LOCUS       NM_001377022            2594 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus asparaginyl-tRNA synthetase 1 (Nars1), transcript
            variant 4, mRNA.
ACCESSION   NM_001377022
VERSION     NM_001377022.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Beijer,D., Marte,S., Li,J.C., De Ridder,W., Chen,J.Z.,
            Tadenev,A.L.D., Miers,K.E., Deconinck,T., Macdonell,R., Marques,W.
            Jr., De Jonghe,P., Pratt,S.L., Meyer-Schuman,R., Zuchner,S.,
            Antonellis,A., Burgess,R.W. and Baets,J.
  TITLE     Dominant NARS1 mutations causing axonal Charcot-Marie-Tooth disease
            expand NARS1-associated diseases
  JOURNAL   Brain Commun 6 (2), fcae070 (2024)
   PUBMED   38495304
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2594)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2594)
  AUTHORS   Guan,N., Kobayashi,H., Ishii,K., Davidoff,O., Sha,F., Ikizler,T.A.,
            Hao,C.M., Chandel,N.S. and Haase,V.H.
  TITLE     Disruption of mitochondrial complex III in cap mesenchyme but not
            in ureteric progenitors results in defective nephrogenesis
            associated with amino acid deficiency
  JOURNAL   Kidney Int 102 (1), 108-120 (2022)
   PUBMED   35341793
REFERENCE   5  (bases 1 to 2594)
  AUTHORS   Laguesse,S., Creppe,C., Nedialkova,D.D., Prevot,P.P., Borgs,L.,
            Huysseune,S., Franco,B., Duysens,G., Krusy,N., Lee,G., Thelen,N.,
            Thiry,M., Close,P., Chariot,A., Malgrange,B., Leidel,S.A.,
            Godin,J.D. and Nguyen,L.
  TITLE     A Dynamic Unfolded Protein Response Contributes to the Control of
            Cortical Neurogenesis
  JOURNAL   Dev Cell 35 (5), 553-567 (2015)
   PUBMED   26651292
REFERENCE   6  (bases 1 to 2594)
  AUTHORS   Park,S.J., Kim,S.H., Choi,H.S., Rhee,Y. and Lim,S.K.
  TITLE     Fibroblast growth factor 2-induced cytoplasmic asparaginyl-tRNA
            synthetase promotes survival of osteoblasts by regulating
            anti-apoptotic PI3K/Akt signaling
  JOURNAL   Bone 45 (5), 994-1003 (2009)
   PUBMED   19631775
  REMARK    GeneRIF: NARS is one of the important mediators of FGF2 induced
            survival signaling in osteoblasts through the activation of
            PI3K/Akt survival pathway.
REFERENCE   7  (bases 1 to 2594)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102268.11.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR2680377.1248168.1,
                                           ERR3363658.4616419.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC102268.11        53868-53967         c
            101-183             AC102268.11        52778-52860         c
            184-249             AC102268.11        49436-49501         c
            250-342             AC102268.11        49237-49329         c
            343-432             AC102268.11        49063-49152         c
            433-511             AC102268.11        47905-47983         c
            512-582             AC102268.11        46697-46767         c
            583-663             AC102268.11        45224-45304         c
            664-885             AC102268.11        42609-42830         c
            886-1085            AC102268.11        42300-42499         c
            1086-1221           AC102268.11        41790-41925         c
            1222-1335           AC102268.11        40960-41073         c
            1336-1467           AC102268.11        39196-39327         c
            1468-1599           AC102268.11        38755-38886         c
            1600-2594           AC102268.11        37107-38101         c
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.47 cM"
     gene            1..2594
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="asparaginyl-tRNA synthetase 1"
                     /db_xref="GeneID:70223"
                     /db_xref="MGI:MGI:1917473"
     exon            1..100
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1731
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /EC_number="6.1.1.22"
                     /note="isoform 4 is encoded by transcript variant 4;
                     asparaginyl-tRNA synthetase, cytoplasmic; asparagine--tRNA
                     ligase, cytoplasmic"
                     /codon_start=1
                     /product="asparagine--tRNA ligase, cytoplasmic isoform 4"
                     /protein_id="NP_001363951.1"
                     /db_xref="GeneID:70223"
                     /db_xref="MGI:MGI:1917473"
                     /translation="MVLAELYVSDREGNDATGDGTKEKPFKTGLKALMTVGKEPFPTI
                     YVDSQKENERWDVISKSQMKNIKKMWHREQMKNDSREKKEAEDNLRREKNLEEAKKII
                     IKNDPSLPEPACVKISALEGYRGQRVKVFGWVHRLRRQGKNLMFLVLRDGTGYLQCVL
                     SDDLCYNGVVLSTESSVAVYGTLNLTPKGKQAPGGHELSCDFWELVGLAPAGGADNLI
                     NEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYCEVTTPTLVQTQV
