LOCUS       NM_001001985            6529 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus N-acetyltransferase 8-like (Nat8l), mRNA.
ACCESSION   NM_001001985 XM_194205
VERSION     NM_001001985.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Li,Y., Huang,M., Wang,M., Wang,Y., Deng,P., Li,C., Huang,J.,
            Chen,H., Wei,Z., Ouyang,Q., Zhao,J., Lu,Y. and Su,S.
  TITLE     Tumor cells impair immunological synapse formation via central
            nervous system-enriched metabolite
  JOURNAL   Cancer Cell 42 (6), 985-1002 (2024)
   PUBMED   38821061
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   De Falco,P., Lazzarino,G., Felice,F., Desideri,E., Castelli,S.,
            Salvatori,I., Ciccarone,F. and Ciriolo,M.R.
  TITLE     Hindering NAT8L expression in hepatocellular carcinoma increases
            cytosolic aspartate delivery that fosters pentose phosphate pathway
            and purine biosynthesis promoting cell proliferation
  JOURNAL   Redox Biol 59, 102585 (2023)
   PUBMED   36580805
  REMARK    GeneRIF: Hindering NAT8L expression in hepatocellular carcinoma
            increases cytosolic aspartate delivery that fosters pentose
            phosphate pathway and purine biosynthesis promoting cell
            proliferation.
REFERENCE   3  (bases 1 to 6529)
  AUTHORS   Miyanishi,H., Kitazawa,A., Izuo,N., Muramatsu,S.I. and Nitta,A.
  TITLE     N-Acetyl Transferase, Shati/Nat8l, in the Dorsal Hippocampus
            Suppresses Aging-induced Impairment of Cognitive Function in Mice
  JOURNAL   Neurochem Res 47 (9), 2703-2714 (2022)
   PUBMED   35428956
  REMARK    GeneRIF: N-Acetyl Transferase, Shati/Nat8l, in the Dorsal
            Hippocampus Suppresses Aging-induced Impairment of Cognitive
            Function in Mice.
REFERENCE   4  (bases 1 to 6529)
  AUTHORS   Qi,L., Martin-Sandoval,M.S., Merchant,S., Gu,W., Eckhardt,M.,
            Mathews,T.P., Zhao,Z., Agathocleous,M. and Morrison,S.J.
  TITLE     Aspartate availability limits hematopoietic stem cell function
            during hematopoietic regeneration
  JOURNAL   Cell Stem Cell 28 (11), 1982-1999 (2021)
   PUBMED   34450065
REFERENCE   5  (bases 1 to 6529)
  AUTHORS   Becker,I., Wang-Eckhardt,L., Lodder-Gadaczek,J., Wang,Y.,
            Grunewald,A. and Eckhardt,M.
  TITLE     Mice deficient in the NAAG synthetase II gene Rimkla are impaired
            in a novel object recognition task
  JOURNAL   J Neurochem 157 (6), 2008-2023 (2021)
   PUBMED   33638175
REFERENCE   6  (bases 1 to 6529)
  AUTHORS   Ariyannur,P.S., Moffett,J.R., Manickam,P., Pattabiraman,N.,
            Arun,P., Nitta,A., Nabeshima,T., Madhavarao,C.N. and
            Namboodiri,A.M.
  TITLE     Methamphetamine-induced neuronal protein NAT8L is the NAA
            biosynthetic enzyme: implications for specialized acetyl coenzyme A
            metabolism in the CNS
  JOURNAL   Brain Res 1335, 1-13 (2010)
   PUBMED   20385109
  REMARK    GeneRIF: Asp-NAT enzyme activity in Nat8l-transfected cells is more
            than 300 times that of the mock-transfected controls and shows an
            extraordinarily high enzymatic specificity for aspartate, with less
            than 1% of enzymatic activity using glutamate as substrate.
            GeneRIF: Nat8l is highly specific to aspartate. It is localized in
            mitochondria and microsomes, with a pattern comparable to the
            localization of NAA in brain. It is similar to NAG synthetase in
            catalytic mechanism without the involvement of cysteine.
