LOCUS       NM_153591               3744 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus asparaginyl-tRNA synthetase 2
            (mitochondrial)(putative) (Nars2), mRNA.
ACCESSION   NM_153591
VERSION     NM_153591.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3744)
  AUTHORS   Cai,Y., Yoneda,M., Tomita,T., Kurotani,R., Okamoto,M., Kido,T.,
            Abe,H., Mitzner,W., Guha,A. and Kimura,S.
  TITLE     Transgenically-expressed secretoglobin 3A2 accelerates resolution
            of bleomycin-induced pulmonary fibrosis in mice
  JOURNAL   BMC Pulm Med 15, 72 (2015)
   PUBMED   26178733
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3744)
  AUTHORS   Simon,M., Richard,E.M., Wang,X., Shahzad,M., Huang,V.H.,
            Qaiser,T.A., Potluri,P., Mahl,S.E., Davila,A., Nazli,S.,
            Hancock,S., Yu,M., Gargus,J., Chang,R., Al-Sheqaih,N., Newman,W.G.,
            Abdenur,J., Starr,A., Hegde,R., Dorn,T., Busch,A., Park,E., Wu,J.,
            Schwenzer,H., Flierl,A., Florentz,C., Sissler,M., Khan,S.N., Li,R.,
            Guan,M.X., Friedman,T.B., Wu,D.K., Procaccio,V., Riazuddin,S.,
            Wallace,D.C., Ahmed,Z.M., Huang,T. and Riazuddin,S.
  TITLE     Mutations of human NARS2, encoding the mitochondrial
            asparaginyl-tRNA synthetase, cause nonsyndromic deafness and Leigh
            syndrome
  JOURNAL   PLoS Genet 11 (3), e1005097 (2015)
   PUBMED   25807530
  REMARK    GeneRIF: In these cells we show that a decrease in oxygen
            consumption rates (OCR) and electron transport chain (ETC) activity
            can be rescued by overexpression of wild type NARS2
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162370.4 and AC074047.6.
            
            On Dec 22, 2021 this sequence version replaced NM_153591.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046799.1, AK046801.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-403               AC162370.4         71725-72127         c
            404-513             AC162370.4         67616-67725         c
            514-634             AC162370.4         65474-65594         c
            635-775             AC162370.4         63858-63998         c
            776-856             AC162370.4         50107-50187         c
            857-951             AC162370.4         20777-20871         c
            952-1002            AC162370.4         1-51                c
            1003-1084           AC074047.6         92104-92185         c
            1085-1183           AC074047.6         84418-84516         c
            1184-1221           AC074047.6         84271-84308         c
            1222-1288           AC074047.6         80557-80623         c
            1289-1426           AC074047.6         75818-75955         c
            1427-1524           AC074047.6         58309-58406         c
            1525-1551           AC074047.6         56042-56068         c
            1552-3744           AC074047.6         51093-53285         c
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.13 cM"
     gene            1..3744
                     /gene="Nars2"
                     /note="asparaginyl-tRNA synthetase 2
                     (mitochondrial)(putative)"
                     /db_xref="GeneID:244141"
                     /db_xref="MGI:MGI:2142075"
     exon            1..403
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..1696
                     /gene="Nars2"
                     /EC_number="6.1.1.22"
                     /note="probable asparaginyl-tRNA synthetase,
                     mitochondrial; asnRS; asparagine--tRNA ligase; probable
