LOCUS       NM_144931               1808 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1),
            transcript variant 1, mRNA.
ACCESSION   NM_144931 XM_914825 XM_923421 XM_923425
VERSION     NM_144931.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Littlejohn,R., Zambrano-Carrasco,J., Zou,J., Yao,Y., Kim,I.M.,
            Zhou,J., Li,J. and Su,H.
  TITLE     Inhibition of cardiomyocyte neddylation impairs embryonic cardiac
            morphogenesis
  JOURNAL   J Mol Cell Cardiol 197, 40-44 (2024)
   PUBMED   39437885
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Ayuso-Garcia,P., Sanchez-Rueda,A., Velasco-Aviles,S.,
            Tamayo-Caro,M., Ferrer-Pinos,A., Huarte-Sebastian,C., Alvarez,V.,
            Riobello,C., Jimenez-Vega,S., Buendia,I., Canas-Martin,J.,
            Fernandez-Susavila,H., Aparicio-Rey,A., Esquinas-Roman,E.M.,
            Ponte,C.R., Guhl,R., Laville,N., Perez-Andres,E., Lavin,J.L.,
            Gonzalez-Lopez,M., Camara,N.M., Aransay,A.M., Lozano,J.J.,
            Sutherland,J.D., Barrio,R., Martinez-Chantar,M.L., Azkargorta,M.,
            Elortza,F., Soriano-Navarro,M., Matute,C., Sanchez-Gomez,M.V.,
            Bayon-Cordero,L., Perez-Samartin,A., Bravo,S.B., Kurz,T.,
            Lama-Diaz,T., Blanco,M.G., Haddad,S., Record,C.J., van
            Hasselt,P.M., Reilly,M.M., Varela-Rey,M. and Woodhoo,A.
  TITLE     Neddylation orchestrates the complex transcriptional and
            posttranscriptional program that drives Schwann cell myelination
  JOURNAL   Sci Adv 10 (15), eadm7600 (2024)
   PUBMED   38608019
REFERENCE   3  (bases 1 to 1808)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1808)
  AUTHORS   Zou,J., Wang,W., Lu,Y., Ayala,J., Dong,K., Zhou,H., Wang,J.,
            Chen,W., Weintraub,N.L., Zhou,J., Li,J. and Su,H.
  TITLE     Neddylation is required for perinatal cardiac development through
            stimulation of metabolic maturation
  JOURNAL   Cell Rep 42 (1), 112018 (2023)
   PUBMED   36662623
REFERENCE   5  (bases 1 to 1808)
  AUTHORS   Xu,C., Zhou,H., Jin,Y., Sahay,K., Robicsek,A., Liu,Y., Dong,K.,
            Zhou,J., Barrett,A., Su,H. and Chen,W.
  TITLE     Hepatic neddylation deficiency triggers fatal liver injury via
            inducing NF-kappaB-inducing kinase in mice
  JOURNAL   Nat Commun 13 (1), 7782 (2022)
   PUBMED   36526632
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1808)
  AUTHORS   Vogl,A.M., Brockmann,M.M., Giusti,S.A., Maccarrone,G.,
            Vercelli,C.A., Bauder,C.A., Richter,J.S., Roselli,F., Hafner,A.S.,
            Dedic,N., Wotjak,C.T., Vogt-Weisenhorn,D.M., Choquet,D.,
            Turck,C.W., Stein,V., Deussing,J.M. and Refojo,D.
  TITLE     Neddylation inhibition impairs spine development, destabilizes
            synapses and deteriorates cognition
  JOURNAL   Nat Neurosci 18 (2), 239-251 (2015)
   PUBMED   25581363
REFERENCE   7  (bases 1 to 1808)
  AUTHORS   Joo,Y., Ha,S., Hong,B.H., Kim,Ja., Chang,K.A., Liew,H., Kim,S.,
            Sun,W., Kim,J.H., Chong,Y.H., Suh,Y.H. and Kim,H.S.
