LOCUS       NM_178772               4441 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus neutral cholesterol ester hydrolase 1 (Nceh1), mRNA.
ACCESSION   NM_178772
VERSION     NM_178772.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Sun,H.J., Ni,Z.R., Liu,Y., Fu,X., Liu,S.Y., Hu,J.Y., Sun,Q.Y.,
            Li,Y.C., Hou,X.H., Zhang,J.R., Zhu,X.X. and Lu,Q.B.
  TITLE     Deficiency of neutral cholesterol ester hydrolase 1 (NCEH1) impairs
            endothelial function in diet-induced diabetic mice
  JOURNAL   Cardiovasc Diabetol 23 (1), 138 (2024)
   PUBMED   38664801
  REMARK    GeneRIF: Deficiency of neutral cholesterol ester hydrolase 1
            (NCEH1) impairs endothelial function in diet-induced diabetic mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Rong,Y., Ji,S.Y., Zhu,Y.Z., Wu,Y.W., Shen,L. and Fan,H.Y.
  TITLE     ZAR1 and ZAR2 are required for oocyte meiotic maturation by
            regulating the maternal transcriptome and mRNA translational
            activation
  JOURNAL   Nucleic Acids Res 47 (21), 11387-11402 (2019)
   PUBMED   31598710
REFERENCE   3  (bases 1 to 4441)
  AUTHORS   Yamazaki,H., Takahashi,M., Wakabayashi,T., Sakai,K., Yamamuro,D.,
            Takei,A., Takei,S., Nagashima,S., Yagyu,H., Sekiya,M., Ebihara,K.
            and Ishibashi,S.
  TITLE     Loss of ACAT1 Attenuates Atherosclerosis Aggravated by Loss of
            NCEH1 in Bone Marrow-Derived Cells
  JOURNAL   J Atheroscler Thromb 26 (3), 246-259 (2019)
   PUBMED   30282838
REFERENCE   4  (bases 1 to 4441)
  AUTHORS   Sekiya,M., Yamamuro,D., Ohshiro,T., Honda,A., Takahashi,M.,
            Kumagai,M., Sakai,K., Nagashima,S., Tomoda,H., Igarashi,M.,
            Okazaki,H., Yagyu,H., Osuga,J. and Ishibashi,S.
  TITLE     Absence of Nceh1 augments 25-hydroxycholesterol-induced ER stress
            and apoptosis in macrophages
  JOURNAL   J Lipid Res 55 (10), 2082-2092 (2014)
   PUBMED   24891333
  REMARK    GeneRIF: reducing the cholesteryl ester content of foam cells,
            Nceh1 may protect against the pro-apoptotic effect of oxysterols
            and modulate the development of atherosclerosis.
REFERENCE   5  (bases 1 to 4441)
  AUTHORS   Sakai,K., Igarashi,M., Yamamuro,D., Ohshiro,T., Nagashima,S.,
            Takahashi,M., Enkhtuvshin,B., Sekiya,M., Okazaki,H., Osuga,J. and
            Ishibashi,S.
  TITLE     Critical role of neutral cholesteryl ester hydrolase 1 in
            cholesteryl ester hydrolysis in murine macrophages
  JOURNAL   J Lipid Res 55 (10), 2033-2040 (2014)
   PUBMED   24868095
  REMARK    GeneRIF: These results suggest that Nceh1 plays a dominant role
            over Lipe in the hydrolysis of CE and subsequent cholesterol efflux
            in MPMs
REFERENCE   6  (bases 1 to 4441)
  AUTHORS   Igarashi,M., Osuga,J., Isshiki,M., Sekiya,M., Okazaki,H.,
            Takase,S., Takanashi,M., Ohta,K., Kumagai,M., Nishi,M., Fujita,T.,
            Nagai,R., Kadowaki,T. and Ishibashi,S.
  TITLE     Targeting of neutral cholesterol ester hydrolase to the endoplasmic
            reticulum via its N-terminal sequence
  JOURNAL   J Lipid Res 51 (2), 274-285 (2010)
   PUBMED   19592704
  REMARK    GeneRIF: NCEH is targeted to the ER of macrophages, where it
            hydrolyzes CE to deliver cholesterol for efflux out of the cells.
REFERENCE   7  (bases 1 to 4441)
  AUTHORS   Okazaki,H., Igarashi,M., Nishi,M., Sekiya,M., Tajima,M., Takase,S.,
            Takanashi,M., Ohta,K., Tamura,Y., Okazaki,S., Yahagi,N., Ohashi,K.,
            Amemiya-Kudo,M., Nakagawa,Y., Nagai,R., Kadowaki,T., Osuga,J. and
            Ishibashi,S.
  TITLE     Identification of neutral cholesterol ester hydrolase, a key enzyme
            removing cholesterol from macrophages
  JOURNAL   J Biol Chem 283 (48), 33357-33364 (2008)
   PUBMED   18782767
  REMARK    GeneRIF: NCEH is responsible for a major part of nCEH activity in
            macrophages and may be a potential therapeutic target for the
            prevention of atherosclerosis.