                     asparagine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="asparaginyl-tRNA synthetase precursor"
                     /protein_id="NP_705819.3"
                     /db_xref="CCDS:CCDS21454.1"
                     /db_xref="GeneID:244141"
                     /db_xref="MGI:MGI:2142075"
                     /translation="MLGARRLLGALRLCSSVSCPRPRASAKMRVRDALRVQDARGECV
                     TVQGWIRSVRSQKEVLFLHVNDGSSLESLQIVADSSFDSRELTFGSSVQVQGQLVKSQ
                     SKRQNVELKAEKIEVIGDCEAKAFPIKYKERHPLEYLRQYPHLRCRTNALGSILRVRS
                     EATAAIHSYFKDNGFVHIHTPVLTSNDCEGAGELFQVEPSSKIKGPKESFFDVPAFLT
                     VSGQLHLEVMSGAFTQVFTFGPTFRAENSQSRRHLAEFYMVEAEISFVESLQDLMQVM
                     EELFKATTEMVLSHCPEDVELCHQFIAAGQKGRLEHMLKNNFLIISYTEAIEILKQAS
                     QNFAFTPKWGVDLQTEHEKYLVRHCGNIPVFVINYPSELKPFYMRENEDGPQNTVAAV
                     DLLVPGVGELFGGSLREERYHVLEQRLARSGLTKAYQWYLDLRKFGSVPHGGFGMGFE
                     RYLQCILGVDNIKDVIPFPRFTHSCLL"
     exon            404..513
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            514..634
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..775
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            776..856
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            857..951
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1084
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1183
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1221
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1288
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1426
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1524
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1551
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..3744
                     /gene="Nars2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2453..2458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nars2"
                     /note="hexamer: ATTAAA"
     polyA_site      2479
                     /gene="Nars2"
                     /note="major polyA site"
     regulatory      2967..2972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nars2"
                     /note="hexamer: TATAAA"
     polyA_site      2991
                     /gene="Nars2"
     regulatory      3714..3719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nars2"
                     /note="hexamer: ATTAAA"
     polyA_site      3744
                     /gene="Nars2"
ORIGIN      
        1 agttgcaacg tgctttaagt ctgggtctgc actccggtcc catgtacggt gtgacactga
       61 ccgctgtgtt ccgtgtagtc gaaccttctg tgctttcggg agcgggacgc ggaggtgggg
      121 tcccgtggga actgctcggg gcgcggaggg aagagcgaca ggagcgcatg acggcaggac
      181 tgcgcggctg aaaaaggctg ctctcgggga ctgtgagccg ggacgtagcc cggcatcccg
      241 tggaaccctg tccggtctcg ccatgctcgg ggcccggcgc ctgctcggcg cgctgcgcct
      301 ctgttcctcc gtttcctgcc ccaggccccg cgcgtctgcc aaaatgcgcg tgcgggacgc
      361 cctcagggtc caggacgcga gaggggagtg cgtaacggtg cagggatgga tccggtccgt
      421 cagatcccag aaggaagttt tgttcctgca tgtaaatgac ggatcttctt tggaaagcct
      481 tcagattgtt gcggattcaa gcttcgacag tagagaattg acttttggga gttctgtgca
      541 agtccaaggg cagctggtca aaagtcagtc caagaggcaa aatgtggaac tgaaggcaga
      601 aaagattgag gttattggag actgtgaagc caaggcattc cctataaagt ataaggagcg
      661 acaccctctg gagtacctga gacagtatcc tcacctgagg tgcaggacta atgctctggg
      721 ttctatattg agggtccgca gtgaagccac agctgctatt cattcctact ttaaggacaa
      781 tggctttgtg cacattcata ccccagtact cacatccaac gactgtgagg gggccggaga
      841 gctttttcaa gtggagcctt caagcaaaat aaagggaccc aaggagagtt tctttgatgt