  TITLE     Amyloid precursor protein binding protein-1 modulates cell cycle
            progression in fetal neural stem cells
  JOURNAL   PLoS One 5 (12), e14203 (2010)
   PUBMED   21151996
  REMARK    GeneRIF: APP-BP1 plays an important role in the cell cycle
            progression of fetal neural stem cells
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1808)
  AUTHORS   Ohki,Y., Funatsu,N., Konishi,N. and Chiba,T.
  TITLE     The mechanism of poly-NEDD8 chain formation in vitro
  JOURNAL   Biochem Biophys Res Commun 381 (3), 443-447 (2009)
   PUBMED   19245792
REFERENCE   9  (bases 1 to 1808)
  AUTHORS   Miao,J., Xu,F., Davis,J., Otte-Holler,I., Verbeek,M.M. and Van
            Nostrand,W.E.
  TITLE     Cerebral microvascular amyloid beta protein deposition induces
            vascular degeneration and neuroinflammation in transgenic mice
            expressing human vasculotropic mutant amyloid beta precursor
            protein
  JOURNAL   Am J Pathol 167 (2), 505-515 (2005)
   PUBMED   16049335
  REMARK    GeneRIF: Cerebral microvascular amyloid beta protein deposition
            induces vascular degeneration and neuroinflammation in transgenic
            mice expressing human vasculotropic mutant amyloid beta precursor
            protein
REFERENCE   10 (bases 1 to 1808)
  AUTHORS   Aubert,J., Dunstan,H., Chambers,I. and Smith,A.
  TITLE     Functional gene screening in embryonic stem cells implicates Wnt
            antagonism in neural differentiation
  JOURNAL   Nat Biotechnol 20 (12), 1240-1245 (2002)
   PUBMED   12447396
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC130543.3.
            
            On Aug 22, 2009 this sequence version replaced NM_144931.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027124.1, AK146202.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               AC130543.3         18757-18882
            127-230             AC130543.3         22898-23001
            231-291             AC130543.3         23278-23338
            292-322             AC130543.3         25177-25207
            323-394             AC130543.3         25945-26016
            395-474             AC130543.3         26232-26311
            475-584             AC130543.3         27005-27114
            585-694             AC130543.3         28950-29059
            695-757             AC130543.3         29756-29818
            758-821             AC130543.3         30169-30232
            822-913             AC130543.3         30656-30747
            914-973             AC130543.3         33571-33630
            974-1107            AC130543.3         33716-33849
            1108-1183           AC130543.3         35131-35206
            1184-1223           AC130543.3         35448-35487
            1224-1310           AC130543.3         36660-36746
            1311-1403           AC130543.3         37173-37265
            1404-1518           AC130543.3         39939-40053
            1519-1568           AC130543.3         40145-40194
            1569-1808           AC130543.3         42127-42366
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.04 cM"
     gene            1..1808
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /note="NEDD8 activating enzyme E1 subunit 1"
                     /db_xref="GeneID:234664"
                     /db_xref="MGI:MGI:2384561"
     exon            1..126
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..1678
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NEDD8-activating enzyme E1 regulatory subunit; amyloid
                     beta precursor protein binding protein 1, 59kDa; APP-BP1;
                     amyloid protein-binding protein 1; amyloid beta precursor
                     protein-binding protein 1, 59 kDa"
                     /codon_start=1
                     /product="NEDD8-activating enzyme E1 regulatory subunit
                     isoform 1"
                     /protein_id="NP_659180.1"
                     /db_xref="CCDS:CCDS40452.1"
                     /db_xref="GeneID:234664"
                     /db_xref="MGI:MGI:2384561"
                     /translation="MAQPGKILKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTE
                     ILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSG
                     SFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVG
                     YMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVII
                     AKYLAQWYNETNGRIPKSYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTAL
                     NTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIA
                     DSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAPESISEKELKLLCSNSAFLRV
                     VRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQV
                     EEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKI
                     ITKQFVIFNNTYIYSGMSQTSATFQL"
     exon            127..230
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            231..291
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            292..322
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            323..394
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            395..474
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            475..584
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            585..694
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            695..757
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..821
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            822..913
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            914..973
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1107
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1183
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1223
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1310
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1403
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1518
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1568
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1808
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1785..1790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /note="hexamer: AATAAA"
     polyA_site      1808
                     /gene="Nae1"
                     /gene_synonym="59kDa; Appbp1"
                     /note="major polyA site"
ORIGIN      
        1 agtgcgcctg cgtgaggccg agtgggtggc ggcctcctta ggcggctttg ctgttcgcgg
       61 gccaggcgcg gccatggcgc agccagggaa gatactcaag gagcaaaagt acgaccggca
      121 gctgaggctg tggggtgatc atggacaaga agctttggaa tctgctcatg tttgcttaat
      181 aaatgcaacg gctacaggaa ctgaaattct taaaaacttg gtgctgccag gtattggatc
      241 atttacaatt attgatggaa atctggtcag cggagaagat gctggaaaca actttttcct
      301 tcaaaaaagc agtattggca agaaccgagc tcaagctgcc atggaattct tacaagaact
      361 aaatagcgat gtctctggaa gttttgtgga agagagtcca gaaaaccttc tagacaatga
      421 tccttcattt ttctgtaggt ttactattgt ggttgcaact cagcttcttg aaagtacatt
      481 gctgcgttta gcagatgttc tctggaattc ccagatccct cttttgatct gtaggacata
      541 tggactagtt ggttatatga ggattattat aaaagaacac ccagtaatag aatctcatcc
      601 agataatgca ttggaggatc tacgactaga taagccattt cctgaactga gagaacatct
      661 tcagtcttat gacttggacc atatggaaaa aaaggaccat agccatactc catggattgt
      721 gatcatagct aaatatttag cacagtggta taatgaaaca aatggacgga ttcctaaaag
      781 ttataaagaa aaagaggact tcagagattt gataagacaa gggattttaa agaatgaaaa
      841 tggagctcca gaagacgaag agaattttga agaagccatc aaaaatgtga acacagcact
      901 aaatacaact cagatcccaa gcagtattga agatatattt aatgatgatc gatgcataaa
      961 tatcaccaaa cagacaccaa ctttttggat tctagctcgt gccttaaagg aatttgtggc
     1021 caaggagggt caaggaaatt tacctgtccg aggcacaatt cctgatatga ttgcagattc
     1081 aaacaaatac ataaaactgc agaatgttta ccgagagaaa gcaaagaaag atgctgctgc
     1141 tgtaggcaat cacgttgcca aattgcttca gtctgttggc caggcaccag agtccatttc
     1201 agagaaagaa ttaaaattgc tttgcagcaa ttctgcattt cttcgagtgg taagatgtcg
     1261 atctttagct gaagagtatg gcttggatac agtcaacaaa gatgaaatta tttctagcat
     1321 ggacaatccg gataatgaga tagtcctgta cctgatgttg cgggctgttg atcgatttca
     1381 taaacagcat ggcagatacc caggagtatc taactatcaa gttgaagaag atatagggaa
     1441 gttgaagtcc tgtcttactg gcttccttca ggaatatggg ctgtctgtaa tggtgaaaga
     1501 cgattatgtc catgaatttt gccggtacgg agctgctgag ccacacacca ttgctgcgtt
     1561 cctgggagga gctgctgctc aagaagttat caaaataatc accaaacaat tcgtaatttt
     1621 taataatact tatatttata gtggtatgtc acaaacttct gcaactttcc agttgtagaa
     1681 taagcaccat gtgtaatatg ttaatggttg gaactgtaat tgtcttcata ttgtgctttg
     1741 gtttgtaaaa atctgttaaa ggacggaagt taaattgttt ccttaataaa agtttttctc
     1801 atttgtaa
//
