LOCUS       NM_173446               3142 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus NALCN channel auxiliary factor 1 (Nalf1), transcript
            variant 1, mRNA.
ACCESSION   NM_173446
VERSION     NM_173446.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Kang,Y., Wu,J.X. and Chen,L.
  TITLE     Structure of voltage-modulated sodium-selective NALCN-FAM155A
            channel complex
  JOURNAL   Nat Commun 11 (1), 6199 (2020)
   PUBMED   33273469
  REMARK    GeneRIF: Structure of voltage-modulated sodium-selective
            NALCN-FAM155A channel complex.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3142)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099760.5 and AC107823.11.
            
            On Nov 17, 2006 this sequence version replaced NM_173446.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032202.1, AK075681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164132, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-947               AC099760.5         172533-173479       c
            948-1119            AC107823.11        174887-175058       c
            1120-3142           AC107823.11        147963-149985       c
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 3.9 cM"
     gene            1..3142
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /note="NALCN channel auxiliary factor 1"
                     /db_xref="GeneID:270028"
                     /db_xref="MGI:MGI:2142765"
     exon            1..947
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..1409
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane protein 28; transmembrane protein FAM155A;
                     family with sequence similarity 155, member A"
                     /codon_start=1
                     /product="NALCN channel auxiliary factor 1 isoform 1"
                     /protein_id="NP_775622.1"
                     /db_xref="CCDS:CCDS40216.1"
                     /db_xref="GeneID:270028"
                     /db_xref="MGI:MGI:2142765"
                     /translation="MTRGAWMCRQYDDGLKIWLAAPRENEKPFIDSERAQKWRLSLAS
                     LLFFTVLLSDHLWFCAEAKLTRTRDKEHHQQQQQQQQQQQQQQQQQQQQQQRQQQRQR
                     QQQRQRQQEPSWPALLASMGESSPAAQAHRLLSASSSPTLPPSPGGGGGSKGNRGKNN
                     RSRALFLGNSAKPVWRLETCYPQGASSGQCFTVESADAVCARNWSRGAAAGEEQSSRG
                     SRPTPLWNLSDFYLSFCNSYTLWELFSGLSSPSTLNCSLDVVLTEGGEMTTCRQCIEA
                     YQDYDHHAQEKYEEFESVLHKYLQSDEYSVKSCPEDCKIVYKAWLCSQYFEVTQFNCR
                     KTIPCKQYCLEVQTRCPFILPDNDEVIYGGLSSFICTGLYETFLTNDEPECCDIRSEE
                     QTAPRPKGTVDRRDSCPRTSLTVSSATRLCPGRLKLCVLVLILLHTVLTASAAQNSTG
                     LGLGGLPTLEDNSTRED"
     exon            948..1119
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..3142
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3082..3087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
                     /note="hexamer: AATAAA"
     polyA_site      3125
                     /gene="Nalf1"
                     /gene_synonym="6430500D16; Fam155a; Tmem28"
ORIGIN      
        1 aaaatatgac caggggtgct tggatgtgtc ggcagtatga cgacggctta aaaatctggt
       61 tggcagcacc ccgggagaac gagaaaccgt tcatcgattc agagcgggct cagaaatggc
      121 gactgtctct ggcttctctc ttgtttttca cagtcctgct ctctgatcac ttgtggttct
      181 gcgccgaggc caagctgacc cggacccggg acaaagagca tcaccaacag cagcagcaac
      241 agcagcaaca gcagcagcaa cagcagcaac agcagcagca gcagcagcag cgacagcagc
      301 agcggcagcg acagcagcag aggcagcgac agcaggagcc ctcctggccc gcgctcctgg
