LOCUS       NM_001168577            1375 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase)
            (Nat2), transcript variant 2, mRNA.
ACCESSION   NM_001168577
VERSION     NM_001168577.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Denis,R.G.P., Busi,F., Castel,J., Morel,C., Zhang,W., Bui,L.C.,
            Sugamori,K.S., Prokopec,S.D., Boutros,P.C., Grant,D.M.,
            Rodrigues-Lima,F., Luquet,S. and Dupret,J.M.
  TITLE     A readout of metabolic efficiency in arylamine
            N-acetyltransferase-deficient mice reveals minor energy metabolism
            changes
  JOURNAL   FEBS Lett 593 (8), 831-841 (2019)
   PUBMED   30883722
  REMARK    GeneRIF: metabolic efficiency in arylamine
            N-acetyltransferase-deficient mice
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Yang,C.L., Zheng,X.L., Ye,K., Sun,Y.N., Lu,Y.F., Ge,H. and Liu,H.
  TITLE     Effects of microRNA-217 on proliferation, apoptosis, and autophagy
            of hepatocytes in rat models of CCL4-induced liver injury by
            targeting NAT2
  JOURNAL   J Cell Physiol 234 (4), 3410-3424 (2019)
   PUBMED   30417525
  REMARK    GeneRIF: miR-217 inhibits proliferation adn promotes apoptosis and
            autophagy in hepatocytes in CCL4-induced liver injury through
            targeting NAT2 expression.
REFERENCE   3  (bases 1 to 1375)
  AUTHORS   Wang,P., Shehu,A.I., Lu,J., Joshi,R.H., Venkataramanan,R.,
            Sugamori,K.S., Grant,D.M., Zhong,X.B. and Ma,X.
  TITLE     Deficiency of N-acetyltransferase increases the interactions of
            isoniazid with endobiotics in mouse liver
  JOURNAL   Biochem Pharmacol 145, 218-225 (2017)
   PUBMED   28888949
  REMARK    GeneRIF: This study illustrated that deficiency of NAT1/2 decreases
            isoniazid (INH) acetylation, but increases the interactions of INH
            with endobiotics in the liver.
REFERENCE   4  (bases 1 to 1375)
  AUTHORS   Ramirez-Alcantara,V. and Montrose,M.H.
  TITLE     Acute murine colitis reduces colonic 5-aminosalicylic acid
            metabolism by regulation of N-acetyltransferase-2
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 306 (11), G1002-G1010
            (2014)
   PUBMED   24742986
  REMARK    GeneRIF: It was concluded that an acute colonic inflammation
            impairs the expression and function of NAT2 enzyme, thereby
            diminishing the capacity for 5-aminosalisylic acid metabolism by
            colonic mucosa.
REFERENCE   5  (bases 1 to 1375)
  AUTHORS   Laurieri,N., Dairou,J., Egleton,J.E., Stanley,L.A., Russell,A.J.,
            Dupret,J.M., Sim,E. and Rodrigues-Lima,F.
  TITLE     From arylamine N-acetyltransferase to folate-dependent acetyl CoA
            hydrolase: impact of folic acid on the activity of (HUMAN)NAT1 and
            its homologue (MOUSE)NAT2
  JOURNAL   PLoS One 9 (5), e96370 (2014)
   PUBMED   24823794
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1375)
  AUTHORS   Vatsis,K.P., Weber,W.W., Bell,D.A., Dupret,J.M., Evans,D.A.,
            Grant,D.M., Hein,D.W., Lin,H.J., Meyer,U.A., Relling,M.V. et al.
  TITLE     Nomenclature for N-acetyltransferases
  JOURNAL   Pharmacogenetics 5 (1), 1-17 (1995)
   PUBMED   7773298
  REMARK    Review article
REFERENCE   7  (bases 1 to 1375)
  AUTHORS   Kelly,S.L. and Sim,E.
  TITLE     Arylamine N-acetyltransferase in Balb/c mice: identification of a
            novel mouse isoenzyme by cloning and expression in vitro
  JOURNAL   Biochem J 302 (Pt 2) (Pt 2), 347-353 (1994)
   PUBMED   7545952
REFERENCE   8  (bases 1 to 1375)
  AUTHORS   Martell,K.J., Levy,G.N. and Weber,W.W.
  TITLE     Cloned mouse N-acetyltransferases: enzymatic properties of
            expressed Nat-1 and Nat-2 gene products
  JOURNAL   Mol Pharmacol 42 (2), 265-272 (1992)
   PUBMED   1513324
REFERENCE   9  (bases 1 to 1375)
  AUTHORS   Martell,K.J., Vatsis,K.P. and Weber,W.W.
  TITLE     Molecular genetic basis of rapid and slow acetylation in mice
  JOURNAL   Mol Pharmacol 40 (2), 218-227 (1991)
   PUBMED   1875909
REFERENCE   10 (bases 1 to 1375)
  AUTHORS   Ebihara,S., Marks,T., Hudson,D.J. and Menaker,M.
