LOCUS       NM_001271601            3270 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus non-SMC condensin II complex, subunit H2 (Ncaph2),
            transcript variant 3, mRNA.
ACCESSION   NM_001271601
VERSION     NM_001271601.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Macdonald,L., Taylor,G.C., Brisbane,J.M., Christodoulou,E.,
            Scott,L., von Kriegsheim,A., Rossant,J., Gu,B. and Wood,A.J.
  TITLE     Rapid and specific degradation of endogenous proteins in mouse
            models using auxin-inducible degrons
  JOURNAL   Elife 11, e77987 (2022)
   PUBMED   35736539
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3270)
  AUTHORS   Houlard,M., Cutts,E.E., Shamim,M.S., Godwin,J., Weisz,D., Presser
            Aiden,A., Lieberman Aiden,E., Schermelleh,L., Vannini,A. and
            Nasmyth,K.
  TITLE     MCPH1 inhibits Condensin II during interphase by regulating its
            SMC2-Kleisin interface
  JOURNAL   Elife 10, e73348 (2021)
   PUBMED   34850681
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3270)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3270)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3270)
  AUTHORS   Theodoratos,A., Wilson,L.O., Gosling,K.M. and Fahrer,A.M.
  TITLE     Splice variants of the condensin II gene Ncaph2 include alternative
            reading frame translations of exon 1
  JOURNAL   FEBS J 279 (8), 1422-1432 (2012)
   PUBMED   22333158
  REMARK    GeneRIF: We confirm that Ncaph2 can generate three different
            N-termini, all encoded by exon 1, one of which is translated from
            an alternative reading frame.
REFERENCE   7  (bases 1 to 3270)
  AUTHORS   Rawlings,J.S., Gatzka,M., Thomas,P.G. and Ihle,J.N.
  TITLE     Chromatin condensation via the condensin II complex is required for
            peripheral T-cell quiescence
  JOURNAL   EMBO J 30 (2), 263-276 (2011)
   PUBMED   21169989
REFERENCE   8  (bases 1 to 3270)
  AUTHORS   Gosling,K.M., Goodnow,C.C., Verma,N.K. and Fahrer,A.M.
  TITLE     Defective T-cell function leading to reduced antibody production in
            a kleisin-beta mutant mouse
  JOURNAL   Immunology 125 (2), 208-217 (2008)
   PUBMED   18397266
REFERENCE   9  (bases 1 to 3270)
  AUTHORS   Gosling,K.M., Makaroff,L.E., Theodoratos,A., Kim,Y.H., Whittle,B.,
            Rui,L., Wu,H., Hong,N.A., Kennedy,G.C., Fritz,J.A., Yates,A.L.,
            Goodnow,C.C. and Fahrer,A.M.
  TITLE     A mutation in a chromosome condensin II subunit, kleisin beta,
            specifically disrupts T cell development
  JOURNAL   Proc Natl Acad Sci U S A 104 (30), 12445-12450 (2007)
   PUBMED   17640884
  REMARK    GeneRIF: define a unique lineage-specific requirement for kleisin
            beta in mammalian T cell differentiation
REFERENCE   10 (bases 1 to 3270)
  AUTHORS   Onn,I., Aono,N., Hirano,M. and Hirano,T.
  TITLE     Reconstitution and subunit geometry of human condensin complexes
  JOURNAL   EMBO J 26 (4), 1024-1034 (2007)
   PUBMED   17268547
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC113976.13.
            
            On Sep 9, 2022 this sequence version replaced NM_001271601.1.
            
            Summary: This gene encodes a component of the condensin-2 complex.