REFERENCE   7  (bases 1 to 6529)
  AUTHORS   Wiame,E., Tyteca,D., Pierrot,N., Collard,F., Amyere,M., Noel,G.,
            Desmedt,J., Nassogne,M.C., Vikkula,M., Octave,J.N., Vincent,M.F.,
            Courtoy,P.J., Boltshauser,E. and van Schaftingen,E.
  TITLE     Molecular identification of aspartate N-acetyltransferase and its
            mutation in hypoacetylaspartia
  JOURNAL   Biochem J 425 (1), 127-136 (2009)
   PUBMED   19807691
  REMARK    GeneRIF: Results indicated that NAT8L, but not NAT14, catalysed the
            synthesis of NAA from L-aspartate and acetyl-CoA.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6529)
  AUTHORS   Niwa,M., Nitta,A., Cen,X., Kitaichi,K., Ozaki,N., Yamada,K. and
            Nabeshima,T.
  TITLE     A novel molecule 'shati' increases dopamine uptake via the
            induction of tumor necrosis factor-alpha in pheochromocytoma-12
            cells
  JOURNAL   J Neurochem 107 (6), 1697-1708 (2008)
   PUBMED   19014384
REFERENCE   9  (bases 1 to 6529)
  AUTHORS   Niwa,M., Nitta,A., Mizoguchi,H., Ito,Y., Noda,Y., Nagai,T. and
            Nabeshima,T.
  TITLE     A novel molecule 'shati' is involved in methamphetamine-induced
            hyperlocomotion, sensitization, and conditioned place preference
  JOURNAL   J Neurosci 27 (28), 7604-7615 (2007)
   PUBMED   17626222
  REMARK    GeneRIF: Roles of shati in methamphetamine (METH)-regulated
            behavioral alternations are likely to be mediated by its inhibitory
            effects on METH-induced increase of dopamine(DA) overflow in
            nucleus accumbens and METH-induced decrease in DA uptake in
            midbrain.
REFERENCE   10 (bases 1 to 6529)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163329.6 and AK147594.1.
            
            On Mar 30, 2007 this sequence version replaced NM_001001985.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147307.1, AK147703.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-722               AC163329.6         10174-10895         c
            723-2894            AK147594.1         2-2173
            2895-6529           AC163329.6         963-4597            c
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.83 cM"
     gene            1..6529
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /note="N-acetyltransferase 8-like"
                     /db_xref="GeneID:269642"
                     /db_xref="MGI:MGI:2447776"
     exon            1..1227
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /inference="alignment:Splign:2.1.0"
     CDS             861..1760
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /EC_number="2.3.1.17"
                     /note="N-acetyltransferase 8-like protein; protein Shati;
                     NAA synthetase; aspartate N-acetyltransferase; NAA
                     synthase"
                     /codon_start=1
                     /product="N-acetylaspartate synthetase"
                     /protein_id="NP_001001985.3"
                     /db_xref="CCDS:CCDS19210.1"
                     /db_xref="GeneID:269642"
                     /db_xref="MGI:MGI:2447776"