  TITLE     Genetic control of melatonin synthesis in the pineal gland of the
            mouse
  JOURNAL   Science 231 (4737), 491-493 (1986)
   PUBMED   3941912
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK007583.1 and AC122867.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012972.2, AK007583.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-680               AK007583.1         10-689
            681-1375            AC122867.4         176608-177302
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 33.38 cM"
     gene            1..1375
                     /gene="Nat2"
                     /note="N-acetyltransferase 2 (arylamine
                     N-acetyltransferase)"
                     /db_xref="GeneID:17961"
                     /db_xref="MGI:MGI:109201"
     exon            1..28
                     /gene="Nat2"
                     /inference="alignment:Splign:2.1.0"
     exon            29..1375
                     /gene="Nat2"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..907
                     /gene="Nat2"
                     /EC_number="2.3.1.5"
                     /EC_number="2.3.1.118"
                     /note="N-acetyl transferase 2; NAT-2; arylamide acetylase
                     2; N-acetyltransferase type 2; N-hydroxyarylamine
                     O-acetyltransferase"
                     /codon_start=1
                     /product="arylamine N-acetyltransferase 2"
                     /protein_id="NP_001162049.1"
                     /db_xref="CCDS:CCDS22338.1"
                     /db_xref="GeneID:17961"
                     /db_xref="MGI:MGI:109201"
                     /translation="MDIEAYFERIGYQSTRSKLDLKTLTEILQHQIRAIPFENLNIHC
                     GESMELSLEAIFDQIVRKKRGGWCLQVNHLLYWALTKLGFETTMLGGYVFNTPANKYS
                     SGMIHLLVQVTISGKDYIVDAGFGRSYQMWEPLELTSGKDQPQVPAIFRLTEENGTWY
                     LDQIRREQYVPNQEFINSDLLEKNKYRKIYSFTLEPRTIEDFESMNTYLQTSPASVFT
                     SKSFCSLQTPEGVHCLVGSTLTYRRFSYKDNVDLVEFKSLTEEEIEDVLRTIFGVSLE
                     RKLVPKHGDRFFTI"
     regulatory      1361..1366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nat2"
                     /note="hexamer: AATATA"
     polyA_site      1375
                     /gene="Nat2"
                     /note="major polyA site"
ORIGIN      
        1 gtccgcccgg ttgcagtcct ggtagcggga aaccatggac atcgaagcgt actttgaaag
       61 aattggttat cagagcacca ggagcaaact ggacttgaaa acattaaccg aaatccttca
      121 gcaccagata cgggctattc cctttgagaa tttgaacatc cattgtgggg aatccatgga
      181 actgagttta gaagccatct ttgatcaaat tgtgaggaag aagcggggtg gatggtgtct
      241 ccaggttaat catctgctgt actgggctct gaccaaactg ggctttgaaa ccacaatgct
      301 gggaggatat gtctttaaca ctccagccaa taagtacagc agtggcatga ttcaccttct
      361 agttcaggtc accatcagtg gaaaggacta cattgttgat gctgggtttg gacgttccta
      421 ccagatgtgg gagcctctgg aattaacatc tggaaaggat cagcctcagg tgcctgccat
      481 cttccgtttg acagaggaga atggaacctg gtacttggac caaatcagaa gagagcagta
      541 tgttccaaac caagaattta ttaactcaga tctccttgaa aagaacaaat accgaaaaat
      601 ctattccttt actcttgagc cccgaactat tgaagatttt gagtccatga atacctacct
      661 tcagacatca ccagcgtctg tgtttactag caaatcattt tgttccctgc agaccccaga
      721 aggagttcat tgtttggttg gctccaccct cacttataga agatttagtt acaaggacaa
      781 cgtcgatctt gtagagttta agagtctgac tgaggaagaa atagaagatg tactgagaac
      841 tatatttggg gtttctttag agagaaaact tgtgcctaaa catggtgatc gattttttac
      901 catttagaat atgtaaagtt ttggtgtcct tccatgtact tggaattttt atgataagat
      961 attcaaactg gtgacatgat catactactg gtggtcatgt tgatgtgtgc taggaaatac
     1021 ccagcattgg cttacagcta aatttatata gaatttgtgc taattgttaa gtaaaaagaa
     1081 tagtgtgaaa agcattccca ttaaattcaa ccagcattat cccatatcaa atttttatga
     1141 cttattacct gtacaattta ggatttggat tcaacaaaga agaatgagaa gtgtatgtga
     1201 ttagggccca agaaaaccag tcacacagct aattatttct ttctgtgttg tttttatatc
     1261 tacttttttt atatactatt ttttcttact gtgggaacaa acaccaattg agtaagttga
     1321 gcttgatttt aaaaatgtaa gtctaaatta ataaaaatga aatatatatc taaaa
//
