LOCUS       NM_016777               3087 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus nuclear autoantigenic sperm protein (histone-binding)
            (Nasp), transcript variant 2, mRNA.
ACCESSION   NM_016777
VERSION     NM_016777.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Qiu,Y., Pei,D., Wang,M., Wang,Q., Duan,W., Wang,L., Liu,K., Guo,Y.,
            Luo,L., Guo,Z., Guan,F., Wang,Z., Xing,A., Liu,Z., Ma,Z., Jiang,G.,
            Yan,D., Liu,X., Zhang,Z. and Wang,W.
  TITLE     Nuclear autoantigenic sperm protein facilitates glioblastoma
            progression and radioresistance by regulating the ANXA2/STAT3 axis
  JOURNAL   CNS Neurosci Ther 30 (4), e14709 (2024)
   PUBMED   38605477
  REMARK    GeneRIF: Nuclear autoantigenic sperm protein facilitates
            glioblastoma progression and radioresistance by regulating the
            ANXA2/STAT3 axis.
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Bao,Y., Lian,M., Chen,Y., Gu,X., Cao,K., Du,X. and Ju,J.
  TITLE     sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE
            by TAK1 Pathway
  JOURNAL   J Immunol Res 2023, 4877700 (2023)
   PUBMED   37771504
  REMARK    GeneRIF: sNASP Mutation Aggravates to the TLR4-Mediated
            Inflammation in SLE by TAK1 Pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3087)
  AUTHORS   Li,J., Jiang,H., Mu,Y., Wei,Z., Ma,A., Sun,M., Zhao,J., Zhu,C. and
            Chen,X.
  TITLE     SRSF10 regulates proliferation of neural progenitor cells and
            affects neurogenesis in developing mouse neocortex
  JOURNAL   iScience 26 (7), 107042 (2023)
   PUBMED   37360696
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3087)
  AUTHORS   Zhang,Y., Yan,H., Zhu,J., Chen,L., Wang,H. and Ju,J.
  TITLE     [Hepatic fibrosis aggravation in nuclear autoantigenic sperm
            protein (NASP) mutant mice induced by concanavalin A]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 38 (7), 577-583 (2022)
   PUBMED   35786450
  REMARK    GeneRIF: [Hepatic fibrosis aggravation in nuclear autoantigenic
            sperm protein (NASP) mutant mice induced by concanavalin A].
REFERENCE   5  (bases 1 to 3087)
  AUTHORS   Zhang,J., Du,X., Wang,H., Bao,Y., Lian,M., Xu,Z. and Ju,J.
  TITLE     A Variant of sNASP Exacerbates Lymphocyte Subset Disorder and
            Nephritis in a Spontaneous Lupus Model Sle1.Yaa Mouse
  JOURNAL   Mediators Inflamm 2021, 8175863 (2021)
   PUBMED   34720750
  REMARK    GeneRIF: A Variant of sNASP Exacerbates Lymphocyte Subset Disorder
            and Nephritis in a Spontaneous Lupus Model Sle1.Yaa Mouse.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3087)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 3087)
  AUTHORS   Richardson,R.T., Bencic,D.C. and O'Rand,M.G.
  TITLE     Comparison of mouse and human NASP genes and expression in human
            transformed and tumor cell lines
  JOURNAL   Gene 274 (1-2), 67-75 (2001)
   PUBMED   11674998
REFERENCE   8  (bases 1 to 3087)
  AUTHORS   Richardson,R.T., Batova,I.N., Widgren,E.E., Zheng,L.X.,
            Whitfield,M., Marzluff,W.F. and O'Rand,M.G.
  TITLE     Characterization of the histone H1-binding protein, NASP, as a cell
            cycle-regulated somatic protein
  JOURNAL   J Biol Chem 275 (39), 30378-30386 (2000)
   PUBMED   10893414
REFERENCE   9  (bases 1 to 3087)
  AUTHORS   Hemberger,M., Himmelbauer,H., Ruschmann,J., Zeitz,C. and Fundele,R.
  TITLE     cDNA subtraction cloning reveals novel genes whose temporal and
            spatial expression indicates association with trophoblast invasion
  JOURNAL   Dev Biol 222 (1), 158-169 (2000)
   PUBMED   10885754
REFERENCE   10 (bases 1 to 3087)
  AUTHORS   Leimeister,C., Bach,A., Woolf,A.S. and Gessler,M.
  TITLE     Screen for genes regulated during early kidney morphogenesis
  JOURNAL   Dev Genet 24 (3-4), 273-283 (1999)
   PUBMED   10322635
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX004835.3 and AL669953.7.