                     /translation="MHCGPPDMVCETKIVATEDHEALPGAKKDALLVAAGAMWPPLPA
                     APGPAAAPPPAAGPQPHGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPN
                     TAFRGLRQHPRTQLLYALLAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLEC
                     ALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRG
                     KGIAKALGRRVLEFAMLHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMT
                     LSLAERLFFQVRYHRYRLQLREE"
     exon            1228..1392
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..6529
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3632..3637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /note="hexamer: ATTATA"
     polyA_site      3672
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
     regulatory      6508..6513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /note="hexamer: AATAAA"
     polyA_site      6529
                     /gene="Nat8l"
                     /gene_synonym="1110038O08Rik; Shati"
                     /note="major polyA site"
ORIGIN      
        1 gcggaaggcg ggggccgagc cgggaggggt cctgcaattc cgcaggtccg ctgccgggaa
       61 cggtcgggtg tcagattgga gtggggtggg gttcggctgt caggcagcgc gtgcatcaaa
      121 ccacatttca gccgcgtcct cactctctca gtccgttcgg ccccggccgc gggctcggag
      181 ggcgcctgcc aaaggaacac ggggcccctc acgaggactt cggccgggcg ccgcgtacag
      241 acaaaggggt gggagatgga gacacttccg tgattactgc cagcggccgc ggaagaagag
      301 gacgctttgc tgcaggaacg caggtccgag cgcagcggca gctttgcggg agctcagggg
      361 cggggtagga ggcgtccatc actctccgac ctcctggcga gcctcaagcc ccttacgcag
      421 cgcctctctg agcaccctgt ccctgctgtc gttcactgag gaccacttcc tcgcacgctc
      481 tccgttcggc ccctccgtgc tcccgcggtc cgccagccac gcagcccaag tgtgccatcg
      541 ggcgggcgca gaggcggcgt ctgctcccac ccccgagatc acatggtgac cctcggcagc
      601 caatgcagtg gacgctagga ccctgcggga ccccgggcgc cgcgactgca ccggcgccgc
      661 tgcattataa atacttctcc aaaatgcggc gcagctccct gcgcgcgccc cggccgcccg
      721 ccgcctccgc cctgcgcccc tgagccgctg ccgccgccgc tgccgccgcg ggtccgaggg
      781 cgccgcgccc ttgccctggg ctcgggctct gcccgctgcc gccggctcgc gtcctcgcgc
      841 tgcgccgccc ggccgggtgc atgcattgtg ggcctcccga catggtctgc gagacgaaga
      901 tcgtggctac ggaggaccat gaggcactgc cgggggccaa gaaggacgcg ctgctcgtcg
      961 ccgccggagc catgtggccc ccgctgcccg ccgcgcccgg gccggccgcc gcacccccac
     1021 ccgcggcagg tccccagccc cacggaggca ccgggggcgc ggggccgccg gaggggcgcg
     1081 gcgtgtgcat ccgcgagttc cgcgcagccg agcaggaggc ggcgcgccgc atcttctacg
     1141 acggcatctt ggagcgcatc cccaacacgg ctttccgcgg cctgcggcag cacccgcgca
     1201 cccaactgct ctacgccctg ctggccgccc tctgttttgc tgtgacccgc tcactgctgc
     1261 tgacatgcct ggtgccggcc gggctcctgg ccctgcgcta ctactacagc cgcaaggtga
     1321 ttctggccta cctggagtgt gcgctgcaca cggacatggc tgacattgag cagtactaca
     1381 tgaagccacc tggttcctgt ttctgggtgg ctgtgctgga cggcaacgtt gtgggcatcg
     1441 tggccgcacg ggcccatgag gaggacaaca cagtggagct gctccgcatg tccgtggact
     1501 cgcgtttccg cggaaaaggc atcgccaagg ctctgggcag gagggtgttg gagtttgcca
     1561 tgctgcacaa ctactctgca gtggtactgg gcaccacagc cgttaaggtg gccgcccaca
