LOCUS       NM_030221               2660 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus NAD synthetase 1 (Nadsyn1), transcript variant 1,
            mRNA.
ACCESSION   NM_030221 XM_993970
VERSION     NM_030221.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Szot,J.O., Cuny,H., Martin,E.M., Sheng,D.Z., Iyer,K., Portelli,S.,
            Nguyen,V., Gereis,J.M., Alankarage,D., Chitayat,D., Chong,K.,
            Wentzensen,I.M., Vincent-Delorme,C., Lermine,A., Burkitt-Wright,E.,
            Ji,W., Jeffries,L., Pais,L.S., Tan,T.Y., Pitt,J., Wise,C.A.,
            Wright,H., Andrews,I.D., Pruniski,B., Grebe,T.A.,
            Corsten-Janssen,N., Bouman,K., Poulton,C., Prakash,S., Keren,B.,
            Brown,N.J., Hunter,M.F., Heath,O., Lakhani,S.A., McDermott,J.H.,
            Ascher,D.B., Chapman,G., Bozon,K. and Dunwoodie,S.L.
  TITLE     A metabolic signature for NADSYN1-dependent congenital NAD
            deficiency disorder
  JOURNAL   J Clin Invest 134 (4), e174824 (2024)
   PUBMED   38357931
  REMARK    GeneRIF: A metabolic signature for NADSYN1-dependent congenital NAD
            deficiency disorder.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2660)
  AUTHORS   Yaku,K., Palikhe,S., Izumi,H., Yoshida,T., Hikosaka,K., Hayat,F.,
            Karim,M., Iqbal,T., Nitta,Y., Sato,A., Migaud,M.E., Ishihara,K.,
            Mori,H. and Nakagawa,T.
  TITLE     BST1 regulates nicotinamide riboside metabolism via its
            glycohydrolase and base-exchange activities
  JOURNAL   Nat Commun 12 (1), 6767 (2021)
   PUBMED   34799586
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2660)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2660)
  AUTHORS   Marin,T.L., Gongol,B., Martin,M., King,S.J., Smith,L.,
            Johnson,D.A., Subramaniam,S., Chien,S. and Shyy,J.Y.
  TITLE     Identification of AMP-activated protein kinase targets by a
            consensus sequence search of the proteome
  JOURNAL   BMC Syst Biol 9, 13 (2015)
   PUBMED   25890336
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2660)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 2660)
  AUTHORS   Hara,N., Yamada,K., Terashima,M., Osago,H., Shimoyama,M. and
            Tsuchiya,M.
  TITLE     Molecular identification of human glutamine- and ammonia-dependent
            NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine
            dependency
  JOURNAL   J Biol Chem 278 (13), 10914-10921 (2003)
   PUBMED   12547821
  REMARK    Erratum:[J Biol Chem. 2003 Oct 17;278(42):41572]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY035654.1, AK020264.1 and BQ176832.1.
            
            On Apr 22, 2015 this sequence version replaced NM_030221.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK020264.1, AK042389.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY035654.1         1-11
            12-2642             AK020264.1         2-2632
            2643-2660           BQ176832.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.32 cM"
     gene            1..2660
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /note="NAD synthetase 1"
                     /db_xref="GeneID:78914"
                     /db_xref="MGI:MGI:1926164"
     exon            1..194
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..2287
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /EC_number="6.3.5.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glutamine-dependent NAD(+) synthetase; NAD(+) synthase;
                     NH3-dependent NAD(+) synthetase-like protein"
                     /codon_start=1
                     /product="glutamine-dependent NAD(+) synthetase isoform 1"
                     /protein_id="NP_084497.1"
                     /db_xref="CCDS:CCDS22047.1"
                     /db_xref="GeneID:78914"
                     /db_xref="MGI:MGI:1926164"
                     /translation="MGRKVTVATCALNQWALDFEGNFQRILKSIQIAKGKGARYRLGP
                     ELEICGYGCWDHYHESDTLLHSLQVLAALLDSPVTQDIICDVGMPIMHRNVRYNCRVI
                     FLNRKILLIRPKMALANEGNYRELRWFTPWTRSRQTEEYVLPRMLQDLTKQKTVPFGD
                     VVLATQDTCVGSEICEELWTPRSPHIDMGLDGVEIITNASGSHHVLRKAHTRVDLVTM
                     ATSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSIFAQGTQFSLDDVEVLTATLDLE
                     DVRSYKAEISSRNLEATRVSPYPRVTVDFALSVSEDLLEPVSEPMEWTYHRPEEEISL
                     GPACWLWDFLRRSKQAGFFLPLSGGVDSAASACIVYSMCCLVCDAVKSGNQQVLTDVQ
                     NLVDESSYTPQDPRELCGRLLTTCYMASENSSQETHSRATKLAQLIGSYHINLSIDTA
                     VKAVLGIFSLMTGKLPRFSAHGGSSRENLALQNVQARIRMVLAYLFAQLSLWSRGARG
                     SLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCAERFQLPVLQTI
                     LSAPATAELEPLADGQVSQMDEEDMGMTYAELSIFGRLRKVAKAGPYSMFCKLLNMWR
                     DSYTPTQVAEKVKLFFSKYSMNRHKMTTLTPAYHAENYSPDDNRFDLRPFLYNTRWPW
                     QFLCIDNQVLQLERKASQTREEQVLEHFKEPSPIWKQLLPKDP"
     exon            195..255
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            256..372
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            373..426
