LOCUS       NM_053179               1915 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Mus musculus N-acetylneuraminic acid synthase (sialic acid
            synthase) (Nans), transcript variant 1, mRNA.
ACCESSION   NM_053179
VERSION     NM_053179.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Willems,A.P., Sun,L., Schulz,M.A., Tian,W., Ashikov,A., van
            Scherpenzeel,M., Hermans,E., Clausen,H., Yang,Z. and Lefeber,D.J.
  TITLE     Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential
            for de novo sialic acid biosynthesis
  JOURNAL   Biochim Biophys Acta Gen Subj 1863 (10), 1471-1479 (2019)
   PUBMED   31121216
REFERENCE   3  (bases 1 to 1915)
  AUTHORS   Abeln,M., Albers,I., Peters-Bernard,U., Flachsig-Schulz,K.,
            Kats,E., Kispert,A., Tomlinson,S., Gerardy-Schahn,R.,
            Munster-Kuhnel,A. and Weinhold,B.
  TITLE     Sialic acid is a critical fetal defense against maternal complement
            attack
  JOURNAL   J Clin Invest 129 (1), 422-436 (2019)
   PUBMED   30382946
REFERENCE   4  (bases 1 to 1915)
  AUTHORS   Fujita,A., Sato,C. and Kitajima,K.
  TITLE     Identification of the nuclear export signals that regulate the
            intracellular localization of the mouse CMP-sialic acid synthetase
  JOURNAL   Biochem Biophys Res Commun 355 (1), 174-180 (2007)
   PUBMED   17292865
  REMARK    GeneRIF: Intracellular localization of this enzyme is regulated not
            only due to the nuclear localization signal but also two nuclear
            export signals.
REFERENCE   5  (bases 1 to 1915)
  AUTHORS   Li,X.Y., Cui,X.S. and Kim,N.H.
  TITLE     Transcription profile during maternal to zygotic transition in the
            mouse embryo
  JOURNAL   Reprod Fertil Dev 18 (6), 635-645 (2006)
   PUBMED   16930510
REFERENCE   6  (bases 1 to 1915)
  AUTHORS   Schwarzkopf,M., Knobeloch,K.P., Rohde,E., Hinderlich,S.,
            Wiechens,N., Lucka,L., Horak,I., Reutter,W. and Horstkorte,R.
  TITLE     Sialylation is essential for early development in mice
  JOURNAL   Proc Natl Acad Sci U S A 99 (8), 5267-5270 (2002)
   PUBMED   11929971
REFERENCE   7  (bases 1 to 1915)
  AUTHORS   Nakata,D., Close,B.E., Colley,K.J., Matsuda,T. and Kitajima,K.
  TITLE     Molecular cloning and expression of the mouse N-acetylneuraminic
            acid 9-phosphate synthase which does not have deaminoneuraminic
            acid (KDN) 9-phosphate synthase activity
  JOURNAL   Biochem Biophys Res Commun 273 (2), 642-648 (2000)
   PUBMED   10873658
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683884.26.
            
            On Mar 30, 2023 this sequence version replaced NM_053179.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088912.1, BC057977.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-172               AL683884.26        100513-100684
            173-388             AL683884.26        103900-104115
            389-488             AL683884.26        110237-110336
            489-643             AL683884.26        111260-111414
            644-910             AL683884.26        111907-112173
            911-1915            AL683884.26        113629-114633
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 24.83 cM"
     gene            1..1915
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /note="N-acetylneuraminic acid synthase (sialic acid
                     synthase)"
                     /db_xref="GeneID:94181"
                     /db_xref="MGI:MGI:2149820"
     exon            1..172
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1120
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /EC_number="2.5.1.57"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sialic acid synthase; N-acetylneuraminic acid phosphate
                     synthase; N-acetylneuraminic acid 9-phosphate synthetase;
                     N-acetylneuraminate-9-phosphate synthase"
                     /codon_start=1