      361 ccagcatggg ggagtcctcg cccgccgccc aggcacacag actcctctcc gcctcctcgt
      421 cccccaccct gcccccctcc ccgggaggcg gcggcggcag caagggcaac cgaggcaaga
      481 acaaccggag cagggctctt tttctaggaa actctgccaa gccggtgtgg cgcctagaga
      541 cttgttaccc ccagggcgcc tcctccggcc agtgcttcac cgtggagagc gcggacgctg
      601 tgtgcgccag gaactggagt cggggggcgg ccgcggggga ggagcagtcg tccaggggct
      661 ctcggccaac tccgctgtgg aacttgtcgg atttttacct ttcattttgt aattcctaca
      721 cactttggga gttgttttcg gggctgtcca gccccagtac tttgaactgc agtctggacg
      781 tggtgctcac ggagggcggt gagatgacca cgtgtagaca gtgcatcgag gcttaccagg
      841 actacgacca ccacgctcag gagaagtacg aagagtttga aagcgtgctg cataagtact
      901 tacagtcgga tgagtactcg gtgaagtcct gtcctgagga ctgcaagatt gtctacaaag
      961 cctggctctg ctcccagtat tttgaagtca cacagtttaa ctgcagaaag accattcctt
     1021 gcaagcaata ttgcttggag gtgcagacaa ggtgtccatt catattgccc gacaatgacg
     1081 aagtcattta cggaggcctc tccagcttca tctgcacagg gctctacgaa accttcctaa
     1141 ccaatgatga acccgaatgc tgtgacatca ggagcgagga gcaaaccgca cccagaccca
     1201 aaggaaccgt ggacagaaga gactcctgtc ccaggacatc gctcacagtg tcctcggcca
     1261 ctagactgtg ccccggccgg ctgaagctgt gtgtactcgt cctcattctc ctccacacag
     1321 tgctcacggc ctccgcagcg cagaactcca cgggactggg cctgggtggc ctccccacgc
     1381 tcgaggacaa ctccacccgg gaggactgag cgcagccagg cgcgtgcgca gagcgcaggg
     1441 ctgggcaggg acacgcgctt ggcacagagc agcagtgacc caccggggat gctcacctgc
     1501 tgcagcccgg gaactgaacc cacccgggtg ctctaccctt ggacttctcg caaggcctgt
     1561 gggtaacatt caacaagatg ggcccgatcc ccaacatgga cacagccgca gctttttgcc
     1621 gactaaaagg ctgcaagtga ctcagtttct cacaccattt tatacactgt gttttaacgt
     1681 ttggaggttt tctttgcttt cagttcggtt tgggtttatt ttccgttttt aaactttttt
     1741 ttttctgcac ttgttaatgc aggatttctt tgggggtaaa ctaagtctct ccactgtcta
     1801 tcgatttcta gtcatcgtgt gtccatcaga taattctgtc tacgtcctgt cagtttctaa
     1861 tagaggaatg accgctataa cctcacggtg tagcaaaaaa aaaacaacaa caacaacaaa
     1921 aaagaataaa aaaaacacac aaaaaaacac aaaaaagaaa acaacaaaaa ttaaaaaaaa
     1981 aaatccctaa gcctccttcc aacccatgga cgccacgccc tgcaggccct gctggccctc
     2041 gccttcttct gtccctaagc agacaacatc cgcttgcttc tgtctgtgtc tcgcatggtg
     2101 ggtatgtgtc ctgcacaagc ccgagcagag ggagccaggg gcctatcagt tcctctggat
     2161 gatccaacag agtgctcaga gtcaggcagt tcacagtttt atgtgggaaa cacaaacata
     2221 cacacacaca cacataataa aagagtgaaa cgctcccgcg gacaggtcgc tccagtaccc
     2281 atccccggtt ctttcttcct ggccaggcag acaggagcag cccatggccc actgcccgca
     2341 aggtgcgggc aagcagcccc tcttcaacac cagatgcttg actccgccga gtgatgggag
     2401 cagagaccaa gaacgcaaat ccagacttct gttcctcccc tatcatggtg atggtggagg
     2461 gattctagta tacagaatat gtaccatttt atcccagtat tgcccccagc ataaggttcc
     2521 agattcttcc aggtataaaa tgtatacagc tctgcattat cacagaggaa aatcttttta
     2581 aaacaatcaa cagatcagcc atttttacca gtgtgaggcg acctcactcc agcaagctct
     2641 aggcggctga gaacaaaaag aagtctaatc actgttcgtt tttttctttt cttttctttc
     2701 ctttgtggga acatttcacc tgctgagtgt atatgaattt gctttctaaa aaaaggcttt
     2761 tatgggttta cagtagggcc agtggaaggg agagttaata agggctgttt ttacaaaaca
     2821 aacaaaacta ataattttaa aaagacactt cacattcctt cctgttctct aactacactt
     2881 gggaagtgca cttcgggtag gtttgctgtg tggctgagag atgaaggaaa cccatagaca
     2941 aggacctcct agtgaacaaa atttagttgt taactttata gctataaaac tcccccaggc
     3001 gttcgttttc gttcagacag atctatcctc tgcatcatgc ttaaccctgc gacatgcgta
     3061 cagtatgcat attttgtttt gaataaaatg ttttgttcca gtctgttaag aatattaaaa
     3121 aataataaag gtattgctta at
//
