LOCUS       NM_178728               3726 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus N-acyl phosphatidylethanolamine phospholipase D
            (Napepld), transcript variant 4, mRNA.
ACCESSION   NM_178728
VERSION     NM_178728.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Tevosian,M., Todorov,H., Lomazzo,E., Bindila,L., Ueda,N.,
            Bassetti,D., Warm,D., Kirischuk,S., Luhmann,H.J., Gerber,S. and
            Lutz,B.
  TITLE     NAPE-PLD deletion in stress-TRAPed neurons results in an anxiogenic
            phenotype
  JOURNAL   Transl Psychiatry 13 (1), 152 (2023)
   PUBMED   37149657
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Palese,F., Pontis,S., Realini,N. and Piomelli,D.
  TITLE     NAPE-specific phospholipase D regulates LRRK2 association with
            neuronal membranes
  JOURNAL   Adv Pharmacol 90, 217-238 (2021)
   PUBMED   33706934
  REMARK    GeneRIF: NAPE-specific phospholipase D regulates LRRK2 association
            with neuronal membranes.
REFERENCE   3  (bases 1 to 3726)
  AUTHORS   Rastelli,M., Van Hul,M., Terrasi,R., Lefort,C., Regnier,M.,
            Beiroa,D., Delzenne,N.M., Everard,A., Nogueiras,R., Luquet,S.,
            Muccioli,G.G. and Cani,P.D.
  TITLE     Intestinal NAPE-PLD contributes to short-term regulation of food
            intake via gut-to-brain axis
  JOURNAL   Am J Physiol Endocrinol Metab 319 (3), E647-E657 (2020)
   PUBMED   32776827
  REMARK    GeneRIF: Intestinal NAPE-PLD contributes to short-term regulation
            of food intake via gut-to-brain axis.
REFERENCE   4  (bases 1 to 3726)
  AUTHORS   Aggarwal,G., Zarrow,J.E., Mashhadi,Z., Flynn,C.R., Vinson,P.,
            Weaver,C.D. and Davies,S.S.
  TITLE     Symmetrically substituted dichlorophenes inhibit
            N-acyl-phosphatidylethanolamine phospholipase D
  JOURNAL   J Biol Chem 295 (21), 7289-7300 (2020)
   PUBMED   32284327
  REMARK    GeneRIF: Symmetrically substituted dichlorophenes inhibit
            N-acyl-phosphatidylethanolamine phospholipase D.
REFERENCE   5  (bases 1 to 3726)
  AUTHORS   Lefort,C., Roumain,M., Van Hul,M., Rastelli,M., Manco,R.,
            Leclercq,I., Delzenne,N.M., Marzo,V.D., Flamand,N., Luquet,S.,
            Silvestri,C., Muccioli,G.G. and Cani,P.D.
  TITLE     Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism
  JOURNAL   Cells 9 (5), 1247 (2020)
   PUBMED   32443626
  REMARK    GeneRIF: Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid
            Metabolism.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3726)
  AUTHORS   Simon,G.M. and Cravatt,B.F.
  TITLE     Endocannabinoid biosynthesis proceeding through
            glycerophospho-N-acyl ethanolamine and a role for
            alpha/beta-hydrolase 4 in this pathway
  JOURNAL   J Biol Chem 281 (36), 26465-26472 (2006)
   PUBMED   16818490
  REMARK    GeneRIF: Results support the existence of an N-acyl
            phosphatidylethanolamine (NAPE)-phospholipase D-independent route
            for N-acyl ethanolamine (NAE) biosynthesis and suggest that Abh4
            plays a role in this pathway by acting as a (lyso)NAPE-selective
            lipase.
REFERENCE   7  (bases 1 to 3726)
  AUTHORS   Wang,J., Okamoto,Y., Morishita,J., Tsuboi,K., Miyatake,A. and
            Ueda,N.
  TITLE     Functional analysis of the purified anandamide-generating
            phospholipase D as a member of the metallo-beta-lactamase family
  JOURNAL   J Biol Chem 281 (18), 12325-12335 (2006)
   PUBMED   16527816
REFERENCE   8  (bases 1 to 3726)
  AUTHORS   Leung,D., Saghatelian,A., Simon,G.M. and Cravatt,B.F.
  TITLE     Inactivation of N-acyl phosphatidylethanolamine phospholipase D
            reveals multiple mechanisms for the biosynthesis of
            endocannabinoids
  JOURNAL   Biochemistry 45 (15), 4720-4726 (2006)
   PUBMED   16605240
  REMARK    GeneRIF: Inactivation of N-acyl phosphatidylethanolamine
            phospholipase D reveals multiple mechanisms for the biosynthesis of
            endocannabinoids.
REFERENCE   9  (bases 1 to 3726)
  AUTHORS   Okamoto,Y., Morishita,J., Wang,J., Schmid,P.C., Krebsbach,R.J.,
            Schmid,H.H. and Ueda,N.