REFERENCE   8  (bases 1 to 4441)
  AUTHORS   Nomura,D.K., Leung,D., Chiang,K.P., Quistad,G.B., Cravatt,B.F. and
            Casida,J.E.
  TITLE     A brain detoxifying enzyme for organophosphorus nerve poisons
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 6195-6200 (2005)
   PUBMED   15840715
REFERENCE   9  (bases 1 to 4441)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   10 (bases 1 to 4441)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121099.8 and AC157919.5.
            
            On Jan 23, 2023 this sequence version replaced NM_178772.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045363.1, BC082569.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-212               AC121099.8         32908-33119         c
            213-441             AC157919.5         69293-69521
            442-511             AC157919.5         72698-72767
            512-683             AC157919.5         86157-86328
            684-4441            AC157919.5         87826-91583
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 10.82 cM"
     gene            1..4441
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /note="neutral cholesterol ester hydrolase 1"
                     /db_xref="GeneID:320024"
                     /db_xref="MGI:MGI:2443191"
     exon            1..212
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /inference="alignment:Splign:2.1.0"
     exon            213..441
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /inference="alignment:Splign:2.1.0"
     exon            442..511
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /inference="alignment:Splign:2.1.0"
     exon            512..683
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /inference="alignment:Splign:2.1.0"
     exon            684..4441
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4420..4425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /note="hexamer: AATAAA"
     polyA_site      4441
                     /gene="Nceh1"
                     /gene_synonym="Aadacl1; B230106I24Rik; CPO-BP; Nceh"
                     /note="major polyA site"
ORIGIN      
        1 agaaacttgc tggtcttctc cgggtagcat cgcaccgtac cgcggtcagc tccggacagc
       61 ccggcctggg gacaatgagg tcgtcatgcg tcctactggc cgctctgctg gctctggctg
      121 cctactacgt gtacatccca ctgcccagcg cggtgtccga cccctggaaa ctgatgctgc
      181 tggacgccac tttccgcggc gcgcagcaag tgagtaacct gatacattcc ctgggactga
      241 accaccatct gatcgcgctg aatttcatca tcacctcttt tggcaagcaa agcgcaaggt
      301 cttctccgaa agtgaaggtg acagacaccg acttcgatgg ggtagaagtc cgagtatttg
      361 aaggttctcc gaaaccagaa gagccgctgc gacgcagcgt catctatatc cacggaggag
      421 gctgggccct ggctagtgca aagatcagct actatgacca gctgtgcaca acaatggctg
      481 aggagctgaa cgccgtcatc gtttctattg aatacaggct agtcccacag gtctattttc
      541 cggagcaaat ccatgatgtc atccgtgcca ctaaatattt cctgcagcca gaagtcttag
      601 acaagtacaa ggtcgaccct ggaagagtcg gtatttctgg agacagtgct ggtgggaatc
      661 tggccgcggc cctgggacaa cagtttacct acgttgccag cctgaagaat aagctcaaac
      721 tgcaggcttt ggtctaccca gtcctgcagg ctttggactt caacacaccc tcttaccagc
      781 aaagcatgaa cactccgatc ctgccgcgtc atgtcatggt taggtactgg ttagactact
      841 tcaagggcaa ctacgacttt gtagaggcca tgattgtgaa caatcacact tcacttgatg
      901 tagaaagggc tgcagccctc agggcccgtc tcgactggac atccctgttg ccctcgtcca
      961 tcaaaaagaa ctacaagcct atcatgcaga ccacagggaa cgccaggatc gtccaggaga
     1021 tccctcaact gctggacgct gctgcttctc cgctcatcgc agagcaggaa gtgctagagg
     1081 ccctcccgaa gacctacatc cttacctgcg agcacgacgt cctgcgggac gatgggatca
     1141 tgtacgccaa gcgcttggag agtgcgggtg tgaacgtgac cttggaccac tttgaggacg
     1201 gcttccatgg ttgtatgatt tttacaagct ggccgaccaa cttctccgtg ggaatccgga
     1261 ctaggaatag ttacatcaaa tggctggatc aaaacctgtg acggaggaaa ctcctcagac
     1321 gcatgaagct cttgacctcc gtttgctttg gaaaaaacaa aacaaaacaa aacaaaacaa