            
            On Feb 9, 2007 this sequence version replaced NM_016777.2.
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF034610.2, SRR7345562.2481421.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                BX004835.3         3866-3927           c
            63-160              BX004835.3         3308-3405           c
            161-208             AL669953.7         176423-176470       c
            209-319             AL669953.7         172526-172636       c
            320-400             AL669953.7         167986-168066       c
            401-510             AL669953.7         165628-165737       c
            511-1485            AL669953.7         164059-165033       c
            1486-1565           AL669953.7         159340-159419       c
            1566-1651           AL669953.7         158565-158650       c
            1652-1725           AL669953.7         158397-158470       c
            1726-1914           AL669953.7         157819-158007       c
            1915-2081           AL669953.7         157551-157717       c
            2082-2138           AL669953.7         156544-156600       c
            2139-2216           AL669953.7         156382-156459       c
            2217-2345           AL669953.7         155748-155876       c
            2346-3087           AL669953.7         154708-155449       c
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 53.24 cM"
     gene            1..3087
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /note="nuclear autoantigenic sperm protein
                     (histone-binding)"
                     /db_xref="GeneID:50927"
                     /db_xref="MGI:MGI:1355328"
     exon            1..62
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            63..160
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..2423
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /note="isoform 2 is encoded by transcript variant 2;
                     somatic histone binding protein NASP"
                     /codon_start=1
                     /product="nuclear autoantigenic sperm protein isoform 2"
                     /protein_id="NP_058057.3"
                     /db_xref="CCDS:CCDS18513.1"
                     /db_xref="GeneID:50927"
                     /db_xref="MGI:MGI:1355328"
                     /translation="MATESTAAAAIAAELVSADKIEDAPAPSTSADKMESLDVDSEAK
                     KLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARME
                     NGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDEEAREELREQVYDAMGEKEAKKAE
                     GKSLTKPETDKEQESEVEKGGREDMDISEPEEKLQETVEPTSKQLTESSEEAKEAAIP
                     GLNEDEVASGKTEQESLCTEKGKSISGAYVQNKEFRETVEEGEEIISLEKKPKETSED
                     QPIRAAEKQGTLMKVVEIEAEIDPQVKSADVGGEEPKDQVATSESELGKAVLMELSGQ
                     DVEASPVVAAEAGAEVSEKPGQEITVIPNNGPVVGQSTVGDQTPSEPQTSAERLTETK
                     DGSSVEEVKAELVPEQEEAMLPVEESEAAGDGVETKVAQRATEKAPEDKFKIAANEET
                     QERDEQMKEGEETEGSEEEDRENDKAEETPNESVLEKKSLQENEEEEIGNLELAWDML
                     DLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQYLEAHD
                     RLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMAVLHEQMKEAEGSFTEYEKE
                     IEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGASVSMIAS
                     RKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVSSGKE
                     VSENMEAEAENQAESQTAEGTVESAATIKSTAC"
     exon            161..208
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            209..319
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            320..400
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            401..510
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            511..1485
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1565
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1651
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1725
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1914
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2081
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2138
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2216
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2345
