LOCUS       NM_026554               2054 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nuclear cap binding protein subunit 2 (Ncbp2), mRNA.
ACCESSION   NM_026554
VERSION     NM_026554.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Rutkowski,T.P., Purcell,R.H., Pollak,R.M., Grewenow,S.M.,
            Gafford,G.M., Malone,T., Khan,U.A., Schroeder,J.P., Epstein,M.P.,
            Bassell,G.J., Warren,S.T., Weinshenker,D., Caspary,T. and
            Mulle,J.G.
  TITLE     Behavioral changes and growth deficits in a CRISPR engineered mouse
            model of the schizophrenia-associated 3q29 deletion
  JOURNAL   Mol Psychiatry 26 (3), 772-783 (2021)
   PUBMED   30976085
REFERENCE   3  (bases 1 to 2054)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   4  (bases 1 to 2054)
  AUTHORS   Gebhardt,A., Habjan,M., Benda,C., Meiler,A., Haas,D.A., Hein,M.Y.,
            Mann,A., Mann,M., Habermann,B. and Pichlmair,A.
  TITLE     mRNA export through an additional cap-binding complex consisting of
            NCBP1 and NCBP3
  JOURNAL   Nat Commun 6, 8192 (2015)
   PUBMED   26382858
  REMARK    GeneRIF: NCBP2 is not required for cell viability and poly(A) RNA
            export.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2054)
  AUTHORS   Pabis,M., Neufeld,N., Steiner,M.C., Bojic,T., Shav-Tal,Y. and
            Neugebauer,K.M.
  TITLE     The nuclear cap-binding complex interacts with the U4/U6.U5
            tri-snRNP and promotes spliceosome assembly in mammalian cells
  JOURNAL   RNA 19 (8), 1054-1063 (2013)
   PUBMED   23793891
REFERENCE   6  (bases 1 to 2054)
  AUTHORS   Hosoda,N., Kim,Y.K., Lejeune,F. and Maquat,L.E.
  TITLE     CBP80 promotes interaction of Upf1 with Upf2 during
            nonsense-mediated mRNA decay in mammalian cells
  JOURNAL   Nat Struct Mol Biol 12 (10), 893-901 (2005)
   PUBMED   16186820
REFERENCE   7  (bases 1 to 2054)
  AUTHORS   Shu,H., Chen,S., Bi,Q., Mumby,M. and Brekken,D.L.
  TITLE     Identification of phosphoproteins and their phosphorylation sites
            in the WEHI-231 B lymphoma cell line
  JOURNAL   Mol Cell Proteomics 3 (3), 279-286 (2004)
   PUBMED   14729942
REFERENCE   8  (bases 1 to 2054)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2054)
  AUTHORS   Ohno,M., Segref,A., Bachi,A., Wilm,M. and Mattaj,I.W.
  TITLE     PHAX, a mediator of U snRNA nuclear export whose activity is
            regulated by phosphorylation
  JOURNAL   Cell 101 (2), 187-198 (2000)
   PUBMED   10786834
REFERENCE   10 (bases 1 to 2054)
  AUTHORS   Flaherty,S.M., Fortes,P., Izaurralde,E., Mattaj,I.W. and
            Gilmartin,G.M.
  TITLE     Participation of the nuclear cap binding complex in pre-mRNA 3'
            processing
  JOURNAL   Proc Natl Acad Sci U S A 94 (22), 11893-11898 (1997)
   PUBMED   9342333
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY035864.1, AK077841.1, AK147027.1 and AI843301.1.