     1621 agctctatga gtcactgggc ttcagacaca tgggcgcgag tgatcactac gtgctgcctg
     1681 gcatgaccct ctcgctggcc gagcgcctct tcttccaggt ccgctaccac cgctaccgcc
     1741 tgcagctgcg cgaggagtga ccaccaccac ctgcccacca ccctgtctgc ccccgtccac
     1801 ctctgccctc ttgctcactg cctagtgcgg ccctgctttc caatgctcac cttggcattt
     1861 gtttgggttt tcctttttca gcatcatgct ggttcatcac ctggctggtt ctggggccac
     1921 agggtcccag tccagagtgc tcatctgcaa cactctggtg gggtggattg tctgcagcca
     1981 tggcccttgc ctccccagcc catcagagtg gctcatcatg ccttgaagag cagcattgtg
     2041 gaccatctga actatccaca aagctgccca gcctgcctcc atcagggaag gactagcctt
     2101 gcccatcaag cacagaacct ctacaacaac cccccccccc ccaaggagca gaaccctggc
     2161 ccccagccag gctaaggctc aactattggc ctctggaaga gtggtctgct gggctggcca
     2221 gccactgccc catgggaggc tgagctggtc ccaggccctg ctttgccctg tgcatgctaa
     2281 ggacatggcc tggctgcagc atgccgcatg ccacagcccc ctgccccact atcctgccca
     2341 gcctggacct caggcctgag ctggtgagct cccttgtcct gtcctgagct ggcctctgga
     2401 gggcctacct gcctgaccct gtctactctc tggtgaggcc tggcctggcc taacctggct
     2461 ctatccactc ctgctttgct tcgaaatcat cttacctctt tccctttttg gtccccgtgc
     2521 ctccttgctc tggctgactt ggctggtcac ccgggtttgg ttcctgtagc aatccactgc
     2581 acagcatggt ggggatttca tgggggtggg ggggacacct cccccaacac ctgttagacc
     2641 aggtggccac ctcccagctg cctgaggtcc tgtggcctca tgctgtttgt ggggtctgcc
     2701 ctgtgaatgt aactagccca ggctgttgtt ggcaatgcct gccacagctt ctcccagcct
     2761 gaaacaaccc attctctaat aagttacgta gacagaatag cactctgcat gactttaatt
     2821 actgggacaa aatggtagtt tgagcctcca gacacacatc tgtgtggtgc tagcatacgc
     2881 atggtctctg gccccatttg atgggggtgg gtggccgggt aggtggaaga tttcagtttg
     2941 aagtatggaa ggagactcta atttagtgta aacaccccta aagtgccctc acaacacagt
     3001 ctgtgaggct caggttgccc cttgattcct tgtatgcagg attgagggga ctggagggtc
     3061 ctaaagcctc ataggaaggg ctgggcactg cctcggggga agaagctggt ggttggggtg
     3121 ggtccaggta tcacccacta cagactactc tgctaggtca actgttcctg gcctctcctt
     3181 tttgaggttc tacttcagat ccagcctaga tgggggtggg tatggcctag gacagggaaa
     3241 ggcagtagct ggcagtggtc atgaggccag ctgaaacttg gacttagctt tagtgcaggg
     3301 ggtgcataaa ggcccgtcca cattcctctg tgtggggctt ggggtgagga gtggcaggct
     3361 aggtagacca tagagctggg cccctggcac caaggctaca ttattagaaa ggctctttag
     3421 agttaatgag ttggtggaac aagcccagct tcctgagggg ccttttgtcc tgttagcaat
     3481 tgaggcattt gcagaacact gtacagaccc cgctctcccc tgtacattcc tccctggtgg
     3541 tgcccggtcc ccgcttgggg atgggagttt tgtagactgt acagaaattg gcaccctatt
     3601 ttcttgcagc tctcagattt tgttaatctg gattatacag acagatgtaa agtgttttag
     3661 caaaatggaa aacaaacagt tgtgcctttt ttctcttttc tgtttggatt ggtctcggct
     3721 cggggctagt cttagttgtt gtgggggtat agggactgct ggtgtctgag gcctgtgggg
     3781 tcagagactg ctggtgggtc tgaggcctgg gtaggcctaa gtgggagcca ggtctactgg
     3841 acaccaccag ctgccaagac ttctctgatc ctgtgccttt ggggcctgca gtctggggtt
     3901 ggaaggtgtg gcagggcact gggatcactt gggccagggc tgagcttggg gatcctgtag
     3961 ccacccatgt ctccttgata ctgacctggg atgagctcat agtagattcc tccagcctag
     4021 gagcctggga gaagccgtgg cccttccagc ctcttggcac ctttccaagg ggatctctcc