            The encoded protein may regulate the structure of mitotic
            chromosomes. Loss of function of this gene disrupts T-cell
            development. There are two pseudogenes for this gene on chromosome
            17. Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Oct 2012].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site, compared to variant 1. The encoded isoform (c) is
            longer than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165250.1, SRR17253011.8457664.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               AC113976.13        133553-133727
            176-277             AC113976.13        138140-138241
            278-333             AC113976.13        141215-141270
            334-418             AC113976.13        141389-141473
            419-487             AC113976.13        141550-141618
            488-567             AC113976.13        141706-141785
            568-722             AC113976.13        141872-142026
            723-806             AC113976.13        142397-142480
            807-904             AC113976.13        143018-143115
            905-976             AC113976.13        147168-147239
            977-1043            AC113976.13        147400-147466
            1044-1151           AC113976.13        147592-147699
            1152-1205           AC113976.13        147809-147862
            1206-1276           AC113976.13        148022-148092
            1277-1370           AC113976.13        148179-148272
            1371-1445           AC113976.13        148385-148459
            1446-1501           AC113976.13        148537-148592
            1502-1603           AC113976.13        148676-148777
            1604-1753           AC113976.13        148878-149027
            1754-3270           AC113976.13        149095-150611
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 44.84 cM"
     gene            1..3270
                     /gene="Ncaph2"
                     /note="non-SMC condensin II complex, subunit H2"
                     /db_xref="GeneID:52683"
                     /db_xref="MGI:MGI:1289164"
     exon            1..175
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1894
                     /gene="Ncaph2"
                     /note="isoform c is encoded by transcript variant 3;
                     condensin-2 complex subunit H2; kleisin beta"
                     /codon_start=1
                     /product="condensin-2 complex subunit H2 isoform c"
                     /protein_id="NP_001258530.1"
                     /db_xref="CCDS:CCDS70661.1"
                     /db_xref="GeneID:52683"
                     /db_xref="MGI:MGI:1289164"
                     /translation="MEDVEVRFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISF
                     DEGKTTMNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSLVQEDGS
                     KKTVNSETPCETENEFLSLDDFPDSRANVDLKNDQASSELLIIPLLPMALVAPDEVEK
                     NSSPLYSCQGDILASRKDFRMNTCMPNPRGCFMLDPVGMCPVEPVVPVEPYPMSRSQK
                     DPEDAEEQPMEVSRNGSPVPVPDISQEPDGPALSGGEEDAEDGAEPLEVALEPAEPRT
                     SQQSAILPRRYMLRERQGAPEPASRLQETPDPWQSLDPFDSLESKVFQKGKPYSVPPG
                     VEEAPGQKRKRKGATKLQDFHKWYLDAYAEHPDGRRARRKGPTFADMEVLYWKHVKEQ
                     LETLQKLRRRKINERWLPGAKQDLWPTEEDRLEESLEDLGVAADDFLEPEEYVEEPAG
                     VMPEEAADLDAEAMPESLRYEELVRRNVELFIATSQKFIQETELSQRIRDWEDTIQPL
                     LQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFSELVAGQPAFEVCRSMLASLQLANDY
                     TVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP"
     exon            176..277
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            278..333
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            334..418
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            419..487
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..567
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            568..722
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..806
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            807..904
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            905..976
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1043
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1151
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1205
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1276
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1370
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1445
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1501
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1603
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1753