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            427..516
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            517..568
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            569..657
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            658..775
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            776..907
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            908..982
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1107
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1156
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1259
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1428
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1564
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1671
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1803
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1873
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2002
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2179
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2645
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2378..2383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /note="hexamer: TATAAA"
     regulatory      2382..2387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /note="hexamer: AACAAG"
     polyA_site      2389
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /note="major polyA site"
     polyA_site      2402
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
     regulatory      2623..2628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2645
                     /gene="Nadsyn1"
                     /gene_synonym="9130012B15Rik"
ORIGIN      
        1 aggggcctct aggaaaccaa gctacgcagg cgtagaccgg acagtccggc tagtatcttc
       61 cctgtggtct ccgttattac accgtcccag cctggaacct gtaaccagca tgggccggaa
      121 agtgaccgtg gccacctgtg cactcaatca gtgggccctg gactttgagg gcaatttcca
      181 gaggatttta aagagtattc aaattgccaa aggcaaaggt gcaagataca ggcttggacc
      241 agaactagaa atatgcggct atggatgttg ggatcattat cacgaatcag acactctcct
      301 gcattcgctc caagttctgg ctgctcttct ggactctccg gtcactcagg acatcatctg
      361 tgatgtgggg atgcctataa tgcaccggaa tgttcgctac aactgcagag tcatctttct
      421 caacaggaag atcctgctca tcagacccaa gatggccttg gccaacgaag gcaactaccg
      481 ggaactgcgc tggtttaccc cctggaccag gagccgacaa actgaggaat atgtcctccc
      541 tcggatgtta caggacctga caaagcagaa aactgtgccc tttggagatg tggtactggc
      601 cacccaagac acctgtgtcg ggagtgagat ctgtgaggag ctctggacac cacgcagccc
      661 ccacatcgac atgggcctgg atggtgtgga gatcattacc aatgcctcgg gcagccacca
      721 cgtgctccgc aaagcccata ccagagtgga tctggtaacc atggccacct ccaagaatgg
      781 tggcatctac ctgctggcca accagaaggg ctgtgatggt gaccgtctct actacgatgg
      841 ctgtgccatg attgctatga acgggagcat ctttgcccag ggcactcagt tctcattgga
      901 tgatgtggag gttctcactg ccaccctgga cctggaggac gttaggagct acaaggcaga
      961 gatctcatct cggaacctgg aggcaaccag ggtgagccca tatccccggg tgacggtgga
     1021 ctttgccctt tcggtcagtg aagacttgtt ggagccagtg tcagagccta tggaatggac
     1081 ataccacagg cctgaggagg agataagcct cggccctgcc tgctggctct gggacttcct
     1141 tcgacggagc aaacaggccg ggtttttcct tcccctgagt ggtggtgtgg acagtgcagc
     1201 cagcgcctgt atcgtctact ccatgtgctg cctggtctgc gatgctgtga agagcggaaa
     1261 tcagcaagtg ctgacagacg tccagaacct ggtggacgag agcagctaca cccctcagga
     1321 tccccgggag ctctgcggac ggctgctcac tacttgttac atggccagcg agaactcctc
     1381 ccaggagacc cacagcagag ccacaaaact ggctcagctg attggaagct accacatcaa
     1441 tctgagcatc gacactgctg tgaaggctgt cttgggcatc ttcagcctga tgaccgggaa
     1501 gttgcctcgg ttctcagccc acggagggag cagcagggag aacctggcct tgcagaatgt
     1561 gcaggctcgg attaggatgg tccttgccta cctgtttgct cagctgagcc tctggtctcg
     1621 gggtgctcga ggaagccttc ttgtgcttgg atctgccaat gtggatgaga gtctcctcgg
     1681 ctacctgacc aagtacgact gctccagtgc ggacatcaac cccataggcg gcatcagcaa
     1741 gacggacctg agagcctttg tccagttttg cgcagagcgc ttccagcttc ctgttctgca
     1801 gaccatcctg tcagcaccgg ccactgcaga gctggagccc ttagctgatg ggcaggtgtc
     1861 tcagatggat gaggaagaca tggggatgac gtacgcagaa ctctccatct tcggcaggct
     1921 ccggaaggtc gccaaggcag gcccctacag catgttctgc aaactgctca acatgtggag
     1981 agacagctat acaccaacac aggtagctga gaaggtgaag ctatttttct caaagtactc
     2041 catgaacagg cacaagatga caaccctcac accagcatat catgctgaaa actacagtcc
     2101 tgacgacaac aggtttgacc tgagaccctt tctgtataac acgagatggc cctggcagtt
     2161 cctctgcatt gacaaccagg ttctacagct tgagaggaaa gcatcgcaga cccgggaaga
     2221 acaggtcctg gagcacttca aagagccatc acctatatgg aaacagctcc tccccaagga
     2281 cccctaatgt gaatccagat cagcggtggc cggcacagcc tgaccagatc ttagttacct
     2341 tttaaggaag agagatttat tctaatgaca taaaaaatat aaacaagaat gttcttttta
     2401 aagtgtattt tattattata tatatatagt ctcttgctta catatatgtc tgtgtcctac
     2461 atgcatgcct ggtacccaca gaagccagaa gagggcatta ggtcctctgg gactagagct
     2521 aaagatgatt gtgggtactg ggaatcggac ttgggtcctc tgtgagagca gccagtgtgg
     2581 cttcacaggc atgcgtgtag gcaaaaaagc catgcacgta aaaataaaac tcataaaatt
     2641 ctaaaaaaaa aaaaaaaaaa
//