     4081 ctgctgaaca acccatggat aaagggaagt ttgctgggct ctcaagtatt cgttgccttc
     4141 tgttgctgcc aacacttggt agagattcca ggaatggatg gtggggtagg ctccagctgc
     4201 atcaggatgg agcctcacca ggcagtatgt agcttggcag ggcctgaggc cctggagaca
     4261 tacctgtgtc cctgggctgg cggaagctga gtgtgctgat ggctgcgcct gactgtgtcc
     4321 actccatcat cccagccaga gctctgagtg ctctgcacat gtgcactgat gtgaattttc
     4381 tgtttgttga gattctgatg tttcttctac ctctgtggag gccctcctct ctgctccact
     4441 tggtggccat gtgcttggtg cccctcctct gagttggctg gccatctccc cctcccttga
     4501 gcctccaagc acctgaggtg gccagcagct gtactctggg cctgggaagg cccagggaag
     4561 gccagcctgg ggtctgactc tctgctcagg atctgtggct tccaggagtc ctattgtttg
     4621 cttggaaact ttagagtagg gagggaaaaa gagatgatgg aatgtcccag aatgtcagtg
     4681 ttggatgacc ctacagctca gatgtggtat accattgctt gactgttccc aggcagctcc
     4741 cactgcccag gcatgtgtct ctgcagatgc ctcgtctctg ctgtaggcca agaggctggc
     4801 ctgcatgttc taagatctgg cccctgagtg tagcgaggcc atgccctggt ggccatgaca
     4861 gcctcttcca tgtcctggtc tcacccctat gcttatcctt ccccctagca ctgccctggg
     4921 gctgggcagc agctaaaggc atgtgctaac ctggctgagg tggtagatgg ctggctttgg
     4981 ctgggaggcc ctgtggttaa caacttagtt tcagggtctt ggacagttcc catcttgcac
     5041 aggtaccctt cctgagataa gggatagggc agaggacaga gtcagggaca cacctaaagt
     5101 gagcttgggc tttgacctga tgcctgggac tcgctagacc ataggatcat cgcttgttgc
     5161 tggaaaacat gctgcccatt cctacttgcc tttgccaacc ccaggtagcc ccatggtcac
     5221 agcttcctgg agctggcgtc cagggctgtg cctgtggcta aaggcctggg gacaaaactt
     5281 tttgggtctt gctggtgtcc caggtttctt ggggactgga tgtgacctcc tagctgcgta
     5341 ggaactgtgc agcacccatg aggtcatctt cttgtcaggc ccaaggggca gcctgagaga
     5401 gctgacctga ggtggctgtg tccctggcca tggtagcagt gctgctggcg cccgttgtcc
     5461 ttggcttttg gccaccctct gctgggtgtg aggcagaccc tgggctgggc tgggctgctc
     5521 tttggtgcta ctgtcaccgt gcttccagca agggaggggt ccatgatggt gggtgggact
     5581 tgcctggagc tgggtggaag atgagccttg acttaccact gttggatgta ttttttttta
     5641 aaacagcaat aattagccat tttaaagaag gggtttacct acataaatgt gcatgcccat
     5701 cggtgtatgt gcatttgtgc acatgtatgt gcatctgaga gtatgtgttt gcatgtgaga
     5761 gcatctgtgt gtgtgtagga actttgcagc acccacaggg ccatcttctt gtcaggccca
     5821 aggggcagcc tgagagagtt gaccgcgaca cacacacaca cacacacaca cacacacaca
     5881 cacacgccat ttgtgatcat gtgctcatat gactatatgt gcccaagaaa tcacattcat
     5941 gtgttgcatg tgtgtgagca cgtttgagta tgtgtgggca tctccatatg tgtatgaatg
     6001 tgtgcatgtg tatgcttgta cgtgcacatg tatagcagtg ggagggagtc tgtggggaca
     6061 gtctctgatc tgggaggcac tttgtggtaa gaagggtctt gtggttgtct ttcactatgt
     6121 ccattccctg ccctgccctg ccctgcccag ctccacgtga atgtagcaca accccaccag
     6181 gccagctgag cctctgctgg ccctcacctc tgtccatgtc cccctcagct gagggtaggg
     6241 tagaggagct ggagttccct tggataggac actgactggg tttggccagg gtgcagaggg
     6301 tatgacacag tggtaggtct cactgttgtg gtttgtttct gaatttgtac tgtgagccca
     6361 tgatgggggt gggggtgtcc tgagacccca ggatgtgcca aaatagcccc tagggccagg
     6421 tgacgagccg agttcccaag aagacaggct gggggcccaa gtgggtgtgt acccctgcct
     6481 gcatggcccc caatgctgct gtttttcaat aaaaccagaa ttgaaggaa
//