  TITLE     Mammalian cells stably overexpressing
            N-acylphosphatidylethanolamine-hydrolysing phospholipase D exhibit
            significantly decreased levels of N-acylphosphatidylethanolamines
  JOURNAL   Biochem J 389 (Pt 1), 241-247 (2005)
   PUBMED   15760304
  REMARK    GeneRIF: results confirm that overexpressed NAPE-PLD is capable of
            forming NAEs, including anandamide, in living cells
REFERENCE   10 (bases 1 to 3726)
  AUTHORS   Okamoto,Y., Morishita,J., Tsuboi,K., Tonai,T. and Ueda,N.
  TITLE     Molecular characterization of a phospholipase D generating
            anandamide and its congeners
  JOURNAL   J Biol Chem 279 (7), 5298-5305 (2004)
   PUBMED   14634025
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125343.2, BC062890.1 and AC161484.4.
            
            On Jan 20, 2018 this sequence version replaced NM_178728.5.
            
            Transcript Variant: This variant (4) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a. Variants 2, 3, and
            4 all encode the same isoform (b).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062890.1, AK080888.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC125343.2         82460-82488         c
            30-3659             BC062890.1         1-3630
            3660-3726           AC161484.4         10805-10871
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 9.97 cM"
     gene            1..3726
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="N-acyl phosphatidylethanolamine phospholipase D"
                     /db_xref="GeneID:242864"
                     /db_xref="MGI:MGI:2140885"
     exon            1..192
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            193..502
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..1399
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /EC_number="3.1.4.54"
                     /note="isoform b is encoded by transcript variant 4;
                     Metallo-beta-lactamase domain containing 1;
                     N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase
                     D; NAPE-hydrolyzing phospholipase D"
                     /codon_start=1
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D isoform b"
                     /protein_id="NP_848843.1"
                     /db_xref="CCDS:CCDS19105.1"
                     /db_xref="GeneID:242864"
                     /db_xref="MGI:MGI:2140885"
                     /translation="MDEYEDSQSPAPSYQYPKETLRKRQNSVQNSGGSVSSRFSRKSF
                     KLDYRLEEDVTKSKKGKDGRFVNPWPTWKNISIPNVLRWLIMEKNHSGVPGSKEELDK
                     ELPVLKPYFVSDPEDAGVREAGLRVTWLGHATLMVEMDELIFLTDPMFSSRASPSQYM
                     GPKRFRRPPCTISELPTIDAVLISHNHYDHLDYGSVLALNERFGSELRWFVPLGLLDW
                     MQKCGCENVIELDWWEENCVPGHDKVTFVFTPSQHWCKRTLLDDNKVLWGSWSVLGPW
                     SRFFFAGDTGYCPAFEEIGKRFGPFDLAAIPIGAYEPRWFMKYQHADPEDAVRIHIDL
                     QTKRSVAIHWGTFALANEHYLEPPVKLNEALERYGLSCEDFFILKHGESRYLNTDDRA
                     FEET"
     exon            503..1149
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1264
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..3726
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1431..1436
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="hexamer: AAGAAA"
     polyA_site      1469
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="major polyA site"
     regulatory      3637..3642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="hexamer: AATAAA"
     polyA_site      3654
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="major polyA site"
     regulatory      3703..3708
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="hexamer: AATAAA"
     polyA_site      3726
                     /gene="Napepld"
                     /gene_synonym="A530089G06; Mbldc1; NAPE-PLD"
                     /note="major polyA site"
ORIGIN      
        1 atacagcgcg ccgccttccc gggaacctga gcgcctgcgg gtgagcctcc ctgctgttag
       61 cctggatctt cgcgggaggc ggttcggcga ccgcggttcc ttttcgttgg tttgctcctt
      121 agtctcgata tctgcgtgga acagcctttc tggggctgtt cggatacctc ggtgaagtgc
      181 agcgggtttc gacttctcgc cgagggacat ggatgagtat gaggacagcc agtctccagc
      241 gccaagctat cagtatccaa aagaaacact gagaaagcgc cagaattcag tgcagaattc
