LOCUS       NP_001136422             548 aa            linear   ROD 04-FEB-2025
DEFINITION  asparagine--tRNA ligase, cytoplasmic isoform 1 [Mus musculus].
ACCESSION   NP_001136422
VERSION     NP_001136422.2
DBSOURCE    REFSEQ: accession NM_001142950.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 548)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 548)
  AUTHORS   Beijer,D., Marte,S., Li,J.C., De Ridder,W., Chen,J.Z.,
            Tadenev,A.L.D., Miers,K.E., Deconinck,T., Macdonell,R., Marques,W.
            Jr., De Jonghe,P., Pratt,S.L., Meyer-Schuman,R., Zuchner,S.,
            Antonellis,A., Burgess,R.W. and Baets,J.
  TITLE     Dominant NARS1 mutations causing axonal Charcot-Marie-Tooth disease
            expand NARS1-associated diseases
  JOURNAL   Brain Commun 6 (2), fcae070 (2024)
   PUBMED   38495304
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 548)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 548)
  AUTHORS   Guan,N., Kobayashi,H., Ishii,K., Davidoff,O., Sha,F., Ikizler,T.A.,
            Hao,C.M., Chandel,N.S. and Haase,V.H.
  TITLE     Disruption of mitochondrial complex III in cap mesenchyme but not
            in ureteric progenitors results in defective nephrogenesis
            associated with amino acid deficiency
  JOURNAL   Kidney Int 102 (1), 108-120 (2022)
   PUBMED   35341793
REFERENCE   5  (residues 1 to 548)
  AUTHORS   Laguesse,S., Creppe,C., Nedialkova,D.D., Prevot,P.P., Borgs,L.,
            Huysseune,S., Franco,B., Duysens,G., Krusy,N., Lee,G., Thelen,N.,
            Thiry,M., Close,P., Chariot,A., Malgrange,B., Leidel,S.A.,
            Godin,J.D. and Nguyen,L.
  TITLE     A Dynamic Unfolded Protein Response Contributes to the Control of
            Cortical Neurogenesis
  JOURNAL   Dev Cell 35 (5), 553-567 (2015)
   PUBMED   26651292
REFERENCE   6  (residues 1 to 548)
  AUTHORS   Park,S.J., Kim,S.H., Choi,H.S., Rhee,Y. and Lim,S.K.
  TITLE     Fibroblast growth factor 2-induced cytoplasmic asparaginyl-tRNA
            synthetase promotes survival of osteoblasts by regulating
            anti-apoptotic PI3K/Akt signaling
  JOURNAL   Bone 45 (5), 994-1003 (2009)
   PUBMED   19631775
  REMARK    GeneRIF: NARS is one of the important mediators of FGF2 induced
            survival signaling in osteoblasts through the activation of
            PI3K/Akt survival pathway.
REFERENCE   7  (residues 1 to 548)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102268.11.
            
            On Dec 6, 2019 this sequence version replaced NP_001136422.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052849.1, AK172657.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.47 cM"
     Protein         1..548
                     /product="asparagine--tRNA ligase, cytoplasmic isoform 1"
                     /EC_number="6.1.1.22"
                     /note="asparaginyl-tRNA synthetase, cytoplasmic;
                     asparagine--tRNA ligase, cytoplasmic"
                     /calculated_mol_wt=62987
     Region          127..209
                     /region_name="AsnRS_cyto_like_N"
                     /note="N-terminal, anticodon recognition domain of the
                     type found in human and Saccharomyces cerevisiae
                     cytoplasmic asparaginyl-tRNA synthetase (AsnRS), in Brugia
                     malayai AsnRs and, in various putative bacterial AsnRSs.
                     This domain is a beta-barrel domain (OB...; cd04323"
                     /db_xref="CDD:239818"
     Site            order(133,152,178,209)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239818"
     Site            order(136,138..139,146,157,159,174,188,199)
                     /site_type="active"
                     /note="putative anticodon binding site [active]"
                     /db_xref="CDD:239818"
     Region          225..544
                     /region_name="AsxRS_core"
                     /note="Asx tRNA synthetase (AspRS/AsnRS) class II core
                     domain. Assignment to class II aminoacyl-tRNA synthetases
                     (aaRS) based upon its structure and the presence of three
                     characteristic sequence motifs in the core domain. This
                     family includes AsnRS as well as...; cd00776"
                     /db_xref="CDD:238399"
     Site            order(232,234,238,243,249..250,253,257,264,267,269,
                     271..273,275,286..287,289..291,303,306,309..312,333..334,
                     343..344,349,504,507..510,541..543)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:238399"
     Site            271..275
                     /site_type="other"
                     /note="motif 1"
                     /db_xref="CDD:238399"
     Site            321..324
                     /site_type="other"
                     /note="motif 2"
                     /db_xref="CDD:238399"
     Site            order(322,324,330..331,471,474,519,522)
                     /site_type="active"
                     /db_xref="CDD:238399"
     Site            519..522
                     /site_type="other"
                     /note="motif 3"
                     /db_xref="CDD:238399"
     CDS             1..548
                     /gene="Nars1"
                     /gene_synonym="3010001M15Rik; ASNRS; Nars"
                     /coded_by="NM_001142950.2:91..1737"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS50308.1"
                     /db_xref="GeneID:70223"
                     /db_xref="MGI:MGI:1917473"
ORIGIN      
        1 mvlaelyvsd regndatgdg tkekpfktgl kalmtvgkep fptiyvdsqk enerwdvisk
       61 sqmknikkmw hreqmkndsr ekkeaednlr reknleeakk iiikndpslp epacvkisal
      121 egyrgqrvkv fgwvhrlrrq gknlmflvlr dgtgylqcvl sddlcqcyng vvlstessva
      181 vygtlnltpk gkqapgghel scdfwelvgl apaggadnli neesdvdvql nnrhmmirge
      241 nmskilkars mitrcfrdhf fdrgycevtt ptlvqtqveg gatlfkldyf geeafltqss
      301 qlyletclpa lgdvfciaqs yraeqsrtrr hlaefthvea ecpfltfedl lnrledlvcd
      361 vvdrvlkspv asivyelnpn fkppkrpfrr mnysdaiewl kehdvkkedg tfyefgddip
      421 eaperlmtdt inepillcrf pveiksfymq rcpedprlte svdvlmpnvg eivggsmrsw
      481 dseeilegyk regidpapyy wytdqrkygt cphggyglgl erflswilnr yhirdvclyp
      541 rflqrcrp
//