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..3270
                     /gene="Ncaph2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2004..2009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncaph2"
                     /note="hexamer: AATAAA"
     polyA_site      2023
                     /gene="Ncaph2"
                     /note="major polyA site"
     regulatory      3249..3254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ncaph2"
                     /note="hexamer: AATAAA"
     polyA_site      3270
                     /gene="Ncaph2"
ORIGIN      
        1 accaaaatgg cgccagaacc agtagcgggt tgagccatgg ttccgggctt ctagtcccgt
       61 tctagacatg gaggatgtgg aggtgcgctt tgctcacctc ttgcagccca tccgggatct
      121 cactaagaac tgggaggtgg acgtggcggc acagctgggc gagtatctgg aggagctgga
      181 ccagatctgc atttcttttg atgaaggcaa aaccacaatg aacttcattg aggcagcact
      241 gttgatccag ggctcagcct gtgtctacag taagaaggtg gagtacctct actcgctggt
      301 ctaccaggct ctcgatttta tttctggcaa gaggcgggcc aaacagctct ccttagttca
      361 ggaagatggg agcaagaaga ctgtcaactc agagactccc tgtgaaacag agaatgagtt
      421 cctgtcactg gacgacttcc ctgactcccg ggctaatgtg gatctgaaaa atgatcaggc
      481 atccagtgag ctgcttatca tacccctact gcccatggcc ctggtggccc ctgatgaagt
      541 ggaaaagaac agcagcccct tgtatagctg tcagggtgac atcttggcca gccggaagga
      601 tttcaggatg aacacgtgta tgcctaaccc cagaggctgc tttatgttag atccagtggg
      661 aatgtgtcct gtggagcctg tggtgcccgt ggagccatac cccatgtcaa ggagccagaa
      721 agatcctgag gacgctgagg agcagcccat ggaagtgtct aggaacggga gtcctgttcc
      781 tgtacccgac atctcccaag agccagatgg tccagcgctc agcggtggag aggaggatgc
      841 agaggatgga gcagagcccc tggaggttgc tctagagcct gcagagccaa ggacctcaca
      901 gcagagtgcc atcttgccaa ggagatacat gctgcgggaa cgacaagggg caccggagcc
      961 tgcctcccgg ctacaggaga ccccagaccc ctggcagagc ctggaccctt ttgactcctt
     1021 ggaatctaag gtcttccaga aagggaaacc ctattctgtg ccacccggtg tggaggaggc
     1081 tccaggacag aagcgcaaga ggaagggtgc caccaagttg caggacttcc acaagtggta
     1141 cctggatgcc tatgctgaac accctgacgg caggagggct cggcggaagg gcccaacctt
     1201 tgcagacatg gaagtcctgt actggaaaca tgtgaaagaa cagctcgaga cccttcagaa
     1261 gctgcggaga cgtaagatca acgagagatg gctacctggg gccaagcagg atctgtggcc
     1321 tacagaggag gatcgcttgg aagagtccct cgaggacctc ggggtagcag cagatgactt
     1381 tctagagccc gaggagtacg tggaggagcc tgcgggggtg atgcccgagg aagctgctga
     1441 cctcgatgca gaggccatgc cagagtccct gagatacgag gagctggtcc ggagaaatgt
     1501 ggaactcttc attgccacct cccagaagtt tatccaggag acagagctga gccaacgcat
     1561 cagggactgg gaagatacca tccagcccct gctccaggag caggagcagc atgtgccctt
     1621 tgatatccat atctacgggg accagttggc ttcacggttc ccccagctca atgaatggtg
     1681 tcccttttca gagcttgtag cagggcagcc tgcttttgag gtgtgccgct ccatgctggc
     1741 ctccttgcaa ctggctaatg actacacagt ggagatcact cagcagccag gactggaggc
     1801 agctgtggac acaatgtctc tgagactgct cacacaccag cgagcccaca cccgcttcca
     1861 gacctatgct gcaccatcca tggcccagcc ttgagtggac agcactgagg caggggtgga
     1921 aagtagtata tacctggagg tctttgcccc taatgtgcta tggggccatt cactccagtg
     1981 ctgcctcctg gctggcctag cctaataaag tgttgctacc ccacctgttc accggacaga
     2041 ctatttaaat gagctgctgg tacagagcac atgcacagat taagtacatc catttaatga
     2101 cagggcctag gcaataggta tagttcaggg caagacactg tggaaggcag ctatgtgccg
     2161 acgtatactc tctacgatct gctctgctga cctgctgcga ccatagtaat cggtgaaaag
     2221 accatcaggg ttgagcagat agatggcaat ggagtggtcc acaatgtagt cctggtcctc
     2281 atccttaggc ccagcgctgt agtatacgcg gtagttgcga ctagcatggg ccacctgttc
     2341 tgtagaaccg gtcagaccta acagtcttgg gtggaattct tgcacatagc gggccatggc
     2401 tgccacgtca tctcgttctg ggtccacagt gatgaagaca ggctgcacca ggggcaggtc
     2461 aggctccgcc tctagcttcc gcaccacctg cactagcttt tccagctcat cggggcaaat
     2521 atcagggcag tgagtaaaac cgaagtacat cagcacccac tggcctcgga agtcggcttt
     2581 gcatcgaggc tggcctttgt ggtctagtag gctgaagtca ccctggccca cagcagcctg
     2641 gcgcagggcc tctgtacgct gctgttggcg ccactgttcc ttctcagccc tcgcggccag
     2701 ccaggcccag cctagcccag ccccaaacag ggctgtgatc agcagtctag ttcttagccc
     2761 agggccttgg cgctggccct gccctgccca gtgtcgggaa tttctatgca gagtttcacc
     2821 tcctgagatc ctgaggaggg gtttgaactg agagagcttg ggccaagctt tgggccctag
     2881 agccagtagc atcgtggacc tgaagctcct ggaaaagaac aaaggtggca aaagtcagaa
     2941 aagaatggac aagatgtcta ggcctccgct gctggacaag catctgcccc acattcaagc
     3001 aggttatcta gctgcctgca ccggctcgtc tcaactcaga actgctctgc cgtaagctgc
     3061 atgcagtcca ggcaacacca aaagtccata gctggtttgc tctaacctac gtctttaacc
     3121 agggagtctc agcctcatga ccccaacctc tggagaaccc acgccacaac tgccaagcct
     3181 ccctgtggat tcttggaagc accgcgaata tgctttgcct gggcactggg atgcatgtgt
     3241 ctatttacaa taaattgttc tttataggga
//
