
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.1 cM"
     gene            1..1287
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="neutrophil cytosolic factor 4"
                     /db_xref="GeneID:17972"
                     /db_xref="MGI:MGI:109186"
     exon            1..76
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            77..110
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..1098
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="isoform 1 is encoded by transcript variant 3;
                     neutrophil cytosol factor 4; NCF-4; p40-phox; neutrophil
                     NADPH oxidase factor 4"
                     /codon_start=1
                     /product="neutrophil cytosol factor 4 isoform 1"
                     /protein_id="NP_001398351.1"
                     /db_xref="GeneID:17972"
                     /db_xref="MGI:MGI:109186"
                     /translation="MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIE
                     VKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIA
                     ETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGV
                     SPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGI
                     FPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRR
                     EFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSV
                     YNTVP"
     exon            111..195
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            196..349
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            350..420
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            421..548
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            549..606
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            607..705
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            706..836
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            837..902
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1287
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1263..1268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="hexamer: AATAAA"
     polyA_site      1287
                     /gene="Ncf4"
                     /gene_synonym="p40phox"
                     /note="major polyA site"
ORIGIN      
        1 acttcctcaa ctgctgtgca gctgggaagt ggctgagaag acgtgtgagg gccaggcagc
       61 tcaaagcctg aaggagccat ggccctggcc cagcagctgc gatcagagag cgactttgag
      121 cagcttccag acgatgtggc cgtctcagcc aacatcgctg acatcgagga gaagaggggc
      181 ttcaccagcc actttgtttt tgtcatcgag gtcaaaacaa aaggagggtc caagtatctc
      241 atctaccgcc gctatcgcca gttctacgcc ctgcagagca agctggagga gcggtttggg
      301 ccagagagca agaacagccc tttcacctgc agcctgccca cattgccagc caaagtctac
      361 atgggcgcaa aacaagagat cgctgagact cggatcccgg ccctcaatgc ctacatgaag
      421 aacctcctga gcctgccggt ctgcgtgctg atggaccccg acgtcaggat cttcttctat
      481 cagtctgcat acgatgctga gcaggtgccc caggcactcc gcaggctccg accgcgcacg
      541 cgcaaaatca agggtgtgtc tccacaaggg gccatcatgg atcgcatgga agcgccaaga
      601 gcagaggcct tgtttgactt cactgggaac agcaaattgg agctaagttt caaagctgga
      661 gatgtgatct tccttctcag taagatcaac aaagactggc tggagggcac ctcccaggga
      721 gccacaggca tcttcccagg gtccttcgtg aagatcctta aggactttcc cgaggacgag
      781 gacaccacca actggctacg atgctacttc tatgaagaca caggcaaaac catcaaggac
      841 attgcggtgg aggaggacct cagcagcacg cccctgttca aagacctgct agcgctcatg
      901 aggcgtgagt tccagagaga agacattgcc cttagctacc aggatgctga gggggacttg
      961 gttaggctgc tgtcagatga ggatgtggga ctcatggtga agcaagcccg aggcctccct
     1021 tcccagaagc gcctcttccc ctggaagctg catgtcacac agaaggacaa ctacagtgtc
     1081 tacaacactg tcccctgagt cacagggacc ctgtggcaag gacggggcgc tcagcaaagg
     1141 gcctttacct tccaagaaca cggagacctt cagggagact gatgtaagat gcaaacctcc
     1201 tggcccagag tgcctactgg acaggattct attaagtttt tgacactcta ccttttggat
     1261 tcaataaacc attctgtctc taagggc
//