            
            On Apr 15, 2009 this sequence version replaced NM_026554.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147027.1, SRR1660811.120494.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                BY035864.1         35-127
            94-1666             AK077841.1         2-1574
            1667-2035           AK147027.1         1599-1967
            2036-2054           AI843301.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 22.4 cM"
     gene            1..2054
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="nuclear cap binding protein subunit 2"
                     /db_xref="GeneID:68092"
                     /db_xref="MGI:MGI:1915342"
     exon            1..182
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..575
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="nuclear cap binding protein subunit 2, 20kDa;
                     CBP20; NCBP 20 kDa subunit; 20 kDa nuclear cap-binding
                     protein"
                     /codon_start=1
                     /product="nuclear cap-binding protein subunit 2"
                     /protein_id="NP_080830.1"
                     /db_xref="CCDS:CCDS28110.1"
                     /db_xref="GeneID:68092"
                     /db_xref="MGI:MGI:1915342"
                     /translation="MSGGLLKALRSDSYVELSEYRDQHFRGDNEEQEKLLKKSCTLYV
                     GNLSFYTTEEQIYELFSKSGDIKKIIMGLDKMKKTACGFCFVEYYSRADAENAMRYIN
                     GTRLDDRIIRTDWDAGFKEGRQYGRGRSGGQVRDEYREDYDAGRGGYGKLAQKQ"
     exon            183..364
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            365..503
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            504..2037
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1724..1729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1750
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="major polyA site"
     regulatory      1894..1899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1914
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
     regulatory      2018..2023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2037
                     /gene="Ncbp2"
                     /gene_synonym="20kDa; 5930413E18Rik"
ORIGIN      
        1 ggctcgcccc gtggttctgg agcacgccgc ccgcatttcc gtttccgccg cccgccgctt
       61 ggttctcccg gccgtggccg tgtcgggttt ggtgtttctg catcatgtcg ggtggcttac
      121 tgaaggcgct gcgcagcgac tcctacgtgg aactcagcga gtaccgggac cagcactttc
      181 ggggcgacaa tgaagaacaa gaaaagttac tgaagaaaag ctgtacgttg tatgttggaa
      241 atctttcctt ttatacaact gaagaacaga tttatgaact cttcagcaaa agtggtgata
      301 taaagaagat catcatgggt ctggataaaa tgaagaaaac agcatgtggg ttctgttttg
      361 tggaatacta ttcaagagca gatgcggaga acgcaatgcg gtacataaac ggaactcgtc
      421 tggatgaccg gatcatacgg acagactggg atgcaggctt taaggagggc aggcagtatg
      481 gacgtggacg gtctgggggt caggttcggg acgagtatcg ggaggactac gatgctggaa
      541 gaggcggcta tgggaaactg gcacaaaaac agtgagtggg gtgagcccca agtacacagg
      601 tcacggtaca tcaacacagt ggtgatgctg gagaacgtta cccaaggtct catcagtgtc
      661 tctgctgtac tggaagccac ctctggtttt cctctgaaat attaaaagac agaatccaaa
      721 gaacgccttt aactctagtc ttattaggat gattttctat tattaggtca aaaagctcta
      781 cccaggcaac agacacaatc tgtgctgatt aaactgtcag ctggatacca gacttttagg
      841 gagactggac aaacatcagg actggctttt ataagtaggc aggtaatctt catggggact
      901 gaggtgattt tcttgtacgt tttacgtttt cctatttacc agtggggttt tgctgtagcc
      961 tgtgtaactc tgctctgagg aacaggtgtt tcccaactgg attcgccttt tctagtccag
     1021 tagagttggc accaatccag ccttaaatgg tcctggggat gccacctgct tctatgcttt
     1081 atgcatcagg atactctcag gacgtgagtg ttcacaagac acgtttccca aaaaatgcaa
     1141 aggtttcact gaaaatttaa atcaaaacaa aacaaaaaca aagaccttgt tacacagaga
     1201 gcctccgata tcctaagtag ggaggacact gtcagtaggg aacggaatga aaactcaaat
     1261 gttgcctgca gttgagcaag gaagctgagg ttggaaccat taaactattt cttcccattt
     1321 tcaaaaggac cttagagtag agtgggcttt agaaaagggc ttcacccagc tgcttgggag
     1381 gctgaagcaa gatgatcaaa aggtaaaggc cagcccgggg ccttgtgaat ctgcctcaca
     1441 atcggaactt aaaggggcag gggaaggtgc caggcacagc agttaaggac ttattatgtg
     1501 agtgttttgc ctttaatcct tggggttcaa caataaaaga gtctgaaaat tgggagagaa
     1561 aacctactct aggagcctct ggttacacat attttaggct ttttttcccc ctgaaattgt
     1621 acttactgag gtccatgtat taataagagg tcagtctgta caaaaatcta aagatttttt
     1681 cactttcttt gtattattat atatgagtaa cttttgatac cagaataaat ctttcgtgtt
     1741 tgtcactaaa gcatcggagt ctcgctagtt tctcatggtt gggtcacagc atccttgtcg
     1801 ctgtattaag tgcagttttc ttggagccat aacaactgtt aatgcttagg aagcccattt
     1861 gcaaacactc agtgaagaga cacttaagtt taaaataaag ctttttatac tgtagcttga
     1921 actgtttacc atacattctt ttgtagtaaa atataagtca gatttcaatc atctatgatt
     1981 caagacagtg ggaattttat cttctgtaac aagtttcaat aaagttagac cagcttcaaa
     2041 aaaaaaaaaa aaaa
//