                     EGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEFT
                     HVEAECPFLTFEDLLNRLEDLVCDVVDRVLKSPVASIVYELNPNFKPPKRPFRRMNYS
                     DAIEWLKEHDVKKEDGTFYEFGDDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQRC
                     PEDPRLTESVDVLMPNVGEIVGGSMRSWDSEEILEGYKREGIDPAPYYWYTDQRKYGT
                     CPHGGYGLGLERFLSWILNRYHIRDVCLYPRFLQRCRP"
     exon            101..183
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            184..249
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            250..342
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            343..432
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            433..511
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            512..582
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            583..663
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            664..885
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1085
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1221
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1335
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1467
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1599
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..2594
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2567..2572
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="hexamer: AATAAA"
     polyA_site      2594
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="major polyA site"
ORIGIN      
        1 agtcaaggaa gccttcagtg cacgttgggg tgagcaggcg gcggtggtgc agtgtctatg
       61 tcctcggagg tgattagggg gactgcagag atggttctag cagagctgta tgtatctgac
      121 cgagaaggaa atgacgcaac cggggatgga accaaggaga aaccatttaa aacaggccta
      181 aaggctttga tgacagttgg aaaagagcca tttcccacca tttatgtgga ctcacaaaag
      241 gaaaatgaga gatgggatgt tatttctaag tcacagatga agaacattaa aaagatgtgg
      301 cacagagaac agatgaagaa cgactctagg gagaagaaag aggcagaaga taacttacga
      361 agagaaaaga acctggagga agcaaaaaaa attattatta aaaatgaccc gagcctgcca
      421 gagccggcct gtgtaaagat tagtgcatta gaaggataca gaggccagag agtgaaggtg
      481 tttggctggg tccacaggtt gcgcaggcaa ggaaagaatt tgatgttttt ggtgttgcga
      541 gatggtacgg gttatcttca gtgtgtcttg tcagatgact tgtgttacaa tggagtagtc
      601 ctgtccaccg agagtagtgt ggcggtgtac ggaacactaa acctaactcc aaagggcaaa
      661 caggctccag gaggccatga gctgagctgt gacttctggg aactggtggg gctggccccg
      721 gctggaggag ctgataacct gatcaacgag gagtctgatg tggatgtcca gctcaacaac
      781 cggcacatga tgatccgggg agagaacatg tccaaaatcc tgaaagcgcg ctccatgatc
      841 accaggtgtt tccgggacca cttcttcgac aggggctact gtgaagttac cactccaaca
      901 ctggtgcaga cgcaggtgga aggtggggcc acgctcttca agcttgacta tttcggggaa
      961 gaagcgtttt tgacccagtc ctcacagttg tacctggaga cctgccttcc agccctggga
     1021 gatgtttttt gtatagccca gtcatacagg gccgaacagt ccaggacacg aaggcacctg
     1081 gctgagttca ctcacgtgga agctgagtgt cctttcctga ccttcgagga cctcctgaac
     1141 cgtttagagg acctagtgtg tgatgtggtg gacagagtcc tgaagtcacc agtggcaagc
     1201 atagtgtatg agctcaaccc gaactttaaa ccccccaagc ggcctttccg caggatgaac
     1261 tactcggatg ctattgagtg gctgaaggag catgatgtca agaaagaaga cgggactttc
     1321 tacgagtttg gagatgatat tcccgaagcg cctgagagac tgatgacaga caccattaat
     1381 gaaccaatcc tgctgtgtcg ctttcctgtg gagatcaagt ctttctatat gcagcgctgt
     1441 cctgaggatc ctcgacttac tgaatctgtg gacgtgttga tgcccaacgt tggtgagatt
     1501 gtaggtggct cgatgcgctc ctgggacagt gaggagattc tcgaaggcta taagagggaa
     1561 gggattgacc ccgctcctta ctactggtat acagatcaga gaaaatatgg cacctgtcct
     1621 catggagggt atggcttggg cttggaacga tttcttagct ggattctgaa caggtatcac
     1681 atccgagatg tgtgcctgta ccctcgattt ctccagcgct gcaggccata accatggggc
     1741 cctcaagcac gaaggaaatg aaaagttaag actgcctctg caaaagaaca aacgcccaaa
     1801 tcgtcccttg acttcctgtt gggggtagta gctttctctt gactttccca ttatcagaaa
     1861 aagcagtagg tatcacttga acatcaagtg acattgatgc tcctttaatg aatatactca
     1921 ttacaaaatt ttgaggaaat atatgtcatg tcactcttta gtatggagat attttattgt
     1981 gctataggtt acaatcattg tattaaataa accatacatg attatacagt ttttaattat
     2041 ggtctgacat ccagtaaaat tccatctttt tgtcctttta agtgtcgctg ggattctcga
     2101 attcaagcat ctaaaagaga aattggctgt ttgacataaa agatactgga gtcaaataca
     2161 tagcccttta cattcactgt tagtcttttc cttattcata tagtgtttat aattctgagg
     2221 ggtatgagtt gacaggggaa attaatgaag gggaaatgga ttataatttg caatgaacat
     2281 gattggatgg tgggttattc aaatgattcc aaccccgtta gcctttagtt taagaacggg
     2341 gcagatgggg cttccctctt tgtaattcct gagtaacaaa agtaaatctg tttgcaaaga
     2401 ctttgcacct gaatcttagc caccgtagac agctacgtca tccctgcctg gtccttgggt
     2461 gccatctgca ctgccttgcc gactcagcag ggctaccagt ggctgaagtg cagttgctct
     2521 ccccagatgc atgccgtgtg ttctaatggg ctgtatccca ctaaccaata aatgagcagt
     2581 ggttaaaaaa caaa
//