      301 aggaggaagt gtgtcttcta ggttctccag gaaaagcttc aagctggatt acagactaga
      361 ggaggacgta actaaatcaa agaaaggaaa agacgggaga tttgttaacc catggccaac
      421 atggaaaaac atctccatcc cgaatgtgct cagatggctg ataatggaga agaatcacag
      481 cggcgttcca ggttccaaag aggaacttga caaagagctc ccggtgctta agccatattt
      541 tgtcagtgac cctgaagacg ctggagtgag agaggctggc ttacgagtca cgtggctggg
      601 acatgcgacg ctgatggtgg aaatggacga gctcatcttc ctcacggacc ccatgttcag
      661 ctcccgtgcc tctccctcgc agtacatggg tccgaagcgg tttcgccgcc cgccgtgtac
      721 aataagcgaa ctccccacga tagatgctgt cctcatcagt cacaaccact acgaccacct
      781 agactacggc tcggtcctgg cgttgaacga gcggttcggc agcgagctgc ggtggtttgt
      841 gcccttgggc cttcttgact ggatgcagaa atgtggctgc gagaacgtga ttgagctgga
      901 ctggtgggag gagaactgcg tccctggcca cgacaaggtc accttcgtct tcacgccttc
      961 ccagcactgg tgcaaaagga ccctcctgga cgacaacaag gttctctggg gcagctggtc
     1021 cgtgctaggg ccttggagtc gattcttctt tgctggggat actggctact gccccgcttt
     1081 tgaagagatt ggaaaaaggt ttggtccttt tgaccttgcg gccattccca tcggagctta
     1141 tgaaccaagg tggtttatga aataccagca tgcagaccca gaagatgctg taaggattca
     1201 cattgacctt caaacaaaga gatctgtggc gattcactgg gggacgtttg ccttagctaa
     1261 tgagcattac ctagagccgc cagtgaaact gaatgaagct ctagagagat acggactttc
     1321 ttgtgaggat ttcttcatac tgaagcatgg agagtcgaga tacttgaata ccgatgatag
     1381 agcttttgaa gaaacatgaa actaggaatt ttcagtgaca acaaaagact aagaaattca
     1441 caaatattat gacacttttc acatggaaac aatttctgca agtgtgtgtt ttgttttata
     1501 ttataacttg ccaattgata agactagctt acataaattg tgggagtcta gggatgggtt
     1561 atttaagaaa ctgtccaaaa tagactttaa aaaaccatac caatgataca aacattgcaa
     1621 aatattgttt tttatcaaaa cattatattt tagtggcagt aaaatttctg aggttgtgaa
     1681 aaactgacat taaagttcca tttctttttc tattcagggc tttctacaga gagtcatgct
     1741 tacctttcta gattacacac acacacacac acacacacac acaccacaca cacaccacac
     1801 acacacccct ttcttatatt tatgtaatga taaacttgac ctccacatgg tcattaactg
     1861 ctggtcgtgg tgatgcacac ctgcagtcca gcactccaaa gaataagcag acaggtgaca
     1921 agttcaaggc cagctagagc tacaagtgag agaagaaaac attagcttac gtgccaaatg
     1981 ttaagtgatt ttgttaacca taagaacata gttgtttggg ctaaatttat ttttttaaaa
     2041 taggtttttt ttattgtggt caaatataca tctcataaaa tgatcatttt aactatggtg
     2101 aaaagaagct ctttggcatc aactgtttgc ataggtgtgc ggccatgagc atcatgcact
     2161 tacatctctg agagggcatc atccacacag ctgcatttcc caggttcaag gctgatgggc
     2221 agagaaggca agagtgggtt tgggacttag ggcagaccct gtccaaaccc agctataact
     2281 gaagcctctc ccacaggaga ggatgtattc ctgagtggga ggacttcacg cactggggcg
     2341 gctctcactt tctacttcct ctgtactctc tcctgctccc tgccccaaga tgtcaggtag
     2401 acagatgtac tagtgagttt tacatgactt ggatgtggta tatattttta tattaaatga
     2461 cctataagca caagtgtacc ttttagtaag ccattgtgaa atgccagtag ttactctgtg
     2521 gctatctgct gcccaggctt tgtcccagta cgcctctgca ttcacaggtt tgttctgtac
     2581 cttaaggaag tgagctgcgg ggtcttagtg aacgtacact ctgcttctgc tttctagacc
     2641 aatgggacaa agcagtgagt tttctatata tatcaaatat tatataggga gtactcaata
     2701 atttgattca tttgcatggg tgatgcatgt caccttggcc tctcattgga attttgccca
     2761 tcccatcatg gacttgatta ccccaaaaca agtaactttt atgttcagac ccaatatcac
     2821 catgtacaag aggacaatgt taacctacct cccgaggcag agaataatat ttttaaagca
     2881 ctttagaaat ctaagggaga aaacttatgg tatcactctg atgcagacag gcacagtaga
     2941 cagctgaaga gcaatgctcc cgttgatgca ttgttcctgc ccagatgctg aactgggggc
     3001 tgaccacctc acccactcac cttgtgactt tagaggggaa tagccaatat gagcgaccat
     3061 cagaatgtag tatttttata taaaaggttt ccaccactca caaggagaga tgtgtactgt
     3121 aaccctgcgt gtacagtgaa tgtggctcag aggcactaat gcacaagatt ccatttaaag
     3181 tcagcttgag gaaaaccggg caagcgtcgg ctctccactg caagcggaca tgaatctgga
     3241 ccgtagggcc acgggtgcca gccttggggc acaccgctcc accgggtacc gctgcagcca
     3301 ctgtgttact gtaagccaca ctgaaacttc tcttaagcag ctgatatagc tttaatattt
     3361 ctgcatgaac ggccttggca tagcttttgt gcccacatgg acatggtcca ggcttcccat
     3421 gtgatttgtg gtctggtcct gggatgaatt gggattgcct catgtttttt tctttttctc
     3481 tttctttcag gcttcctcat ggtttttctt tgtttctgtt tacatgtaag ttccactcgt
     3541 gaagtacacc aaatctctct cacacaacca ccctattgaa tggttttcac tttatttttc
     3601 taatctacat ttcaaaggat gtatgcataa agtgaaaata aattttgaaa aaaagtctac
     3661 tcttgttagt ctaatataca ttgtaaaaat gtctatcttc taaataaagt aaatattata
     3721 aaagta
//