      901 tcctgctttc ttaactgtct caggacaact tcacctagaa gtgatgtcag gagcttttac
      961 ccaagtattt acctttggtc caacattccg agctgagaat tctcagagcc ggagacacct
     1021 ggctgaattt tatatggttg aagcggagat ttcttttgtt gaaagccttc aggatctcat
     1081 gcaggtcatg gaggaactct tcaaggcgac cacggagatg gttctctcac actgtcctga
     1141 agatgttgaa ttgtgtcacc agttcatagc tgctggccag aagggcagac tagaacatat
     1201 gctaaaaaac aacttcctaa tcatttctta tacggaggca attgagatct taaagcaagc
     1261 atcccagaac ttcgccttta cacccaagtg gggtgttgat ctacaaacgg aacatgagaa
     1321 gtacctggtg agacactgtg gcaacatacc agtctttgtc attaattatc catcagaact
     1381 caagcccttc tacatgaggg agaatgaaga tggtcctcag aatacagttg ctgctgtaga
     1441 tcttctggtt cctggagttg gggagctctt tggaggtagc ctcagggagg aacggtacca
     1501 cgtcctagag cagcgactag caagatcagg acttacgaaa gcctaccagt ggtacctgga
     1561 tcttcgcaag tttggatctg tgccccatgg aggcttcggg atgggatttg aacgctactt
     1621 acagtgcatc ctaggagttg acaatatcaa agatgttatc ccttttccaa ggtttactca
     1681 ttcgtgtctt ttgtagctga aagactgttt aatggaaagt gctccatcac ctcatctaca
     1741 catgagtatg tgtagaaaag actgggtgtt tgcagtctgg aaatgtggat ttcagtgcac
     1801 aattcttgtg ctttgagatg tactatatga aaaatagaaa tggtcttgat catcttaagt
     1861 caaaggcttt tcatggacag ataaactccc ccaaggagaa atactaagag caagtggact
     1921 ttcaaatctg ttacctcaat taggactggg catgttgtcc agctagtgga gaagccggtt
     1981 caggagagta acttgagccc ggataacatc tcaagaaaga ggaaaggaag atatggaagt
     2041 gtaggatctc agcgttcctc agggactctg acttgatagc catgtctcac cctgctttgc
     2101 ctttcattgg tagctcattt ccccttgttg tttgtttttt aacaactagt gactaaaaat
     2161 aaggtgatgt tgaccctttt cttccttgcc atttaaactt acatgacacc aatcttattc
     2221 tctgtgcttt actcaacttt atagaagaaa atattccttt tcagagtcta aattgactaa
     2281 tgaagtggct attctgaatt gaaccattca tcattgggtg gtgaaaaaaa atcacattta
     2341 ataactgttg atctttagtt ttaaaagtta aagagctaaa attaaccatt taaacttgtc
     2401 aatcattgtt ctagttcatt tatattagat gtttgtaaat attctaatag tcattaaaat
     2461 tcttttaagt taaaacctat gaaagaagtt atttttaaat tcctcttgat cctttcccag
     2521 tggaatttgg aagaaaggta tctttaatgg aaggtggtgt tggaaatgtg gccaaaccta
     2581 cgcaatgggt aattttagaa tatggtgtgg cctgatagaa tgtgaccagt gccccttaga
     2641 gtgctcaaga cctgacattc cagcaccttg gacaaaagca attactttgt ataaactaaa
     2701 acatcatttc cacagaggtc ttacagcgcc gggacacgcc tgtatttgcg tgtgtattta
     2761 aaaccacact tgaactagaa atgcagttca gaggtaagag catttggcta gcacacttgg
     2821 ggctctgggt ttgatcctta tgtgggtagg aggggggtgt cacactttta aaaactcttg
     2881 acttgtttta gtctttttac caggcttcat ttatggagaa ctattgttgg ttgtttattc
     2941 agttgaattt atatgtatca aataattata aaatgtcatg catacctaaa ataattttca
     3001 aagtttcatt agatactctt gcaggttaat tactttttaa tttaatttta tgtatctggt
     3061 tgttttctct acatacatgt atgtacacca gttacatgcc tggcaccacc cacagagact
     3121 gaaagggggc atccgatccc ctggaatttg tagttagaga tggttgtgag ctgctgtgtg
     3181 tttgctggga atagaacctg gctcctctgg aagaccagcc agtcctctta actgctgaac
     3241 catctcccca gccctcttag gttaattctg tatagccctg actgtcctgg aactttctaa
     3301 gtagaccaaa ctgcccttga cctcagttgg gcacaaccag gcctggccct ccctttggtt
     3361 agtttttaac cagtttaggt tgtgtttctt agcctaattc aaagttaaca tctgaagagt
     3421 aaacttcaga acctcagggt taaaagacac ctaagaggtt gactgggttg tcctgcctaa
     3481 tgcagagagc acctcctgcc tgggccatgt gtttggggat tttttggttg ttgttattgt
     3541 tttttgtttg aaaagatttt tttcttgaaa agattttcat tccactgctg aatctttata
     3601 atcaggataa ttgctaaggt aaagtcccag ccccagtcta ctttcccttg catgctaagt
     3661 ttagggacca atgagaggga taatgggctt tcatcggact cactgcagcc gtgattaaag
     3721 aagtccattt gtgtattcaa agaa
//