     1381 aaaacaaaaa cactgcgcat ttcaatcatc tgcacctttc cctcgttttg cattagtact
     1441 gaaatctgta ccctacagac cttataataa tttgcaaagt gactctgact aacgcggtta
     1501 agaacatctg aacttggttt cccaaatgac tcagtttttg ctttcttttc tgaagcccga
     1561 gtgctaggta ggaatggcca cagtttgaag agctagaaat agccttaggt cgggcttctg
     1621 ttatgtgctt tccagaagca gtttttacaa ctgtaggtaa ggttgtaaat tctgaggaac
     1681 aacaacacca ccaccacaac catgagcctt tcttcagttt gctcgggttg acgcgggctg
     1741 cagagcagcg tctagtggac attccgaggc cagctcttgg gactgtgtag cctctgcctc
     1801 ccaggatgtt gtctttcatg tgggaatttt gacaggattt ctagcaagac agatttcttc
     1861 tcgaaagctt ttcttcctca ggagtatgaa ttttctgagc tagctgctgt aaattcagtg
     1921 aggctaaagg gatacttgaa tgttattttt tttctcctaa gaaaatggga ggctacatgc
     1981 actggatatg tccttgggaa aatctttggg gaacaaactt atttaactgt agagtaagtt
     2041 tcctattatg aaataagtaa aggtagaaaa ataatggtct gttgttactt aagaatactc
     2101 ctggcataag ttactagaag tggtgtgtat atattcgggg gagtaaggaa ataagttatg
     2161 tttaataata gcttaagatg accagcaatg cctgtagaag gctttgtccg tttttggcag
     2221 aaggcctagg gcatcgaaaa ctcagccttt tcactcgtag ctgaaaatgg gactattttg
     2281 gtatagcagt tgtttttttt taagttattt aatgttaata tatatatata gctaaacagg
     2341 gtttttctga aaaacattat aaaaaattta agataaaagg tattttaaaa agaaaactac
     2401 cataaagtta tatccacatg taattttttt gaggagatcg ataagagtag taggcaccac
     2461 atgtaagtca gggtcaaata cttggatatt tatctgcata tgcaaattaa aataattaga
     2521 aactttctac ctagtacgtc atgttgtact aggtacatga tgctggtcag ccatccttaa
     2581 tccaaaattt tgagtcaagt cttataagtg gaaaattcca gtgaacattt attttgtata
     2641 taaaatttta aacctattgt gtgaagctac cctcaggcta tgtagatcag atgtataaaa
     2701 agcacaaatg aatatctcaa ttacatttgg gttccattct caagatattt tattatgtat
     2761 attcaattca aatattctaa tgtctagaaa aatgaaaacc ctggatattc tggtcctgag
     2821 ttgttcagat gagggagagc caacctatag tttaacagct taggtaagac tcactctgcc
     2881 catgctggcc aggggaaccc tcaggggact cctctgggtg aagggcagat gctagcactg
     2941 cctgttctta gttctccttc cattcctgtc ctgaagttct aactgccgag ccttaccttc
     3001 atagaaggtc ttcactgcca gagtgggcat cttgcatagt aagggattcc agattagata
     3061 tgagttgagc accctgcctt tcttcctatc accccgcttc tgctgatagt ccgtgctaca
     3121 ttaagggact gagcagagaa cactcagtca agtcctgatc catttcgtca tgaagagagc
     3181 aggacctttc cattctatga agggtgcgtg tttctggttt ccagcagcag gataacaagt
     3241 gggaatttac tcactgactt ctcatttatc ataaaagctg cagcaccttt atgtacagaa
     3301 caatgtcaca gtgtgtgtgt gtgtgtgtgt gtgagcgagc atgtgctgtc tgcatatgga
     3361 aataactaca gcacaggcta ccttgctgat gtgtccatgt cactggtctc ccttttagct
     3421 aaaatctgag agtagaataa cctgatcttt gaggcctctt ccaatgttag tcttttctaa
     3481 ggtaattttt agtaggtcat aattcagtat atactagcct actcatctct gaagactgac
     3541 tttgctcgta attttttttt aattggccac atagcattaa tgcctccaca gggttcctgt
     3601 gtttcacatt tacgatataa attgataccc atcttcagct gaggttgtta tggggggctg
     3661 gaatcacgtg ttttttaaac ctgatatttt cattcctaat tgagtgacag ccatctttta
     3721 aggtgttggg catggaaccc caggccttac ctgcttggca agtgctttac ccccgaacta
     3781 cacctgaagc tccattcctt gtcccttctg caccatttaa aggtgggaac tatattttga
     3841 ctttatctac ctctgagaaa gaggaaaatg taattattca cagagtttct caaaatggca
     3901 tctttcctcc caaactggga gccaaattct gcagaaatca tggacagtaa atggtgacag
     3961 atgtctgaag gggtctcaaa gatgcaaggg aacatggggt caaaggggcc acccaccaca
     4021 tctgatgata tgttagtttt gttgttcttt ggggttttat tcttccaggt atgttatgca
     4081 ctacagcctg atcttttcag tctaagacag catggcttca ctttcctgtg ttatgagaag
     4141 gtcacacttt ggaatactga aggacaatcc tgaagtgctg cagcgcatag tgaatgcctc
     4201 ttggtgtttt cacgttcgtc tgtgaggact catagactta cagaatgatg ccgaatggaa
     4261 tgttttgtgc atgacctttt aacctttggc tattgccaat tccccactgc aaaggggaag
     4321 gcagatttta tgaagagttt taggaataaa tacattttat agaatggaaa cccagtatgg
     4381 tactggggaa gtttattcat gcttgcaatc ttgtttggaa ataaaatggg tccgtctgga
     4441 a
//