                     /product="N-acetylneuraminate-9-phosphate synthase isoform
                     1"
                     /protein_id="NP_444409.1"
                     /db_xref="CCDS:CCDS18152.1"
                     /db_xref="GeneID:94181"
                     /db_xref="MGI:MGI:2149820"
                     /translation="MPLELELCPGRWVGGKHPCFIIAEIGQNHQGDIDVAKRMIRTAK
                     ECGADCAKFQKSELEFKFNRKALERPYTSKHSWGKTYGEHKRHLEFSHDQYKELQSYA
                     QEIGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAKKGRPMVISSGMQ
                     SMDTMKQVYQIVKPLNPNFCFLQCTSAYPLQPEDANLRVISEYQKLFPDIPIGYSGHE
                     TGIAISVAAVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSP
                     TKQLLPCEMACNEKLGKSVVAKVKIPAGTTLTLDMLTVKVGEPKGYPPEDIFNLAGKK
                     VLVTIEEDDTVMEESVESHSKKIKA"
     exon            173..388
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     exon            389..488
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     exon            489..643
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     exon            644..910
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1915
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1121..1126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /note="hexamer: AATAAA"
     polyA_site      1150
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
                     /note="major polyA site"
     polyA_site      1915
                     /gene="Nans"
                     /gene_synonym="4632418E04Rik; Sas"
ORIGIN      
        1 ggaaacgccc ggggcagcaa gcaggcgcgg gtaatgcacc atgccgctgg aactggagct
       61 gtgtcccggg cgctgggtgg gtggaaagca cccgtgcttc atcatcgcgg agatcggcca
      121 gaaccaccaa ggagacatag atgtggccaa gcgcatgatc cgcactgcca aggagtgtgg
      181 ggccgactgc gctaagtttc agaagagcga gttggagttc aagtttaacc ggaaggccct
      241 ggagagacca tatacttcga agcattcatg ggggaagacg tatggggagc acaagcggca
      301 tctggaattc agccacgacc agtacaagga gctgcagagc tatgcgcagg agatcggcat
      361 cttcttcact gcctctggca tggacgagat ggcagttgag tttctgcacg aactgaatgt
      421 tccctttttc aaagttggat ctggggacac taacaatttt ccctacctgg aaaagacagc
      481 caagaaaggt cgtcctatgg tgatctccag cgggatgcag tcaatggaca ccatgaagca
      541 agtctatcag atcgtgaagc cgctgaatcc caacttctgc ttcctccaat gcaccagcgc
      601 gtacccacta cagcccgagg atgccaacct gcgcgtcatc tcggaatacc agaagctctt
      661 tcccgacatt cccatcgggt attccgggca cgagacgggc atcgccatat ctgtggccgc
      721 cgtggctctg ggggccaagg tgttggaacg tcacataacg ttggacaaga cctggaaggg
      781 gagtgaccac tcagcctcgc tggagcctgg ggagctggca gagctggtgc ggtctgtgcg
      841 cctggtggag cgggccctgg gctccccaac caagcagctg ctgccctgtg agatggcctg
      901 caatgagaag ctcggcaagt ctgtggtagc caaagtgaaa atcccagcag gcaccaccct
      961 gaccctggac atgctcactg tgaaggtggg ggagcccaaa ggctatcctc ctgaagacat
     1021 cttcaaccta gcgggcaaaa aggtgctggt cactatcgaa gaagatgaca cggtcatgga
     1081 agaatccgtg gaaagtcaca gcaagaaaat caaggcttaa aataaaagtg ccactgcggc
     1141 ttgtcagcca ccctgctgct gctgtccagt gtgttgggtg cagaggggca ggaatgtcag
     1201 tctccaggcc accctgtcgc agtggccacc tgtgtctagc ccattgggaa ggaggagcca
     1261 acgaggctgt tgtgcttcgg agcgtccagc cagagaaggt ggcccagagc acatgcagct
     1321 cctttagggt ctctgtgtgg ctctgttgac atcttcagcc ttcaacttcc cactctacaa
     1381 agcaactcca gggacctgag ctctcagctc ctcatagagc accgaacggt gggcattaga
     1441 ctctgcctga gcccctgtgg gcagagacaa gctctcccaa ctctgtgagc gaaggcaggg
     1501 acagagcgtt cacccccttt ggcagtgtca ccgccagagg cctacggttc ttagcagcca
     1561 gtccctttct cagaggcctg tcctcagaca tcaggtgcac actaacccac ggtacagttg
     1621 gccaagcaga ggcagctgtg agaaggggaa tgggaagcag ggaggaacac gggtctgggg
     1681 ggtggggggt ggggtggagg gggtggagct tgttcatctt aaccatgggg gaggtgcaga
     1741 catcagccct gccgtaagca ccagggtctt gtcacatatg acagattaca aacccagaca
     1801 aaggtgctat tgtttgactt gtttaagggc atgaactctt accgaggctc actgtcacat
     1861 tcactttgtt ctttggaatc aatgctgaga ttaatcattt tgttcttttt aagta
//
