LOCUS       NM_001142950            2600 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus asparaginyl-tRNA synthetase 1 (Nars1), transcript
            variant 1, mRNA.
ACCESSION   NM_001142950
VERSION     NM_001142950.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Beijer,D., Marte,S., Li,J.C., De Ridder,W., Chen,J.Z.,
            Tadenev,A.L.D., Miers,K.E., Deconinck,T., Macdonell,R., Marques,W.
            Jr., De Jonghe,P., Pratt,S.L., Meyer-Schuman,R., Zuchner,S.,
            Antonellis,A., Burgess,R.W. and Baets,J.
  TITLE     Dominant NARS1 mutations causing axonal Charcot-Marie-Tooth disease
            expand NARS1-associated diseases
  JOURNAL   Brain Commun 6 (2), fcae070 (2024)
   PUBMED   38495304
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2600)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2600)
  AUTHORS   Guan,N., Kobayashi,H., Ishii,K., Davidoff,O., Sha,F., Ikizler,T.A.,
            Hao,C.M., Chandel,N.S. and Haase,V.H.
  TITLE     Disruption of mitochondrial complex III in cap mesenchyme but not
            in ureteric progenitors results in defective nephrogenesis
            associated with amino acid deficiency
  JOURNAL   Kidney Int 102 (1), 108-120 (2022)
   PUBMED   35341793
REFERENCE   5  (bases 1 to 2600)
  AUTHORS   Laguesse,S., Creppe,C., Nedialkova,D.D., Prevot,P.P., Borgs,L.,
            Huysseune,S., Franco,B., Duysens,G., Krusy,N., Lee,G., Thelen,N.,
            Thiry,M., Close,P., Chariot,A., Malgrange,B., Leidel,S.A.,
            Godin,J.D. and Nguyen,L.
  TITLE     A Dynamic Unfolded Protein Response Contributes to the Control of
            Cortical Neurogenesis
  JOURNAL   Dev Cell 35 (5), 553-567 (2015)
   PUBMED   26651292
REFERENCE   6  (bases 1 to 2600)
  AUTHORS   Park,S.J., Kim,S.H., Choi,H.S., Rhee,Y. and Lim,S.K.
  TITLE     Fibroblast growth factor 2-induced cytoplasmic asparaginyl-tRNA
            synthetase promotes survival of osteoblasts by regulating
            anti-apoptotic PI3K/Akt signaling
  JOURNAL   Bone 45 (5), 994-1003 (2009)
   PUBMED   19631775
  REMARK    GeneRIF: NARS is one of the important mediators of FGF2 induced
            survival signaling in osteoblasts through the activation of
            PI3K/Akt survival pathway.
REFERENCE   7  (bases 1 to 2600)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102268.11.
            
            On Dec 6, 2019 this sequence version replaced NM_001142950.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052849.1, AK172657.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC102268.11        53868-53967         c
            101-183             AC102268.11        52778-52860         c
            184-249             AC102268.11        49436-49501         c
            250-342             AC102268.11        49237-49329         c
            343-432             AC102268.11        49063-49152         c
            433-511             AC102268.11        47905-47983         c
            512-582             AC102268.11        46697-46767         c
            583-669             AC102268.11        45224-45310         c
            670-891             AC102268.11        42609-42830         c
            892-1091            AC102268.11        42300-42499         c
            1092-1227           AC102268.11        41790-41925         c
            1228-1341           AC102268.11        40960-41073         c
            1342-1473           AC102268.11        39196-39327         c
            1474-1605           AC102268.11        38755-38886         c
            1606-2600           AC102268.11        37107-38101         c
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.47 cM"
     gene            1..2600
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="asparaginyl-tRNA synthetase 1"
                     /db_xref="GeneID:70223"
                     /db_xref="MGI:MGI:1917473"
     exon            1..100
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1737
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /EC_number="6.1.1.22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     asparaginyl-tRNA synthetase, cytoplasmic; asparagine--tRNA
                     ligase, cytoplasmic"
                     /codon_start=1
                     /product="asparagine--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_001136422.2"
                     /db_xref="CCDS:CCDS50308.1"
                     /db_xref="GeneID:70223"
                     /db_xref="MGI:MGI:1917473"
                     /translation="MVLAELYVSDREGNDATGDGTKEKPFKTGLKALMTVGKEPFPTI
                     YVDSQKENERWDVISKSQMKNIKKMWHREQMKNDSREKKEAEDNLRREKNLEEAKKII
                     IKNDPSLPEPACVKISALEGYRGQRVKVFGWVHRLRRQGKNLMFLVLRDGTGYLQCVL
                     SDDLCQCYNGVVLSTESSVAVYGTLNLTPKGKQAPGGHELSCDFWELVGLAPAGGADN
                     LINEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYCEVTTPTLVQT