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..3087
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2511..2516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /note="hexamer: AATAAA"
     polyA_site      2530
                     /gene="Nasp"
                     /gene_synonym="5033430J04Rik; D4Ertd767e; Epcs32; Nasp-T"
                     /note="major polyA site"
ORIGIN      
        1 agctcttgcg tttccatccg ttaagactga gaggacatag ggaatacctg tcaccttctc
       61 gggcagtgtt ctgcccactg gcccaccacc tcaggggaac gatggccaca gagtctacag
      121 ccgctgctgc catcgccgcg gagctggttt ccgctgacaa aattgaagat gctcctgctc
      181 cttctacctc ggcagataaa atggagagtc tggatgtgga tagtgaagct aagaaactac
      241 tgggattagg acagaaacat ctggtgatgg gtgacattcc agcagctgtt aatgcattcc
      301 aggaagcagc tagtctttta ggtaagaagt atggagaaac ggctaatgag tgtggagaag
      361 ccttcttttt ttatgggaag tcacttctgg agttagccag aatggagaat ggagtgttgg
      421 gaaatgcctt agaaggagtg catgtagaag aggaagaagg agaaaaaaca gaagatgaat
      481 ctttggtaga aaataatgat aatgtagatg aggaagcaag ggaagagttg agagaacagg
      541 tttatgacgc catgggagaa aaagaagcca aaaaagcaga aggcaagtct ctgacaaagc
      601 ctgaaactga taaagaacag gaaagtgaag tggagaaggg tggaagagaa gacatggata
      661 taagtgagcc tgaagagaag ctccaggaaa cagttgaacc gacttcaaag cagttaactg
      721 aatcctctga agaggcaaaa gaagcagcaa taccaggact gaatgaagat gaggtcgctt
      781 ctgggaagac agagcaggaa tcattgtgta ctgagaaagg aaaatcaatt tcaggagctt
      841 atgttcaaaa taaagaattc agagaaacag tggaggaggg agaggaaata ataagcttag
      901 agaaaaagcc aaaagaaact tcagaagatc agcctatcag ggctgcagaa aagcagggca
      961 ctttaatgaa ggtggtagaa atagaagctg aaatagaccc tcaagtcaag tcagcggatg
     1021 tgggagggga ggagccaaaa gatcaggtag ctacctctga gagtgaacta ggtaaggctg
     1081 ttcttatgga actgtcaggg caagatgttg aagcatcacc agtcgtggct gcagaggccg
     1141 gagctgaagt ctctgagaag ccagggcagg agattacagt tattcccaac aatggtccag
     1201 ttgttggaca atcaactgta ggagatcaga ctcctagtga accacagact tctgcagaaa
     1261 gactgacaga aactaaagat ggctcaagtg tagaggaggt caaggcagag ctggttcctg
     1321 aacaggagga agctatgcta cctgtagaag agtctgaggc agctggagat ggggttgaga
     1381 ccaaggtagc ccagagggcc acggagaaag cacctgaaga caaatttaag atagctgcta
     1441 atgaagagac acaagagaga gacgaacaga tgaaagaggg tgaagaaact gaaggctcag
     1501 aggaagagga cagagaaaat gacaaggctg aagaaacacc aaatgaatca gttcttgaaa
     1561 aaaagtctct tcaagaaaat gaagaggagg agatagggaa tttggaactt gcctgggata
     1621 tgctggattt agccaagatc atttttaaga ggcaagaaac aaaagaagcc cagctttatg
     1681 ctgcacaagc ccatcttaaa cttggagaag ttagtgttga atctgagaat tatatccaag
     1741 ctgtggagga gtttcaggct tgcctaagcc tgcaagaaca atatttggaa gctcacgatc
     1801 gtctccttgc agagactcac taccaattag gtttggccta tggttacaac tctcagtatg
     1861 atgaagcagt agcacagttt ggcaaatcta tcgatgtcat tgaaaagaga atggctgtac
     1921 tccatgagca gatgaaggag gccgaaggat cgtttactga atatgagaaa gaaattgagg
     1981 agctgaaaga actgctacct gaaattcgag agaagataga agacgcaaag gaatctcagc
     2041 ggagtgggaa tgtagccgaa ctggcactga aagctactct ggtggagagc tctacttccg
     2101 gtttcactcc tagtggagct ggtgcctcag tatccatgat tgccagtaga aaaccaacag
     2161 atggcgcttc ctcatcaaat tgtgtgactg atatttccca tcttgtcaga aagaagagga
     2221 aaccagagga agagagtccc cggaaggatg atgcaaagaa agctaaacaa gagccagagg
     2281 tgaatggagg cagtggggat gctgtctcca gtggaaagga agtttcagaa aacatggagg
     2341 ccgaggctga gaatcaggct gagagccaaa cagcagaggg gaccgtggag tctgcagcta
     2401 caattaaaag cactgcatgt taagaggcga gcagaacctt cctcctgagg gaaagtggtt
     2461 ttgtacataa tgtatttttt cacttttggg gattcttttt gtataactcc aataaagatt
     2521 gtaggcaaca gctgaggctt tgatggcttt ttttcttaaa tattggtgga atgctttggg
     2581 gcactctaca tttcttgtgt gtacatgttt gtgttttgct attgcacaac ccacttcctg
     2641 tctaatgtgg aggtgatcaa gtgtggctag aagcccttca tcttctactg gtgctatgtt
     2701 ctatttccca atacttggct gaaatcaatt actgtgtaag ctttcagtgg tgctgtcctt
     2761 ggatggggtc tgctaaataa gaactggtag aaatcagttt ggtgctgaaa atggatgatg
     2821 gaatatgacc caggtcttgg tttgtcccta tctgctgttc atctggtttc catttaaggc
     2881 cataatccta tcctgggaaa actggtagaa acctgggaaa ttgttcctga ggaaaacagt
     2941 tgttagactc aggctcacac tgtagtataa gacactgggg ggagaaagtg tgttattttc
     3001 tgtatagaaa ggttactata tgtcaataag tggaattgtt atattgtctg cagttttgtc
     3061 tataaaaata aatatgctaa ttttaac
//