                     QVEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAE
                     FTHVEAECPFLTFEDLLNRLEDLVCDVVDRVLKSPVASIVYELNPNFKPPKRPFRRMN
                     YSDAIEWLKEHDVKKEDGTFYEFGDDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQ
                     RCPEDPRLTESVDVLMPNVGEIVGGSMRSWDSEEILEGYKREGIDPAPYYWYTDQRKY
                     GTCPHGGYGLGLERFLSWILNRYHIRDVCLYPRFLQRCRP"
     exon            101..183
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            184..249
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            250..342
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            343..432
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            433..511
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            512..582
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            583..669
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            670..891
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1091
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1227
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1341
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1473
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1605
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..2600
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2573..2578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="hexamer: AATAAA"
     polyA_site      2600
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /note="major polyA site"
ORIGIN      
        1 agtcaaggaa gccttcagtg cacgttgggg tgagcaggcg gcggtggtgc agtgtctatg
       61 tcctcggagg tgattagggg gactgcagag atggttctag cagagctgta tgtatctgac
      121 cgagaaggaa atgacgcaac cggggatgga accaaggaga aaccatttaa aacaggccta
      181 aaggctttga tgacagttgg aaaagagcca tttcccacca tttatgtgga ctcacaaaag
      241 gaaaatgaga gatgggatgt tatttctaag tcacagatga agaacattaa aaagatgtgg
      301 cacagagaac agatgaagaa cgactctagg gagaagaaag aggcagaaga taacttacga
      361 agagaaaaga acctggagga agcaaaaaaa attattatta aaaatgaccc gagcctgcca
      421 gagccggcct gtgtaaagat tagtgcatta gaaggataca gaggccagag agtgaaggtg
      481 tttggctggg tccacaggtt gcgcaggcaa ggaaagaatt tgatgttttt ggtgttgcga
      541 gatggtacgg gttatcttca gtgtgtcttg tcagatgact tgtgtcagtg ttacaatgga
      601 gtagtcctgt ccaccgagag tagtgtggcg gtgtacggaa cactaaacct aactccaaag
      661 ggcaaacagg ctccaggagg ccatgagctg agctgtgact tctgggaact ggtggggctg
      721 gccccggctg gaggagctga taacctgatc aacgaggagt ctgatgtgga tgtccagctc
      781 aacaaccggc acatgatgat ccggggagag aacatgtcca aaatcctgaa agcgcgctcc
      841 atgatcacca ggtgtttccg ggaccacttc ttcgacaggg gctactgtga agttaccact
      901 ccaacactgg tgcagacgca ggtggaaggt ggggccacgc tcttcaagct tgactatttc
      961 ggggaagaag cgtttttgac ccagtcctca cagttgtacc tggagacctg ccttccagcc
     1021 ctgggagatg ttttttgtat agcccagtca tacagggccg aacagtccag gacacgaagg
     1081 cacctggctg agttcactca cgtggaagct gagtgtcctt tcctgacctt cgaggacctc
     1141 ctgaaccgtt tagaggacct agtgtgtgat gtggtggaca gagtcctgaa gtcaccagtg
     1201 gcaagcatag tgtatgagct caacccgaac tttaaacccc ccaagcggcc tttccgcagg
     1261 atgaactact cggatgctat tgagtggctg aaggagcatg atgtcaagaa agaagacggg
     1321 actttctacg agtttggaga tgatattccc gaagcgcctg agagactgat gacagacacc
     1381 attaatgaac caatcctgct gtgtcgcttt cctgtggaga tcaagtcttt ctatatgcag
     1441 cgctgtcctg aggatcctcg acttactgaa tctgtggacg tgttgatgcc caacgttggt
     1501 gagattgtag gtggctcgat gcgctcctgg gacagtgagg agattctcga aggctataag
     1561 agggaaggga ttgaccccgc tccttactac tggtatacag atcagagaaa atatggcacc
     1621 tgtcctcatg gagggtatgg cttgggcttg gaacgatttc ttagctggat tctgaacagg
     1681 tatcacatcc gagatgtgtg cctgtaccct cgatttctcc agcgctgcag gccataacca
     1741 tggggccctc aagcacgaag gaaatgaaaa gttaagactg cctctgcaaa agaacaaacg
     1801 cccaaatcgt cccttgactt cctgttgggg gtagtagctt tctcttgact ttcccattat
     1861 cagaaaaagc agtaggtatc acttgaacat caagtgacat tgatgctcct ttaatgaata
     1921 tactcattac aaaattttga ggaaatatat gtcatgtcac tctttagtat ggagatattt
     1981 tattgtgcta taggttacaa tcattgtatt aaataaacca tacatgatta tacagttttt
     2041 aattatggtc tgacatccag taaaattcca tctttttgtc cttttaagtg tcgctgggat
     2101 tctcgaattc aagcatctaa aagagaaatt ggctgtttga cataaaagat actggagtca
     2161 aatacatagc cctttacatt cactgttagt cttttcctta ttcatatagt gtttataatt
     2221 ctgaggggta tgagttgaca ggggaaatta atgaagggga aatggattat aatttgcaat
     2281 gaacatgatt ggatggtggg ttattcaaat gattccaacc ccgttagcct ttagtttaag
     2341 aacggggcag atggggcttc cctctttgta attcctgagt aacaaaagta aatctgtttg
     2401 caaagacttt gcacctgaat cttagccacc gtagacagct acgtcatccc tgcctggtcc
     2461 ttgggtgcca tctgcactgc cttgccgact cagcagggct accagtggct gaagtgcagt
     2521 tgctctcccc agatgcatgc cgtgtgttct aatgggctgt atcccactaa ccaataaatg
     2581 agcagtggtt aaaaaacaaa
//
